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The map label for this gene is pflB [H]

Identifier: 213692583

GI number: 213692583

Start: 1877314

End: 1879722

Strand: Reverse

Name: pflB [H]

Synonym: Blon_1715

Alternate gene names: 213692583

Gene position: 1879722-1877314 (Counterclockwise)

Preceding gene: 213692584

Following gene: 213692582

Centisome position: 66.36

GC content: 63.14

Gene sequence:

>2409_bases
ATGCGAAACCTACAACCCCATGTAAGGAGTGACATGACCGCAGTGGAAAATGCAGCTGTCTCCCAGGAGGAGCTCGACGC
CAAGGCGTGGGCTGGCTTCACCGAGGGCAACTGGCAGAAGGATATCGACGTTCGAGACTTCATCCAGAAGAACTACACCC
CGTACGAGGGCGACGAGAGCTTCCTGGCGCCTGCCACCGAGAAGACCAAGCATCTGTGGAAGTACCTCGACGACAACTAT
CTGGCCGTGGAGCGCAAGCAGCGCGTCTACGACGTTGACACCCACACCCCGGCAGGCATCGACGCCTTCCCGGCCGGCTA
CATCGAATCTCCGGAAATCGACGACGTGATCGTCGGCCTGCAGACCGACGTGCCCTGCAAGCGCGCCATGATGCCGAACG
GCGGCTGGCGCATGGTCGAGCAGGCCATCAAGGAAGCCGGCAAGGAGCCCGATCCGGAGATCAAGAAGATCTTCACCAAG
TACCGCAAGACGCACAACGACGGCGTCTTCGGCGTCTACACCAAGGCCATCAAGATCGCCCGCCACAACAAGATCCTCAC
CGGCCTGCCGGACGCCTACGGCCGAGGCCGCATCATCGGCGACTACCGCCGCGTCGCCCTGTACGGCGTCAACAAGCTGA
TCGCCTTCAAGAAGCGCGATAAGGACTCCATCCCGTACCGCAACGACTTCACCGAGCCGGAGATCGAGCACTGGATCCGC
TTCCGCGAGGAGCACGACGAGCAGATCAAGGCCCTCAAGCAGCTCATCACCCTCGGCAACGAGTACGGCCTGGATCTGAC
CCGTCCGGCCCAGACCGCCAAGGAAGCCGTGCAGTGGACCTACATGGGCTACCTGGCCTCCATCAAGTCCCAGGACGGCG
CCGCCATGTCGTTCGGCCGCAACTCCGCCTTCCTCGACTGCTTCATCGAGCGCGACCTCAAGGCCGGCAAGATCACCGAG
ACCGACGCCCAGGAGCTCATCGACAACATCGTCATGAAGCTGCGCATCGTGCGCTTCCTGCGTACCAAGGACTACGACAA
CATCTTCTCCGGCGACCCGTACTGGGCGACCTGGTCCGACGCCGGTTTCGGCGACGACGGCCGTCCGCTGGTCACCAAGA
CCTCCTTCCGTCTGCTCAACACGCTGACCCTCGAACACCTCGGGCCCGGCCCCGAGCCGAACATCACCATCTTCTGGGAT
CCGAAGCTGCCGGAAGGCTACAAGCGCTTCTGCGCCAAGATCTCCATCGACACCTCCGCCATCCAGTACGAGTCCGACAA
GGAGATCCGCAACCACTGGGGCGATGACGCCGCCATCGCCTGCTGCGTCTCCCCGATGCGTGTGGGCAAGCAGATGCAGT
TCTTCGCCGCCCGAGTGAACTCCGCCAAGGCCCTGCTGTACGCCATCAACGGCGGCCGCGACGAGATGACCGGCATGCAG
GTCATCGACAAGGGCGTCATCGACCCGATCGCCCCGGAAGCCGACGGCACCTTGGACTACGAGAAGGTCAAGAACAACTA
CGAGAAGGCGCTCGAGTGGCTGTCCGAAACCTACGTCGAAGCGCTGAACATCATCCACTACATGCACGATAAGTACGCCT
ACGAGTCCATCGAGATGGCCCTGCACGACAAGGAGGTGTACCGCACCCTCGGTTGCGGCATGTCCGGTCTGTCCATCGCC
GCCGACTCCCTGTCCGCCATCAAGTACGCCAAGGTGTACCCGATCACCAACAAGGAAGCCAAGAACCTCGAAGGCCACGA
GTACGAGTACGTCGAAGGCGCCGACGACGATCTGGTCGTCGGCTACCGCACCGAGGGCGAGTTCCCGGTCTACGGCAACG
ACGACGACCGCGCCGACGACATCGCCAAGTGGGTCGTCTCCACCGTGATGGGCCAGGTCAAGCGCCTGCCCGTCTACCGT
GGCGCCGTCCCGACCCAGTCCATCCTGACCATCACCTCCAACGTGGAGTACGGCAAGAACACCGGTTCCTTCCCGTCCCG
CCACAAGAAGGGCACCCCGTACGCCCCGGGCGCCAACCCGGAGAACGGCATGGACTCGCACGGCATGTTGCCGTCCATGT
TCTCCGTCGGCAAGATCGACTACAACGACGCGCTCGACGGCATCTCGCTGACCAACACCATCACCCCCGATGGCCTGGGC
CGCGATGAAGCCGAGCGCATCGGCAACCTGGTCGGCATCCTGGACGCGGGCAACGGCCACGGCCTCTACCACGCGAACAT
CAACGTTCTGCGCCGCGAGACCATGGAGGACGCCGTCGAGCACCCCGAAAAGTACCCGCACCTGACCGTGCGCGTCTCCG
GCTACGCGGTGAACTTCGTCAAGCTCACCAAGGAGCAGCAGCTCGACGTCATCTCCCGTACCTTCCACCAGGGCGCCGTC
GTCGACTGA

Upstream 100 bases:

>100_bases
ATGGTAGCGTTCGCAAAAACAATGTGAGATAGCTCACCCCCAATTTCGGCTTTTCTCAAGCAAGTGCCAACTACACTTGT
GAACCAAGGGTTCCGTGAGG

Downstream 100 bases:

>100_bases
TGACCAACCCCGCGAACCGCGGCAATGACTAACTGAAGGGGTGGGGCACGGCTCCACCCCTTCCTTATTTTCCACAGACT
TTCGCATCATTTGGAACAGG

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 802; Mature: 802

Protein sequence:

>802_residues
MRNLQPHVRSDMTAVENAAVSQEELDAKAWAGFTEGNWQKDIDVRDFIQKNYTPYEGDESFLAPATEKTKHLWKYLDDNY
LAVERKQRVYDVDTHTPAGIDAFPAGYIESPEIDDVIVGLQTDVPCKRAMMPNGGWRMVEQAIKEAGKEPDPEIKKIFTK
YRKTHNDGVFGVYTKAIKIARHNKILTGLPDAYGRGRIIGDYRRVALYGVNKLIAFKKRDKDSIPYRNDFTEPEIEHWIR
FREEHDEQIKALKQLITLGNEYGLDLTRPAQTAKEAVQWTYMGYLASIKSQDGAAMSFGRNSAFLDCFIERDLKAGKITE
TDAQELIDNIVMKLRIVRFLRTKDYDNIFSGDPYWATWSDAGFGDDGRPLVTKTSFRLLNTLTLEHLGPGPEPNITIFWD
PKLPEGYKRFCAKISIDTSAIQYESDKEIRNHWGDDAAIACCVSPMRVGKQMQFFAARVNSAKALLYAINGGRDEMTGMQ
VIDKGVIDPIAPEADGTLDYEKVKNNYEKALEWLSETYVEALNIIHYMHDKYAYESIEMALHDKEVYRTLGCGMSGLSIA
ADSLSAIKYAKVYPITNKEAKNLEGHEYEYVEGADDDLVVGYRTEGEFPVYGNDDDRADDIAKWVVSTVMGQVKRLPVYR
GAVPTQSILTITSNVEYGKNTGSFPSRHKKGTPYAPGANPENGMDSHGMLPSMFSVGKIDYNDALDGISLTNTITPDGLG
RDEAERIGNLVGILDAGNGHGLYHANINVLRRETMEDAVEHPEKYPHLTVRVSGYAVNFVKLTKEQQLDVISRTFHQGAV
VD

Sequences:

>Translated_802_residues
MRNLQPHVRSDMTAVENAAVSQEELDAKAWAGFTEGNWQKDIDVRDFIQKNYTPYEGDESFLAPATEKTKHLWKYLDDNY
LAVERKQRVYDVDTHTPAGIDAFPAGYIESPEIDDVIVGLQTDVPCKRAMMPNGGWRMVEQAIKEAGKEPDPEIKKIFTK
YRKTHNDGVFGVYTKAIKIARHNKILTGLPDAYGRGRIIGDYRRVALYGVNKLIAFKKRDKDSIPYRNDFTEPEIEHWIR
FREEHDEQIKALKQLITLGNEYGLDLTRPAQTAKEAVQWTYMGYLASIKSQDGAAMSFGRNSAFLDCFIERDLKAGKITE
TDAQELIDNIVMKLRIVRFLRTKDYDNIFSGDPYWATWSDAGFGDDGRPLVTKTSFRLLNTLTLEHLGPGPEPNITIFWD
PKLPEGYKRFCAKISIDTSAIQYESDKEIRNHWGDDAAIACCVSPMRVGKQMQFFAARVNSAKALLYAINGGRDEMTGMQ
VIDKGVIDPIAPEADGTLDYEKVKNNYEKALEWLSETYVEALNIIHYMHDKYAYESIEMALHDKEVYRTLGCGMSGLSIA
ADSLSAIKYAKVYPITNKEAKNLEGHEYEYVEGADDDLVVGYRTEGEFPVYGNDDDRADDIAKWVVSTVMGQVKRLPVYR
GAVPTQSILTITSNVEYGKNTGSFPSRHKKGTPYAPGANPENGMDSHGMLPSMFSVGKIDYNDALDGISLTNTITPDGLG
RDEAERIGNLVGILDAGNGHGLYHANINVLRRETMEDAVEHPEKYPHLTVRVSGYAVNFVKLTKEQQLDVISRTFHQGAV
VD
>Mature_802_residues
MRNLQPHVRSDMTAVENAAVSQEELDAKAWAGFTEGNWQKDIDVRDFIQKNYTPYEGDESFLAPATEKTKHLWKYLDDNY
LAVERKQRVYDVDTHTPAGIDAFPAGYIESPEIDDVIVGLQTDVPCKRAMMPNGGWRMVEQAIKEAGKEPDPEIKKIFTK
YRKTHNDGVFGVYTKAIKIARHNKILTGLPDAYGRGRIIGDYRRVALYGVNKLIAFKKRDKDSIPYRNDFTEPEIEHWIR
FREEHDEQIKALKQLITLGNEYGLDLTRPAQTAKEAVQWTYMGYLASIKSQDGAAMSFGRNSAFLDCFIERDLKAGKITE
TDAQELIDNIVMKLRIVRFLRTKDYDNIFSGDPYWATWSDAGFGDDGRPLVTKTSFRLLNTLTLEHLGPGPEPNITIFWD
PKLPEGYKRFCAKISIDTSAIQYESDKEIRNHWGDDAAIACCVSPMRVGKQMQFFAARVNSAKALLYAINGGRDEMTGMQ
VIDKGVIDPIAPEADGTLDYEKVKNNYEKALEWLSETYVEALNIIHYMHDKYAYESIEMALHDKEVYRTLGCGMSGLSIA
ADSLSAIKYAKVYPITNKEAKNLEGHEYEYVEGADDDLVVGYRTEGEFPVYGNDDDRADDIAKWVVSTVMGQVKRLPVYR
GAVPTQSILTITSNVEYGKNTGSFPSRHKKGTPYAPGANPENGMDSHGMLPSMFSVGKIDYNDALDGISLTNTITPDGLG
RDEAERIGNLVGILDAGNGHGLYHANINVLRRETMEDAVEHPEKYPHLTVRVSGYAVNFVKLTKEQQLDVISRTFHQGAV
VD

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=778, Percent_Identity=54.6272493573265, Blast_Score=822, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=784, Percent_Identity=52.0408163265306, Blast_Score=793, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=579, Percent_Identity=24.006908462867, Blast_Score=132, Evalue=1e-31,
Organism=Escherichia coli, GI1790388, Length=675, Percent_Identity=24.7407407407407, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1788933, Length=59, Percent_Identity=69.4915254237288, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 90264; Mature: 90264

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNLQPHVRSDMTAVENAAVSQEELDAKAWAGFTEGNWQKDIDVRDFIQKNYTPYEGDES
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCC
FLAPATEKTKHLWKYLDDNYLAVERKQRVYDVDTHTPAGIDAFPAGYIESPEIDDVIVGL
CCCCCHHHHHHHHHHHCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEC
QTDVPCKRAMMPNGGWRMVEQAIKEAGKEPDPEIKKIFTKYRKTHNDGVFGVYTKAIKIA
CCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHH
RHNKILTGLPDAYGRGRIIGDYRRVALYGVNKLIAFKKRDKDSIPYRNDFTEPEIEHWIR
HCCCCEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
FREEHDEQIKALKQLITLGNEYGLDLTRPAQTAKEAVQWTYMGYLASIKSQDGAAMSFGR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCC
NSAFLDCFIERDLKAGKITETDAQELIDNIVMKLRIVRFLRTKDYDNIFSGDPYWATWSD
CCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCC
AGFGDDGRPLVTKTSFRLLNTLTLEHLGPGPEPNITIFWDPKLPEGYKRFCAKISIDTSA
CCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHEEECCHH
IQYESDKEIRNHWGDDAAIACCVSPMRVGKQMQFFAARVNSAKALLYAINGGRDEMTGMQ
EEECCCHHHHHCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHH
VIDKGVIDPIAPEADGTLDYEKVKNNYEKALEWLSETYVEALNIIHYMHDKYAYESIEMA
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHDKEVYRTLGCGMSGLSIAADSLSAIKYAKVYPITNKEAKNLEGHEYEYVEGADDDLVV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCEEEECCCCCCEEE
GYRTEGEFPVYGNDDDRADDIAKWVVSTVMGQVKRLPVYRGAVPTQSILTITSNVEYGKN
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCC
TGSFPSRHKKGTPYAPGANPENGMDSHGMLPSMFSVGKIDYNDALDGISLTNTITPDGLG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEECCCCCCCCCC
RDEAERIGNLVGILDAGNGHGLYHANINVLRRETMEDAVEHPEKYPHLTVRVSGYAVNFV
CHHHHHHCCEEEEEECCCCCEEEECCHHHHHHHHHHHHHHCHHHCCEEEEEEECEEEEEE
KLTKEQQLDVISRTFHQGAVVD
EECCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRNLQPHVRSDMTAVENAAVSQEELDAKAWAGFTEGNWQKDIDVRDFIQKNYTPYEGDES
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCC
FLAPATEKTKHLWKYLDDNYLAVERKQRVYDVDTHTPAGIDAFPAGYIESPEIDDVIVGL
CCCCCHHHHHHHHHHHCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEC
QTDVPCKRAMMPNGGWRMVEQAIKEAGKEPDPEIKKIFTKYRKTHNDGVFGVYTKAIKIA
CCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHH
RHNKILTGLPDAYGRGRIIGDYRRVALYGVNKLIAFKKRDKDSIPYRNDFTEPEIEHWIR
HCCCCEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
FREEHDEQIKALKQLITLGNEYGLDLTRPAQTAKEAVQWTYMGYLASIKSQDGAAMSFGR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCC
NSAFLDCFIERDLKAGKITETDAQELIDNIVMKLRIVRFLRTKDYDNIFSGDPYWATWSD
CCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCC
AGFGDDGRPLVTKTSFRLLNTLTLEHLGPGPEPNITIFWDPKLPEGYKRFCAKISIDTSA
CCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHEEECCHH
IQYESDKEIRNHWGDDAAIACCVSPMRVGKQMQFFAARVNSAKALLYAINGGRDEMTGMQ
EEECCCHHHHHCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHH
VIDKGVIDPIAPEADGTLDYEKVKNNYEKALEWLSETYVEALNIIHYMHDKYAYESIEMA
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHDKEVYRTLGCGMSGLSIAADSLSAIKYAKVYPITNKEAKNLEGHEYEYVEGADDDLVV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCEEEECCCCCCEEE
GYRTEGEFPVYGNDDDRADDIAKWVVSTVMGQVKRLPVYRGAVPTQSILTITSNVEYGKN
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCC
TGSFPSRHKKGTPYAPGANPENGMDSHGMLPSMFSVGKIDYNDALDGISLTNTITPDGLG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEECCCCCCCCCC
RDEAERIGNLVGILDAGNGHGLYHANINVLRRETMEDAVEHPEKYPHLTVRVSGYAVNFV
CHHHHHHCCEEEEEECCCCCEEEECCHHHHHHHHHHHHHHCHHHCCEEEEEEECEEEEEE
KLTKEQQLDVISRTFHQGAVVD
EECCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]