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The map label for this gene is act [H]

Identifier: 213692582

GI number: 213692582

Start: 1876323

End: 1877204

Strand: Reverse

Name: act [H]

Synonym: Blon_1714

Alternate gene names: 213692582

Gene position: 1877204-1876323 (Counterclockwise)

Preceding gene: 213692583

Following gene: 213692581

Centisome position: 66.27

GC content: 61.79

Gene sequence:

>882_bases
ATGCCCGCAACACCCACGTTCCGCACCACGACCAGGCATATGCTCAAGGAATCGAAGACCTATGCCTCGCAGACTTTGAT
GGGCGGCCTTTCCGGCTTTGAATCCCCAATCGGACTCGACCGACGTGACCGCCTTTCCGCCCTGAAATCCGGCGATATCG
GCTTCGTCCACTCTTGGGACATCAACACCTCCGTGGATGGACCGGGCACCCGCATGACCGTATTCATGTCCGGCTGCCCG
CTGCGCTGCCAGTACTGCCAGAACCCGGACACTTGGAAGATGCGCGACGGCAAGCCCGTCTACCTTGACGCCATGATCAA
GAAGGTCGATCGTTACAAGGACCTGTTCAAGGCCACGCATGGCGGTATCACCTTCTCCGGCGGCGAATCCATGATGCAGC
CCGCCTTCGTCTCGCGCGTGTTCCACGCCGCCCGGGAGATGGGCGTGCACACCTGCCTCGATACGTCCGGCTTCCTCAAC
ACGAGCTACACCGACGAGATGCTCGAGGACATCGACCTATGCCTGCTCGACGTCAAATCCGGTGACGAACGGACCTATCA
CGAGGTCACCGGCGGCACCCTGCAGCCCACCATCGACTTCGGGCAGCGGTTGGCCAAGGCCGGCAAGAAGATCTGGGTGC
GTTTCGTGCTGGTCCCGGGGTTGACCGACTCCGAGGAGAACGTCGAAAACGTGGCGCGGATCTGCGAGACCTTCGGCGAC
GCCATCGAACACGTCGACGTGCTGGGATTCCACCAGCTGGGCCGGCCGAAGTGGCACGAACTGCGCATCCCATACCCGCT
GGAGAACCAGAAGGGACCCAACGCCGCCACTCGCGAACGGGTGGCCAACCAGTTCAAGGACCACGGCTTCACCGTGTTTT
AA

Upstream 100 bases:

>100_bases
CCGCGAACCGCGGCAATGACTAACTGAAGGGGTGGGGCACGGCTCCACCCCTTCCTTATTTTCCACAGACTTTCGCATCA
TTTGGAACAGGAGGACAACT

Downstream 100 bases:

>100_bases
ATTGCAGGGCCGAACCGCGCGTAAATACCACGATTCGGCCCGTTCGCCCATACCCTAGAGATATGGCCGACATCTACGCA
TTTCCTATGGGAGCGTCCAA

Product: pyruvate formate-lyase activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme; Formate-C-acetyltransferase-activating enzyme [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MPATPTFRTTTRHMLKESKTYASQTLMGGLSGFESPIGLDRRDRLSALKSGDIGFVHSWDINTSVDGPGTRMTVFMSGCP
LRCQYCQNPDTWKMRDGKPVYLDAMIKKVDRYKDLFKATHGGITFSGGESMMQPAFVSRVFHAAREMGVHTCLDTSGFLN
TSYTDEMLEDIDLCLLDVKSGDERTYHEVTGGTLQPTIDFGQRLAKAGKKIWVRFVLVPGLTDSEENVENVARICETFGD
AIEHVDVLGFHQLGRPKWHELRIPYPLENQKGPNAATRERVANQFKDHGFTVF

Sequences:

>Translated_293_residues
MPATPTFRTTTRHMLKESKTYASQTLMGGLSGFESPIGLDRRDRLSALKSGDIGFVHSWDINTSVDGPGTRMTVFMSGCP
LRCQYCQNPDTWKMRDGKPVYLDAMIKKVDRYKDLFKATHGGITFSGGESMMQPAFVSRVFHAAREMGVHTCLDTSGFLN
TSYTDEMLEDIDLCLLDVKSGDERTYHEVTGGTLQPTIDFGQRLAKAGKKIWVRFVLVPGLTDSEENVENVARICETFGD
AIEHVDVLGFHQLGRPKWHELRIPYPLENQKGPNAATRERVANQFKDHGFTVF
>Mature_292_residues
PATPTFRTTTRHMLKESKTYASQTLMGGLSGFESPIGLDRRDRLSALKSGDIGFVHSWDINTSVDGPGTRMTVFMSGCPL
RCQYCQNPDTWKMRDGKPVYLDAMIKKVDRYKDLFKATHGGITFSGGESMMQPAFVSRVFHAAREMGVHTCLDTSGFLNT
SYTDEMLEDIDLCLLDVKSGDERTYHEVTGGTLQPTIDFGQRLAKAGKKIWVRFVLVPGLTDSEENVENVARICETFGDA
IEHVDVLGFHQLGRPKWHELRIPYPLENQKGPNAATRERVANQFKDHGFTVF

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=243, Percent_Identity=36.6255144032922, Blast_Score=184, Evalue=4e-48,
Organism=Escherichia coli, GI1790389, Length=251, Percent_Identity=26.6932270916335, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI226510931, Length=166, Percent_Identity=31.3253012048193, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 32879; Mature: 32748

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPATPTFRTTTRHMLKESKTYASQTLMGGLSGFESPIGLDRRDRLSALKSGDIGFVHSWD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECC
INTSVDGPGTRMTVFMSGCPLRCQYCQNPDTWKMRDGKPVYLDAMIKKVDRYKDLFKATH
CCCCCCCCCCEEEEEECCCCCEEHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHC
GGITFSGGESMMQPAFVSRVFHAAREMGVHTCLDTSGFLNTSYTDEMLEDIDLCLLDVKS
CCEEECCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHEEEEECCC
GDERTYHEVTGGTLQPTIDFGQRLAKAGKKIWVRFVLVPGLTDSEENVENVARICETFGD
CCCCHHHHHCCCCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHH
AIEHVDVLGFHQLGRPKWHELRIPYPLENQKGPNAATRERVANQFKDHGFTVF
HHHHHHHHHHHHCCCCCHHEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PATPTFRTTTRHMLKESKTYASQTLMGGLSGFESPIGLDRRDRLSALKSGDIGFVHSWD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECC
INTSVDGPGTRMTVFMSGCPLRCQYCQNPDTWKMRDGKPVYLDAMIKKVDRYKDLFKATH
CCCCCCCCCCEEEEEECCCCCEEHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHC
GGITFSGGESMMQPAFVSRVFHAAREMGVHTCLDTSGFLNTSYTDEMLEDIDLCLLDVKS
CCEEECCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHEEEEECCC
GDERTYHEVTGGTLQPTIDFGQRLAKAGKKIWVRFVLVPGLTDSEENVENVARICETFGD
CCCCHHHHHCCCCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHH
AIEHVDVLGFHQLGRPKWHELRIPYPLENQKGPNAATRERVANQFKDHGFTVF
HHHHHHHHHHHHCCCCCHHEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8636053 [H]