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The map label for this gene is pcrA [H]

Identifier: 213692487

GI number: 213692487

Start: 1766760

End: 1769453

Strand: Reverse

Name: pcrA [H]

Synonym: Blon_1616

Alternate gene names: 213692487

Gene position: 1769453-1766760 (Counterclockwise)

Preceding gene: 213692490

Following gene: 213692486

Centisome position: 62.46

GC content: 62.62

Gene sequence:

>2694_bases
GTGTACGACGAGCCGGAAGTCATTGCACGCAGCGCGCAGGAACTTGTAGGCGATCTGAACCCGCAGCAGGCGGAAGCCGT
GCAATATCGCGGTCAGGCGCTGCTGATCGGCGCCGGTGCCGGCTCCGGCAAAACCCGCGTGCTGACCCGCCGCATCGCCT
GGATCTTGAGCCAGTTCGGCGCATGGCCGAGCCAGATTCTGGCCATCACCTTCACCAACAAGGCCGCCGCCGAAATGCGC
GAACGACTCGGTTCGCTGATCGGCCCGGTGGCCCAGCGCATGTGGGTCTCCACCTTCCATTCCGCCTGCGTGCGCATTCT
GAGGCGCGACGGCAAGTCCATCGGGCTCAAGTCCGGCTTCTCCATTTATGATTCCGCCGACTCCGAGCGACTCGTCAAAA
TCATCGCCACCGAATTCAACCTCGACATCAAGCGCTACACTCCACGCTCGATCCTCGGCCATATCTCCGACCTCAAGAAC
AATCTGACCGGTTGGAAGGAAAACCTCGCCGTTCACGCGCCCGACTTCACGCCTGGCCAGCGCGGCTACCAGTTCGGCAC
GGTGGGCGACCTTGAAGCCATCTATGCGGTGATCTACGCCGAATACGAGCATCGCTTGGCCCTGGCCAACGCCGTCGACT
TCGACGATCTGATCGGCCGCACGGTCGAGTTGCTGCGCACCGACCCGGCCGTCGCCGAGTACTACCATCACCGCTTCCGT
TACATTCTCGTCGACGAGTATCAGGACACCAACCACGCCCAGTACGTGCTTGTGCGCGAGCTGGCCGGTGTGGACACGGG
GGAGAAGGCCATCCCGGGCGCGCCGAACGCCGGCAAGTCCGGCCCCGCCTGGATCACCGTGGTGGGCGATTCCGACCAGT
CCATCTACGCGTTCCGTGGCGCCGACATCCGCAACATCCAGGACTTCGAGCAGGACTTCCCGAACGCCAAGACCATCATG
CTCGAACAGAACTATCGTTCGACGCAGACCATTCTGGATGCGGCCAACGCGGTGATCAGCAACAACGAGGGCCGCAAGCC
CAAGAAACTGTGGACCGCGCTCGGCAAGGGCGAGCCGATTGTCGGCTACGCGGCGGACAACGCCCAGCAGGAGGCCCAGT
GGGTAGCCACCGAAATCGCGCGCCTGCACGCCGAAGAGGGCATCGCCTACTCCGACATGGCGATCATGTACCGCGCCAAC
GCCCAATCTCGCTCGCTCGAAGAGGCGCTGATCAACACGAATCAGCCGTATCAGCTCGTCGGCGGCACCAAGTTCTACGA
ACGTCGCGAAATCAAGGATGCGCTGGCCTATTTGCAGGCGCTCGTCAATCCGGACGACGATGTGAATCTACGTCGTATTC
TCAACGTGCCCAAGCGCGGGCTTGGCGACCGCGCCGAAGGCGTGCTGCTCGCCTACGCGCGCGAGCATGGCACCAGCTTC
TTCTACGCACTCATGCACTTGGATGAGATCGAGGTGCCGACCCGCACCGCTACCTCGCTACGAGCATTCCGCGATCTGAT
GGGCGCGCTCTCGCAGTTCACGCACGCGCACGACTCCAAGCCCAGCGAGATCGTGGCCGAAGTATTGGAGAAGTCGGGCT
TAAGGGCCGAACTTGAGAAATCCGTGGATCCGCAGGACGCCTCCCGTCTGGAGAACCTCTCCCAGCTGCAGTCCACCGCA
GCCGAATTCGAACAGAACACGCCTGACGCCACGTTGTCCGCATTCCTGGAGACCACTGCGCTCGTGGCCGACTCCGACCA
GCTGCCCGGTGAGGCGGAGGACTCCGGCAAGGTGACGCTCATGACCTTGCACACCGCCAAGGGCCTCGAATACCCGGTGG
TGTTCCTGACCGGCATGGAACAGGGCACCTTCCCGCATTCGCGTTCGATGGAAGACACCACTGAACTGCAGGAGGAACGC
CGACTCGCCTATGTGGGCATCACGCGTGCCAAGCAGAGACTGTATGTCACGCGCGCCGCCGTCCGCTCCCAGTGGGGCCA
GGCCGCCGATATGATGCCCAGCCAATTCCTCGACGAGATTCCGGATTCGCTGATTGATTGGAAACGCCGTGAAGCCGGCG
TGGAGCGTATGCGCGCCAACTGGGAGACCGATGGATTCGCCGACGATTTGGGCGGCTGGGATGATGACGATTTCGGCGGT
GCCACGTTTGGCGGGTCGTCGACGTTCGGCTCGCGTGGTTCGTCCGGTTCGGGTTCGTCCTATGGGTCGCGGTCGCGGTA
TGGGTCGTCGTATGGCTCGGGTTCCGGCTCCTCGTCGTATGGTTCCCGTTCAGGTTCTTCGTACGGTTCCCGTTCATCAT
CATATGATTCCCGCTCCGGCTCGTCCTCCTACGGGTCTCGCTCCCGTTCGGGGTCGTCTTACGGGCCGCGTTCCCGTTCC
GGTTCTTCGTCCGGTTCATATGGCGGTACCCGTGGCGGCAAGGTCACCACGCGCCGCACCACACCGAAATCCGGTTCGAC
CGGCTCTGCCGTGCCCAGCTCGAAGCTCACCAAGGACAATGGCCTGAATATCGCCGACTTCGCCGTGGGCGACATGATCA
GCCATGATCAGTACGGTCTCGGCAAGGTCACCGACGCGCAAGACAAGGGTCGCAATTCGGTAATCACCGTGGACTTCGGC
TCCGCCGGCGTCAAACGCCTCATGCTCCGCGTAGCACCTATTGAGAAGTTGTAG

Upstream 100 bases:

>100_bases
GTATGTCAGTGAGTGATATGGATACTCGTAAGTGTACGTTGTCCTACGCTGACCCGTGCATGTGGTAAACATGAATGTCG
CGATTCAAAGGGAGGCAACA

Downstream 100 bases:

>100_bases
ATAGATTGGCCCCTCTGACGAGGGGCTGAGCTATATACCAAATAAATTCACAAAAAACAAGACTTGTGTTCTTCATCACT
ATCTGGGCATAATGCGGAGG

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 897; Mature: 897

Protein sequence:

>897_residues
MYDEPEVIARSAQELVGDLNPQQAEAVQYRGQALLIGAGAGSGKTRVLTRRIAWILSQFGAWPSQILAITFTNKAAAEMR
ERLGSLIGPVAQRMWVSTFHSACVRILRRDGKSIGLKSGFSIYDSADSERLVKIIATEFNLDIKRYTPRSILGHISDLKN
NLTGWKENLAVHAPDFTPGQRGYQFGTVGDLEAIYAVIYAEYEHRLALANAVDFDDLIGRTVELLRTDPAVAEYYHHRFR
YILVDEYQDTNHAQYVLVRELAGVDTGEKAIPGAPNAGKSGPAWITVVGDSDQSIYAFRGADIRNIQDFEQDFPNAKTIM
LEQNYRSTQTILDAANAVISNNEGRKPKKLWTALGKGEPIVGYAADNAQQEAQWVATEIARLHAEEGIAYSDMAIMYRAN
AQSRSLEEALINTNQPYQLVGGTKFYERREIKDALAYLQALVNPDDDVNLRRILNVPKRGLGDRAEGVLLAYAREHGTSF
FYALMHLDEIEVPTRTATSLRAFRDLMGALSQFTHAHDSKPSEIVAEVLEKSGLRAELEKSVDPQDASRLENLSQLQSTA
AEFEQNTPDATLSAFLETTALVADSDQLPGEAEDSGKVTLMTLHTAKGLEYPVVFLTGMEQGTFPHSRSMEDTTELQEER
RLAYVGITRAKQRLYVTRAAVRSQWGQAADMMPSQFLDEIPDSLIDWKRREAGVERMRANWETDGFADDLGGWDDDDFGG
ATFGGSSTFGSRGSSGSGSSYGSRSRYGSSYGSGSGSSSYGSRSGSSYGSRSSSYDSRSGSSSYGSRSRSGSSYGPRSRS
GSSSGSYGGTRGGKVTTRRTTPKSGSTGSAVPSSKLTKDNGLNIADFAVGDMISHDQYGLGKVTDAQDKGRNSVITVDFG
SAGVKRLMLRVAPIEKL

Sequences:

>Translated_897_residues
MYDEPEVIARSAQELVGDLNPQQAEAVQYRGQALLIGAGAGSGKTRVLTRRIAWILSQFGAWPSQILAITFTNKAAAEMR
ERLGSLIGPVAQRMWVSTFHSACVRILRRDGKSIGLKSGFSIYDSADSERLVKIIATEFNLDIKRYTPRSILGHISDLKN
NLTGWKENLAVHAPDFTPGQRGYQFGTVGDLEAIYAVIYAEYEHRLALANAVDFDDLIGRTVELLRTDPAVAEYYHHRFR
YILVDEYQDTNHAQYVLVRELAGVDTGEKAIPGAPNAGKSGPAWITVVGDSDQSIYAFRGADIRNIQDFEQDFPNAKTIM
LEQNYRSTQTILDAANAVISNNEGRKPKKLWTALGKGEPIVGYAADNAQQEAQWVATEIARLHAEEGIAYSDMAIMYRAN
AQSRSLEEALINTNQPYQLVGGTKFYERREIKDALAYLQALVNPDDDVNLRRILNVPKRGLGDRAEGVLLAYAREHGTSF
FYALMHLDEIEVPTRTATSLRAFRDLMGALSQFTHAHDSKPSEIVAEVLEKSGLRAELEKSVDPQDASRLENLSQLQSTA
AEFEQNTPDATLSAFLETTALVADSDQLPGEAEDSGKVTLMTLHTAKGLEYPVVFLTGMEQGTFPHSRSMEDTTELQEER
RLAYVGITRAKQRLYVTRAAVRSQWGQAADMMPSQFLDEIPDSLIDWKRREAGVERMRANWETDGFADDLGGWDDDDFGG
ATFGGSSTFGSRGSSGSGSSYGSRSRYGSSYGSGSGSSSYGSRSGSSYGSRSSSYDSRSGSSSYGSRSRSGSSYGPRSRS
GSSSGSYGGTRGGKVTTRRTTPKSGSTGSAVPSSKLTKDNGLNIADFAVGDMISHDQYGLGKVTDAQDKGRNSVITVDFG
SAGVKRLMLRVAPIEKL
>Mature_897_residues
MYDEPEVIARSAQELVGDLNPQQAEAVQYRGQALLIGAGAGSGKTRVLTRRIAWILSQFGAWPSQILAITFTNKAAAEMR
ERLGSLIGPVAQRMWVSTFHSACVRILRRDGKSIGLKSGFSIYDSADSERLVKIIATEFNLDIKRYTPRSILGHISDLKN
NLTGWKENLAVHAPDFTPGQRGYQFGTVGDLEAIYAVIYAEYEHRLALANAVDFDDLIGRTVELLRTDPAVAEYYHHRFR
YILVDEYQDTNHAQYVLVRELAGVDTGEKAIPGAPNAGKSGPAWITVVGDSDQSIYAFRGADIRNIQDFEQDFPNAKTIM
LEQNYRSTQTILDAANAVISNNEGRKPKKLWTALGKGEPIVGYAADNAQQEAQWVATEIARLHAEEGIAYSDMAIMYRAN
AQSRSLEEALINTNQPYQLVGGTKFYERREIKDALAYLQALVNPDDDVNLRRILNVPKRGLGDRAEGVLLAYAREHGTSF
FYALMHLDEIEVPTRTATSLRAFRDLMGALSQFTHAHDSKPSEIVAEVLEKSGLRAELEKSVDPQDASRLENLSQLQSTA
AEFEQNTPDATLSAFLETTALVADSDQLPGEAEDSGKVTLMTLHTAKGLEYPVVFLTGMEQGTFPHSRSMEDTTELQEER
RLAYVGITRAKQRLYVTRAAVRSQWGQAADMMPSQFLDEIPDSLIDWKRREAGVERMRANWETDGFADDLGGWDDDDFGG
ATFGGSSTFGSRGSSGSGSSYGSRSRYGSSYGSGSGSSSYGSRSGSSYGSRSSSYDSRSGSSSYGSRSRSGSSYGPRSRS
GSSSGSYGGTRGGKVTTRRTTPKSGSTGSAVPSSKLTKDNGLNIADFAVGDMISHDQYGLGKVTDAQDKGRNSVITVDFG
SAGVKRLMLRVAPIEKL

Specific function: Has Both Atpase And Helicase Activities. Unwinds DNA Duplexes With 3' To 5' Polarity With Respect To The Bound Strand And Initiates Unwinding Most Effectively When A Single-Stranded Region Is Present. Involved In The Post-Incision Events Of Nucleotide Exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=689, Percent_Identity=42.0899854862119, Blast_Score=487, Evalue=1e-138,
Organism=Escherichia coli, GI48994965, Length=695, Percent_Identity=37.6978417266187, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1787196, Length=382, Percent_Identity=26.9633507853403, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6322369, Length=761, Percent_Identity=27.5952693823916, Blast_Score=207, Evalue=4e-54,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 98010; Mature: 98010

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDEPEVIARSAQELVGDLNPQQAEAVQYRGQALLIGAGAGSGKTRVLTRRIAWILSQFG
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHC
AWPSQILAITFTNKAAAEMRERLGSLIGPVAQRMWVSTFHSACVRILRRDGKSIGLKSGF
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SIYDSADSERLVKIIATEFNLDIKRYTPRSILGHISDLKNNLTGWKENLAVHAPDFTPGQ
CHHCCCCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHCCCHHHCCEEECCCCCCCC
RGYQFGTVGDLEAIYAVIYAEYEHRLALANAVDFDDLIGRTVELLRTDPAVAEYYHHRFR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHEE
YILVDEYQDTNHAQYVLVRELAGVDTGEKAIPGAPNAGKSGPAWITVVGDSDQSIYAFRG
EEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECC
ADIRNIQDFEQDFPNAKTIMLEQNYRSTQTILDAANAVISNNEGRKPKKLWTALGKGEPI
CCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCE
VGYAADNAQQEAQWVATEIARLHAEEGIAYSDMAIMYRANAQSRSLEEALINTNQPYQLV
EEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEE
GGTKFYERREIKDALAYLQALVNPDDDVNLRRILNVPKRGLGDRAEGVLLAYAREHGTSF
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEEHHHCCHH
FYALMHLDEIEVPTRTATSLRAFRDLMGALSQFTHAHDSKPSEIVAEVLEKSGLRAELEK
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHH
SVDPQDASRLENLSQLQSTAAEFEQNTPDATLSAFLETTALVADSDQLPGEAEDSGKVTL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEE
MTLHTAKGLEYPVVFLTGMEQGTFPHSRSMEDTTELQEERRLAYVGITRAKQRLYVTRAA
EEEECCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCEEEEEHHHHHHHHHHHHHH
VRSQWGQAADMMPSQFLDEIPDSLIDWKRREAGVERMRANWETDGFADDLGGWDDDDFGG
HHHHHCHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCC
ATFGGSSTFGSRGSSGSGSSYGSRSRYGSSYGSGSGSSSYGSRSGSSYGSRSSSYDSRSG
CCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SSSYGSRSRSGSSYGPRSRSGSSSGSYGGTRGGKVTTRRTTPKSGSTGSAVPSSKLTKDN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCC
GLNIADFAVGDMISHDQYGLGKVTDAQDKGRNSVITVDFGSAGVKRLMLRVAPIEKL
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MYDEPEVIARSAQELVGDLNPQQAEAVQYRGQALLIGAGAGSGKTRVLTRRIAWILSQFG
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHC
AWPSQILAITFTNKAAAEMRERLGSLIGPVAQRMWVSTFHSACVRILRRDGKSIGLKSGF
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SIYDSADSERLVKIIATEFNLDIKRYTPRSILGHISDLKNNLTGWKENLAVHAPDFTPGQ
CHHCCCCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHCCCHHHCCEEECCCCCCCC
RGYQFGTVGDLEAIYAVIYAEYEHRLALANAVDFDDLIGRTVELLRTDPAVAEYYHHRFR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHEE
YILVDEYQDTNHAQYVLVRELAGVDTGEKAIPGAPNAGKSGPAWITVVGDSDQSIYAFRG
EEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECC
ADIRNIQDFEQDFPNAKTIMLEQNYRSTQTILDAANAVISNNEGRKPKKLWTALGKGEPI
CCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCE
VGYAADNAQQEAQWVATEIARLHAEEGIAYSDMAIMYRANAQSRSLEEALINTNQPYQLV
EEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEE
GGTKFYERREIKDALAYLQALVNPDDDVNLRRILNVPKRGLGDRAEGVLLAYAREHGTSF
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEEHHHCCHH
FYALMHLDEIEVPTRTATSLRAFRDLMGALSQFTHAHDSKPSEIVAEVLEKSGLRAELEK
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHH
SVDPQDASRLENLSQLQSTAAEFEQNTPDATLSAFLETTALVADSDQLPGEAEDSGKVTL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEE
MTLHTAKGLEYPVVFLTGMEQGTFPHSRSMEDTTELQEERRLAYVGITRAKQRLYVTRAA
EEEECCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCEEEEEHHHHHHHHHHHHHH
VRSQWGQAADMMPSQFLDEIPDSLIDWKRREAGVERMRANWETDGFADDLGGWDDDDFGG
HHHHHCHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCC
ATFGGSSTFGSRGSSGSGSSYGSRSRYGSSYGSGSGSSSYGSRSGSSYGSRSSSYDSRSG
CCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SSSYGSRSRSGSSYGPRSRSGSSSGSYGGTRGGKVTTRRTTPKSGSTGSAVPSSKLTKDN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCC
GLNIADFAVGDMISHDQYGLGKVTDAQDKGRNSVITVDFGSAGVKRLMLRVAPIEKL
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]