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The map label for this gene is pntAA [H]

Identifier: 213692450

GI number: 213692450

Start: 1729150

End: 1730313

Strand: Reverse

Name: pntAA [H]

Synonym: Blon_1579

Alternate gene names: 213692450

Gene position: 1730313-1729150 (Counterclockwise)

Preceding gene: 213692452

Following gene: 213692449

Centisome position: 61.08

GC content: 65.21

Gene sequence:

>1164_bases
ATGGCGCAAAACCCAGAAGCCACCGTGGTGTTCGGCGTTTTGAACGAAACGTCCGAAACGGAATCCCGCGTCGCGCTGAC
GCCGGATATCGTCACCCGGCTGAAGAGGAGCGGTGTCTCTTCACTGATTGAAGCAGGTGCCGGCATTGCTGCCGACTACA
CCGATGAGGATTACGAAAAGGCCGGTGCGAAGGTGACCAGCCGTGACGAGGTGCTCGCCGAAGCCGACGCGCTCGGTTTT
GTGGACCGACCGTCCGCCGAGACCGTGGCCAAGCTCAGGGCCGGCCAGTGGGTCATTGGCATGCTCGGTTCCTTCACCGA
TGCCGATTACATGGCCGCGCTCGAGAAGGCCGGTCTGGTGGGCATCGCCATCGAAAAGCTGCCCCGTAAGCTTTCCAGCG
CCCAGTCCATGGATGAGATGACCTCTCAGAACTCCGTGATGGGCTACAAGGCAGCCATCACCGCCGCCGATGCTTACGGT
TCCTTCCTGCCGATGATGACCACCGCCGCCGGCACGATTCGCCCGGCTAAGGCGCTGGTGCTCGGTGCCGGCATCGCCGG
CCTGCAGGCCATTGGTACGCTGAAGCGTCTAGGCGCGGTCGTCACCGCGTACGACGTGCGTCCCGCCTCCCAGGGCGAGG
TCGAGTCCCTCGGCGCGAAGTTCCTTGATCTGGGACTTGACTTCTCCAAGGGCCAGGGTGAGGGCGGTTACGCCCGTGCG
CTGACCGCCGAGGAGCAGGCTCAGCAGCAGGCTGCCGTCGACGAGAAGGCCGCCGGCTTTGACATCATCATCACCACCGC
CAAGGTCCCGGGCCGCAAGCCTCCGGTGCTGCTCACCGCAGCCGGTGTCAAGGGTCTCAAGCGTGGTGCCGTGATCGTGG
ACTGCGCAGCATCCGACCTCGGCGGCAACGTCGAGGGCTCCGCCGTCGGCGAGCAGGTCACCGAGGGCGGCGTCAAGCTG
ATCGGTGCCCCTTATCTGGCCTCCGGCGTCGCCACCACTGCGTCCAATCTGCTTTCGCGCAATGTGGCCGACATCCTCGT
GCACTTCGTGCGTGACGGCAAGCTCGGCCAGGATCTCGCCGAAGAACTTGACGACGCGCTGGTGGTTGCCGGCCGCGCCG
CCGCCCCGGCAGCTGCTGCCGATGCCGAGAAGTCCGAGAAGTAA

Upstream 100 bases:

>100_bases
TTTCGATTGGCGGAATCTCAAGTCATGGCCTTTACCTTCCGACTACACTTTCTATGCGGATAACCATGTCCATTTTGAAG
TAGAACAAGAAAGGGCTCTC

Downstream 100 bases:

>100_bases
GGAAGAAGGAATACCGTGCCTACTCTCGTCGTTTCCATTACCCTGTTCGTCCTCGCGCTGCTCATCGGCATCGAGGTCAT
CGGCAAGGTGCCCGCCACCC

Product: alanine dehydrogenase/PNT domain protein

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha 1; Pyridine nucleotide transhydrogenase subunit alpha 1 [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MAQNPEATVVFGVLNETSETESRVALTPDIVTRLKRSGVSSLIEAGAGIAADYTDEDYEKAGAKVTSRDEVLAEADALGF
VDRPSAETVAKLRAGQWVIGMLGSFTDADYMAALEKAGLVGIAIEKLPRKLSSAQSMDEMTSQNSVMGYKAAITAADAYG
SFLPMMTTAAGTIRPAKALVLGAGIAGLQAIGTLKRLGAVVTAYDVRPASQGEVESLGAKFLDLGLDFSKGQGEGGYARA
LTAEEQAQQQAAVDEKAAGFDIIITTAKVPGRKPPVLLTAAGVKGLKRGAVIVDCAASDLGGNVEGSAVGEQVTEGGVKL
IGAPYLASGVATTASNLLSRNVADILVHFVRDGKLGQDLAEELDDALVVAGRAAAPAAAADAEKSEK

Sequences:

>Translated_387_residues
MAQNPEATVVFGVLNETSETESRVALTPDIVTRLKRSGVSSLIEAGAGIAADYTDEDYEKAGAKVTSRDEVLAEADALGF
VDRPSAETVAKLRAGQWVIGMLGSFTDADYMAALEKAGLVGIAIEKLPRKLSSAQSMDEMTSQNSVMGYKAAITAADAYG
SFLPMMTTAAGTIRPAKALVLGAGIAGLQAIGTLKRLGAVVTAYDVRPASQGEVESLGAKFLDLGLDFSKGQGEGGYARA
LTAEEQAQQQAAVDEKAAGFDIIITTAKVPGRKPPVLLTAAGVKGLKRGAVIVDCAASDLGGNVEGSAVGEQVTEGGVKL
IGAPYLASGVATTASNLLSRNVADILVHFVRDGKLGQDLAEELDDALVVAGRAAAPAAAADAEKSEK
>Mature_386_residues
AQNPEATVVFGVLNETSETESRVALTPDIVTRLKRSGVSSLIEAGAGIAADYTDEDYEKAGAKVTSRDEVLAEADALGFV
DRPSAETVAKLRAGQWVIGMLGSFTDADYMAALEKAGLVGIAIEKLPRKLSSAQSMDEMTSQNSVMGYKAAITAADAYGS
FLPMMTTAAGTIRPAKALVLGAGIAGLQAIGTLKRLGAVVTAYDVRPASQGEVESLGAKFLDLGLDFSKGQGEGGYARAL
TAEEQAQQQAAVDEKAAGFDIIITTAKVPGRKPPVLLTAAGVKGLKRGAVIVDCAASDLGGNVEGSAVGEQVTEGGVKLI
GAPYLASGVATTASNLLSRNVADILVHFVRDGKLGQDLAEELDDALVVAGRAAAPAAAADAEKSEK

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=348, Percent_Identity=34.4827586206897, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI122939153, Length=348, Percent_Identity=34.4827586206897, Blast_Score=186, Evalue=2e-47,
Organism=Escherichia coli, GI1787887, Length=361, Percent_Identity=35.4570637119114, Blast_Score=197, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17550456, Length=332, Percent_Identity=39.4578313253012, Blast_Score=223, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR007886 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 39615; Mature: 39484

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQNPEATVVFGVLNETSETESRVALTPDIVTRLKRSGVSSLIEAGAGIAADYTDEDYEK
CCCCCCCEEEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHH
AGAKVTSRDEVLAEADALGFVDRPSAETVAKLRAGQWVIGMLGSFTDADYMAALEKAGLV
HCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHCCCH
GIAIEKLPRKLSSAQSMDEMTSQNSVMGYKAAITAADAYGSFLPMMTTAAGTIRPAKALV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEHHHHHHHHHHHHHHCCCCCCHHHHHH
LGAGIAGLQAIGTLKRLGAVVTAYDVRPASQGEVESLGAKFLDLGLDFSKGQGEGGYARA
EECCHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEE
LTAEEQAQQQAAVDEKAAGFDIIITTAKVPGRKPPVLLTAAGVKGLKRGAVIVDCAASDL
ECHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCCCHHCCCCEEEEECCCCC
GGNVEGSAVGEQVTEGGVKLIGAPYLASGVATTASNLLSRNVADILVHFVRDGKLGQDLA
CCCCCCHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
EELDDALVVAGRAAAPAAAADAEKSEK
HHHHHHEEECCCCCCCCHHCCCCCCCC
>Mature Secondary Structure 
AQNPEATVVFGVLNETSETESRVALTPDIVTRLKRSGVSSLIEAGAGIAADYTDEDYEK
CCCCCCEEEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHH
AGAKVTSRDEVLAEADALGFVDRPSAETVAKLRAGQWVIGMLGSFTDADYMAALEKAGLV
HCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHCCCH
GIAIEKLPRKLSSAQSMDEMTSQNSVMGYKAAITAADAYGSFLPMMTTAAGTIRPAKALV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEHHHHHHHHHHHHHHCCCCCCHHHHHH
LGAGIAGLQAIGTLKRLGAVVTAYDVRPASQGEVESLGAKFLDLGLDFSKGQGEGGYARA
EECCHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEE
LTAEEQAQQQAAVDEKAAGFDIIITTAKVPGRKPPVLLTAAGVKGLKRGAVIVDCAASDL
ECHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCCCHHCCCCEEEEECCCCC
GGNVEGSAVGEQVTEGGVKLIGAPYLASGVATTASNLLSRNVADILVHFVRDGKLGQDLA
CCCCCCHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
EELDDALVVAGRAAAPAAAADAEKSEK
HHHHHHEEECCCCCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9823893; 9335295; 8662004 [H]