Click here to switch to the map view.

The map label for this gene is purH [H]

Identifier: 213692046

GI number: 213692046

Start: 1345483

End: 1347036

Strand: Direct

Name: purH [H]

Synonym: Blon_1164

Alternate gene names: 213692046

Gene position: 1345483-1347036 (Clockwise)

Preceding gene: 213692045

Following gene: 213692048

Centisome position: 47.5

GC content: 64.67

Gene sequence:

>1554_bases
GTGAAGGCCGGCACCGAAGTCGTTTCCACCGGTTCGACCGCCAGGAAGCTCGCCGAGCTGGGCGTCAAGGTAACCGAGGT
GTCCGATGTCACCGGATTCCCCGAATGCCTCGACGGCCGCGTCAAGACGCTGCATCCGTACATTCACGCCGGCATTCTAG
CTGATATGACCAACCCCGAACACGCCAAGCAGCTTGACGGCTTCGGCATCAAGCCGTTTGATCTGGTGGTCGTCAACCTG
TACCCATTCGCCGACACCGTTCGCTCCGGTGCCAACGAAGCCGACACCATCGAGAAGATCGATATCGGCGGCCCCTCCAT
GGTGCGTGGCGCGGCCAAGAACCACGCCACCGTGGCCATCGTCACCGATCCGGCCGATTACGCCTTGGTCGCCGAGCGTG
TGGCCGACGGCACCGGCTTCTCCCTCGACGAGCGCAAGTGGCTTGCCGCCAAGGCATTCGCCCACACCGCCGCCTATGAC
GCCACCATCAACGAATGGACCGCCAAGCACTGGCCGAAGCCGGCTTCACTGGACACCGCCGAAGTGGACAAGGACGATCA
GGACACTGAAATCGATTCCGCCAAGTTCCCGGCACGATTCACCCGCACCTGGGACCGCGCCCACACCCTGCGCTACGGCG
AGAACTCCCACCAGCAGGCCGCCCTCTACATTGACCCGCTCAACCAGACCGGTTTCGCCCACGCCGAGCAGCTCGGCGGC
AAGCCGATGAGCTACAACAACTACGTGGACGCGGACGCCGCCTGGCGCACCGTATGGGATATGGCTCCCGCCATTGCCGT
GGCCGTAGTCAAGCACAACAATCCGTGCGGTCTGGCCATTGGCGCGACCGTGGCCGAGGCCCACAAGAAGGCTCACGCCT
GCGACCCGATGAGCGCCTACGGCGGTGTGATCGCCTGCAACTCCAAGGTCACTCTTGAGATGGCCGAAAGCGTGCGCCCG
ATTTTCACCGAAGTCATCGTCGCCCCCGACTATGAGCCGGCCGCGCTCGAACTGCTGCAGACCAAGAAGAAGAACCTGCG
CATCCTCAAGGTGGCCGAGCCGCCGAAGGGCCACGAGGCCATCCGCCAGATCGATGGCGGCCTGCTTGTGCAGGACACTG
ATCTGATCAACGCCGTCGGCGACGATCCGGACGCCTGGAAGCTCGTGGCCGGCGAGGCCGCCGACGCCGACACGCTGAAG
GACCTCGTGTTCGCATGGCGTGCAATCCGCTGCGTGAAGTCCAACGCCATCCTGCTGGCCCACGACCAGGCCACGGTTGG
CATCGGCATGGGCCAAGTCAACCGCGTGGACTCCTGCCATCTGGCCGTCGAGCGCGCCAACACACTGGCCGATGGTGCCG
ATCGCGCCACCGGTGCGGTCGCGGCATCCGATGCGTTCTTCCCGTTCGCCGATGGTGCCCAGGTGCTTATCGACGCGGGC
GTCAAGGCAATCGTGCAGCCTGGCGGCTCCATCCGCGATGAGGAGGTCATCGAAGCGGCCAATAAGGCCGGCGTGACCAT
GTACCTCACCGGCACCCGCCACTTCTTCCACTGA

Upstream 100 bases:

>100_bases
TCCAAGGAGGAACAGGATGACAAACACGAACCGACCGATCCGTCGGGCACTGGTCTCGGTCTTTCATAAGGAAGGCATCG
AAGTACTCGCGGAAGCGTTC

Downstream 100 bases:

>100_bases
TCGGGACTCTCTGAGGAGAGCAGCTCAGCCAAAACGCACAAAGGTGGGATTCCGCCTGGACCCGTTGACGGGTCCAGGCG
GAATCCCACCTTTGCTATTC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MKAGTEVVSTGSTARKLAELGVKVTEVSDVTGFPECLDGRVKTLHPYIHAGILADMTNPEHAKQLDGFGIKPFDLVVVNL
YPFADTVRSGANEADTIEKIDIGGPSMVRGAAKNHATVAIVTDPADYALVAERVADGTGFSLDERKWLAAKAFAHTAAYD
ATINEWTAKHWPKPASLDTAEVDKDDQDTEIDSAKFPARFTRTWDRAHTLRYGENSHQQAALYIDPLNQTGFAHAEQLGG
KPMSYNNYVDADAAWRTVWDMAPAIAVAVVKHNNPCGLAIGATVAEAHKKAHACDPMSAYGGVIACNSKVTLEMAESVRP
IFTEVIVAPDYEPAALELLQTKKKNLRILKVAEPPKGHEAIRQIDGGLLVQDTDLINAVGDDPDAWKLVAGEAADADTLK
DLVFAWRAIRCVKSNAILLAHDQATVGIGMGQVNRVDSCHLAVERANTLADGADRATGAVAASDAFFPFADGAQVLIDAG
VKAIVQPGGSIRDEEVIEAANKAGVTMYLTGTRHFFH

Sequences:

>Translated_517_residues
MKAGTEVVSTGSTARKLAELGVKVTEVSDVTGFPECLDGRVKTLHPYIHAGILADMTNPEHAKQLDGFGIKPFDLVVVNL
YPFADTVRSGANEADTIEKIDIGGPSMVRGAAKNHATVAIVTDPADYALVAERVADGTGFSLDERKWLAAKAFAHTAAYD
ATINEWTAKHWPKPASLDTAEVDKDDQDTEIDSAKFPARFTRTWDRAHTLRYGENSHQQAALYIDPLNQTGFAHAEQLGG
KPMSYNNYVDADAAWRTVWDMAPAIAVAVVKHNNPCGLAIGATVAEAHKKAHACDPMSAYGGVIACNSKVTLEMAESVRP
IFTEVIVAPDYEPAALELLQTKKKNLRILKVAEPPKGHEAIRQIDGGLLVQDTDLINAVGDDPDAWKLVAGEAADADTLK
DLVFAWRAIRCVKSNAILLAHDQATVGIGMGQVNRVDSCHLAVERANTLADGADRATGAVAASDAFFPFADGAQVLIDAG
VKAIVQPGGSIRDEEVIEAANKAGVTMYLTGTRHFFH
>Mature_517_residues
MKAGTEVVSTGSTARKLAELGVKVTEVSDVTGFPECLDGRVKTLHPYIHAGILADMTNPEHAKQLDGFGIKPFDLVVVNL
YPFADTVRSGANEADTIEKIDIGGPSMVRGAAKNHATVAIVTDPADYALVAERVADGTGFSLDERKWLAAKAFAHTAAYD
ATINEWTAKHWPKPASLDTAEVDKDDQDTEIDSAKFPARFTRTWDRAHTLRYGENSHQQAALYIDPLNQTGFAHAEQLGG
KPMSYNNYVDADAAWRTVWDMAPAIAVAVVKHNNPCGLAIGATVAEAHKKAHACDPMSAYGGVIACNSKVTLEMAESVRP
IFTEVIVAPDYEPAALELLQTKKKNLRILKVAEPPKGHEAIRQIDGGLLVQDTDLINAVGDDPDAWKLVAGEAADADTLK
DLVFAWRAIRCVKSNAILLAHDQATVGIGMGQVNRVDSCHLAVERANTLADGADRATGAVAASDAFFPFADGAQVLIDAG
VKAIVQPGGSIRDEEVIEAANKAGVTMYLTGTRHFFH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=604, Percent_Identity=29.8013245033113, Blast_Score=207, Evalue=2e-53,
Organism=Escherichia coli, GI1790439, Length=526, Percent_Identity=44.106463878327, Blast_Score=389, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI71985564, Length=486, Percent_Identity=32.9218106995885, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI71985574, Length=335, Percent_Identity=28.3582089552239, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323768, Length=476, Percent_Identity=32.1428571428571, Blast_Score=200, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6323056, Length=470, Percent_Identity=31.7021276595745, Blast_Score=195, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24649832, Length=472, Percent_Identity=32.2033898305085, Blast_Score=193, Evalue=2e-49,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55353; Mature: 55353

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAGTEVVSTGSTARKLAELGVKVTEVSDVTGFPECLDGRVKTLHPYIHAGILADMTNPE
CCCCCCHHCCCHHHHHHHHCCCEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCH
HAKQLDGFGIKPFDLVVVNLYPFADTVRSGANEADTIEKIDIGGPSMVRGAAKNHATVAI
HHHHCCCCCCCCEEEEEEEEECCHHHHHCCCCCCCCEEEEECCCHHHHHCCCCCCEEEEE
VTDPADYALVAERVADGTGFSLDERKWLAAKAFAHTAAYDATINEWTAKHWPKPASLDTA
EECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHH
EVDKDDQDTEIDSAKFPARFTRTWDRAHTLRYGENSHQQAALYIDPLNQTGFAHAEQLGG
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCEEEEEEECCCCCCCHHHHHHCC
KPMSYNNYVDADAAWRTVWDMAPAIAVAVVKHNNPCGLAIGATVAEAHKKAHACDPMSAY
CCCCCCCCCCCCHHHHHHHHHHHEEEEEEEEECCCCCEEEHHHHHHHHHHHCCCCCHHHC
GGVIACNSKVTLEMAESVRPIFTEVIVAPDYEPAALELLQTKKKNLRILKVAEPPKGHEA
CCEEEECCEEEEHHHHHHHHHHHHEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHH
IRQIDGGLLVQDTDLINAVGDDPDAWKLVAGEAADADTLKDLVFAWRAIRCVKSNAILLA
HHHHCCCEEEECCHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
HDQATVGIGMGQVNRVDSCHLAVERANTLADGADRATGAVAASDAFFPFADGAQVLIDAG
ECCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCHHEEHHC
VKAIVQPGGSIRDEEVIEAANKAGVTMYLTGTRHFFH
CHHEECCCCCCCHHHHHHHHCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MKAGTEVVSTGSTARKLAELGVKVTEVSDVTGFPECLDGRVKTLHPYIHAGILADMTNPE
CCCCCCHHCCCHHHHHHHHCCCEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCH
HAKQLDGFGIKPFDLVVVNLYPFADTVRSGANEADTIEKIDIGGPSMVRGAAKNHATVAI
HHHHCCCCCCCCEEEEEEEEECCHHHHHCCCCCCCCEEEEECCCHHHHHCCCCCCEEEEE
VTDPADYALVAERVADGTGFSLDERKWLAAKAFAHTAAYDATINEWTAKHWPKPASLDTA
EECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHH
EVDKDDQDTEIDSAKFPARFTRTWDRAHTLRYGENSHQQAALYIDPLNQTGFAHAEQLGG
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCEEEEEEECCCCCCCHHHHHHCC
KPMSYNNYVDADAAWRTVWDMAPAIAVAVVKHNNPCGLAIGATVAEAHKKAHACDPMSAY
CCCCCCCCCCCCHHHHHHHHHHHEEEEEEEEECCCCCEEEHHHHHHHHHHHCCCCCHHHC
GGVIACNSKVTLEMAESVRPIFTEVIVAPDYEPAALELLQTKKKNLRILKVAEPPKGHEA
CCEEEECCEEEEHHHHHHHHHHHHEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHH
IRQIDGGLLVQDTDLINAVGDDPDAWKLVAGEAADADTLKDLVFAWRAIRCVKSNAILLA
HHHHCCCEEEECCHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
HDQATVGIGMGQVNRVDSCHLAVERANTLADGADRATGAVAASDAFFPFADGAQVLIDAG
ECCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCHHEEHHC
VKAIVQPGGSIRDEEVIEAANKAGVTMYLTGTRHFFH
CHHEECCCCCCCHHHHHHHHCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA