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The map label for this gene is brnQ [H]

Identifier: 213691976

GI number: 213691976

Start: 1280779

End: 1282137

Strand: Reverse

Name: brnQ [H]

Synonym: Blon_1094

Alternate gene names: 213691976

Gene position: 1282137-1280779 (Counterclockwise)

Preceding gene: 213691977

Following gene: 213691958

Centisome position: 45.26

GC content: 62.77

Gene sequence:

>1359_bases
ATGAACAAGCTTGTCGCGCACCAACGGTTGGTGATGACATTTACCTTCTTTTCGATGTTCTTCGGGGCGGGCAACCTGAT
CTTCCCTCCATTCGTTGGCGCGCAGGCCGGATCGGCGACCATGCCGGCAGCCGTCGGCTTCATCGTCTCCGCGGTCGGAT
TGCCGATTCTCGGCGTCCTCGCGGTCACCTTCGCAGGGGGATTCGATGGGCTCGCCCGACGGGTCTCCCCCGGATTCGCC
CTGTTTCTTGGTGTGGCGATCATGCTTACCATCGGCCCCTGCTTTGCGATTCCGCGCACGGCCACCACGTCATTCGAGAT
GATGGTGTCGCCTTTCGTGCCGGCTTCCTACGGCTGGCTCGCGCAACTCATCTACTCGTGCGTGTTCTTCGCATTGGCCT
TCCTGCTCGCCCAGCATCCCGAGAAACTCTCCAAGGTGCTTGGACGTTTCATGGGACCGCTGCTGCTGGTGCTGATCGCC
ATCCTGTTCGTCGCATGCCTGGTACACGGCATCGGCACGCCGGCCAAACCGATGGGCGACTACTTCGCCAATCAGGTGAC
GCGAGGCTTCCTCGACGGCTACCAGACCATGGATCTGCTCGCCGCGCTGTACTTCGGCATCGTGATCTCCGCGAACATCC
GCGCCCAGCACATCGATGACGAGATCCAAGTGCAGAAGGAGACCGCATACGCCGGTCTTGGCACCGGCGTTCTGCTCATC
GTGATCTATGGAGCGCTGAGCTATGTGGGAGCGGTATCCGGCGCCATCAGCGCCATCGACCCGGCAAAGGACACGGGCGC
GACCGTGCTCACCAACCTCACCTCGTCCCTGTTCGGCACCTTCGGCACCGCGTTCCTCGGTATCGTGTTCGTCATAGCCT
GCCTCAACGTGTGCACCGGACTGATCTGCACCTGCGCCACCTATTTCCACGCCAGATTCCGCTCCATCGCGGGACGAACG
CCGAGCTACCGCACGTGGCAGGTCATCTTCACCGTGTTCAGCTTCATCGTCTCCAACGCCGGACTGAGCGCGATCATCAA
GGTCTCCGTGCCGGTACTGTCCGCATTGTATCCGATCGCCATCGTACTGGTGCTGCTCGCGCTCACCCATACCATGCTCG
GCGCGCGGTTCCCGCGCGTCTATTTCTGGACCGTGCTGCTGGTGGCCGTCACCTCCTTCGCCACCTGTGTCTCCAGCTTG
GCCGTCGTGTTCGGCGGCGACATCGCCTGGCTGGATGCCGCGCTGGCCGCACTGCCCTTGCAGCAGTACCAGCTCGGCTG
GATCCTACCCGCCGTCATCGGCGTCATTGCCGGCATCGTCGACAAGCGGCGCGTCACGCCTGCCGGCCGCGACATCTAG

Upstream 100 bases:

>100_bases
TCTGCGAGGGTGCCCCGGAAGGGAGACCCGTCATGCGCAGACCCGGGGACAATCCGATGCCATAGTGCTACGGCCACACG
GGAGAAAGGTGTTTTCCGCA

Downstream 100 bases:

>100_bases
ACCAGATTCCGTCGGATCCTGCGGTAGGTGATGAGGAACAGCAGACCGGCCACCATGCCCGAGGTGGCGTCGGAGATGGG
TTCGGCGATGAAGATGCCAT

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BrnQ [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MNKLVAHQRLVMTFTFFSMFFGAGNLIFPPFVGAQAGSATMPAAVGFIVSAVGLPILGVLAVTFAGGFDGLARRVSPGFA
LFLGVAIMLTIGPCFAIPRTATTSFEMMVSPFVPASYGWLAQLIYSCVFFALAFLLAQHPEKLSKVLGRFMGPLLLVLIA
ILFVACLVHGIGTPAKPMGDYFANQVTRGFLDGYQTMDLLAALYFGIVISANIRAQHIDDEIQVQKETAYAGLGTGVLLI
VIYGALSYVGAVSGAISAIDPAKDTGATVLTNLTSSLFGTFGTAFLGIVFVIACLNVCTGLICTCATYFHARFRSIAGRT
PSYRTWQVIFTVFSFIVSNAGLSAIIKVSVPVLSALYPIAIVLVLLALTHTMLGARFPRVYFWTVLLVAVTSFATCVSSL
AVVFGGDIAWLDAALAALPLQQYQLGWILPAVIGVIAGIVDKRRVTPAGRDI

Sequences:

>Translated_452_residues
MNKLVAHQRLVMTFTFFSMFFGAGNLIFPPFVGAQAGSATMPAAVGFIVSAVGLPILGVLAVTFAGGFDGLARRVSPGFA
LFLGVAIMLTIGPCFAIPRTATTSFEMMVSPFVPASYGWLAQLIYSCVFFALAFLLAQHPEKLSKVLGRFMGPLLLVLIA
ILFVACLVHGIGTPAKPMGDYFANQVTRGFLDGYQTMDLLAALYFGIVISANIRAQHIDDEIQVQKETAYAGLGTGVLLI
VIYGALSYVGAVSGAISAIDPAKDTGATVLTNLTSSLFGTFGTAFLGIVFVIACLNVCTGLICTCATYFHARFRSIAGRT
PSYRTWQVIFTVFSFIVSNAGLSAIIKVSVPVLSALYPIAIVLVLLALTHTMLGARFPRVYFWTVLLVAVTSFATCVSSL
AVVFGGDIAWLDAALAALPLQQYQLGWILPAVIGVIAGIVDKRRVTPAGRDI
>Mature_452_residues
MNKLVAHQRLVMTFTFFSMFFGAGNLIFPPFVGAQAGSATMPAAVGFIVSAVGLPILGVLAVTFAGGFDGLARRVSPGFA
LFLGVAIMLTIGPCFAIPRTATTSFEMMVSPFVPASYGWLAQLIYSCVFFALAFLLAQHPEKLSKVLGRFMGPLLLVLIA
ILFVACLVHGIGTPAKPMGDYFANQVTRGFLDGYQTMDLLAALYFGIVISANIRAQHIDDEIQVQKETAYAGLGTGVLLI
VIYGALSYVGAVSGAISAIDPAKDTGATVLTNLTSSLFGTFGTAFLGIVFVIACLNVCTGLICTCATYFHARFRSIAGRT
PSYRTWQVIFTVFSFIVSNAGLSAIIKVSVPVLSALYPIAIVLVLLALTHTMLGARFPRVYFWTVLLVAVTSFATCVSSL
AVVFGGDIAWLDAALAALPLQQYQLGWILPAVIGVIAGIVDKRRVTPAGRDI

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=460, Percent_Identity=35.4347826086957, Blast_Score=229, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 47947; Mature: 47947

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023181; 9308178; 9384377 [H]