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The map label for this gene is def2

Identifier: 213691939

GI number: 213691939

Start: 1238882

End: 1239370

Strand: Direct

Name: def2

Synonym: Blon_1055

Alternate gene names: 213691939

Gene position: 1238882-1239370 (Clockwise)

Preceding gene: 213691938

Following gene: 213691940

Centisome position: 43.73

GC content: 63.39

Gene sequence:

>489_bases
GTGGCCATCCGTGAGATTAGGGTCGTGCCCGACCCAGTCCTGCGCACCCCCTGCGATGAGATCAAGGAGATCACCCCGGC
CGTGCGCCGCCTGGTTGACGACCTGTTGGAAACCGTGGACGATCCGGGCCGAGCGGGCCTGTCCGCCAACCAGATCGGCG
TGAGCCTGAGGGCCTTCTCCTACAACATCGACGGCAAGGTCGGCTACGTTCTGAACCCCGTACTCGAGGAGAAGTCCGGC
GAGCAGTACGGCGATGAAGGTTGTCTGTCTGTGCCCGGCTTATGGTACAAGACCCGTCGTGCCGACTACGCGCGCGTGCG
TGGCATCGACCTGGACGGCAACGAGGTCGTGCTCGAGGGCTCCGGGCTGATGGGCCGTATGCTTCAGCACGAATGCGACC
ACCTCGACGGCCACGTGTATTTGGATCGCCTGGAAAAGGAAGAACGCCGCGAAGCCCTGCGCTACATGCGCAATCGTCAG
GCGAAGTAG

Upstream 100 bases:

>100_bases
AGGGCGAGACCGGCGATTCCGCCGACAATGCCGACAATGCCGCTGACACCGGCAGCAAGCTGGACCAGCCGGCTGACGAG
AAGAACGAGGAGTAACGATA

Downstream 100 bases:

>100_bases
TCCATAATCGGCTCCCGCTGGCGGGAGCCGTCGAACATAGTGAGACTGAGGGTGGGTCTGCAGGTACCGCCCGCAACTCC
CCTCAGTCGCCTACGGCGAC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MAIREIRVVPDPVLRTPCDEIKEITPAVRRLVDDLLETVDDPGRAGLSANQIGVSLRAFSYNIDGKVGYVLNPVLEEKSG
EQYGDEGCLSVPGLWYKTRRADYARVRGIDLDGNEVVLEGSGLMGRMLQHECDHLDGHVYLDRLEKEERREALRYMRNRQ
AK

Sequences:

>Translated_162_residues
MAIREIRVVPDPVLRTPCDEIKEITPAVRRLVDDLLETVDDPGRAGLSANQIGVSLRAFSYNIDGKVGYVLNPVLEEKSG
EQYGDEGCLSVPGLWYKTRRADYARVRGIDLDGNEVVLEGSGLMGRMLQHECDHLDGHVYLDRLEKEERREALRYMRNRQ
AK
>Mature_161_residues
AIREIRVVPDPVLRTPCDEIKEITPAVRRLVDDLLETVDDPGRAGLSANQIGVSLRAFSYNIDGKVGYVLNPVLEEKSGE
QYGDEGCLSVPGLWYKTRRADYARVRGIDLDGNEVVLEGSGLMGRMLQHECDHLDGHVYLDRLEKEERREALRYMRNRQA
K

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Escherichia coli, GI1789682, Length=148, Percent_Identity=35.8108108108108, Blast_Score=94, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24645726, Length=159, Percent_Identity=28.3018867924528, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24645728, Length=165, Percent_Identity=29.0909090909091, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF2_BIFLO (Q8G487)

Other databases:

- EMBL:   AE014295
- RefSeq:   NP_696661.1
- ProteinModelPortal:   Q8G487
- SMR:   Q8G487
- GeneID:   1021664
- GenomeReviews:   AE014295_GR
- KEGG:   blo:BL1502
- HOGENOM:   HBG665227
- OMA:   IHEIDDG
- ProtClustDB:   CLSK2304359
- BioCyc:   BLON206672:BL1502-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 18369; Mature: 18238

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIREIRVVPDPVLRTPCDEIKEITPAVRRLVDDLLETVDDPGRAGLSANQIGVSLRAFS
CCCCCEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCEEEEEEE
YNIDGKVGYVLNPVLEEKSGEQYGDEGCLSVPGLWYKTRRADYARVRGIDLDGNEVVLEG
ECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCHHHHCCCCCCCCEEEEEC
SGLMGRMLQHECDHLDGHVYLDRLEKEERREALRYMRNRQAK
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AIREIRVVPDPVLRTPCDEIKEITPAVRRLVDDLLETVDDPGRAGLSANQIGVSLRAFS
CCCCEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCEEEEEEE
YNIDGKVGYVLNPVLEEKSGEQYGDEGCLSVPGLWYKTRRADYARVRGIDLDGNEVVLEG
ECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCHHHHCCCCCCCCEEEEEC
SGLMGRMLQHECDHLDGHVYLDRLEKEERREALRYMRNRQAK
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12381787