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The map label for this gene is spaK [H]

Identifier: 213691892

GI number: 213691892

Start: 1188453

End: 1189817

Strand: Direct

Name: spaK [H]

Synonym: Blon_1004

Alternate gene names: 213691892

Gene position: 1188453-1189817 (Clockwise)

Preceding gene: 213691891

Following gene: 213691895

Centisome position: 41.95

GC content: 46.01

Gene sequence:

>1365_bases
ATGGGAACAAAGATGAAGACGACGCTTACAAGAACTTTTTATAGATTTCTTGTATGTCTGCTTTGCGGACTGTGTATGTC
CGTAGTCATCCCCTTTGCCTGCATGATGTTCGCCTCCAGCATGGGGCTTGTAACGTTTGCGGATAATGCCGAACGGCAAG
CGAAAAATCTGGTGCCGATACTCACCGCAACGCCAGATGTATCAGACGTGTATCTGCCCATTGGGATTAAATACCTTCGG
TTGGATAGAAACTATGGATCCATAGATACGAATATGAACGAAGAAGAGAAAGAGAAGGCTCTGGCGTTTGCCGAGACGGG
TCTCATAGATGCAGGCGGTAGAACACAGTTCATCTTCATCACTCGAGATGATGGGTACATTGTTCTGCAGTACGTCATAG
GTTCTCAGTATCTCAATGCACAGTGCAACAAGTACCTACCGTCTCCGGAGATACTGCTGATTGCTTTGATCATCGCTAAC
AGCTTAGGCTTATGCGTCTACTTGACGGTTCATTTCGCCAGAGAGCTGAGAAAAGAATTGACGCCTATATTAGAAGCCAC
TAAAGAGATTGAAAATCACAACTTAGATTTTGCTATCAGGCATTCTCGAATACTTGAATTTGAAAACGTGGTTTCATCTT
TTGATAGTATGCGAAACAGTTTGAAAGAGTCATTGGAACAGCAGTGGCGAAGTGAACAAGCGCAGAGAGAGCAGATCGCA
TCCTTGGCCCATGATCTGAAGACTCCATTGACTGTTATGCAGGGGAACATCGACCTGTTGGATGAGACGACGCTTGACGA
AGAACAAAAGCTCTATCTGTCGTATGCGATGAGAAGCTCTGAGCAGATGAGACGGTACATAAAGATGATGATTGATATAT
CCCGAACATCCTGCGGATATCAATTGCAGATGGAAGACATATGCTTCCCGGAGTTCTGGAGACATGTCATATCTCAAGCT
GAAATCATCTGTAGAGATAAAGGTATTGTGATTCAGCAGACGCAGCACGCTCTTCCGAAACATATAACTGGGGATGGAGT
ATTGTTGGAGCGGGCATTGATGAATGTGGTTTCAAATGCCGTAGAGCACTCGCCCGTAGACTCTCCGCTATATATGGATG
CGGATGGCCAGGGTGATCACTTGAATGTCTGCATTACGGACTGTGGCTGTGGTTTTTCGCAAGAGGCATTGAAGCACGCG
AAAGAGCGTTTCTATATGGCAGACCAGAGCAGAGGCTCCGAACTGCATTATGGGATGGGCCTATATATCGTTGACGTCAT
CATAAAACAGCATAGAGGAGAACTGCTCCTCAGTAATTCCGCAGAAACCAAAGGCGCCAGGGTCACGATACGGCTACCAC
GATAA

Upstream 100 bases:

>100_bases
GCGATGGTTCTACAATTTCGACGCATATAAAAAACATAAGGTCGAAGTTCGAGGAGGCCGGTGTTCAACCAATCGTAACG
GTCTGGGGGATAGGCTATAA

Downstream 100 bases:

>100_bases
GGGAACGACTCATGCGTCGTTCCCTTATCATATCTCCAAGCGATATTCGGCATGGTGGTCCCGGATTCAACTTAGCCGTC
GACTGACGTCGCGGCCGTTG

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Subtilin biosynthesis sensor protein spaK [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MGTKMKTTLTRTFYRFLVCLLCGLCMSVVIPFACMMFASSMGLVTFADNAERQAKNLVPILTATPDVSDVYLPIGIKYLR
LDRNYGSIDTNMNEEEKEKALAFAETGLIDAGGRTQFIFITRDDGYIVLQYVIGSQYLNAQCNKYLPSPEILLIALIIAN
SLGLCVYLTVHFARELRKELTPILEATKEIENHNLDFAIRHSRILEFENVVSSFDSMRNSLKESLEQQWRSEQAQREQIA
SLAHDLKTPLTVMQGNIDLLDETTLDEEQKLYLSYAMRSSEQMRRYIKMMIDISRTSCGYQLQMEDICFPEFWRHVISQA
EIICRDKGIVIQQTQHALPKHITGDGVLLERALMNVVSNAVEHSPVDSPLYMDADGQGDHLNVCITDCGCGFSQEALKHA
KERFYMADQSRGSELHYGMGLYIVDVIIKQHRGELLLSNSAETKGARVTIRLPR

Sequences:

>Translated_454_residues
MGTKMKTTLTRTFYRFLVCLLCGLCMSVVIPFACMMFASSMGLVTFADNAERQAKNLVPILTATPDVSDVYLPIGIKYLR
LDRNYGSIDTNMNEEEKEKALAFAETGLIDAGGRTQFIFITRDDGYIVLQYVIGSQYLNAQCNKYLPSPEILLIALIIAN
SLGLCVYLTVHFARELRKELTPILEATKEIENHNLDFAIRHSRILEFENVVSSFDSMRNSLKESLEQQWRSEQAQREQIA
SLAHDLKTPLTVMQGNIDLLDETTLDEEQKLYLSYAMRSSEQMRRYIKMMIDISRTSCGYQLQMEDICFPEFWRHVISQA
EIICRDKGIVIQQTQHALPKHITGDGVLLERALMNVVSNAVEHSPVDSPLYMDADGQGDHLNVCITDCGCGFSQEALKHA
KERFYMADQSRGSELHYGMGLYIVDVIIKQHRGELLLSNSAETKGARVTIRLPR
>Mature_453_residues
GTKMKTTLTRTFYRFLVCLLCGLCMSVVIPFACMMFASSMGLVTFADNAERQAKNLVPILTATPDVSDVYLPIGIKYLRL
DRNYGSIDTNMNEEEKEKALAFAETGLIDAGGRTQFIFITRDDGYIVLQYVIGSQYLNAQCNKYLPSPEILLIALIIANS
LGLCVYLTVHFARELRKELTPILEATKEIENHNLDFAIRHSRILEFENVVSSFDSMRNSLKESLEQQWRSEQAQREQIAS
LAHDLKTPLTVMQGNIDLLDETTLDEEQKLYLSYAMRSSEQMRRYIKMMIDISRTSCGYQLQMEDICFPEFWRHVISQAE
IICRDKGIVIQQTQHALPKHITGDGVLLERALMNVVSNAVEHSPVDSPLYMDADGQGDHLNVCITDCGCGFSQEALKHAK
ERFYMADQSRGSELHYGMGLYIVDVIIKQHRGELLLSNSAETKGARVTIRLPR

Specific function: Member of the two-component regulatory system spaK/spaR involved in the regulation of the biosynthesis of lantibiotic subtilin. SpaK may function as a membrane-associated protein kinase that phosphorylates spaR in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=225, Percent_Identity=26.2222222222222, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790551, Length=290, Percent_Identity=25.8620689655172, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790346, Length=251, Percent_Identity=27.0916334661355, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786783, Length=339, Percent_Identity=24.4837758112094, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790861, Length=382, Percent_Identity=21.9895287958115, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI145693157, Length=276, Percent_Identity=25.7246376811594, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1786912, Length=275, Percent_Identity=27.6363636363636, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788713, Length=297, Percent_Identity=23.9057239057239, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787894, Length=344, Percent_Identity=24.4186046511628, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI48994928, Length=226, Percent_Identity=25.2212389380531, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788549, Length=234, Percent_Identity=26.0683760683761, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789149, Length=238, Percent_Identity=26.890756302521, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR008358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51484; Mature: 51353

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTKMKTTLTRTFYRFLVCLLCGLCMSVVIPFACMMFASSMGLVTFADNAERQAKNLVPI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCEE
LTATPDVSDVYLPIGIKYLRLDRNYGSIDTNMNEEEKEKALAFAETGLIDAGGRTQFIFI
EECCCCCCCEEEECCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEECCCCEEEEEE
TRDDGYIVLQYVIGSQYLNAQCNKYLPSPEILLIALIIANSLGLCVYLTVHFARELRKEL
EECCCEEEEEEECCCHHHCCHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
TPILEATKEIENHNLDFAIRHSRILEFENVVSSFDSMRNSLKESLEQQWRSEQAQREQIA
HHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLAHDLKTPLTVMQGNIDLLDETTLDEEQKLYLSYAMRSSEQMRRYIKMMIDISRTSCGY
HHHHHHCCCCEEECCCCEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCC
QLQMEDICFPEFWRHVISQAEIICRDKGIVIQQTQHALPKHITGDGVLLERALMNVVSNA
EEEHHCCCCHHHHHHHHHHHHEEECCCCEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHH
VEHSPVDSPLYMDADGQGDHLNVCITDCGCGFSQEALKHAKERFYMADQSRGSELHYGMG
HHCCCCCCCEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHEECCCCCCCCEECCHH
LYIVDVIIKQHRGELLLSNSAETKGARVTIRLPR
HHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCC
>Mature Secondary Structure 
GTKMKTTLTRTFYRFLVCLLCGLCMSVVIPFACMMFASSMGLVTFADNAERQAKNLVPI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCEE
LTATPDVSDVYLPIGIKYLRLDRNYGSIDTNMNEEEKEKALAFAETGLIDAGGRTQFIFI
EECCCCCCCEEEECCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEECCCCEEEEEE
TRDDGYIVLQYVIGSQYLNAQCNKYLPSPEILLIALIIANSLGLCVYLTVHFARELRKEL
EECCCEEEEEEECCCHHHCCHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
TPILEATKEIENHNLDFAIRHSRILEFENVVSSFDSMRNSLKESLEQQWRSEQAQREQIA
HHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLAHDLKTPLTVMQGNIDLLDETTLDEEQKLYLSYAMRSSEQMRRYIKMMIDISRTSCGY
HHHHHHCCCCEEECCCCEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCC
QLQMEDICFPEFWRHVISQAEIICRDKGIVIQQTQHALPKHITGDGVLLERALMNVVSNA
EEEHHCCCCHHHHHHHHHHHHEEECCCCEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHH
VEHSPVDSPLYMDADGQGDHLNVCITDCGCGFSQEALKHAKERFYMADQSRGSELHYGMG
HHCCCCCCCEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHEECCCCCCCCEECCHH
LYIVDVIIKQHRGELLLSNSAETKGARVTIRLPR
HHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8439156; 8085823 [H]