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The map label for this gene is ygcA [C]

Identifier: 213691821

GI number: 213691821

Start: 1096483

End: 1097751

Strand: Direct

Name: ygcA [C]

Synonym: Blon_0929

Alternate gene names: 213691821

Gene position: 1096483-1097751 (Clockwise)

Preceding gene: 213691820

Following gene: 213691822

Centisome position: 38.71

GC content: 63.99

Gene sequence:

>1269_bases
ATGCAAGCTGAAGTTCGTATCGAACGATACGCCGACCAAGGCCGCTGCGTGGCCCATATCGACGGTCGTGTGGTGTTCGT
CCGCTTCGCCTTGCCCGACGAACTCGTACGAGTCGAGCTTGACGAGCCCCACGACCGGGACGACCGGTTCTGGACCGGCG
AAGTCGTCGAGGTTCTCGAGCCGTCTGAAGACCGAGTCACCCCGGCATGGCCACTTGCCGGTCCCTTGGCGATGGGCGGG
GGAGTGGGCGGCGCGGATCTCGTGCACGTCTCTTTGCCCGGCCAGCTCAAGTGGAAGGCCATCACCGTCTCCGAACAGAT
GAGCCGACTCGGCCATATCGACGTGGCCGTTCCCATCGAGCGCATGCCCGGCGACAAGGAAGCCGGCGGCCTCAATTGGC
GTACCCGCATCGAAATGATTGCCGACGACAACGGCATGCCATCCATGCGCCGGCGCGGCACACACAACCGCGTGGCCATC
GACACCATGCCGCTTGCCACGCGCACGCTGCTGGACGTCGCCAAGCGCGAACACGTGTGGGAGGGCGGCTTTGAACCCGG
CTCCCAGATTCGTCTTTCCGTGCCCGAACCGCGCGGCGAGATCGTCGATACCGCGGCAGCAGACGACAATTACGCCGTAC
TTGTGGACGGCGAACTGCGCGCCGGCTCCCAGCTGCTCACCGAACAGGTGACTATCAACGGCACGACCTTCGACTATCAG
GTTGACGTCAACGGTTTCTGGCAAGTGCATCGCCAAGCGCCGATCGCCCTCGGTACGCACGTGATCAACCTGGTCAACGG
GCAGCTGCAGTCAGCGGCGGACGCCGTAATCTGGGATCTGTATTCCGGTTCCGGCCTGTTCACCCTGCCGCTGGCCACAA
TGACCGGTGAACGCACCCGCATGCTCAGCGTTGAAGGCGCGCGTGTGGCCGTCAAGAACGCTCAGCGCAACCTGCGCGCC
ATGAACCTGAACGATGTGGACGCCCGCGCGGGTGACGTTTCGCGCACGCTGGACCATGTGCCGGCGCATCTGGCCAAACC
GAATGTCGTAGTGCTTGATCCGCCGCGTGCCGGTGCTCGTGCCAAGGTCTGCCGCCAGATTGCCGCGGCCGGCGCATCCA
GCGTGGTCTACATCGCCTGCGATCCCACCAGCCTCGCACGCGACACCGCGATACTCATCGGCGAAGGCTACGAACTCAAA
GACATCCGTGCCTTTGACATCTACCCGATGACCCACCACGTCGAGACGGTGGCACTGTTTACCCGCTGA

Upstream 100 bases:

>100_bases
AAGGTCGCGGCTAAGAAGATACAGACTGAAGTCGCTGTTGATGCCGAATCCGAGGCAAACAATGCCGAACTCGAACCGAG
CGCAACCGGAAAGGACGATG

Downstream 100 bases:

>100_bases
CAGGTGAGCATATGACACGACGAATTCTCGGATTGCTGGCCTGCATGGCGGCTGTGTTGTCCCTAGCTGCCTGCACGCCG
GCCGGTAGGGCGGTCGGCGA

Product: (Uracil-5)-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MQAEVRIERYADQGRCVAHIDGRVVFVRFALPDELVRVELDEPHDRDDRFWTGEVVEVLEPSEDRVTPAWPLAGPLAMGG
GVGGADLVHVSLPGQLKWKAITVSEQMSRLGHIDVAVPIERMPGDKEAGGLNWRTRIEMIADDNGMPSMRRRGTHNRVAI
DTMPLATRTLLDVAKREHVWEGGFEPGSQIRLSVPEPRGEIVDTAAADDNYAVLVDGELRAGSQLLTEQVTINGTTFDYQ
VDVNGFWQVHRQAPIALGTHVINLVNGQLQSAADAVIWDLYSGSGLFTLPLATMTGERTRMLSVEGARVAVKNAQRNLRA
MNLNDVDARAGDVSRTLDHVPAHLAKPNVVVLDPPRAGARAKVCRQIAAAGASSVVYIACDPTSLARDTAILIGEGYELK
DIRAFDIYPMTHHVETVALFTR

Sequences:

>Translated_422_residues
MQAEVRIERYADQGRCVAHIDGRVVFVRFALPDELVRVELDEPHDRDDRFWTGEVVEVLEPSEDRVTPAWPLAGPLAMGG
GVGGADLVHVSLPGQLKWKAITVSEQMSRLGHIDVAVPIERMPGDKEAGGLNWRTRIEMIADDNGMPSMRRRGTHNRVAI
DTMPLATRTLLDVAKREHVWEGGFEPGSQIRLSVPEPRGEIVDTAAADDNYAVLVDGELRAGSQLLTEQVTINGTTFDYQ
VDVNGFWQVHRQAPIALGTHVINLVNGQLQSAADAVIWDLYSGSGLFTLPLATMTGERTRMLSVEGARVAVKNAQRNLRA
MNLNDVDARAGDVSRTLDHVPAHLAKPNVVVLDPPRAGARAKVCRQIAAAGASSVVYIACDPTSLARDTAILIGEGYELK
DIRAFDIYPMTHHVETVALFTR
>Mature_422_residues
MQAEVRIERYADQGRCVAHIDGRVVFVRFALPDELVRVELDEPHDRDDRFWTGEVVEVLEPSEDRVTPAWPLAGPLAMGG
GVGGADLVHVSLPGQLKWKAITVSEQMSRLGHIDVAVPIERMPGDKEAGGLNWRTRIEMIADDNGMPSMRRRGTHNRVAI
DTMPLATRTLLDVAKREHVWEGGFEPGSQIRLSVPEPRGEIVDTAAADDNYAVLVDGELRAGSQLLTEQVTINGTTFDYQ
VDVNGFWQVHRQAPIALGTHVINLVNGQLQSAADAVIWDLYSGSGLFTLPLATMTGERTRMLSVEGARVAVKNAQRNLRA
MNLNDVDARAGDVSRTLDHVPAHLAKPNVVVLDPPRAGARAKVCRQIAAAGASSVVYIACDPTSLARDTAILIGEGYELK
DIRAFDIYPMTHHVETVALFTR

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789148, Length=439, Percent_Identity=25.7403189066059, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 46175; Mature: 46175

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAEVRIERYADQGRCVAHIDGRVVFVRFALPDELVRVELDEPHDRDDRFWTGEVVEVLE
CCCEEEEEECCCCCCEEEEECCEEEEEEEECCCEEEEEECCCCCCCCCCEECCCHHEEEC
PSEDRVTPAWPLAGPLAMGGGVGGADLVHVSLPGQLKWKAITVSEQMSRLGHIDVAVPIE
CCCCCCCCCCCCCCCHHCCCCCCCCEEEEEECCCCEEEEEEEHHHHHHHCCCEEEEEEHH
RMPGDKEAGGLNWRTRIEMIADDNGMPSMRRRGTHNRVAIDTMPLATRTLLDVAKREHVW
HCCCCCCCCCCCEEEEEEEEECCCCCCHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHC
EGGFEPGSQIRLSVPEPRGEIVDTAAADDNYAVLVDGELRAGSQLLTEQVTINGTTFDYQ
CCCCCCCCEEEEECCCCCCCEEEEECCCCCEEEEECCCCCCCCHHEEEEEEECCEEEEEE
VDVNGFWQVHRQAPIALGTHVINLVNGQLQSAADAVIWDLYSGSGLFTLPLATMTGERTR
EECCCEEEEECCCCCHHHHHHHHHHCCHHHHCCCEEEEEEECCCCEEEEEEEEECCCCEE
MLSVEGARVAVKNAQRNLRAMNLNDVDARAGDVSRTLDHVPAHLAKPNVVVLDPPRAGAR
EEEECCCEEEEECHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHH
AKVCRQIAAAGASSVVYIACDPTSLARDTAILIGEGYELKDIRAFDIYPMTHHVETVALF
HHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCEEEEEEEECHHCEEEEEEE
TR
EC
>Mature Secondary Structure
MQAEVRIERYADQGRCVAHIDGRVVFVRFALPDELVRVELDEPHDRDDRFWTGEVVEVLE
CCCEEEEEECCCCCCEEEEECCEEEEEEEECCCEEEEEECCCCCCCCCCEECCCHHEEEC
PSEDRVTPAWPLAGPLAMGGGVGGADLVHVSLPGQLKWKAITVSEQMSRLGHIDVAVPIE
CCCCCCCCCCCCCCCHHCCCCCCCCEEEEEECCCCEEEEEEEHHHHHHHCCCEEEEEEHH
RMPGDKEAGGLNWRTRIEMIADDNGMPSMRRRGTHNRVAIDTMPLATRTLLDVAKREHVW
HCCCCCCCCCCCEEEEEEEEECCCCCCHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHC
EGGFEPGSQIRLSVPEPRGEIVDTAAADDNYAVLVDGELRAGSQLLTEQVTINGTTFDYQ
CCCCCCCCEEEEECCCCCCCEEEEECCCCCEEEEECCCCCCCCHHEEEEEEECCEEEEEE
VDVNGFWQVHRQAPIALGTHVINLVNGQLQSAADAVIWDLYSGSGLFTLPLATMTGERTR
EECCCEEEEECCCCCHHHHHHHHHHCCHHHHCCCEEEEEEECCCCEEEEEEEEECCCCEE
MLSVEGARVAVKNAQRNLRAMNLNDVDARAGDVSRTLDHVPAHLAKPNVVVLDPPRAGAR
EEEECCCEEEEECHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHH
AKVCRQIAAAGASSVVYIACDPTSLARDTAILIGEGYELKDIRAFDIYPMTHHVETVALF
HHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCEEEEEEEECHHCEEEEEEE
TR
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]