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The map label for this gene is mraW [H]

Identifier: 213691742

GI number: 213691742

Start: 999855

End: 1000934

Strand: Direct

Name: mraW [H]

Synonym: Blon_0848

Alternate gene names: 213691742

Gene position: 999855-1000934 (Clockwise)

Preceding gene: 213691741

Following gene: 213691743

Centisome position: 35.3

GC content: 58.7

Gene sequence:

>1080_bases
ATGGTTGATGTGGCGAATATTCATCTGCCGGTATTGCTTGACGATTGCGTGAACCTGATGGCGCCTGCCCTGGAACATGA
GAACGCCGTTGCAGTGGATTGCACTTTGGGCCTTGCCGGCCATTCCATCGCCTTCCTCAAAACCGCGCCTCAGGCTCGGC
TCATCGGCATTGACCGTGACAGCGAAGCGCTGGGGCTGGCCGCCGAACGCATGGAACGGGAGGGGCTCGCCGATCGATTC
ATCCCAGTGCATGCCGCCTTCGATCAGCTTGATCAGGTGTTGACGGACCGAGGCATAGAACGTGTGGACGCCGTATTCAT
GGATCTGGGATTATCCAGCCTGCAGATCGACGAAACCGATCGTGGCTTCTCCTATTCGCATGATGCGCCGCTGGATATGC
GCATGGACGTCAGCCAGCCGTTGACGGCCGAACGGATTCTTGCCACCTATGATGCGGCGGAACTGGTCCGCATATTCAAG
GAATACGGTGAAGAGCGTTTCTCGCGTCAGATCGCCCGCGCCATCGTGGCGCATCGAGACAAGGAGCCGTTTACCACCAC
CGCACAGCTCAACCGTCTGGTCGATGAAGTGGTGCCGCAAGCCCACCGCCCGGCGGGCAATCCCGCCAAACGCGTCTTCC
AGGCGTTGCGCATTGAAGTCAACGGCGAATTAGACAAGCTCGCCTCCACATTGCCGCAAGCCGCGAACCGACTGCATGTC
GGGGGACGACTGGTGGTGGAGTCCTATCATTCGCTCGAAGACAAAACCGTCAAATCGTTCATGGCACAAGGGCTGCGAGT
GGATGTTCCGGCTGGCCTGCCCGTCATACCGCCGGATGCCCAACCGTTTTTCACGGATCTCACGCGAGGTGCCATCAAGG
CCGACGAACATGAAATCGCAGCCAACCCGAGATCGGCTTCGGTGCGGTTGCGGGCGGTGGAAGTCAGCCGTGATATTCCG
TCACGATGGAGGAAACGCTTTACGCAAACCGCGCAGGGCCTGAATGACATCAATATACAAGGCTCCGCGTCTCCGGGCAG
GGCCAAAAACACAGCACGAATTCGTACAAGGAGGGGATAA

Upstream 100 bases:

>100_bases
CCGAACTGTGGAATAAGGACACGTGGGAGTCCTACCTCGCCGAGAAGGAAGAGGGATATTCCGACATTGCTGACGATGTG
TTGCCGGAGGTGGAATTCTG

Downstream 100 bases:

>100_bases
CAGCATATGGCAACCGCACGTAACATTCGATCGAACGCGGCTCCGGCCTCGAACCGGCCGCACACCAAGCATCAGTCCTC
CTCCCGACCTGAGCTGCGGG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MVDVANIHLPVLLDDCVNLMAPALEHENAVAVDCTLGLAGHSIAFLKTAPQARLIGIDRDSEALGLAAERMEREGLADRF
IPVHAAFDQLDQVLTDRGIERVDAVFMDLGLSSLQIDETDRGFSYSHDAPLDMRMDVSQPLTAERILATYDAAELVRIFK
EYGEERFSRQIARAIVAHRDKEPFTTTAQLNRLVDEVVPQAHRPAGNPAKRVFQALRIEVNGELDKLASTLPQAANRLHV
GGRLVVESYHSLEDKTVKSFMAQGLRVDVPAGLPVIPPDAQPFFTDLTRGAIKADEHEIAANPRSASVRLRAVEVSRDIP
SRWRKRFTQTAQGLNDINIQGSASPGRAKNTARIRTRRG

Sequences:

>Translated_359_residues
MVDVANIHLPVLLDDCVNLMAPALEHENAVAVDCTLGLAGHSIAFLKTAPQARLIGIDRDSEALGLAAERMEREGLADRF
IPVHAAFDQLDQVLTDRGIERVDAVFMDLGLSSLQIDETDRGFSYSHDAPLDMRMDVSQPLTAERILATYDAAELVRIFK
EYGEERFSRQIARAIVAHRDKEPFTTTAQLNRLVDEVVPQAHRPAGNPAKRVFQALRIEVNGELDKLASTLPQAANRLHV
GGRLVVESYHSLEDKTVKSFMAQGLRVDVPAGLPVIPPDAQPFFTDLTRGAIKADEHEIAANPRSASVRLRAVEVSRDIP
SRWRKRFTQTAQGLNDINIQGSASPGRAKNTARIRTRRG
>Mature_359_residues
MVDVANIHLPVLLDDCVNLMAPALEHENAVAVDCTLGLAGHSIAFLKTAPQARLIGIDRDSEALGLAAERMEREGLADRF
IPVHAAFDQLDQVLTDRGIERVDAVFMDLGLSSLQIDETDRGFSYSHDAPLDMRMDVSQPLTAERILATYDAAELVRIFK
EYGEERFSRQIARAIVAHRDKEPFTTTAQLNRLVDEVVPQAHRPAGNPAKRVFQALRIEVNGELDKLASTLPQAANRLHV
GGRLVVESYHSLEDKTVKSFMAQGLRVDVPAGLPVIPPDAQPFFTDLTRGAIKADEHEIAANPRSASVRLRAVEVSRDIP
SRWRKRFTQTAQGLNDINIQGSASPGRAKNTARIRTRRG

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=272, Percent_Identity=36.7647058823529, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI165377202, Length=199, Percent_Identity=35.678391959799, Blast_Score=111, Evalue=8e-25,
Organism=Escherichia coli, GI1786270, Length=313, Percent_Identity=38.6581469648562, Blast_Score=194, Evalue=7e-51,
Organism=Drosophila melanogaster, GI62472493, Length=329, Percent_Identity=34.9544072948328, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI28571637, Length=329, Percent_Identity=34.9544072948328, Blast_Score=170, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 39612; Mature: 39612

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDVANIHLPVLLDDCVNLMAPALEHENAVAVDCTLGLAGHSIAFLKTAPQARLIGIDRD
CCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEEECCCCCEEEECCCC
SEALGLAAERMEREGLADRFIPVHAAFDQLDQVLTDRGIERVDAVFMDLGLSSLQIDETD
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEECCCC
RGFSYSHDAPLDMRMDVSQPLTAERILATYDAAELVRIFKEYGEERFSRQIARAIVAHRD
CCCCCCCCCCCCEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KEPFTTTAQLNRLVDEVVPQAHRPAGNPAKRVFQALRIEVNGELDKLASTLPQAANRLHV
CCCCHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEC
GGRLVVESYHSLEDKTVKSFMAQGLRVDVPAGLPVIPPDAQPFFTDLTRGAIKADEHEIA
CCCCEEHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHC
ANPRSASVRLRAVEVSRDIPSRWRKRFTQTAQGLNDINIQGSASPGRAKNTARIRTRRG
CCCCCCEEEEEEEEHHCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCHHEECCCC
>Mature Secondary Structure
MVDVANIHLPVLLDDCVNLMAPALEHENAVAVDCTLGLAGHSIAFLKTAPQARLIGIDRD
CCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEEECCCCCEEEECCCC
SEALGLAAERMEREGLADRFIPVHAAFDQLDQVLTDRGIERVDAVFMDLGLSSLQIDETD
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEECCCC
RGFSYSHDAPLDMRMDVSQPLTAERILATYDAAELVRIFKEYGEERFSRQIARAIVAHRD
CCCCCCCCCCCCEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KEPFTTTAQLNRLVDEVVPQAHRPAGNPAKRVFQALRIEVNGELDKLASTLPQAANRLHV
CCCCHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEC
GGRLVVESYHSLEDKTVKSFMAQGLRVDVPAGLPVIPPDAQPFFTDLTRGAIKADEHEIA
CCCCEEHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHC
ANPRSASVRLRAVEVSRDIPSRWRKRFTQTAQGLNDINIQGSASPGRAKNTARIRTRRG
CCCCCCEEEEEEEEHHCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA