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The map label for this gene is lacY [H]

Identifier: 213691683

GI number: 213691683

Start: 914204

End: 915529

Strand: Reverse

Name: lacY [H]

Synonym: Blon_0788

Alternate gene names: 213691683

Gene position: 915529-914204 (Counterclockwise)

Preceding gene: 213691684

Following gene: 213691682

Centisome position: 32.32

GC content: 58.22

Gene sequence:

>1326_bases
ATGGCAAGTGCAACCAAATCTGCATGGAAGAATCCTTCCTATCTGCAGAGCTCCTTCGGCATCTTCATGTTCTTCTGCTC
CTGGGGCATCTGGTGGTCCTTCTTCTCCCGCTGGCTCACCGACCCGACCCACGGTCTGGGCATGACCTCCGCAGAGCAGG
GCCAGATCTACTCCATTAACTCCTTGGCAACCCTGGTCATCATGTTCGCCTATGGTGCCATCCAAGATCAGCTGGGCATC
AAGCGCAAGCTCGTGATCTTCGTCTCCGCCATCGCAGCGCTCGTCGGCCCGTTCGTACAGTTCGTGTATGCCCCGATGCT
GACTGCCGGCGGCACCACCCGTTTCATCGGCGTGCTCATCGGCTCCATCGTGCTCTCCTCAGGCTTCATGGCCGGCTGTT
CCCTGTTCGAAGCGCTCACCGAACGCTACTCCCGTAAGTTCGGCTTCGAATACGGCCAGTCCCGCGCTTGGGGCTCCTTC
GGCTACGCCATCGTGGCACTGTGCGCAGGTTTCCTGTTCAACATCAACCCGCTGCTGAACTTCTGGGTCGGCTCCATCTG
CGGCCTTGGCATGCTGTGCGTCTACGCTTTCTGGGTTCCGGCCAAGCAAAAGGAAGAGCTCAAGAAAGAAGCCGATCCGA
ACGCGGCTCCAACCAACCCATCCTTCAAAGAGATGATCTCCGTTCTGAAGATGCCGACTCTGTGGGTGCTCATCGTCTTC
ATGCTGTTCACCAACACCTTCTACACCGTGTTCGACCAGCAGATGTTCCCTAACTACTACGCTTCCCTCTTCCCGACCAC
CGAAATCGGCAACGCCACCTACGGCACCCTGAACTCCTTCCAGGTGTTCCTCGAGTCCGCCATGATGGGCGTCGTTCCGA
TTATCATGAAGAAGATCGGCGTTCGCAACTCCCTGCTGCTCGGCGCAACCGTGATGTTCCTGCGTATCGGCCTGTGCGGC
GTGTTCCACGATCCGGTCAGTGTCTCCATCGTCAAGCTGTTCCACTCCATCGAGGTTCCGCTGTTCTGCCTACCGGCATT
CCGCTACTTCACCCTGCACTTCGACACCAAACTGTCGGCAACCCTGTACATGGTGGGCTTCCAGATCGCTTCCCAAATCG
GCCAGGTGATCTTCTCCACCCCGATGGGCGCCCTGCATGACGCCATGGGCGACCGTCCGACCTTCTTCACCATCTCCGGC
ATCGTGCTGGCCGCCCTAATCTACGGCTTCTTCGTGATCAAGAAGGACGACCAGGAAGTCGGCGGTGATCCGTTCTACAC
CGATAAGCAGCTCAAGGCCCAAGCCGCAGCTAAAGCAAACGCCTGA

Upstream 100 bases:

>100_bases
GCATAGTAATTACTACACAACAACTTCTACAAAGGCACAGCCGCCCGAGCAGAAGCGTCACATAACCAATAACCAACCAA
GTAGTAATCAAAGGATGATT

Downstream 100 bases:

>100_bases
GCGCAACCGCCAAAATCGGCAAGAACACAACTAAATCGTGCTGCCCGAAGCATTTCCCCTTTTTCCGTTTCGGGCAGCAT
TCTTAAAACTTTTATCTAAA

Product: galactoside permease

Products: Proton [Cytoplasm]; lactose [Cytoplasm] [C]

Alternate protein names: Sucrose permease [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MASATKSAWKNPSYLQSSFGIFMFFCSWGIWWSFFSRWLTDPTHGLGMTSAEQGQIYSINSLATLVIMFAYGAIQDQLGI
KRKLVIFVSAIAALVGPFVQFVYAPMLTAGGTTRFIGVLIGSIVLSSGFMAGCSLFEALTERYSRKFGFEYGQSRAWGSF
GYAIVALCAGFLFNINPLLNFWVGSICGLGMLCVYAFWVPAKQKEELKKEADPNAAPTNPSFKEMISVLKMPTLWVLIVF
MLFTNTFYTVFDQQMFPNYYASLFPTTEIGNATYGTLNSFQVFLESAMMGVVPIIMKKIGVRNSLLLGATVMFLRIGLCG
VFHDPVSVSIVKLFHSIEVPLFCLPAFRYFTLHFDTKLSATLYMVGFQIASQIGQVIFSTPMGALHDAMGDRPTFFTISG
IVLAALIYGFFVIKKDDQEVGGDPFYTDKQLKAQAAAKANA

Sequences:

>Translated_441_residues
MASATKSAWKNPSYLQSSFGIFMFFCSWGIWWSFFSRWLTDPTHGLGMTSAEQGQIYSINSLATLVIMFAYGAIQDQLGI
KRKLVIFVSAIAALVGPFVQFVYAPMLTAGGTTRFIGVLIGSIVLSSGFMAGCSLFEALTERYSRKFGFEYGQSRAWGSF
GYAIVALCAGFLFNINPLLNFWVGSICGLGMLCVYAFWVPAKQKEELKKEADPNAAPTNPSFKEMISVLKMPTLWVLIVF
MLFTNTFYTVFDQQMFPNYYASLFPTTEIGNATYGTLNSFQVFLESAMMGVVPIIMKKIGVRNSLLLGATVMFLRIGLCG
VFHDPVSVSIVKLFHSIEVPLFCLPAFRYFTLHFDTKLSATLYMVGFQIASQIGQVIFSTPMGALHDAMGDRPTFFTISG
IVLAALIYGFFVIKKDDQEVGGDPFYTDKQLKAQAAAKANA
>Mature_440_residues
ASATKSAWKNPSYLQSSFGIFMFFCSWGIWWSFFSRWLTDPTHGLGMTSAEQGQIYSINSLATLVIMFAYGAIQDQLGIK
RKLVIFVSAIAALVGPFVQFVYAPMLTAGGTTRFIGVLIGSIVLSSGFMAGCSLFEALTERYSRKFGFEYGQSRAWGSFG
YAIVALCAGFLFNINPLLNFWVGSICGLGMLCVYAFWVPAKQKEELKKEADPNAAPTNPSFKEMISVLKMPTLWVLIVFM
LFTNTFYTVFDQQMFPNYYASLFPTTEIGNATYGTLNSFQVFLESAMMGVVPIIMKKIGVRNSLLLGATVMFLRIGLCGV
FHDPVSVSIVKLFHSIEVPLFCLPAFRYFTLHFDTKLSATLYMVGFQIASQIGQVIFSTPMGALHDAMGDRPTFFTISGI
VLAALIYGFFVIKKDDQEVGGDPFYTDKQLKAQAAAKANA

Specific function: Proton symport transport system. Has an essential role in sucrose metabolism [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family [H]

Homologues:

Organism=Escherichia coli, GI1786538, Length=375, Percent_Identity=29.8666666666667, Blast_Score=189, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022814
- InterPro:   IPR000576
- InterPro:   IPR018457
- InterPro:   IPR020846
- InterPro:   IPR016196 [H]

Pfam domain/function: PF01306 LacY_symp [H]

EC number: NA

Molecular weight: Translated: 48756; Mature: 48625

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
ASATKSAWKNPSYLQSSFGIFMFFCSWGIWWSFFSRWLTDPTHGLGMTSAEQGQIYSIN
CCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHH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CCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; lactose [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactose [Periplasm] = Proton [Cytoplasm] + lactose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1435727 [H]