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The map label for this gene is mdeA [H]

Identifier: 213691653

GI number: 213691653

Start: 879810

End: 881105

Strand: Direct

Name: mdeA [H]

Synonym: Blon_0757

Alternate gene names: 213691653

Gene position: 879810-881105 (Clockwise)

Preceding gene: 213691652

Following gene: 213691654

Centisome position: 31.06

GC content: 58.87

Gene sequence:

>1296_bases
ATGGCGAGGGATTTCGTTACCGCGGCTATTCACGGCGGCTATGACCGGGCGGCTCACGGTGAGGCGGCGAACCCGCCGAT
TTACGCCAGTGCGGCGTTTGACATGGTCAGCGCCGCGCATGGTGACGCGCTGGGTGCCGGCGAAGCTGAGGGCTTCTCGT
ACTCGCGTGTGGCGAATCCCACTGTGGATGTGTTCGAGCGTCGTTTGGCGGAACTCGAAGGTGGTGTGAGCGCGGTGGCC
GTCGCCTCCGGCATGGCCGCGGTGTCGTATGCGCTGTTGTGTGCCGCCGAAGGTGGTGGTCGCATCATCTCTCCGACCAA
CCTGTACGGCGCTTCCGTCGATGCCATGGAGACCTTCTTCCCGCAGTTCGGAGTCCATGCCGATTTCGTGCGCGACATCA
ACGACCTCGATGAGGTCGCTTCGAAGATCGGGCCGGACACCCGTGCCATCTACACCGAATCCGTTGCAAATCCATCCACT
GAAATCACCGATATTGAACCATTGGCGGAACTCGCGCATCGCAACGGTATTCCGCTTATCGTCGACAATACCGTGCCTAC
CCCGTACCTCTTCCGGCCCATCGAGTTCGGAGCCGATATCGTGGTGCATTCCACCACGAAAGGCATCACCGGGCACGGCA
ACGCCATCGGCGGAGTGGTTATCGACGGCGGAAACTTCGATTGGGCCAACGGCAGATTCCCGCAGTTCACCACTCCCGAA
CTGGTCGTCAGCGATGAACGCAAAGGAATAGCGCGCAGCTTCGCCAGCAGATTCGGCCGCGAGGCGTTCATTCGCCGTGT
ACGCATCAAATATCTGCGCACTTTCGGCGCGGTGCCCAGCCCGTTCAATACATACCTGTCGATGGTGGGATTGGAGACAC
TGGCTGTGCGTGAACGGCAGGAAGTCGAATCGGCCATGCATATTGCCGAGCATCTCACGCATGCGCCGCATGTGCTCAAG
GTCAATTATTCCGGTTTGGGCAACACGGGTCAGTATGATCTGGTCGCCAAATACTTCCCCAAAGGGATCGGTACGATACT
GTCGTTCCTCGTCGACGGCGATGAGAACAACGTGAGACGCATTCTCGACGGAACCGAGGTATTCTCCTATGTGCCGAACA
TCGGTGACGCGCGTTCGCTGATTGTCGATCCGGCGCGCATCACCCATCGTGAAGTGCCGTTGGAATATCGGCATGCCGCG
GGCGTAAGCGACAACCTTATCCGCCTGTCCATCGGATTGGAAAACGTCGATGATTTGATTGCGGACCTTGACCAAGCCAT
TGCCAACGCATACTGA

Upstream 100 bases:

>100_bases
CGTGTTACGGTGCTTCGCTTCCTACACTGGTGCCGTATCAGTATGGGCGTCCGTCGGTGTTCCCCTAAGGGACGGCGGCA
TGACATTGAGGGGATAATTC

Downstream 100 bases:

>100_bases
CGTGTATCGCTTACGTATGCGGATAATTGTTCGTTCTGAGAAAAACCTGCGCTGTTATTCTTGAGGCGTTGTGAATTTGG
GTTGTTGTATTACGAAAGAA

Product: Cysteine synthase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MARDFVTAAIHGGYDRAAHGEAANPPIYASAAFDMVSAAHGDALGAGEAEGFSYSRVANPTVDVFERRLAELEGGVSAVA
VASGMAAVSYALLCAAEGGGRIISPTNLYGASVDAMETFFPQFGVHADFVRDINDLDEVASKIGPDTRAIYTESVANPST
EITDIEPLAELAHRNGIPLIVDNTVPTPYLFRPIEFGADIVVHSTTKGITGHGNAIGGVVIDGGNFDWANGRFPQFTTPE
LVVSDERKGIARSFASRFGREAFIRRVRIKYLRTFGAVPSPFNTYLSMVGLETLAVRERQEVESAMHIAEHLTHAPHVLK
VNYSGLGNTGQYDLVAKYFPKGIGTILSFLVDGDENNVRRILDGTEVFSYVPNIGDARSLIVDPARITHREVPLEYRHAA
GVSDNLIRLSIGLENVDDLIADLDQAIANAY

Sequences:

>Translated_431_residues
MARDFVTAAIHGGYDRAAHGEAANPPIYASAAFDMVSAAHGDALGAGEAEGFSYSRVANPTVDVFERRLAELEGGVSAVA
VASGMAAVSYALLCAAEGGGRIISPTNLYGASVDAMETFFPQFGVHADFVRDINDLDEVASKIGPDTRAIYTESVANPST
EITDIEPLAELAHRNGIPLIVDNTVPTPYLFRPIEFGADIVVHSTTKGITGHGNAIGGVVIDGGNFDWANGRFPQFTTPE
LVVSDERKGIARSFASRFGREAFIRRVRIKYLRTFGAVPSPFNTYLSMVGLETLAVRERQEVESAMHIAEHLTHAPHVLK
VNYSGLGNTGQYDLVAKYFPKGIGTILSFLVDGDENNVRRILDGTEVFSYVPNIGDARSLIVDPARITHREVPLEYRHAA
GVSDNLIRLSIGLENVDDLIADLDQAIANAY
>Mature_430_residues
ARDFVTAAIHGGYDRAAHGEAANPPIYASAAFDMVSAAHGDALGAGEAEGFSYSRVANPTVDVFERRLAELEGGVSAVAV
ASGMAAVSYALLCAAEGGGRIISPTNLYGASVDAMETFFPQFGVHADFVRDINDLDEVASKIGPDTRAIYTESVANPSTE
ITDIEPLAELAHRNGIPLIVDNTVPTPYLFRPIEFGADIVVHSTTKGITGHGNAIGGVVIDGGNFDWANGRFPQFTTPEL
VVSDERKGIARSFASRFGREAFIRRVRIKYLRTFGAVPSPFNTYLSMVGLETLAVRERQEVESAMHIAEHLTHAPHVLKV
NYSGLGNTGQYDLVAKYFPKGIGTILSFLVDGDENNVRRILDGTEVFSYVPNIGDARSLIVDPARITHREVPLEYRHAAG
VSDNLIRLSIGLENVDDLIADLDQAIANAY

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=432, Percent_Identity=32.4074074074074, Blast_Score=188, Evalue=9e-48,
Organism=Homo sapiens, GI299473759, Length=432, Percent_Identity=31.25, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI25453487, Length=431, Percent_Identity=28.538283062645, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1790375, Length=424, Percent_Identity=30.8962264150943, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1789383, Length=407, Percent_Identity=27.2727272727273, Blast_Score=128, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17538055, Length=430, Percent_Identity=32.5581395348837, Blast_Score=187, Evalue=9e-48,
Organism=Caenorhabditis elegans, GI17560056, Length=384, Percent_Identity=34.1145833333333, Blast_Score=179, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI71980708, Length=407, Percent_Identity=29.7297297297297, Blast_Score=163, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6323333, Length=447, Percent_Identity=35.7941834451902, Blast_Score=247, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6319307, Length=434, Percent_Identity=32.4884792626728, Blast_Score=199, Evalue=6e-52,
Organism=Saccharomyces cerevisiae, GI6321254, Length=449, Percent_Identity=26.5033407572383, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321170, Length=196, Percent_Identity=29.0816326530612, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6321904, Length=435, Percent_Identity=25.9770114942529, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19922554, Length=426, Percent_Identity=32.6291079812207, Blast_Score=201, Evalue=9e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46352; Mature: 46220

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDFVTAAIHGGYDRAAHGEAANPPIYASAAFDMVSAAHGDALGAGEAEGFSYSRVANP
CCCHHEEHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCC
TVDVFERRLAELEGGVSAVAVASGMAAVSYALLCAAEGGGRIISPTNLYGASVDAMETFF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCCCCCCHHHHHHHH
PQFGVHADFVRDINDLDEVASKIGPDTRAIYTESVANPSTEITDIEPLAELAHRNGIPLI
HHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHCCCCCCCCCCHHHHHHHHHCCCCCEE
VDNTVPTPYLFRPIEFGADIVVHSTTKGITGHGNAIGGVVIDGGNFDWANGRFPQFTTPE
ECCCCCCCCEEEHHHCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
LVVSDERKGIARSFASRFGREAFIRRVRIKYLRTFGAVPSPFNTYLSMVGLETLAVRERQ
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
EVESAMHIAEHLTHAPHVLKVNYSGLGNTGQYDLVAKYFPKGIGTILSFLVDGDENNVRR
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
ILDGTEVFSYVPNIGDARSLIVDPARITHREVPLEYRHAAGVSDNLIRLSIGLENVDDLI
HHHHHHHHHHCCCCCCCHHEEECCHHHHHHCCCCHHHHHCCCCCCEEEEEECCCHHHHHH
ADLDQAIANAY
HHHHHHHHCCC
>Mature Secondary Structure 
ARDFVTAAIHGGYDRAAHGEAANPPIYASAAFDMVSAAHGDALGAGEAEGFSYSRVANP
CCHHEEHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCC
TVDVFERRLAELEGGVSAVAVASGMAAVSYALLCAAEGGGRIISPTNLYGASVDAMETFF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCCCCCCHHHHHHHH
PQFGVHADFVRDINDLDEVASKIGPDTRAIYTESVANPSTEITDIEPLAELAHRNGIPLI
HHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHCCCCCCCCCCHHHHHHHHHCCCCCEE
VDNTVPTPYLFRPIEFGADIVVHSTTKGITGHGNAIGGVVIDGGNFDWANGRFPQFTTPE
ECCCCCCCCEEEHHHCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
LVVSDERKGIARSFASRFGREAFIRRVRIKYLRTFGAVPSPFNTYLSMVGLETLAVRERQ
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
EVESAMHIAEHLTHAPHVLKVNYSGLGNTGQYDLVAKYFPKGIGTILSFLVDGDENNVRR
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
ILDGTEVFSYVPNIGDARSLIVDPARITHREVPLEYRHAAGVSDNLIRLSIGLENVDDLI
HHHHHHHHHHCCCCCCCHHEEECCHHHHHHCCCCHHHHHCCCCCCEEEEEECCCHHHHHH
ADLDQAIANAY
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]