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The map label for this gene is patB [H]

Identifier: 213691551

GI number: 213691551

Start: 752369

End: 753580

Strand: Reverse

Name: patB [H]

Synonym: Blon_0652

Alternate gene names: 213691551

Gene position: 753580-752369 (Counterclockwise)

Preceding gene: 213691557

Following gene: 213691545

Centisome position: 26.6

GC content: 62.05

Gene sequence:

>1212_bases
ATGACGTATGATTTCACGTCCATCATGAACAGGCATGGCATGGATTCGATCGCGGTCGACGGACTGGGCACCGACCCGGG
ATTCGCGCCGGACCCGCCACGCGAGGGTTTCGACGTCATCCCCATGTGGGTGGCGGACATGAACTTCCCGACCGTGCCCA
CGATCCCGCAGGCCATCATCGAACGGGCGAAGCATCCCGCCTACGGGTACTTCTCCCCCACCGACGACTATTACGGCTCC
ATCATCGACTGGCAGTCCACGCGCAACAGGGTCACCGGATTGACCCGGGAGGCGATCGGCTACGAGAACGGGGTCCTGGG
CGGCGTGATCAGCGCGCTGACCGCGTTCGCCGCCCCCGGCGACGGCGTGCTGCTGCATAGCCCGACCTATATCGGTTTCA
CCCGGAGTATCGAAAACAACGGCTACCGCATCGTCCACAGCCCGCTCACGCTGGACGAGCACGGCGTATGGCGCATGGAT
TACGAGGATATGGACAGGAAGCTCAAGGAGCATCACATCCACGTGGCCGTCTTCTGCAGTCCCCACAATCCCTGCGGGCG
CGTATGGGAGCGTTGGGAGATCGAGAAGGCCATGGAGGTGTACCGGGCCAACGACTGCGTGGTGATCTCCGACGAAATCT
GGTCGGATCTGACGTTGGGCGACCACCAGCACATCCCCACCCAATCGGTCAGCGAGGACGCACGCAACAGGACCGTGGCG
TTCTACGCGCCCAGCAAGACCTTCAATCTCGCCGGACTGGTGGGAAGCTACCACATCATCTACAATCCCACGCTCCGGGA
CCGCATCGTGGCCAAAAGCTCGAAATGCCACTACAACGACATGAACGTGCTTTCCATGCACGCGCTGATCGGCGCCTACC
GGCCCGAAGGCCATGAGTGGCTCGACGAATTGAAGGCGGTGCTCACCGGCAACGTCGATTACGCCTACGACTACATCACC
GGGCATTTCCAAGGCGTGAGTCTGTCCAAACCGGAAGGCACCTACATGCTGCTGCTCAACTGCGAGCAGTGGTGCGAACG
CCACGGCCTGAGCCTGCCTGAGTTGGAGAAACGCGGCTGGGATGTGGGCGTCGCATGGCAGGACGGCGACATGTTCCAGG
CCCCCTACAGCATCCGCGTCAATCTCGCATTGCCCTTGAGCCGCGTCAAGGAGGCCATGCGCCGCCTTGACCGGTACGTG
TTCAACGCCTGA

Upstream 100 bases:

>100_bases
CACGTCACGCGGTCAAATCGCCGCCCACGGCCACGGCACCGTCAGGAGCCCGATTTAGATTGAGACCAGCCACCACGGAC
CGAGAAAGGATTGTCGGACA

Downstream 100 bases:

>100_bases
TCGATGTGCCGGCGCGTCGGATCCCACGCGCCGGCACATCGCGGCGAGCCCACGGCCCGGGGCTTTCCTCAGCCCTTGAC
GGCGGTGGATTCGCTCACTT

Product: Aspartate transaminase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTYDFTSIMNRHGMDSIAVDGLGTDPGFAPDPPREGFDVIPMWVADMNFPTVPTIPQAIIERAKHPAYGYFSPTDDYYGS
IIDWQSTRNRVTGLTREAIGYENGVLGGVISALTAFAAPGDGVLLHSPTYIGFTRSIENNGYRIVHSPLTLDEHGVWRMD
YEDMDRKLKEHHIHVAVFCSPHNPCGRVWERWEIEKAMEVYRANDCVVISDEIWSDLTLGDHQHIPTQSVSEDARNRTVA
FYAPSKTFNLAGLVGSYHIIYNPTLRDRIVAKSSKCHYNDMNVLSMHALIGAYRPEGHEWLDELKAVLTGNVDYAYDYIT
GHFQGVSLSKPEGTYMLLLNCEQWCERHGLSLPELEKRGWDVGVAWQDGDMFQAPYSIRVNLALPLSRVKEAMRRLDRYV
FNA

Sequences:

>Translated_403_residues
MTYDFTSIMNRHGMDSIAVDGLGTDPGFAPDPPREGFDVIPMWVADMNFPTVPTIPQAIIERAKHPAYGYFSPTDDYYGS
IIDWQSTRNRVTGLTREAIGYENGVLGGVISALTAFAAPGDGVLLHSPTYIGFTRSIENNGYRIVHSPLTLDEHGVWRMD
YEDMDRKLKEHHIHVAVFCSPHNPCGRVWERWEIEKAMEVYRANDCVVISDEIWSDLTLGDHQHIPTQSVSEDARNRTVA
FYAPSKTFNLAGLVGSYHIIYNPTLRDRIVAKSSKCHYNDMNVLSMHALIGAYRPEGHEWLDELKAVLTGNVDYAYDYIT
GHFQGVSLSKPEGTYMLLLNCEQWCERHGLSLPELEKRGWDVGVAWQDGDMFQAPYSIRVNLALPLSRVKEAMRRLDRYV
FNA
>Mature_402_residues
TYDFTSIMNRHGMDSIAVDGLGTDPGFAPDPPREGFDVIPMWVADMNFPTVPTIPQAIIERAKHPAYGYFSPTDDYYGSI
IDWQSTRNRVTGLTREAIGYENGVLGGVISALTAFAAPGDGVLLHSPTYIGFTRSIENNGYRIVHSPLTLDEHGVWRMDY
EDMDRKLKEHHIHVAVFCSPHNPCGRVWERWEIEKAMEVYRANDCVVISDEIWSDLTLGDHQHIPTQSVSEDARNRTVAF
YAPSKTFNLAGLVGSYHIIYNPTLRDRIVAKSSKCHYNDMNVLSMHALIGAYRPEGHEWLDELKAVLTGNVDYAYDYITG
HFQGVSLSKPEGTYMLLLNCEQWCERHGLSLPELEKRGWDVGVAWQDGDMFQAPYSIRVNLALPLSRVKEAMRRLDRYVF
NA

Specific function: Catalyzes the transformation of cystathionine to homocysteine. Also exhibits cysteine desulfhydrase activity in vitro, producing sulfide from cysteine [H]

COG id: COG1168

COG function: function code E; Bifunctional PLP-dependent enzyme with beta-cystathionase and maltose regulon repressor activities

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. MalY/PatB cystathionine beta-lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787909, Length=340, Percent_Identity=30, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788627, Length=255, Percent_Identity=25.0980392156863, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322401, Length=308, Percent_Identity=23.3766233766234, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 45654; Mature: 45523

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYDFTSIMNRHGMDSIAVDGLGTDPGFAPDPPREGFDVIPMWVADMNFPTVPTIPQAII
CCCCHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHH
ERAKHPAYGYFSPTDDYYGSIIDWQSTRNRVTGLTREAIGYENGVLGGVISALTAFAAPG
HHCCCCCCCCCCCCCCCCCCEEECHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHCCCC
DGVLLHSPTYIGFTRSIENNGYRIVHSPLTLDEHGVWRMDYEDMDRKLKEHHIHVAVFCS
CCEEEECCCEEEEEEEECCCCEEEEECCCEECCCCEEEECHHHHHHHHHHCEEEEEEEEC
PHNPCGRVWERWEIEKAMEVYRANDCVVISDEIWSDLTLGDHQHIPTQSVSEDARNRTVA
CCCHHHHHHHHHHHHHHHHHHCCCCCEEECHHHCCCCCCCCCCCCCCCHHHHHHCCCEEE
FYAPSKTFNLAGLVGSYHIIYNPTLRDRIVAKSSKCHYNDMNVLSMHALIGAYRPEGHEW
EEECCCCCEEEEHHCCEEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHH
LDELKAVLTGNVDYAYDYITGHFQGVSLSKPEGTYMLLLNCEQWCERHGLSLPELEKRGW
HHHHHHHHCCCCCEEEEEEECEECCCCCCCCCCCEEEEEEHHHHHHHCCCCCCCHHHCCC
DVGVAWQDGDMFQAPYSIRVNLALPLSRVKEAMRRLDRYVFNA
EEEEEEECCCEEECCEEEEEEEECCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TYDFTSIMNRHGMDSIAVDGLGTDPGFAPDPPREGFDVIPMWVADMNFPTVPTIPQAII
CCCHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHH
ERAKHPAYGYFSPTDDYYGSIIDWQSTRNRVTGLTREAIGYENGVLGGVISALTAFAAPG
HHCCCCCCCCCCCCCCCCCCEEECHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHCCCC
DGVLLHSPTYIGFTRSIENNGYRIVHSPLTLDEHGVWRMDYEDMDRKLKEHHIHVAVFCS
CCEEEECCCEEEEEEEECCCCEEEEECCCEECCCCEEEECHHHHHHHHHHCEEEEEEEEC
PHNPCGRVWERWEIEKAMEVYRANDCVVISDEIWSDLTLGDHQHIPTQSVSEDARNRTVA
CCCHHHHHHHHHHHHHHHHHHCCCCCEEECHHHCCCCCCCCCCCCCCCHHHHHHCCCEEE
FYAPSKTFNLAGLVGSYHIIYNPTLRDRIVAKSSKCHYNDMNVLSMHALIGAYRPEGHEW
EEECCCCCEEEEHHCCEEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHH
LDELKAVLTGNVDYAYDYITGHFQGVSLSKPEGTYMLLLNCEQWCERHGLSLPELEKRGW
HHHHHHHHCCCCCEEEEEEECEECCCCCCCCCCCEEEEEEHHHHHHHCCCCCCCHHHCCC
DVGVAWQDGDMFQAPYSIRVNLALPLSRVKEAMRRLDRYVFNA
EEEEEEECCCEEECCEEEEEEEECCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497199; 9274030; 9384377 [H]