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The map label for this gene is trmB

Identifier: 213691442

GI number: 213691442

Start: 603713

End: 604573

Strand: Reverse

Name: trmB

Synonym: Blon_0537

Alternate gene names: 213691442

Gene position: 604573-603713 (Counterclockwise)

Preceding gene: 213691444

Following gene: 213691440

Centisome position: 21.34

GC content: 65.04

Gene sequence:

>861_bases
ATGACCGATCCCGAATCCACAGCAATCGATTCCGTCGCCGCGATGGCCACAGACCACACCGAGGCACCGGCGCACCCCTT
GCACAAGGTGCTCTCCTTCGTACGCCGCTCCGGACGTTTGGACGATCGCCTGCAGCGCGCATGGGACAACTATGCCGGCA
CATATCTGTTGGATATTGCGGCCGGCAATCTGCTGGACGTGCGCGAAGGCGTCACGCTCGACCGCGCGCTCGTGGAGTCC
GCATGGGGCAACGACAATCCACTCATTGTGGAAATCGGCACTGGACAGGGTGAGAACGTGGTGGCCGCAGCCGCGGCCCA
TCCGGAGACGAACTTCCTTGCCCTAGAGGTCTATGACCCCGGTGTGGCCCACACGCTGCTGCTCGCCGGCAAGCAGGGGC
TGACCAACATTCGCGTGGCCCAGGTCAATGCGCCCGAGCTGTTCAAGGTCACCGCGCCCGGCACCGCAGCCGAAGTATGG
ACCTTCTTCCCCGACCCCTGGCCCAAGAAGAAGCACCACAAGCGCCGCATCGTGCAGGAGGCCATGGCCGGCGACATCCA
TCGCGCACTGGCCGCAGACGGCGTCTGGCGCATCGCCACCGACATCGAGGACTATGCCCTGCACGTGCACGAGGTGATGG
ACGGACTCGACGGTTGGAAGAACCTGGGCAGCGTAACCGTCAGCCTCCCGCTGGAGCACGTGGGCAAGGGCAACGCCGAT
CTGGCCGCCGACATGCCGCACGCCGACTTCACCGAATCCGAGCGGTTCGAGGGCCGCGTGCTCACCAACTTCGAGAAGAA
GGGCCTGGCCGCCGGCCGCGTGATTCACGACTTCACCTATCAGGCCGTCGCCCTGCACTGA

Upstream 100 bases:

>100_bases
TGATATGGTGGTTGGTATCGCACACCACCTACCTAATGAACGCCAAATCGACCTAATGAATGAACAGCGGTGCGGCGCAC
AAGGAAAATCCGAGGAATAT

Downstream 100 bases:

>100_bases
TTGCGACACGCCGAAATACCGTCGATTTGCATAACCGAACAATCTCCCGTAAAGTAACTCGAGTTGTTCGCCCCCATCGT
CTAACGGTTAGGACACCAGA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MTDPESTAIDSVAAMATDHTEAPAHPLHKVLSFVRRSGRLDDRLQRAWDNYAGTYLLDIAAGNLLDVREGVTLDRALVES
AWGNDNPLIVEIGTGQGENVVAAAAAHPETNFLALEVYDPGVAHTLLLAGKQGLTNIRVAQVNAPELFKVTAPGTAAEVW
TFFPDPWPKKKHHKRRIVQEAMAGDIHRALAADGVWRIATDIEDYALHVHEVMDGLDGWKNLGSVTVSLPLEHVGKGNAD
LAADMPHADFTESERFEGRVLTNFEKKGLAAGRVIHDFTYQAVALH

Sequences:

>Translated_286_residues
MTDPESTAIDSVAAMATDHTEAPAHPLHKVLSFVRRSGRLDDRLQRAWDNYAGTYLLDIAAGNLLDVREGVTLDRALVES
AWGNDNPLIVEIGTGQGENVVAAAAAHPETNFLALEVYDPGVAHTLLLAGKQGLTNIRVAQVNAPELFKVTAPGTAAEVW
TFFPDPWPKKKHHKRRIVQEAMAGDIHRALAADGVWRIATDIEDYALHVHEVMDGLDGWKNLGSVTVSLPLEHVGKGNAD
LAADMPHADFTESERFEGRVLTNFEKKGLAAGRVIHDFTYQAVALH
>Mature_285_residues
TDPESTAIDSVAAMATDHTEAPAHPLHKVLSFVRRSGRLDDRLQRAWDNYAGTYLLDIAAGNLLDVREGVTLDRALVESA
WGNDNPLIVEIGTGQGENVVAAAAAHPETNFLALEVYDPGVAHTLLLAGKQGLTNIRVAQVNAPELFKVTAPGTAAEVWT
FFPDPWPKKKHHKRRIVQEAMAGDIHRALAADGVWRIATDIEDYALHVHEVMDGLDGWKNLGSVTVSLPLEHVGKGNADL
AADMPHADFTESERFEGRVLTNFEKKGLAAGRVIHDFTYQAVALH

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=259, Percent_Identity=33.976833976834, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_BIFLI (B7GP38)

Other databases:

- EMBL:   CP001095
- RefSeq:   YP_002322028.1
- ProteinModelPortal:   B7GP38
- GeneID:   7053197
- GenomeReviews:   CP001095_GR
- KEGG:   bln:Blon_0537
- HOGENOM:   HBG322782
- ProtClustDB:   CLSK573176
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 31140; Mature: 31009

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: BINDING 91-91 BINDING 116-116 BINDING 143-143 BINDING 165-165 BINDING 169-169 BINDING 201-201

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPESTAIDSVAAMATDHTEAPAHPLHKVLSFVRRSGRLDDRLQRAWDNYAGTYLLDIA
CCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEC
AGNLLDVREGVTLDRALVESAWGNDNPLIVEIGTGQGENVVAAAAAHPETNFLALEVYDP
CCCEEEHHCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECCCCCCEEEEEEECC
GVAHTLLLAGKQGLTNIRVAQVNAPELFKVTAPGTAAEVWTFFPDPWPKKKHHKRRIVQE
CHHHHHHHHCCCCCCEEEEEEECCCCEEEEECCCCHHHHEEECCCCCCCHHHHHHHHHHH
AMAGDIHRALAADGVWRIATDIEDYALHVHEVMDGLDGWKNLGSVTVSLPLEHVGKGNAD
HHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHCCCCCCC
LAADMPHADFTESERFEGRVLTNFEKKGLAAGRVIHDFTYQAVALH
EECCCCCCCCCCCCCCCCCEEECHHHCCCHHCHHHHHCCCEEEEEC
>Mature Secondary Structure 
TDPESTAIDSVAAMATDHTEAPAHPLHKVLSFVRRSGRLDDRLQRAWDNYAGTYLLDIA
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEC
AGNLLDVREGVTLDRALVESAWGNDNPLIVEIGTGQGENVVAAAAAHPETNFLALEVYDP
CCCEEEHHCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECCCCCCEEEEEEECC
GVAHTLLLAGKQGLTNIRVAQVNAPELFKVTAPGTAAEVWTFFPDPWPKKKHHKRRIVQE
CHHHHHHHHCCCCCCEEEEEEECCCCEEEEECCCCHHHHEEECCCCCCCHHHHHHHHHHH
AMAGDIHRALAADGVWRIATDIEDYALHVHEVMDGLDGWKNLGSVTVSLPLEHVGKGNAD
HHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHCCCCCCC
LAADMPHADFTESERFEGRVLTNFEKKGLAAGRVIHDFTYQAVALH
EECCCCCCCCCCCCCCCCCEEECHHHCCCHHCHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA