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The map label for this gene is femB [H]

Identifier: 213691327

GI number: 213691327

Start: 475618

End: 476928

Strand: Direct

Name: femB [H]

Synonym: Blon_0414

Alternate gene names: 213691327

Gene position: 475618-476928 (Clockwise)

Preceding gene: 213691326

Following gene: 213691328

Centisome position: 16.79

GC content: 59.8

Gene sequence:

>1311_bases
ATGACGCAATCCGATGCAGGCGTCGCCTCGCTCACCCCTACCAAGATCACTCCTGCCGAGCTGGATGCGTTCTCCGCAGC
TCAAGTGCAAGGTAGTTTCCAGCAGACCTCGCAGATGGCCGCCATGGGTGCGTTCAACGGTCCGGTGGACTATGTGGGTG
TCAAGCGCGGTGACAAGCTGATTGCCGCCTGCTACGTGGTGTACACGCGGTCCCGTTTCGGTTTGGAAGGCTCGGTCTGG
TGCGGACCGCTGTGCGATTATGACAATCCACAGGTGGTTGAGGCGATGACCGAAGCCCTGCGTCGTTCGGCCAAGGCCCA
TCACGCCATCAGCGTATCCTGCTGGCCGGCCGCAGTCTACCGTCTGCATGATCTGGACGGCAAGCCGACCAGCGACCCGG
ACACCACGATGATGGACAACATGGCCCGCTTCGGTTGGAAACATGGCGGCTTCACCGTGGGCTACGAATCCGTGGTGAAC
CGCTGGAACTTCGTCAAGGGGCTTGACGGCATTCATAACGAGAAGGAACTGCTGGCCTCGTTCTCCAAGTACCGCCGTAA
GAACATCCGCATCGCCCAGGATTCCGGTCTGCGCGTGCGCCGACTGGAACGCGGCGAACTGAGCATTTTCGTCAAGCTGT
GCGATATGAGCGCCGCACGTCAAGGCTTCAAGAGCCGCGATCTGGCCTACTATGAGCGTCTGTTCGATACCTTCGGCGAC
CTGATCGAGTTCAAGGTGGTCGAAACCCATTTCGACGAATATCTGGACACCCTGCAATCCAAGCTGAATGCGGCCTCCAA
AGACAAGCGCAACCTCGAACGCCTGCTCCAGCGCGCGCAACAGCAGCCGGAAGGCACGGCCAAGAAGGGTGCCTCCGACC
CGGCAACGCTGGAAAAGCGCATCGCCACCGCAGACAAGAAGATTGCGGCGCTGGAAAAGACCATCGGCGAAGTCAATGGC
ATCATCGCCTCGGACGGCCCGGTCATTCCGGTGGAAGCCGGTGTCTACTTCTGGCATCCGAACGAGGTCGTCTGCCTGTC
CAGCGGCGAGGACGATCGTTTCATGGATTACTACCCGTCTTCCCTAATCCACTTCGAGGCGATGCTTGACTGCATCGCCC
GTGGCGTGGAGCGCTTCAACTTCTATGGCATTTCCGGCATTTTCGACGCCAACGATCCTTCCTACGGCGTCTGGCAGTTC
AAGACTCGCTTCGGCGGATTCGTGGAAGAGCTTCCGGGCAAGTTCACGCTGCCGGTGGACGGACTGCGCTTCGGTGTATC
CGAAGCCGCACACAAGCTGCTGCATCGCTGA

Upstream 100 bases:

>100_bases
TTTGCGCACAAGCTGCTGTCGCGGTAAACGGACGCAGCACACAGGCCATTCCAGAGCCCAGAGTAACGATCAGAGTAACG
ACAAGTAAGAAGGTTCGATT

Downstream 100 bases:

>100_bases
CAAACAGTGGCGAGCACGGACACAGAAATAAGGCACAGGTGACGACGAGATGAGCGCGACTGTGAATCGAACAGATACGA
CGTATACGTTTGGCGTGATT

Product: Methicillin resistance protein

Products: NA

Alternate protein names: Factor essential for expression of methicillin resistance B [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MTQSDAGVASLTPTKITPAELDAFSAAQVQGSFQQTSQMAAMGAFNGPVDYVGVKRGDKLIAACYVVYTRSRFGLEGSVW
CGPLCDYDNPQVVEAMTEALRRSAKAHHAISVSCWPAAVYRLHDLDGKPTSDPDTTMMDNMARFGWKHGGFTVGYESVVN
RWNFVKGLDGIHNEKELLASFSKYRRKNIRIAQDSGLRVRRLERGELSIFVKLCDMSAARQGFKSRDLAYYERLFDTFGD
LIEFKVVETHFDEYLDTLQSKLNAASKDKRNLERLLQRAQQQPEGTAKKGASDPATLEKRIATADKKIAALEKTIGEVNG
IIASDGPVIPVEAGVYFWHPNEVVCLSSGEDDRFMDYYPSSLIHFEAMLDCIARGVERFNFYGISGIFDANDPSYGVWQF
KTRFGGFVEELPGKFTLPVDGLRFGVSEAAHKLLHR

Sequences:

>Translated_436_residues
MTQSDAGVASLTPTKITPAELDAFSAAQVQGSFQQTSQMAAMGAFNGPVDYVGVKRGDKLIAACYVVYTRSRFGLEGSVW
CGPLCDYDNPQVVEAMTEALRRSAKAHHAISVSCWPAAVYRLHDLDGKPTSDPDTTMMDNMARFGWKHGGFTVGYESVVN
RWNFVKGLDGIHNEKELLASFSKYRRKNIRIAQDSGLRVRRLERGELSIFVKLCDMSAARQGFKSRDLAYYERLFDTFGD
LIEFKVVETHFDEYLDTLQSKLNAASKDKRNLERLLQRAQQQPEGTAKKGASDPATLEKRIATADKKIAALEKTIGEVNG
IIASDGPVIPVEAGVYFWHPNEVVCLSSGEDDRFMDYYPSSLIHFEAMLDCIARGVERFNFYGISGIFDANDPSYGVWQF
KTRFGGFVEELPGKFTLPVDGLRFGVSEAAHKLLHR
>Mature_435_residues
TQSDAGVASLTPTKITPAELDAFSAAQVQGSFQQTSQMAAMGAFNGPVDYVGVKRGDKLIAACYVVYTRSRFGLEGSVWC
GPLCDYDNPQVVEAMTEALRRSAKAHHAISVSCWPAAVYRLHDLDGKPTSDPDTTMMDNMARFGWKHGGFTVGYESVVNR
WNFVKGLDGIHNEKELLASFSKYRRKNIRIAQDSGLRVRRLERGELSIFVKLCDMSAARQGFKSRDLAYYERLFDTFGDL
IEFKVVETHFDEYLDTLQSKLNAASKDKRNLERLLQRAQQQPEGTAKKGASDPATLEKRIATADKKIAALEKTIGEVNGI
IASDGPVIPVEAGVYFWHPNEVVCLSSGEDDRFMDYYPSSLIHFEAMLDCIARGVERFNFYGISGIFDANDPSYGVWQFK
TRFGGFVEELPGKFTLPVDGLRFGVSEAAHKLLHR

Specific function: Catalyzes the formation of the pentaglycine interpeptide bridge, which is characteristic of the S.aureus peptidoglycan. Adds glycines 4 and 5 of the pentaglycine bridge, using glycyl- tRNA(Gly) as donor. Involved in resistance to methicillin [H]

COG id: COG2348

COG function: function code V; Uncharacterized protein involved in methicillin resistance

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the femABX family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016181
- InterPro:   IPR003447 [H]

Pfam domain/function: PF02388 FemAB [H]

EC number: NA

Molecular weight: Translated: 48585; Mature: 48453

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSDAGVASLTPTKITPAELDAFSAAQVQGSFQQTSQMAAMGAFNGPVDYVGVKRGDKL
CCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHH
IAACYVVYTRSRFGLEGSVWCGPLCDYDNPQVVEAMTEALRRSAKAHHAISVSCWPAAVY
HHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEEHHHHHHH
RLHDLDGKPTSDPDTTMMDNMARFGWKHGGFTVGYESVVNRWNFVKGLDGIHNEKELLAS
HHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHCCCCCCHHHHHHH
FSKYRRKNIRIAQDSGLRVRRLERGELSIFVKLCDMSAARQGFKSRDLAYYERLFDTFGD
HHHHHHCCCEEECCCCCEEEEECCCCEEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LIEFKVVETHFDEYLDTLQSKLNAASKDKRNLERLLQRAQQQPEGTAKKGASDPATLEKR
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHH
IATADKKIAALEKTIGEVNGIIASDGPVIPVEAGVYFWHPNEVVCLSSGEDDRFMDYYPS
HHHHHHHHHHHHHHHHHHCCEEECCCCEEEEECCEEEECCCEEEEEECCCCCCHHHHCCH
SLIHFEAMLDCIARGVERFNFYGISGIFDANDPSYGVWQFKTRFGGFVEELPGKFTLPVD
HHHHHHHHHHHHHHHHHHHEEECCCEEECCCCCCCCEEEEHHHHCHHHHHCCCCEECCCC
GLRFGVSEAAHKLLHR
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQSDAGVASLTPTKITPAELDAFSAAQVQGSFQQTSQMAAMGAFNGPVDYVGVKRGDKL
CCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHH
IAACYVVYTRSRFGLEGSVWCGPLCDYDNPQVVEAMTEALRRSAKAHHAISVSCWPAAVY
HHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEEHHHHHHH
RLHDLDGKPTSDPDTTMMDNMARFGWKHGGFTVGYESVVNRWNFVKGLDGIHNEKELLAS
HHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHCCCCCCHHHHHHH
FSKYRRKNIRIAQDSGLRVRRLERGELSIFVKLCDMSAARQGFKSRDLAYYERLFDTFGD
HHHHHHCCCEEECCCCCEEEEECCCCEEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LIEFKVVETHFDEYLDTLQSKLNAASKDKRNLERLLQRAQQQPEGTAKKGASDPATLEKR
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHH
IATADKKIAALEKTIGEVNGIIASDGPVIPVEAGVYFWHPNEVVCLSSGEDDRFMDYYPS
HHHHHHHHHHHHHHHHHHCCEEECCCCEEEEECCEEEECCCEEEEEECCCCCCHHHHCCH
SLIHFEAMLDCIARGVERFNFYGISGIFDANDPSYGVWQFKTRFGGFVEELPGKFTLPVD
HHHHHHHHHHHHHHHHHHHEEECCCEEECCCCCCCCEEEEHHHHCHHHHHCCCCEECCCC
GLRFGVSEAAHKLLHR
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA