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The map label for this gene is rph [H]

Identifier: 213691324

GI number: 213691324

Start: 472252

End: 473028

Strand: Direct

Name: rph [H]

Synonym: Blon_0411

Alternate gene names: 213691324

Gene position: 472252-473028 (Clockwise)

Preceding gene: 213691323

Following gene: 213691325

Centisome position: 16.67

GC content: 60.49

Gene sequence:

>777_bases
ATGCTGGGGCGCATGGTTGAAATCAAAGACATGCTTGGCAGTCACCAAATAATTCGTGCCGACGGCCGCAAGGTCGACGA
ACTGCGCCCCGTGCGCATCACCCGCCACTTCACCGACGCCCCCGAAGGCTCCGTGCTCATCGAATGCGGTAACACGCGCG
TGATGTGCACCGCCACTTTTACCCCCGGTGTGCCGCGTTGGCGCAAGGACTCCGGCCTCGGCTGGGTCACCGCCGAATAC
TCGATGCTGCCGCGTGCCACCGCCGAACGCACCGACCGTGAATCCGTACGTGGCAAAATCGGTGGCCGTACCCACGAAAT
CAGCCGACTGATTGGCCGCTGCCTGCGTGGCGTCATCGATATGAAGGCGCTCGGCGAAAATCAGATTCAGCTTGACTGCG
ATGTACTTCAGGCCGATGGCGGCACTCGCACGGCTTCCGTAACCGGTGCGTACGTGGCTTTGGTCGACGCAGTCAATTGG
GCGGAGAAGCACCGCCATATCAAGTCCGCCAGCCGCGTGCTTAAGGACTATGTGTCCGCCGTATCGGTGGGCGTGATCAA
CGGCACTCCGATGCTTGACCTGCCATACATTGAAGACAGTCAGGCCATGACCGACATGAACGTGGCCATGACCGGTTCCG
GCACCTTCATCGAGATTCAGGGCACCGCAGAACACCGTCCGTTCAACCGTGCCGAACTCGGCACCCTGCTTGACTTGGCC
GAAAAGGGCAACAAGGAACTGCAAGCCGCCCAGCGTGCCGCTCTGTCTTTAGACTAA

Upstream 100 bases:

>100_bases
ATCCAAGTGTCATGGGATGGATATGTAAGCAGGGAATGACATTGCGCTGGAGTTTGATGATGGTTGACGAGTTGCTATCC
AAGCATAGAGCGGATGGCGT

Downstream 100 bases:

>100_bases
GCCAGCTCCCCTGCCAAGGGGAGCCTTCTTAAAGGAGAATCCATGCAAATCGTCGTCGCAACCCATAACGAAGGCAAGCT
TGTGGAAATCCGCCGCATTC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLGRMVEIKDMLGSHQIIRADGRKVDELRPVRITRHFTDAPEGSVLIECGNTRVMCTATFTPGVPRWRKDSGLGWVTAEY
SMLPRATAERTDRESVRGKIGGRTHEISRLIGRCLRGVIDMKALGENQIQLDCDVLQADGGTRTASVTGAYVALVDAVNW
AEKHRHIKSASRVLKDYVSAVSVGVINGTPMLDLPYIEDSQAMTDMNVAMTGSGTFIEIQGTAEHRPFNRAELGTLLDLA
EKGNKELQAAQRAALSLD

Sequences:

>Translated_258_residues
MLGRMVEIKDMLGSHQIIRADGRKVDELRPVRITRHFTDAPEGSVLIECGNTRVMCTATFTPGVPRWRKDSGLGWVTAEY
SMLPRATAERTDRESVRGKIGGRTHEISRLIGRCLRGVIDMKALGENQIQLDCDVLQADGGTRTASVTGAYVALVDAVNW
AEKHRHIKSASRVLKDYVSAVSVGVINGTPMLDLPYIEDSQAMTDMNVAMTGSGTFIEIQGTAEHRPFNRAELGTLLDLA
EKGNKELQAAQRAALSLD
>Mature_258_residues
MLGRMVEIKDMLGSHQIIRADGRKVDELRPVRITRHFTDAPEGSVLIECGNTRVMCTATFTPGVPRWRKDSGLGWVTAEY
SMLPRATAERTDRESVRGKIGGRTHEISRLIGRCLRGVIDMKALGENQIQLDCDVLQADGGTRTASVTGAYVALVDAVNW
AEKHRHIKSASRVLKDYVSAVSVGVINGTPMLDLPYIEDSQAMTDMNVAMTGSGTFIEIQGTAEHRPFNRAELGTLLDLA
EKGNKELQAAQRAALSLD

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Homo sapiens, GI9506689, Length=202, Percent_Identity=28.2178217821782, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=57.345971563981, Blast_Score=242, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI71981632, Length=198, Percent_Identity=28.7878787878788, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24584046, Length=247, Percent_Identity=25.1012145748988, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 28248; Mature: 28248

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGRMVEIKDMLGSHQIIRADGRKVDELRPVRITRHFTDAPEGSVLIECGNTRVMCTATF
CCCCEEHHHHHHCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCEEEEEEEC
TPGVPRWRKDSGLGWVTAEYSMLPRATAERTDRESVRGKIGGRTHEISRLIGRCLRGVID
CCCCCCCCCCCCCEEEEECHHHCCCCHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHH
MKALGENQIQLDCDVLQADGGTRTASVTGAYVALVDAVNWAEKHRHIKSASRVLKDYVSA
HHHCCCCCEEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSVGVINGTPMLDLPYIEDSQAMTDMNVAMTGSGTFIEIQGTAEHRPFNRAELGTLLDLA
HHEEEECCCCCEECCCCCCCCHHCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHH
EKGNKELQAAQRAALSLD
HCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MLGRMVEIKDMLGSHQIIRADGRKVDELRPVRITRHFTDAPEGSVLIECGNTRVMCTATF
CCCCEEHHHHHHCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCEEEEEEEC
TPGVPRWRKDSGLGWVTAEYSMLPRATAERTDRESVRGKIGGRTHEISRLIGRCLRGVID
CCCCCCCCCCCCCEEEEECHHHCCCCHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHH
MKALGENQIQLDCDVLQADGGTRTASVTGAYVALVDAVNWAEKHRHIKSASRVLKDYVSA
HHHCCCCCEEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSVGVINGTPMLDLPYIEDSQAMTDMNVAMTGSGTFIEIQGTAEHRPFNRAELGTLLDLA
HHEEEECCCCCEECCCCCCCCHHCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHH
EKGNKELQAAQRAALSLD
HCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]