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The map label for this gene is ddl [H]

Identifier: 213691237

GI number: 213691237

Start: 370875

End: 372062

Strand: Direct

Name: ddl [H]

Synonym: Blon_0320

Alternate gene names: 213691237

Gene position: 370875-372062 (Clockwise)

Preceding gene: 213691236

Following gene: 213691238

Centisome position: 13.09

GC content: 61.78

Gene sequence:

>1188_bases
ATGGCCAAGAAGCGTGTTGTGGTGCTCTATGGTGGGCGTGCAGACGAGCATTCGATTTCCTGCATCTCCACTGCCGGTGT
GCTGGGGGCCATGGACACTGAACGGTTCGAGCCGATTCCGGTCGGCATCACCAAGGACGGCAAGTGGATCATCAACGGCG
AAGATCCTCGTGGCTGGAACTTGGACGGCGGCGAGCTGCCGACCGTAAAGATCACCCCGGAATCGCGCCCGGTGATGCTT
GACCCCTCTCGCGGCCAGGACGGTTTCTTCATCGGCGAGCCCAGCCACATCAATTCCGCCGATTCCGGCTTCGGCACCAG
CTTCGTCAGCATGCCCGACCCGGAAATGCACCATGCGCTGACCTCCCTTGGCCATGTGGACGCGGTACTGCCGGTGCTGC
ACGGCCCCTATGGCGAGGACGGCACGGTTCAAGGCCTGCTGGAAATGATGGGTGTGCCGTACGTGGGGTGCGGAGTGTTT
GCTTCCGCCGCCTGCATGGATAAGCATTACACCAAGGTGGTGCTGGGTGCCGCCGGCATTCCCACCGCGCCGGGTGTGAC
GGTCGATGCGCGCAACTTCACCGCCGCCGATGTGCTTGCCAAGATCGAGGACGCCGGTCTTACCTATCCGCTGTTCATCA
AGCCTTCGCGCGCAGGCTCCAGCTTCGGTGTGACCAAGGTCGAGAAAGCCGATGACCGCGAGACCCAGCAGGATCGTCTG
GCCGCCGCCATCGCCACCGCCGGCGAGCACGATTGGAAGGTGCTCGTCGAACAGGGGATTGATGGCCGCGAAATCGAATG
TGCCGTGCTGTGTCCGAAGGCCGGTGACGAGCCTGAAGCCAGCTGGCCCGGCGAAATCGTGCTCGATCATCAGAACGACG
ACCAGTTCTACGATTTCGACTCCAAGTACATGGATGCCTCCGCCAGCCACGTGGAAGTGCCTGCCAATCTGCCAGTCAGC
GTGCTTGAGGACGTGCGTGACGTGGCCCGGCGTGCGTTCAAGGCCGTGGATGGTGCCGGCTTGAGCCGTGTGGACACCTT
CGTGACGCCTGATGGCACGGTGATGGTCAACGAAATCAACACCATGCCCGGCTTCACGCCGATTTCTATGTATCCCAAGG
CTTGGGATGCCACCGGAGTGAGCTACACCGAACTCATCACGCGATTGATTGAGGGTGTGCTGAGGTAG

Upstream 100 bases:

>100_bases
TGTGTGTCTCTTGTGCGCGGCGGTCCGAGCGCGGTTCGGTCCGGTTGGCGGTGCAAAGCATAGGCTTGAAAACGGTTGTA
GTTTCAATAAAAGGATGGTT

Downstream 100 bases:

>100_bases
ATCGAGGGCGTGCCGCGGTAGGCGCTGCGCGGCGGGCGGATGACGTGGGTCGGCCGGTGGTCGGCCCACGGACGACACGT
CGTCCGTTGTCGTTGCCGTG

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MAKKRVVVLYGGRADEHSISCISTAGVLGAMDTERFEPIPVGITKDGKWIINGEDPRGWNLDGGELPTVKITPESRPVML
DPSRGQDGFFIGEPSHINSADSGFGTSFVSMPDPEMHHALTSLGHVDAVLPVLHGPYGEDGTVQGLLEMMGVPYVGCGVF
ASAACMDKHYTKVVLGAAGIPTAPGVTVDARNFTAADVLAKIEDAGLTYPLFIKPSRAGSSFGVTKVEKADDRETQQDRL
AAAIATAGEHDWKVLVEQGIDGREIECAVLCPKAGDEPEASWPGEIVLDHQNDDQFYDFDSKYMDASASHVEVPANLPVS
VLEDVRDVARRAFKAVDGAGLSRVDTFVTPDGTVMVNEINTMPGFTPISMYPKAWDATGVSYTELITRLIEGVLR

Sequences:

>Translated_395_residues
MAKKRVVVLYGGRADEHSISCISTAGVLGAMDTERFEPIPVGITKDGKWIINGEDPRGWNLDGGELPTVKITPESRPVML
DPSRGQDGFFIGEPSHINSADSGFGTSFVSMPDPEMHHALTSLGHVDAVLPVLHGPYGEDGTVQGLLEMMGVPYVGCGVF
ASAACMDKHYTKVVLGAAGIPTAPGVTVDARNFTAADVLAKIEDAGLTYPLFIKPSRAGSSFGVTKVEKADDRETQQDRL
AAAIATAGEHDWKVLVEQGIDGREIECAVLCPKAGDEPEASWPGEIVLDHQNDDQFYDFDSKYMDASASHVEVPANLPVS
VLEDVRDVARRAFKAVDGAGLSRVDTFVTPDGTVMVNEINTMPGFTPISMYPKAWDATGVSYTELITRLIEGVLR
>Mature_394_residues
AKKRVVVLYGGRADEHSISCISTAGVLGAMDTERFEPIPVGITKDGKWIINGEDPRGWNLDGGELPTVKITPESRPVMLD
PSRGQDGFFIGEPSHINSADSGFGTSFVSMPDPEMHHALTSLGHVDAVLPVLHGPYGEDGTVQGLLEMMGVPYVGCGVFA
SAACMDKHYTKVVLGAAGIPTAPGVTVDARNFTAADVLAKIEDAGLTYPLFIKPSRAGSSFGVTKVEKADDRETQQDRLA
AAIATAGEHDWKVLVEQGIDGREIECAVLCPKAGDEPEASWPGEIVLDHQNDDQFYDFDSKYMDASASHVEVPANLPVSV
LEDVRDVARRAFKAVDGAGLSRVDTFVTPDGTVMVNEINTMPGFTPISMYPKAWDATGVSYTELITRLIEGVLR

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=402, Percent_Identity=39.8009950248756, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI1786280, Length=275, Percent_Identity=35.6363636363636, Blast_Score=152, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 42306; Mature: 42175

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKRVVVLYGGRADEHSISCISTAGVLGAMDTERFEPIPVGITKDGKWIINGEDPRGWN
CCCCEEEEEECCCCCCCCEEEEECCCHHCCCCCCCCCCEEEEEECCCEEEECCCCCCCCC
LDGGELPTVKITPESRPVMLDPSRGQDGFFIGEPSHINSADSGFGTSFVSMPDPEMHHAL
CCCCCCCEEEECCCCCCEEECCCCCCCCEEECCCCCCCCCCCCCCCEECCCCCHHHHHHH
TSLGHVDAVLPVLHGPYGEDGTVQGLLEMMGVPYVGCGVFASAACMDKHYTKVVLGAAGI
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEECHHHHHHHHHHHHHCEEEEEECCCC
PTAPGVTVDARNFTAADVLAKIEDAGLTYPLFIKPSRAGSSFGVTKVEKADDRETQQDRL
CCCCCCEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEECCCCCHHHHHHHH
AAAIATAGEHDWKVLVEQGIDGREIECAVLCPKAGDEPEASWPGEIVLDHQNDDQFYDFD
HHHHHHCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCEEECC
SKYMDASASHVEVPANLPVSVLEDVRDVARRAFKAVDGAGLSRVDTFVTPDGTVMVNEIN
CHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHEEECCCCEEEEEECC
TMPGFTPISMYPKAWDATGVSYTELITRLIEGVLR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKKRVVVLYGGRADEHSISCISTAGVLGAMDTERFEPIPVGITKDGKWIINGEDPRGWN
CCCEEEEEECCCCCCCCEEEEECCCHHCCCCCCCCCCEEEEEECCCEEEECCCCCCCCC
LDGGELPTVKITPESRPVMLDPSRGQDGFFIGEPSHINSADSGFGTSFVSMPDPEMHHAL
CCCCCCCEEEECCCCCCEEECCCCCCCCEEECCCCCCCCCCCCCCCEECCCCCHHHHHHH
TSLGHVDAVLPVLHGPYGEDGTVQGLLEMMGVPYVGCGVFASAACMDKHYTKVVLGAAGI
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEECHHHHHHHHHHHHHCEEEEEECCCC
PTAPGVTVDARNFTAADVLAKIEDAGLTYPLFIKPSRAGSSFGVTKVEKADDRETQQDRL
CCCCCCEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEECCCCCHHHHHHHH
AAAIATAGEHDWKVLVEQGIDGREIECAVLCPKAGDEPEASWPGEIVLDHQNDDQFYDFD
HHHHHHCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCEEECC
SKYMDASASHVEVPANLPVSVLEDVRDVARRAFKAVDGAGLSRVDTFVTPDGTVMVNEIN
CHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHEEECCCCEEEEEECC
TMPGFTPISMYPKAWDATGVSYTELITRLIEGVLR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]