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The map label for this gene is repF [H]

Identifier: 213691212

GI number: 213691212

Start: 348722

End: 349132

Strand: Reverse

Name: repF [H]

Synonym: Blon_0295

Alternate gene names: 213691212

Gene position: 349132-348722 (Counterclockwise)

Preceding gene: 213691213

Following gene: 213691211

Centisome position: 12.32

GC content: 52.55

Gene sequence:

>411_bases
ATGAGGGACATGACTCATCAGAGAAAAGCTACGAACGAAAAAGGCAGGTACTGGGCTGGCCTAATCTATCCCGATAGTTG
TCCCGACGATTGGAAAGACCGAATGAAGATCAGCGGTCTGGAAATCCTTGTTTCTCCCGTACACGATCAGGACATTGCCG
ACATCGCCACTGGCGAACTGAAGAAGCCACACCGCCATGTAATCGCAATGTGGGCCAACACGACCACACGCCAGAACGCC
GAGCGTTTCTTTGAGCAATTCGGCGGCCCAAAGATGATCCTGCGATTGGAGAATCCCCGTGGCATGGCTCGATACCTGAT
CCACATGGATGATCCGGATAAGGCTCCATACAGTCCCGAAGACGTGCTCGAGTTCAACGGTGCGGACTGGAAAAAAAATC
GCACTGGTTGA

Upstream 100 bases:

>100_bases
CGGCACTGACGACGAAGCGGGCGAAGCGACAGCGGGAGCCCGTTCACACCCGACAAACAAGTTGTGGGGGCTACTACGAC
GCCCCCGCAAGGGATGAGAG

Downstream 100 bases:

>100_bases
AGGCAATCAATCCGACGCCATGTCCATTGTCAAACTGGTGGAGGAAGAGCGCATTCACGGATATTTCGATCTTCTGAAGG
CCTGTGAGGCTGACCACCCC

Product: plasmid replication protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 136; Mature: 136

Protein sequence:

>136_residues
MRDMTHQRKATNEKGRYWAGLIYPDSCPDDWKDRMKISGLEILVSPVHDQDIADIATGELKKPHRHVIAMWANTTTRQNA
ERFFEQFGGPKMILRLENPRGMARYLIHMDDPDKAPYSPEDVLEFNGADWKKNRTG

Sequences:

>Translated_136_residues
MRDMTHQRKATNEKGRYWAGLIYPDSCPDDWKDRMKISGLEILVSPVHDQDIADIATGELKKPHRHVIAMWANTTTRQNA
ERFFEQFGGPKMILRLENPRGMARYLIHMDDPDKAPYSPEDVLEFNGADWKKNRTG
>Mature_136_residues
MRDMTHQRKATNEKGRYWAGLIYPDSCPDDWKDRMKISGLEILVSPVHDQDIADIATGELKKPHRHVIAMWANTTTRQNA
ERFFEQFGGPKMILRLENPRGMARYLIHMDDPDKAPYSPEDVLEFNGADWKKNRTG

Specific function: Is essential for plasmid replication. Nicks the positive strand at the plus origin of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Gram-positive plasmids replication protein type 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002631 [H]

Pfam domain/function: PF01719 Rep_2 [H]

EC number: NA

Molecular weight: Translated: 15799; Mature: 15799

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDMTHQRKATNEKGRYWAGLIYPDSCPDDWKDRMKISGLEILVSPVHDQDIADIATGEL
CCCCCCHHHCCCCCCCEEEEEECCCCCCCCHHHHEECCCEEEEEECCCCCCHHHHHCCCC
KKPHRHVIAMWANTTTRQNAERFFEQFGGPKMILRLENPRGMARYLIHMDDPDKAPYSPE
CCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCHH
DVLEFNGADWKKNRTG
HHEECCCCCCCCCCCC
>Mature Secondary Structure
MRDMTHQRKATNEKGRYWAGLIYPDSCPDDWKDRMKISGLEILVSPVHDQDIADIATGEL
CCCCCCHHHCCCCCCCEEEEEECCCCCCCCHHHHEECCCEEEEEECCCCCCHHHHHCCCC
KKPHRHVIAMWANTTTRQNAERFFEQFGGPKMILRLENPRGMARYLIHMDDPDKAPYSPE
CCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCHH
DVLEFNGADWKKNRTG
HHEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2443486; 6279574 [H]