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The map label for this gene is gatB

Identifier: 213691180

GI number: 213691180

Start: 305218

End: 306717

Strand: Direct

Name: gatB

Synonym: Blon_0260

Alternate gene names: 213691180

Gene position: 305218-306717 (Clockwise)

Preceding gene: 213691179

Following gene: 213691181

Centisome position: 10.77

GC content: 63.47

Gene sequence:

>1500_bases
ATGGCTGAAAAACTGATGAAGTACGCCGATGCCACCAAGAAGTACGATGTGGTCTTCGGTCTGGAAACCCACGTGGAGCT
GTCCACGAACACCAAGCTGTTCTGCCCGGCCCACATCGAGTTCGGCGGCGAGCCGAACACCGAACTGACTCCGGTGAGCC
TCGGCCTGCCGGGCAGTCTGCCGGTGATCAACAAGACCGCGGTCGATTACGCCATCAAACTGGGTCTCGCCCTGCACTGC
GAAATCGCCGAGTGGAGCCAGTTCGCCCGCAAGAACTACTTCTACCCGGACATGCCGCGCGACTATCAGATCTCGCAGTA
CGACAAGCCCACCAACGGCAATGGCTACCTGGACGTCGAGCTGGAAGACGGCACTGTGTTCCGTGTGCCGATCGAGCGCG
CCCACATCGAAGACGACGCCGGCAAGAACACCCACGTGGGTGGTGCCGACGGCCGTATCGAAGGCGCCGACCACTCGCTG
GTCGACTACAACCGCGCCGGTGTGCCGCTGATCGAGATCGTCACCAAGCCGATCGAGGGTGCGGGCGACCGCGCTCCCGA
AATCGCCGGTGCCTATGTGCGCGCCATCCGCGACATCGTGCGTGCGCTGAACATCTCCCACGCTCGTATGGAGCAGGGCA
ACATGCGCGCCGACGTGAACGTCTCGCTGCGTCCAAGCCCGGATGCCCCCTACGGCACCCGTTCCGAGACCAAGAACGTG
AACTCGTTCCGCGGCATCGAGAAGACCATCCAGTACGAGATTCGCCGTCAGGCCGCTCGTCTGGACGACGGCAAGGAGAT
TCTGCAGGAGACCCGCCACTGGGATGAGGCTACGCAGACCACCGCTGGCGGCCGTCTGAAGTCCGATGCCGACGACTACC
GCTACTTCCCGGACCCCGACCTCGTCATGCTCCACATCACCAAGGAGCACATCGAGGAGATGAAGGCCCAGATGCCGGAA
ATGCCGCGCGAGCGCCGCAACCGCCTCAAGTCCGAGTGGGGCCTGAGTGATCTGCAGATGCGCGACATCCTCAACGCCGA
CGCCCTCGACCTGATTGAGGAAACCGTCAAGGCCGGTGCCAAGGCGGCCGGTGCGCGCAAGTGGTGGCTGGGCGAGCTTT
CCCGCGAAGCCAACGCCAAGGGTGTGTCCCTCGAAGAGCTGCCGATCACCCCGGCCGACGTGGCCGAAGTCGAGAAGCTC
ATCGCCTCCGGCAAGCTCAACGACAAGCTCGCCAAGCAGACCGTCGAAGGCGTGCTGAAGGGCGAAGGCACCCCGGATGA
GGTCGTCAAGAAGCACGACTACAAGATCGTCGAAGACAACGGTGCCATCGAGGCGGCCGTCGACGCGGCGTTCGAGGCGA
ACCCGGATGTCGTCGAGAAGCTCAAGAGCGGCAACATGAAGCCGATGGGCGTGATCATCGGCGCCGTGATGAAGGCCACA
CGCGGCCAAGCCGACGCCAAGGCCGTCACCAAGGTCGTCATGGGCAAGATCAAGGGCTGA

Upstream 100 bases:

>100_bases
GCGCTGGAGGACGGCTGGAACGGCCCGATCTGGAACGACCTCAAGACCCCATGGCTTGACGGTCTGGGCAAGTGAGCATC
GGAAGGAAAGAAACAACATC

Downstream 100 bases:

>100_bases
TTAGATTCACTGGCTCCCGCTGGCGGGAGCTGTCGAACGGAGTAAGACTGAGGGTAGTCGCCATGGTCGGATGACCACCC
TCAGTCCGCTTCGCGGACAG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MAEKLMKYADATKKYDVVFGLETHVELSTNTKLFCPAHIEFGGEPNTELTPVSLGLPGSLPVINKTAVDYAIKLGLALHC
EIAEWSQFARKNYFYPDMPRDYQISQYDKPTNGNGYLDVELEDGTVFRVPIERAHIEDDAGKNTHVGGADGRIEGADHSL
VDYNRAGVPLIEIVTKPIEGAGDRAPEIAGAYVRAIRDIVRALNISHARMEQGNMRADVNVSLRPSPDAPYGTRSETKNV
NSFRGIEKTIQYEIRRQAARLDDGKEILQETRHWDEATQTTAGGRLKSDADDYRYFPDPDLVMLHITKEHIEEMKAQMPE
MPRERRNRLKSEWGLSDLQMRDILNADALDLIEETVKAGAKAAGARKWWLGELSREANAKGVSLEELPITPADVAEVEKL
IASGKLNDKLAKQTVEGVLKGEGTPDEVVKKHDYKIVEDNGAIEAAVDAAFEANPDVVEKLKSGNMKPMGVIIGAVMKAT
RGQADAKAVTKVVMGKIKG

Sequences:

>Translated_499_residues
MAEKLMKYADATKKYDVVFGLETHVELSTNTKLFCPAHIEFGGEPNTELTPVSLGLPGSLPVINKTAVDYAIKLGLALHC
EIAEWSQFARKNYFYPDMPRDYQISQYDKPTNGNGYLDVELEDGTVFRVPIERAHIEDDAGKNTHVGGADGRIEGADHSL
VDYNRAGVPLIEIVTKPIEGAGDRAPEIAGAYVRAIRDIVRALNISHARMEQGNMRADVNVSLRPSPDAPYGTRSETKNV
NSFRGIEKTIQYEIRRQAARLDDGKEILQETRHWDEATQTTAGGRLKSDADDYRYFPDPDLVMLHITKEHIEEMKAQMPE
MPRERRNRLKSEWGLSDLQMRDILNADALDLIEETVKAGAKAAGARKWWLGELSREANAKGVSLEELPITPADVAEVEKL
IASGKLNDKLAKQTVEGVLKGEGTPDEVVKKHDYKIVEDNGAIEAAVDAAFEANPDVVEKLKSGNMKPMGVIIGAVMKAT
RGQADAKAVTKVVMGKIKG
>Mature_498_residues
AEKLMKYADATKKYDVVFGLETHVELSTNTKLFCPAHIEFGGEPNTELTPVSLGLPGSLPVINKTAVDYAIKLGLALHCE
IAEWSQFARKNYFYPDMPRDYQISQYDKPTNGNGYLDVELEDGTVFRVPIERAHIEDDAGKNTHVGGADGRIEGADHSLV
DYNRAGVPLIEIVTKPIEGAGDRAPEIAGAYVRAIRDIVRALNISHARMEQGNMRADVNVSLRPSPDAPYGTRSETKNVN
SFRGIEKTIQYEIRRQAARLDDGKEILQETRHWDEATQTTAGGRLKSDADDYRYFPDPDLVMLHITKEHIEEMKAQMPEM
PRERRNRLKSEWGLSDLQMRDILNADALDLIEETVKAGAKAAGARKWWLGELSREANAKGVSLEELPITPADVAEVEKLI
ASGKLNDKLAKQTVEGVLKGEGTPDEVVKKHDYKIVEDNGAIEAAVDAAFEANPDVVEKLKSGNMKPMGVIIGAVMKATR
GQADAKAVTKVVMGKIKG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=523, Percent_Identity=32.887189292543, Blast_Score=265, Evalue=5e-71,
Organism=Caenorhabditis elegans, GI115534094, Length=322, Percent_Identity=37.2670807453416, Blast_Score=184, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6319391, Length=418, Percent_Identity=30.8612440191388, Blast_Score=167, Evalue=5e-42,
Organism=Drosophila melanogaster, GI45550808, Length=506, Percent_Identity=28.8537549407115, Blast_Score=210, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_BIFLD (B3DU31)

Other databases:

- EMBL:   CP000605
- RefSeq:   YP_001955148.1
- ProteinModelPortal:   B3DU31
- SMR:   B3DU31
- GeneID:   6364274
- GenomeReviews:   CP000605_GR
- KEGG:   blj:BLD_1205
- HOGENOM:   HBG395951
- OMA:   RIHGATH
- ProtClustDB:   PRK05477
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 55058; Mature: 54927

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKLMKYADATKKYDVVFGLETHVELSTNTKLFCPAHIEFGGEPNTELTPVSLGLPGSL
CHHHHHHHHCCCCCEEEEEECEEEEEECCCCEEEEEEEEECCCCCCCCEEEEEECCCCCC
PVINKTAVDYAIKLGLALHCEIAEWSQFARKNYFYPDMPRDYQISQYDKPTNGNGYLDVE
CCCCHHHHHHHHHHCEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
LEDGTVFRVPIERAHIEDDAGKNTHVGGADGRIEGADHSLVDYNRAGVPLIEIVTKPIEG
ECCCEEEEEEHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHCCCCC
AGDRAPEIAGAYVRAIRDIVRALNISHARMEQGNMRADVNVSLRPSPDAPYGTRSETKNV
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCC
NSFRGIEKTIQYEIRRQAARLDDGKEILQETRHWDEATQTTAGGRLKSDADDYRYFPDPD
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
LVMLHITKEHIEEMKAQMPEMPRERRNRLKSEWGLSDLQMRDILNADALDLIEETVKAGA
EEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCH
KAAGARKWWLGELSREANAKGVSLEELPITPADVAEVEKLIASGKLNDKLAKQTVEGVLK
HHCCCCHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
GEGTPDEVVKKHDYKIVEDNGAIEAAVDAAFEANPDVVEKLKSGNMKPMGVIIGAVMKAT
CCCCHHHHHHHCCCEEEECCCCEEHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
RGQADAKAVTKVVMGKIKG
CCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AEKLMKYADATKKYDVVFGLETHVELSTNTKLFCPAHIEFGGEPNTELTPVSLGLPGSL
HHHHHHHHCCCCCEEEEEECEEEEEECCCCEEEEEEEEECCCCCCCCEEEEEECCCCCC
PVINKTAVDYAIKLGLALHCEIAEWSQFARKNYFYPDMPRDYQISQYDKPTNGNGYLDVE
CCCCHHHHHHHHHHCEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
LEDGTVFRVPIERAHIEDDAGKNTHVGGADGRIEGADHSLVDYNRAGVPLIEIVTKPIEG
ECCCEEEEEEHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHCCCCC
AGDRAPEIAGAYVRAIRDIVRALNISHARMEQGNMRADVNVSLRPSPDAPYGTRSETKNV
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCC
NSFRGIEKTIQYEIRRQAARLDDGKEILQETRHWDEATQTTAGGRLKSDADDYRYFPDPD
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
LVMLHITKEHIEEMKAQMPEMPRERRNRLKSEWGLSDLQMRDILNADALDLIEETVKAGA
EEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCH
KAAGARKWWLGELSREANAKGVSLEELPITPADVAEVEKLIASGKLNDKLAKQTVEGVLK
HHCCCCHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
GEGTPDEVVKKHDYKIVEDNGAIEAAVDAAFEANPDVVEKLKSGNMKPMGVIIGAVMKAT
CCCCHHHHHHHCCCEEEECCCCEEHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
RGQADAKAVTKVVMGKIKG
CCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA