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The map label for this gene is gatA [H]

Identifier: 213691179

GI number: 213691179

Start: 303651

End: 305192

Strand: Direct

Name: gatA [H]

Synonym: Blon_0259

Alternate gene names: 213691179

Gene position: 303651-305192 (Clockwise)

Preceding gene: 213691178

Following gene: 213691180

Centisome position: 10.72

GC content: 65.95

Gene sequence:

>1542_bases
ATGAGCAACGAAACCGCAACTCTTGTCAAACTGTCTGCTGCCGAGCAGGCCGCTGCCATCAAGAAGGGCGACGTCACCTC
CCGTGAGCTCGTCGAAGCCCACCTCAAGGTGATCGAAGCCGCTGAGCCGTCTATCAAGGCCTTCCTGAAGGTTTCCGGCG
ATGTGGCTCTCGAGCAGGCCGACGCCTTCGACGCCAAGTCCGCCGAAGACAAGGCCGCCCTGCCTGAGCTGGCTGGTGTG
CCGATCGCCATCAAGGACATGATCGTCACCAAGGGCATCGAAACCACCGCCGCCTCCAAGATCCTCGAGGGCTGGGTGCC
GCCGTACGACGCCACCGTCATCGAAAAGCTCAAGGCCGCCGGCATGCCGATTCTCGGCAAGACCAACCTCGACGAATTCG
CCCAAGGCTCCTCCACCGAGCACTCCGCCTACCAGACCACCCACAACCCGTGGGATACCGAGCGCGTGCCCGGCGGTTCC
GGTGGCGGCTCCGCCTCCGCAGTCGCCGCATTCGAAGCACCGATCGCCCTCGGCACCGACACCGGTGGCTCGATTCGCCA
GCCCGGCGCCCTGACCGGCACCGTGGGCGTCAAACCCACCTACGGCGGCGTCTCCCGTTTCGGAGCCATCGCCATGGCCT
CCTCGCTCGACCAGATCGGCCCGGTCTCCCGCACCGTGCTCGACTCCGCACTGCTGCAGGAGATCATCGGTGGCCACGAC
AAGCGTGACTCCACCTCCATCCCCGAAGGCCCGCGCCCGATGGTCGCCGCCGCCCGCGAAGGCGCCAAGCGCGATCTGAA
GGGCATGAAGGTCGGCCTCATCAAGGAACTCGGCGGCGAGGGCTTCCAGCCCGGCGTCGAGGCCCGTTTCGGCGAGGCCG
TCGACAAGCTCAAGGACATGGGCGCCGAAGTCGTTGAGGTCTCCTGCCCGCACATCGGATACTCGCTGGGCGCCTACTAC
ATCATCATGCCGTCCGAGGTCAGCTCCAACCTGGCCCGTTACGATGGCATGCGCTACGGTCTGCGCGTCATGCCGCCGGC
CGGCGTGCCCCAGACCGCCGCCAACATGATGGCCTACACCCGTGAGGCCGGCTTCGGCGACGAGGTCAAGCGCCGCATCA
TCCTCGGCACCTACGCCCTGTCCGCCGGCTACTATGACGCCTGGTACGGCTCGGCTCAAAAGGTCCGCACGCTCATCATC
GAGGACTTCAAGAAGGCCTTCGAACAGGTCGATGTGCTGATCTCGCCCACCAGCCCGTCCACCGCCTTCAAGTTCGGCGA
GAAGATGGACGATCCGCTGGCCATGTACGTCAACGACATCGCCACGATTCCGGCCAACCTCGCCGGCATGCCCGCCATGT
CCATCCCGGCCGGCCTGTCCGACGACGGCCTGCCCGTCGGCTTCCAGTTCATCGCCCCGCAGCAGCGCGACGAAGTCATG
TACAAGCCGGCTGCGGCCCTCGAAGCAGCGCTGGAGGACGGCTGGAACGGCCCGATCTGGAACGACCTCAAGACCCCATG
GCTTGACGGTCTGGGCAAGTGA

Upstream 100 bases:

>100_bases
CGAAACCCCGCTCACGCAGGAAGAGGCGCTCGCCGGTGGCCCGAAGACCGAGGCCGGCATGTTCGTGGCACCGCGAATCC
TGGGAAGCGAGGAGTAATCG

Downstream 100 bases:

>100_bases
GCATCGGAAGGAAAGAAACAACATCATGGCTGAAAAACTGATGAAGTACGCCGATGCCACCAAGAAGTACGATGTGGTCT
TCGGTCTGGAAACCCACGTG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MSNETATLVKLSAAEQAAAIKKGDVTSRELVEAHLKVIEAAEPSIKAFLKVSGDVALEQADAFDAKSAEDKAALPELAGV
PIAIKDMIVTKGIETTAASKILEGWVPPYDATVIEKLKAAGMPILGKTNLDEFAQGSSTEHSAYQTTHNPWDTERVPGGS
GGGSASAVAAFEAPIALGTDTGGSIRQPGALTGTVGVKPTYGGVSRFGAIAMASSLDQIGPVSRTVLDSALLQEIIGGHD
KRDSTSIPEGPRPMVAAAREGAKRDLKGMKVGLIKELGGEGFQPGVEARFGEAVDKLKDMGAEVVEVSCPHIGYSLGAYY
IIMPSEVSSNLARYDGMRYGLRVMPPAGVPQTAANMMAYTREAGFGDEVKRRIILGTYALSAGYYDAWYGSAQKVRTLII
EDFKKAFEQVDVLISPTSPSTAFKFGEKMDDPLAMYVNDIATIPANLAGMPAMSIPAGLSDDGLPVGFQFIAPQQRDEVM
YKPAAALEAALEDGWNGPIWNDLKTPWLDGLGK

Sequences:

>Translated_513_residues
MSNETATLVKLSAAEQAAAIKKGDVTSRELVEAHLKVIEAAEPSIKAFLKVSGDVALEQADAFDAKSAEDKAALPELAGV
PIAIKDMIVTKGIETTAASKILEGWVPPYDATVIEKLKAAGMPILGKTNLDEFAQGSSTEHSAYQTTHNPWDTERVPGGS
GGGSASAVAAFEAPIALGTDTGGSIRQPGALTGTVGVKPTYGGVSRFGAIAMASSLDQIGPVSRTVLDSALLQEIIGGHD
KRDSTSIPEGPRPMVAAAREGAKRDLKGMKVGLIKELGGEGFQPGVEARFGEAVDKLKDMGAEVVEVSCPHIGYSLGAYY
IIMPSEVSSNLARYDGMRYGLRVMPPAGVPQTAANMMAYTREAGFGDEVKRRIILGTYALSAGYYDAWYGSAQKVRTLII
EDFKKAFEQVDVLISPTSPSTAFKFGEKMDDPLAMYVNDIATIPANLAGMPAMSIPAGLSDDGLPVGFQFIAPQQRDEVM
YKPAAALEAALEDGWNGPIWNDLKTPWLDGLGK
>Mature_512_residues
SNETATLVKLSAAEQAAAIKKGDVTSRELVEAHLKVIEAAEPSIKAFLKVSGDVALEQADAFDAKSAEDKAALPELAGVP
IAIKDMIVTKGIETTAASKILEGWVPPYDATVIEKLKAAGMPILGKTNLDEFAQGSSTEHSAYQTTHNPWDTERVPGGSG
GGSASAVAAFEAPIALGTDTGGSIRQPGALTGTVGVKPTYGGVSRFGAIAMASSLDQIGPVSRTVLDSALLQEIIGGHDK
RDSTSIPEGPRPMVAAAREGAKRDLKGMKVGLIKELGGEGFQPGVEARFGEAVDKLKDMGAEVVEVSCPHIGYSLGAYYI
IMPSEVSSNLARYDGMRYGLRVMPPAGVPQTAANMMAYTREAGFGDEVKRRIILGTYALSAGYYDAWYGSAQKVRTLIIE
DFKKAFEQVDVLISPTSPSTAFKFGEKMDDPLAMYVNDIATIPANLAGMPAMSIPAGLSDDGLPVGFQFIAPQQRDEVMY
KPAAALEAALEDGWNGPIWNDLKTPWLDGLGK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=504, Percent_Identity=39.2857142857143, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI195972892, Length=519, Percent_Identity=25.2408477842004, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17543272, Length=441, Percent_Identity=36.281179138322, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17537465, Length=253, Percent_Identity=31.2252964426877, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=182, Percent_Identity=30.7692307692308, Blast_Score=89, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=252, Percent_Identity=26.984126984127, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=318, Percent_Identity=27.9874213836478, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=320, Percent_Identity=26.5625, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=456, Percent_Identity=36.6228070175439, Blast_Score=246, Evalue=5e-66,
Organism=Saccharomyces cerevisiae, GI6319685, Length=428, Percent_Identity=33.411214953271, Blast_Score=134, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6320448, Length=188, Percent_Identity=27.1276595744681, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24648113, Length=503, Percent_Identity=38.3697813121272, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24644968, Length=529, Percent_Identity=27.9773156899811, Blast_Score=149, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24652985, Length=508, Percent_Identity=26.3779527559055, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI19922090, Length=508, Percent_Identity=26.3779527559055, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24652981, Length=508, Percent_Identity=26.3779527559055, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24652983, Length=508, Percent_Identity=26.3779527559055, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24648435, Length=519, Percent_Identity=25.0481695568401, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24648437, Length=519, Percent_Identity=25.0481695568401, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI45550774, Length=517, Percent_Identity=24.3713733075435, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI161078093, Length=530, Percent_Identity=25.4716981132075, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI21356731, Length=245, Percent_Identity=31.0204081632653, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648441, Length=451, Percent_Identity=24.1685144124169, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648439, Length=451, Percent_Identity=24.1685144124169, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54101; Mature: 53970

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNETATLVKLSAAEQAAAIKKGDVTSRELVEAHLKVIEAAEPSIKAFLKVSGDVALEQA
CCCCCEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEHHH
DAFDAKSAEDKAALPELAGVPIAIKDMIVTKGIETTAASKILEGWVPPYDATVIEKLKAA
CCCCCCCCCHHHCCHHHCCCCEEHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHC
GMPILGKTNLDEFAQGSSTEHSAYQTTHNPWDTERVPGGSGGGSASAVAAFEAPIALGTD
CCCEECCCCHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEECC
TGGSIRQPGALTGTVGVKPTYGGVSRFGAIAMASSLDQIGPVSRTVLDSALLQEIIGGHD
CCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
KRDSTSIPEGPRPMVAAAREGAKRDLKGMKVGLIKELGGEGFQPGVEARFGEAVDKLKDM
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHC
GAEVVEVSCPHIGYSLGAYYIIMPSEVSSNLARYDGMRYGLRVMPPAGVPQTAANMMAYT
CCCEEEECCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHH
REAGFGDEVKRRIILGTYALSAGYYDAWYGSAQKVRTLIIEDFKKAFEQVDVLISPTSPS
HCCCCCHHHHHEEEEEEHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCC
TAFKFGEKMDDPLAMYVNDIATIPANLAGMPAMSIPAGLSDDGLPVGFQFIAPQQRDEVM
HHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHH
YKPAAALEAALEDGWNGPIWNDLKTPWLDGLGK
HCCHHHHHHHHHCCCCCCCCCCCCCHHHCCCCC
>Mature Secondary Structure 
SNETATLVKLSAAEQAAAIKKGDVTSRELVEAHLKVIEAAEPSIKAFLKVSGDVALEQA
CCCCEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEHHH
DAFDAKSAEDKAALPELAGVPIAIKDMIVTKGIETTAASKILEGWVPPYDATVIEKLKAA
CCCCCCCCCHHHCCHHHCCCCEEHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHC
GMPILGKTNLDEFAQGSSTEHSAYQTTHNPWDTERVPGGSGGGSASAVAAFEAPIALGTD
CCCEECCCCHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEECC
TGGSIRQPGALTGTVGVKPTYGGVSRFGAIAMASSLDQIGPVSRTVLDSALLQEIIGGHD
CCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
KRDSTSIPEGPRPMVAAAREGAKRDLKGMKVGLIKELGGEGFQPGVEARFGEAVDKLKDM
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHC
GAEVVEVSCPHIGYSLGAYYIIMPSEVSSNLARYDGMRYGLRVMPPAGVPQTAANMMAYT
CCCEEEECCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHH
REAGFGDEVKRRIILGTYALSAGYYDAWYGSAQKVRTLIIEDFKKAFEQVDVLISPTSPS
HCCCCCHHHHHEEEEEEHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCC
TAFKFGEKMDDPLAMYVNDIATIPANLAGMPAMSIPAGLSDDGLPVGFQFIAPQQRDEVM
HHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHH
YKPAAALEAALEDGWNGPIWNDLKTPWLDGLGK
HCCHHHHHHHHHCCCCCCCCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA