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The map label for this gene is gla [H]

Identifier: 213691170

GI number: 213691170

Start: 294287

End: 295021

Strand: Direct

Name: gla [H]

Synonym: Blon_0250

Alternate gene names: 213691170

Gene position: 294287-295021 (Clockwise)

Preceding gene: 213691168

Following gene: 213691172

Centisome position: 10.39

GC content: 62.18

Gene sequence:

>735_bases
GTGGATTATCCTCTCTTTACCAAGCTGGCCGCCGAGTTCTTCGGCACCGCCATTCTGATGATCTTCGGCAACGGCTCCGT
GGCCAACGTCGAGCTCAAGAACACCAAGGGCCACCACGCCGGCTGGCTGAACATCGCCATGGGCTATGGCTTCGGCGTCA
TGTTCCCGGTGCTCATGTTCGGTGGCGTATCCGGCGCTCATATCAACCCGGCCATGACCCTTGCTCAGGCCATCAACGGC
CTGTTCCCCTGGAATGAAGTGCTGCCGTACATCGTCGCCCAGCTGCTCGGCGCCCTGCTTGGCCAGCTCATCGTCTACAT
CACTTACTACCCGCACTACCAGGAGACCGAGGACGCCGAGGCGATTCTGGGCACCTTCTGCACCACTGACGCGGACAACA
AGAAGGTCAACTACTTCCTCAACGAGATGTTCGGCACGCTCGTGCTGGTGTTCGGCGCACTGTGCTGCCTGACGCTGCCG
TGGGGCAAGGCCGACGACGCCGCATCCTCCATCGTGGTCGGCTTCATTGTGTGGGGCCTCGTGACCTCCATGGGTGGCCC
GACCGGCCCTGGCCTGAACCCGGCCCGTGATCTCATGCCGCGACTGCTGCACGCCATCCTGCCGATTCCGAACAAGGGTA
CGTCCCGCTGGGGCGAAGCCTGGATCCCGGTTGTGGCCCCGATTGTCGGTGCCATTATCGGTGTCTGGCTGTACGTGTTC
TTCTTCGCCAGCTGA

Upstream 100 bases:

>100_bases
GTAAATCGCGTCGCCGGCTCCGCGAACGGATTTCCAATCACACAGAATGCCGTGAACAGTCAGCCATGCCTGTTGCGCGG
CGAAGAGAAAAGAAGAAGCA

Downstream 100 bases:

>100_bases
TTCGTTGATTGATTCATGCATGCAAATGGCCTCCGCTCGTTTTGGGCGGAGGCCATTGTCGTTGAATCAGGTAAACGCAG
AGACTACTGCTCCAGGAACA

Product: MIP family channel protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Aquaglyceroporin; Glyceroaquaporin [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MDYPLFTKLAAEFFGTAILMIFGNGSVANVELKNTKGHHAGWLNIAMGYGFGVMFPVLMFGGVSGAHINPAMTLAQAING
LFPWNEVLPYIVAQLLGALLGQLIVYITYYPHYQETEDAEAILGTFCTTDADNKKVNYFLNEMFGTLVLVFGALCCLTLP
WGKADDAASSIVVGFIVWGLVTSMGGPTGPGLNPARDLMPRLLHAILPIPNKGTSRWGEAWIPVVAPIVGAIIGVWLYVF
FFAS

Sequences:

>Translated_244_residues
MDYPLFTKLAAEFFGTAILMIFGNGSVANVELKNTKGHHAGWLNIAMGYGFGVMFPVLMFGGVSGAHINPAMTLAQAING
LFPWNEVLPYIVAQLLGALLGQLIVYITYYPHYQETEDAEAILGTFCTTDADNKKVNYFLNEMFGTLVLVFGALCCLTLP
WGKADDAASSIVVGFIVWGLVTSMGGPTGPGLNPARDLMPRLLHAILPIPNKGTSRWGEAWIPVVAPIVGAIIGVWLYVF
FFAS
>Mature_244_residues
MDYPLFTKLAAEFFGTAILMIFGNGSVANVELKNTKGHHAGWLNIAMGYGFGVMFPVLMFGGVSGAHINPAMTLAQAING
LFPWNEVLPYIVAQLLGALLGQLIVYITYYPHYQETEDAEAILGTFCTTDADNKKVNYFLNEMFGTLVLVFGALCCLTLP
WGKADDAASSIVVGFIVWGLVTSMGGPTGPGLNPARDLMPRLLHAILPIPNKGTSRWGEAWIPVVAPIVGAIIGVWLYVF
FFAS

Specific function: Mixed channel protein that transports both water and glycerol [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=255, Percent_Identity=34.9019607843137, Blast_Score=126, Evalue=1e-29,
Organism=Homo sapiens, GI157266307, Length=254, Percent_Identity=32.2834645669291, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI4502187, Length=259, Percent_Identity=31.6602316602317, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI22538420, Length=252, Percent_Identity=32.9365079365079, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI310133356, Length=214, Percent_Identity=29.4392523364486, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI310114181, Length=214, Percent_Identity=29.4392523364486, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI4502179, Length=233, Percent_Identity=30.4721030042918, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1790362, Length=244, Percent_Identity=31.9672131147541, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71994009, Length=246, Percent_Identity=33.3333333333333, Blast_Score=115, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71992966, Length=249, Percent_Identity=31.3253012048193, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17558372, Length=250, Percent_Identity=29.6, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17533613, Length=245, Percent_Identity=31.0204081632653, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI32564052, Length=245, Percent_Identity=31.0204081632653, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17544068, Length=259, Percent_Identity=29.7297297297297, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71992961, Length=248, Percent_Identity=29.0322580645161, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17531431, Length=244, Percent_Identity=29.0983606557377, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17531429, Length=244, Percent_Identity=29.0983606557377, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71993722, Length=241, Percent_Identity=24.896265560166, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=203, Percent_Identity=34.4827586206897, Blast_Score=104, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322985, Length=273, Percent_Identity=27.4725274725275, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI45550503, Length=241, Percent_Identity=27.3858921161826, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 26338; Mature: 26338

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYPLFTKLAAEFFGTAILMIFGNGSVANVELKNTKGHHAGWLNIAMGYGFGVMFPVLMF
CCCCHHHHHHHHHHHHEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
GGVSGAHINPAMTLAQAINGLFPWNEVLPYIVAQLLGALLGQLIVYITYYPHYQETEDAE
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
AILGTFCTTDADNKKVNYFLNEMFGTLVLVFGALCCLTLPWGKADDAASSIVVGFIVWGL
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
VTSMGGPTGPGLNPARDLMPRLLHAILPIPNKGTSRWGEAWIPVVAPIVGAIIGVWLYVF
HHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FFAS
HHCC
>Mature Secondary Structure
MDYPLFTKLAAEFFGTAILMIFGNGSVANVELKNTKGHHAGWLNIAMGYGFGVMFPVLMF
CCCCHHHHHHHHHHHHEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
GGVSGAHINPAMTLAQAINGLFPWNEVLPYIVAQLLGALLGQLIVYITYYPHYQETEDAE
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
AILGTFCTTDADNKKVNYFLNEMFGTLVLVFGALCCLTLPWGKADDAASSIVVGFIVWGL
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
VTSMGGPTGPGLNPARDLMPRLLHAILPIPNKGTSRWGEAWIPVVAPIVGAIIGVWLYVF
HHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FFAS
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]