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The map label for this gene is dnaB [H]

Identifier: 213691148

GI number: 213691148

Start: 272640

End: 274100

Strand: Direct

Name: dnaB [H]

Synonym: Blon_0227

Alternate gene names: 213691148

Gene position: 272640-274100 (Clockwise)

Preceding gene: 213691146

Following gene: 213691149

Centisome position: 9.62

GC content: 61.67

Gene sequence:

>1461_bases
ATGGCAGAAGGAAGTGAACACTCGTATCAGAATGGGTTCGGCGGTGGCTTGGATCGTGGCGATCGTGGCGACCGCGCGGG
CCGGGGCGGCGCACAGACGTCGGCGGCGGCCGGCGACCCGATTTTCGACCGTGTGCCCCCGCATGATGACGACGCGGAAA
TGGCCGTGCTCGGCGGCATGCTGATGAGTAAGGATGCCATCGGCGAGGTCTCGCAGACCATTGACGTCACCGATTTCTAC
CAGCCCAAGCATCAGACGATCTATAACGCGATCATCGATCTGTTTTCCGCCAGTCAGCCGGTGGACGTGGTGCTCGTGGC
CAACCAGCTGTTGGCGGATGGCAACCTCGACAAGGTCGGCGGCGCGGACTATCTGCACAGCCTCGTCGCATCCGTACCGA
CCGCCGCCAATGCCATGTACTACGCGGAAATCGTCCATCAGCGGGCCATTTTGCGCAATGTGATCGCCGCCGGCACCAAA
ATCGCCCAGCTCGGTTATTCCGCCGAGGGGTCGCAGGCCGAGGACGTGGTTAACCTCGCCCAGTCCGAGATCTACGAGAT
GTCGATGGGCAAGGTGCGTCAGGACTATGCCGCCATCGGCCCGGTGGTCCACGACGCGCTCGACCAGCTGGACAAACTGC
AGCAGGGTCTCGTCAACAAGGGCGTGCCGACCGGATTCCGCGACATCGACGACGTGACCCAGGGCCTGCAGCCCGGACAG
ATGGTCGTCGTCGCAGGACGTCCGGCCATGGGTAAGTCCACACTCGGCATCGACTTCGCCCGAGCCGCCGCGTTGCACCA
CAACATGACTTCCGTGGTCTTCAGCTTGGAGATGAGCAAGGTGGAACTCGCCCAGCGCATCATTTCCGCCGAAACCAATA
TTCCGATGGCCGCCCTGCGCCGCGCCGACGACATCACCCCCGAACGCTGGAACACCCTGAACCAGTTCTGGACCAAGATG
CAGAACGCGCCGCTGTTCATTGACGACAGCCCGAACATGAGCCTCATGGAGATCCGCGCGAAATGCCGTCGCCTGAAGCA
GACCAACGATCTGAAGCTCGTGGTCATCGACTACCTGCAGCTCATGACCTCGGGCAAGGCCGTGGAATCCCGCCAGCAAG
AGGTATCCGAATTCTCCCGTGCCCTGAAGCTCTTGGCCAAGGAACTCGAAGTGCCGGTTGTGGCCTTGTCCCAGCTGAAC
CGTGGCCCGGAAATGCGTAACGACAAGAAGCCGCAGCTCTCCGATTTGCGTGAATCCGGCTCAATCGAACAGGACGCCGA
CGTGGTGTTCCTGGTCCACCGCCCTGACTTCTACGACAAGGAAGACCGCCCCGGCGAGGCCGATATCATCATGGCCAAGC
ACCGTAACGGCCCGACCGAAACCTTCCACCTCGCCTTCCTCGGTGCCACGTCCAAATTCAAGGACATGCCGCAGGACTAC
AACAGCAATCAGGGGGTGTGA

Upstream 100 bases:

>100_bases
GCTGACTCATAGGTGAATGAGAAATGAACCGATGCGGGCAGCCGTTTCGTTTCCACGCGATATCGTTCACCCGCCACGCC
ACATCCTATACTTGAAAGCT

Downstream 100 bases:

>100_bases
TGATGGCAAGCAACGTTTCACGCGATGTTTCGCAAGACCAATCGTCCGTGGCACAGGCCCGCAAACCGTGGTATGCGTTC
GCCACCGTGGCCGCAGGCCG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MAEGSEHSYQNGFGGGLDRGDRGDRAGRGGAQTSAAAGDPIFDRVPPHDDDAEMAVLGGMLMSKDAIGEVSQTIDVTDFY
QPKHQTIYNAIIDLFSASQPVDVVLVANQLLADGNLDKVGGADYLHSLVASVPTAANAMYYAEIVHQRAILRNVIAAGTK
IAQLGYSAEGSQAEDVVNLAQSEIYEMSMGKVRQDYAAIGPVVHDALDQLDKLQQGLVNKGVPTGFRDIDDVTQGLQPGQ
MVVVAGRPAMGKSTLGIDFARAAALHHNMTSVVFSLEMSKVELAQRIISAETNIPMAALRRADDITPERWNTLNQFWTKM
QNAPLFIDDSPNMSLMEIRAKCRRLKQTNDLKLVVIDYLQLMTSGKAVESRQQEVSEFSRALKLLAKELEVPVVALSQLN
RGPEMRNDKKPQLSDLRESGSIEQDADVVFLVHRPDFYDKEDRPGEADIIMAKHRNGPTETFHLAFLGATSKFKDMPQDY
NSNQGV

Sequences:

>Translated_486_residues
MAEGSEHSYQNGFGGGLDRGDRGDRAGRGGAQTSAAAGDPIFDRVPPHDDDAEMAVLGGMLMSKDAIGEVSQTIDVTDFY
QPKHQTIYNAIIDLFSASQPVDVVLVANQLLADGNLDKVGGADYLHSLVASVPTAANAMYYAEIVHQRAILRNVIAAGTK
IAQLGYSAEGSQAEDVVNLAQSEIYEMSMGKVRQDYAAIGPVVHDALDQLDKLQQGLVNKGVPTGFRDIDDVTQGLQPGQ
MVVVAGRPAMGKSTLGIDFARAAALHHNMTSVVFSLEMSKVELAQRIISAETNIPMAALRRADDITPERWNTLNQFWTKM
QNAPLFIDDSPNMSLMEIRAKCRRLKQTNDLKLVVIDYLQLMTSGKAVESRQQEVSEFSRALKLLAKELEVPVVALSQLN
RGPEMRNDKKPQLSDLRESGSIEQDADVVFLVHRPDFYDKEDRPGEADIIMAKHRNGPTETFHLAFLGATSKFKDMPQDY
NSNQGV
>Mature_485_residues
AEGSEHSYQNGFGGGLDRGDRGDRAGRGGAQTSAAAGDPIFDRVPPHDDDAEMAVLGGMLMSKDAIGEVSQTIDVTDFYQ
PKHQTIYNAIIDLFSASQPVDVVLVANQLLADGNLDKVGGADYLHSLVASVPTAANAMYYAEIVHQRAILRNVIAAGTKI
AQLGYSAEGSQAEDVVNLAQSEIYEMSMGKVRQDYAAIGPVVHDALDQLDKLQQGLVNKGVPTGFRDIDDVTQGLQPGQM
VVVAGRPAMGKSTLGIDFARAAALHHNMTSVVFSLEMSKVELAQRIISAETNIPMAALRRADDITPERWNTLNQFWTKMQ
NAPLFIDDSPNMSLMEIRAKCRRLKQTNDLKLVVIDYLQLMTSGKAVESRQQEVSEFSRALKLLAKELEVPVVALSQLNR
GPEMRNDKKPQLSDLRESGSIEQDADVVFLVHRPDFYDKEDRPGEADIIMAKHRNGPTETFHLAFLGATSKFKDMPQDYN
SNQGV

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity [H]

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=439, Percent_Identity=36.9020501138952, Blast_Score=299, Evalue=2e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 53159; Mature: 53028

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEGSEHSYQNGFGGGLDRGDRGDRAGRGGAQTSAAAGDPIFDRVPPHDDDAEMAVLGGM
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LMSKDAIGEVSQTIDVTDFYQPKHQTIYNAIIDLFSASQPVDVVLVANQLLADGNLDKVG
HHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCCCCCCC
GADYLHSLVASVPTAANAMYYAEIVHQRAILRNVIAAGTKIAQLGYSAEGSQAEDVVNLA
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHH
QSEIYEMSMGKVRQDYAAIGPVVHDALDQLDKLQQGLVNKGVPTGFRDIDDVTQGLQPGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCC
MVVVAGRPAMGKSTLGIDFARAAALHHNMTSVVFSLEMSKVELAQRIISAETNIPMAALR
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RADDITPERWNTLNQFWTKMQNAPLFIDDSPNMSLMEIRAKCRRLKQTNDLKLVVIDYLQ
HHCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHH
LMTSGKAVESRQQEVSEFSRALKLLAKELEVPVVALSQLNRGPEMRNDKKPQLSDLRESG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCHHHHHHCC
SIEQDADVVFLVHRPDFYDKEDRPGEADIIMAKHRNGPTETFHLAFLGATSKFKDMPQDY
CCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCHHHHCCCCCC
NSNQGV
CCCCCC
>Mature Secondary Structure 
AEGSEHSYQNGFGGGLDRGDRGDRAGRGGAQTSAAAGDPIFDRVPPHDDDAEMAVLGGM
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH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CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA