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The map label for this gene is gspE [C]

Identifier: 213691073

GI number: 213691073

Start: 181643

End: 182710

Strand: Direct

Name: gspE [C]

Synonym: Blon_0152

Alternate gene names: 213691073

Gene position: 181643-182710 (Clockwise)

Preceding gene: 213691072

Following gene: 213691074

Centisome position: 6.41

GC content: 61.14

Gene sequence:

>1068_bases
ATGGCTGCCGTAATCACTCGGGCTGATGAGCCCGGCGCAGTGCCCAGAACTGTGTTCTTCGGTCTATTGAATGATGTGGC
ATCCGACCCGCAGGTTACCGATTTGGCGGTGACCTGCGATGGCCGCGTTTGGGCGGACCGCGGCAATGGCATGCGCGAAA
CAGTATTGCATGTGCCGTTCCGCTCGCCGCAAGTAGTTCGAGAATACGCCGTCCAACTGTGCGCGCAGCTTGGGCGCAGA
CTGGATGATGCCTGCCCGATAGCGGACGCCGCCAGTCCAGATGGCATCCGCGTGCATGCGGTGATTGCCCCATTGGTGCC
ACAAGGTGCCGCGATTTCCATACGATTCCCTGACCGAACCATGGCATCGCTACAGCACCTGAGCAAATTAGGCGTATTCC
CGGCTGCTTGGCTGCTGACGTTCGTGGGATTGGTGCGCAAACATGCCACAGTGCTAATTACGGGCGGAACAGGCGTGGGC
AAGACCACCTTGCTCAAGGCGCTGCTGGCGCAATGCGACGTGAACGAGCGCATCGTCACCGTAGAGGAAGTTCGTGAACT
GGGGGAACTGGGACGCGGCGATCATGTCTCGTTGGTAACACGCGAGGCGAATGTGGAGGGCGTCGGAGCCATAAGCCTGG
CGGACTTGGTCAAAGCGACATTGCGAATGCGCCCCGACCGCGTGGTGCTGGGCGAATGCCGTGGTGCGGAAATCGTTGAC
CTACTGCGCGCGTTCAATTCCGGTCACCACGGCGGCATGACCACCGTGCATGCGGACGGCGTGGACCGAGTGCCGTCCAG
GTTGATTTCGCTTGGATTGCTTGCCGGAGTCAGTCCACAGGCGTTGGCCATGCTGGCGGCAGGTGCTTTCGACGCGGTGG
TGCATTTGGAGCGAGTGAACGGACAGCGGCGTATCACGCAGATCGGCGAACTGCGCGTAGCACATGGTGAACTGGCAGGA
ACACCGCTGGCGGTATGGAATGGCCGCCATCCTCCTCGGTATGCCGCCGAATGGGGGCACTTCATTGAACGATGGGGCAT
CGTTCCCGGGCCGAATGGTGGTGGGTGA

Upstream 100 bases:

>100_bases
GTGACCAAAGGCAGTCGCAAAGCCATGAACTTGCTTGCCGATCTCGTAGAACAGGCCATTCGCTCGGTTTCGGGAACTTC
TCACAAGGACCAGTGATGCT

Downstream 100 bases:

>100_bases
TGATGTGGTGTGCTGTTCTGGCGGCGCTCGCGGTGCTGATATGGCCGGGGAAGAAGTCCGGAAGATTGCGCCAGCTGGCT
GGTAAAGAGCGCACGGTCGA

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MAAVITRADEPGAVPRTVFFGLLNDVASDPQVTDLAVTCDGRVWADRGNGMRETVLHVPFRSPQVVREYAVQLCAQLGRR
LDDACPIADAASPDGIRVHAVIAPLVPQGAAISIRFPDRTMASLQHLSKLGVFPAAWLLTFVGLVRKHATVLITGGTGVG
KTTLLKALLAQCDVNERIVTVEEVRELGELGRGDHVSLVTREANVEGVGAISLADLVKATLRMRPDRVVLGECRGAEIVD
LLRAFNSGHHGGMTTVHADGVDRVPSRLISLGLLAGVSPQALAMLAAGAFDAVVHLERVNGQRRITQIGELRVAHGELAG
TPLAVWNGRHPPRYAAEWGHFIERWGIVPGPNGGG

Sequences:

>Translated_355_residues
MAAVITRADEPGAVPRTVFFGLLNDVASDPQVTDLAVTCDGRVWADRGNGMRETVLHVPFRSPQVVREYAVQLCAQLGRR
LDDACPIADAASPDGIRVHAVIAPLVPQGAAISIRFPDRTMASLQHLSKLGVFPAAWLLTFVGLVRKHATVLITGGTGVG
KTTLLKALLAQCDVNERIVTVEEVRELGELGRGDHVSLVTREANVEGVGAISLADLVKATLRMRPDRVVLGECRGAEIVD
LLRAFNSGHHGGMTTVHADGVDRVPSRLISLGLLAGVSPQALAMLAAGAFDAVVHLERVNGQRRITQIGELRVAHGELAG
TPLAVWNGRHPPRYAAEWGHFIERWGIVPGPNGGG
>Mature_354_residues
AAVITRADEPGAVPRTVFFGLLNDVASDPQVTDLAVTCDGRVWADRGNGMRETVLHVPFRSPQVVREYAVQLCAQLGRRL
DDACPIADAASPDGIRVHAVIAPLVPQGAAISIRFPDRTMASLQHLSKLGVFPAAWLLTFVGLVRKHATVLITGGTGVGK
TTLLKALLAQCDVNERIVTVEEVRELGELGRGDHVSLVTREANVEGVGAISLADLVKATLRMRPDRVVLGECRGAEIVDL
LRAFNSGHHGGMTTVHADGVDRVPSRLISLGLLAGVSPQALAMLAAGAFDAVVHLERVNGQRRITQIGELRVAHGELAGT
PLAVWNGRHPPRYAAEWGHFIERWGIVPGPNGGG

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 37790; Mature: 37659

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVITRADEPGAVPRTVFFGLLNDVASDPQVTDLAVTCDGRVWADRGNGMRETVLHVPF
CCEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCEEEECCCCCHHHHEEECCC
RSPQVVREYAVQLCAQLGRRLDDACPIADAASPDGIRVHAVIAPLVPQGAAISIRFPDRT
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCHH
MASLQHLSKLGVFPAAWLLTFVGLVRKHATVLITGGTGVGKTTLLKALLAQCDVNERIVT
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCEEE
VEEVRELGELGRGDHVSLVTREANVEGVGAISLADLVKATLRMRPDRVVLGECRGAEIVD
HHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHH
LLRAFNSGHHGGMTTVHADGVDRVPSRLISLGLLAGVSPQALAMLAAGAFDAVVHLERVN
HHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
GQRRITQIGELRVAHGELAGTPLAVWNGRHPPRYAAEWGHFIERWGIVPGPNGGG
CHHHHHHHHHEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AAVITRADEPGAVPRTVFFGLLNDVASDPQVTDLAVTCDGRVWADRGNGMRETVLHVPF
CEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCEEEECCCCCHHHHEEECCC
RSPQVVREYAVQLCAQLGRRLDDACPIADAASPDGIRVHAVIAPLVPQGAAISIRFPDRT
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCHH
MASLQHLSKLGVFPAAWLLTFVGLVRKHATVLITGGTGVGKTTLLKALLAQCDVNERIVT
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCEEE
VEEVRELGELGRGDHVSLVTREANVEGVGAISLADLVKATLRMRPDRVVLGECRGAEIVD
HHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHH
LLRAFNSGHHGGMTTVHADGVDRVPSRLISLGLLAGVSPQALAMLAAGAFDAVVHLERVN
HHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
GQRRITQIGELRVAHGELAGTPLAVWNGRHPPRYAAEWGHFIERWGIVPGPNGGG
CHHHHHHHHHEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]