Click here to switch to the map view.

The map label for this gene is clcA [H]

Identifier: 213691045

GI number: 213691045

Start: 145420

End: 146934

Strand: Direct

Name: clcA [H]

Synonym: Blon_0124

Alternate gene names: 213691045

Gene position: 145420-146934 (Clockwise)

Preceding gene: 213691044

Following gene: 213691046

Centisome position: 5.13

GC content: 61.98

Gene sequence:

>1515_bases
ATGGCGGCGGCCGGCATAGTCATTGGTCTGGTTTCAGGATTGCTGGTTGTCGTATACCGTCTCGGCATCGAATACGGCAC
GGATGCGTCCCGTTGGATATATGCGCGGATTCGCGAGACCCCGTGGCTGATTGCGCCGTGGGTGGTTGCCGCGGTGGCGG
CCGCGCTGGCGATCGCGTGGATGGTCGGCAAGGAGCCGATGGCTGGTGGCAGCGGCATCCCGCAGACCAACGGCGTGGTG
ATATGCGGCTTGAAGATGCGTTGGCAGACGATTCTGCCAGTCCGTTTCGTCGGTGGACTGTTGGGCGCGTTGTTTGGTCT
GTCGCTCGGCCGTGAGGGGCCGTCCATTCAGATTGGTGCGTCGGGCGCGCAGTGTGTGTCCCACCGTTTGCGCGGCCGCC
GCCGTGAGGACATGCAGGAGCATTATCTGGTCACCGCCGGTGCCGCGGCCGGCTTGTCCGCCGCGTTCAGCGCGCCGCTG
TCCGGCATGATGTTCGCTTTGGAAGGGGTGCATCGGAGTTTTTCCCCTGCGATTCTGATGGGCGCCACCGCCGCATCGCT
GACCGCCGATTTCGTCTCAAAATACTGTTTCGGTCTGCGTCCGGTTTTGGATTTCGGTGATATCGGTCAGCTGTCGTTGG
AAGAGTATGTGTGGCTGATTCCGTTGGGATTGGTGGCCGGTTTGGTTGGTTCGCTGATGAACCGCTCGTTGCTTGGCTTC
CAGACGCTGTACGACAAACTGCCGGCATGGAGCCGCCCGATGATTGCGATTGCGATCGCGTTGCCTGTCGGCATCTGGTT
GCCCGATGTGCTCGGCGGCGGCTCGAATCTGATTGATACCGCCGAACATGCGCGCGTCGGCTTGGGAATGCTGTGTCTGC
TGTTCGTGGCGAAGATGCTGTTCACATCCACGAGTTTCGGCTCAGGCGCGCCTGGCGGTATTTTCATGCCGATTCTTGCG
GTCGGTTCGTTGGCCGGCGGCATCTGCGGTGAAGTGTTGCATCGATTCGGTAATCTGCCATCCGACGCAGTGGCGGTGTT
TGCGGTGTGCGTGATGACGGGCACGCTCGCCGCGTCCGTGAAAACGCCGATCACGTCGATTCTGTTGGCTGTGGAGATGT
CTGGAACGTTGACGCACATGCTGCCGGTCGCGGCCGTCGCTTTCATCGCGCTGTTGGTGTCCGATTTGCTGCGCACCAAA
CCGATCTATGGGGAGTTGCTGGAGCGGTACATGCGCGCGCAAGGCATGCAGATGGCGATCGCAAGCCATGTGGGCAGCGG
CATTATGGAACTGCCATTGGAAATGGGAGCGATTGCGGACGGCAAGCGGGTGCGTGACGTGCGTTGGCCGTCTGGGTGCC
TGATCATCGGCCTGCGTCGCGGCGAAAGCGAGATCGTGCCGCGCGGCGATACACGGCTGCGTGCCGGCGACTATCTGGTG
GTGCTGTTCAGTGGCGAGGAGGAGCGCGAGGTGCGAACTGCGATGCGGCGGCTGTGTGATGCCCGGCTTGACTGA

Upstream 100 bases:

>100_bases
TCGACTGACCGTCTGCAACGAATTTCTCGGCTTGACTGGTAACGACGCGGCACATGATATCCGCCGGCTGATTCCGCGAT
TCGACCACTTGAAGTGGAAG

Downstream 100 bases:

>100_bases
GCCTGGCTGAATTCGGCTGGACTCTGGATGTCCGAATCTGCGGAATCGGACATCCAGGGTGCATACGTTGGCGCTCCGTG
TAAACTCATATGCGTTAGGA

Product: Cl- channel, voltage-gated family protein

Products: Cl Ion [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MAAAGIVIGLVSGLLVVVYRLGIEYGTDASRWIYARIRETPWLIAPWVVAAVAAALAIAWMVGKEPMAGGSGIPQTNGVV
ICGLKMRWQTILPVRFVGGLLGALFGLSLGREGPSIQIGASGAQCVSHRLRGRRREDMQEHYLVTAGAAAGLSAAFSAPL
SGMMFALEGVHRSFSPAILMGATAASLTADFVSKYCFGLRPVLDFGDIGQLSLEEYVWLIPLGLVAGLVGSLMNRSLLGF
QTLYDKLPAWSRPMIAIAIALPVGIWLPDVLGGGSNLIDTAEHARVGLGMLCLLFVAKMLFTSTSFGSGAPGGIFMPILA
VGSLAGGICGEVLHRFGNLPSDAVAVFAVCVMTGTLAASVKTPITSILLAVEMSGTLTHMLPVAAVAFIALLVSDLLRTK
PIYGELLERYMRAQGMQMAIASHVGSGIMELPLEMGAIADGKRVRDVRWPSGCLIIGLRRGESEIVPRGDTRLRAGDYLV
VLFSGEEEREVRTAMRRLCDARLD

Sequences:

>Translated_504_residues
MAAAGIVIGLVSGLLVVVYRLGIEYGTDASRWIYARIRETPWLIAPWVVAAVAAALAIAWMVGKEPMAGGSGIPQTNGVV
ICGLKMRWQTILPVRFVGGLLGALFGLSLGREGPSIQIGASGAQCVSHRLRGRRREDMQEHYLVTAGAAAGLSAAFSAPL
SGMMFALEGVHRSFSPAILMGATAASLTADFVSKYCFGLRPVLDFGDIGQLSLEEYVWLIPLGLVAGLVGSLMNRSLLGF
QTLYDKLPAWSRPMIAIAIALPVGIWLPDVLGGGSNLIDTAEHARVGLGMLCLLFVAKMLFTSTSFGSGAPGGIFMPILA
VGSLAGGICGEVLHRFGNLPSDAVAVFAVCVMTGTLAASVKTPITSILLAVEMSGTLTHMLPVAAVAFIALLVSDLLRTK
PIYGELLERYMRAQGMQMAIASHVGSGIMELPLEMGAIADGKRVRDVRWPSGCLIIGLRRGESEIVPRGDTRLRAGDYLV
VLFSGEEEREVRTAMRRLCDARLD
>Mature_503_residues
AAAGIVIGLVSGLLVVVYRLGIEYGTDASRWIYARIRETPWLIAPWVVAAVAAALAIAWMVGKEPMAGGSGIPQTNGVVI
CGLKMRWQTILPVRFVGGLLGALFGLSLGREGPSIQIGASGAQCVSHRLRGRRREDMQEHYLVTAGAAAGLSAAFSAPLS
GMMFALEGVHRSFSPAILMGATAASLTADFVSKYCFGLRPVLDFGDIGQLSLEEYVWLIPLGLVAGLVGSLMNRSLLGFQ
TLYDKLPAWSRPMIAIAIALPVGIWLPDVLGGGSNLIDTAEHARVGLGMLCLLFVAKMLFTSTSFGSGAPGGIFMPILAV
GSLAGGICGEVLHRFGNLPSDAVAVFAVCVMTGTLAASVKTPITSILLAVEMSGTLTHMLPVAAVAFIALLVSDLLRTKP
IYGELLERYMRAQGMQMAIASHVGSGIMELPLEMGAIADGKRVRDVRWPSGCLIIGLRRGESEIVPRGDTRLRAGDYLVV
LFSGEEEREVRTAMRRLCDARLD

Specific function: Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557473, Length=420, Percent_Identity=24.7619047619048, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI189217923, Length=420, Percent_Identity=24.7619047619048, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI189217921, Length=420, Percent_Identity=24.7619047619048, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI55770838, Length=429, Percent_Identity=24.2424242424242, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI55770840, Length=429, Percent_Identity=24.2424242424242, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI153252026, Length=423, Percent_Identity=25.0591016548463, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1786350, Length=437, Percent_Identity=31.5789473684211, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI87081943, Length=326, Percent_Identity=29.7546012269939, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17532615, Length=467, Percent_Identity=23.7687366167024, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24665008, Length=432, Percent_Identity=25.6944444444444, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI45551566, Length=418, Percent_Identity=26.0765550239234, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 53396; Mature: 53265

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAAGIVIGLVSGLLVVVYRLGIEYGTDASRWIYARIRETPWLIAPWVVAAVAAALAIAW
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHEECCCCCCCHHHHHHHHHHHHHHHHH
MVGKEPMAGGSGIPQTNGVVICGLKMRWQTILPVRFVGGLLGALFGLSLGREGPSIQIGA
HHCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
SGAQCVSHRLRGRRREDMQEHYLVTAGAAAGLSAAFSAPLSGMMFALEGVHRSFSPAILM
CHHHHHHHHHCCCHHHHHHHHEEEECCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHC
GATAASLTADFVSKYCFGLRPVLDFGDIGQLSLEEYVWLIPLGLVAGLVGSLMNRSLLGF
CCHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTLYDKLPAWSRPMIAIAIALPVGIWLPDVLGGGSNLIDTAEHARVGLGMLCLLFVAKML
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FTSTSFGSGAPGGIFMPILAVGSLAGGICGEVLHRFGNLPSDAVAVFAVCVMTGTLAASV
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
KTPITSILLAVEMSGTLTHMLPVAAVAFIALLVSDLLRTKPIYGELLERYMRAQGMQMAI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHH
ASHVGSGIMELPLEMGAIADGKRVRDVRWPSGCLIIGLRRGESEIVPRGDTRLRAGDYLV
HHHHCCCHHHCCHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEECCCEEE
VLFSGEEEREVRTAMRRLCDARLD
EEECCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AAAGIVIGLVSGLLVVVYRLGIEYGTDASRWIYARIRETPWLIAPWVVAAVAAALAIAW
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHEECCCCCCCHHHHHHHHHHHHHHHHH
MVGKEPMAGGSGIPQTNGVVICGLKMRWQTILPVRFVGGLLGALFGLSLGREGPSIQIGA
HHCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
SGAQCVSHRLRGRRREDMQEHYLVTAGAAAGLSAAFSAPLSGMMFALEGVHRSFSPAILM
CHHHHHHHHHCCCHHHHHHHHEEEECCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHC
GATAASLTADFVSKYCFGLRPVLDFGDIGQLSLEEYVWLIPLGLVAGLVGSLMNRSLLGF
CCHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTLYDKLPAWSRPMIAIAIALPVGIWLPDVLGGGSNLIDTAEHARVGLGMLCLLFVAKML
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FTSTSFGSGAPGGIFMPILAVGSLAGGICGEVLHRFGNLPSDAVAVFAVCVMTGTLAASV
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
KTPITSILLAVEMSGTLTHMLPVAAVAFIALLVSDLLRTKPIYGELLERYMRAQGMQMAI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHH
ASHVGSGIMELPLEMGAIADGKRVRDVRWPSGCLIIGLRRGESEIVPRGDTRLRAGDYLV
HHHHCCCHHHCCHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEECCCEEE
VLFSGEEEREVRTAMRRLCDARLD
EEECCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cl Ion [Periplasm] [C]

Specific reaction: Cl Ion [Periplasm] = Cl Ion [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA