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The map label for this gene is purA [H]

Identifier: 213691044

GI number: 213691044

Start: 144041

End: 145327

Strand: Direct

Name: purA [H]

Synonym: Blon_0123

Alternate gene names: 213691044

Gene position: 144041-145327 (Clockwise)

Preceding gene: 213691043

Following gene: 213691045

Centisome position: 5.08

GC content: 63.17

Gene sequence:

>1287_bases
ATGCCTGGAATTGTTCTTATTGGTGCTCAGTGGGGCGACGAGGGTAAAGGCAAGGCTACCGATCTGATCGGCACCAAGGT
CGATTACGTCGCGCGCTTCAACGGCGGCAACAACGCGGGCCATAGCGTGGTGGTCGGCGATGAGTCGTACGCCCTGCACC
TGCTGCCCTCCGGCATCATCAACCCGAACCTGACCCCGGTGATCGGCAACGGCGTGGTCGTGGACCCGGAAGTGCTCTTC
GAGGAGATTGACGGCCTGGAATCCCGCGGCATCGATTGCTCCCACCTCAAGGTGAGCGAGGCCGCGCACATCATCGCGCC
CTACCACCGCACACTCGACAAGGTGACTGAACGCTTCCTTGGCAAGCACAAGATCGGCACCACCGGCCGTGGCATCGGCC
CAGCCTACGCGGACAAGATCAACCGTGTCGGCATCCGCGTGCATGACCTGTTCAACGCCGACCATCTGCACGACAAGGTG
GAGGCCAGCCTGCACCAGAAGAACCAGATGCTGGTCAAGCTCTACAACCGTCGTCCGATCGACGTGGACCAGACCACCGA
GGAGCTGCTGAAGCTCGGCGAACGCCTCAAGCCGTACGTGGCCAACACCGGCCTGATTCTCAACAAGGCGCTTGACGAAG
GCAAGACCGTACTGTTTGAAGGCGCTCAGGCCACCATGCTTGACGTGGACCACGGCACCTACCCGTTCGTCACCTCTTCC
AACCCGACCGCCGGCGGCGCCTGCACCGGCACCGGCGTGGGCCCCACCAAGATCACCCGCGTAATCGGCGTCTCCAAGGC
CTACGTCACCCGCGTGGGCGAAGGCCCATTCCCCACCGAACTGCTGGACGAGTCCGGCGAATGGCTGCGTCAGCAGGGCC
ACGAATTCGGCGTGACCACCGGCCGCCCGCGTCGCTGTGGTTGGTTCGATGCAGTCGTCAACCGGTACGCCTCCCAGGTC
AACGGCCTGACCGACATCGTGCTCACCAAGCTCGACGTGCTCACCGGCCTGAAGGAGATTCCGATCTGCGTGGCTTACGA
CGTGGACGGCGAACGTCACGACGATATGCCGACCGATCAGGCCGCTTTCGCCGCCGCCAAGCCGATTTACGAGACCATGC
CCGGCTGGGATGAAGACATCTCCGACTGCCACTCCTTCGACGAGCTGCCCGCCACCTGCCAGGCCTACGTCAAGCGTTTG
GAAGAGCTGAGCGGCTGCCGTATCTCCGTGATCGGCACCGGCCCACAGCGCGACCATGTCATCCAGATCAATTCGCTGGT
CGACTGA

Upstream 100 bases:

>100_bases
GGTAGCTTGGACAGGCCGGCGGCTGCATATGGCGTGGCAGCGGTAGAGGCTGTACGGTAGTCTGTGTTCGGCGGAAAAGG
CGATGTTGCGGAGGTGTGTT

Downstream 100 bases:

>100_bases
CCGTCTGCAACGAATTTCTCGGCTTGACTGGTAACGACGCGGCACATGATATCCGCCGGCTGATTCCGCGATTCGACCAC
TTGAAGTGGAAGATGGCGGC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MPGIVLIGAQWGDEGKGKATDLIGTKVDYVARFNGGNNAGHSVVVGDESYALHLLPSGIINPNLTPVIGNGVVVDPEVLF
EEIDGLESRGIDCSHLKVSEAAHIIAPYHRTLDKVTERFLGKHKIGTTGRGIGPAYADKINRVGIRVHDLFNADHLHDKV
EASLHQKNQMLVKLYNRRPIDVDQTTEELLKLGERLKPYVANTGLILNKALDEGKTVLFEGAQATMLDVDHGTYPFVTSS
NPTAGGACTGTGVGPTKITRVIGVSKAYVTRVGEGPFPTELLDESGEWLRQQGHEFGVTTGRPRRCGWFDAVVNRYASQV
NGLTDIVLTKLDVLTGLKEIPICVAYDVDGERHDDMPTDQAAFAAAKPIYETMPGWDEDISDCHSFDELPATCQAYVKRL
EELSGCRISVIGTGPQRDHVIQINSLVD

Sequences:

>Translated_428_residues
MPGIVLIGAQWGDEGKGKATDLIGTKVDYVARFNGGNNAGHSVVVGDESYALHLLPSGIINPNLTPVIGNGVVVDPEVLF
EEIDGLESRGIDCSHLKVSEAAHIIAPYHRTLDKVTERFLGKHKIGTTGRGIGPAYADKINRVGIRVHDLFNADHLHDKV
EASLHQKNQMLVKLYNRRPIDVDQTTEELLKLGERLKPYVANTGLILNKALDEGKTVLFEGAQATMLDVDHGTYPFVTSS
NPTAGGACTGTGVGPTKITRVIGVSKAYVTRVGEGPFPTELLDESGEWLRQQGHEFGVTTGRPRRCGWFDAVVNRYASQV
NGLTDIVLTKLDVLTGLKEIPICVAYDVDGERHDDMPTDQAAFAAAKPIYETMPGWDEDISDCHSFDELPATCQAYVKRL
EELSGCRISVIGTGPQRDHVIQINSLVD
>Mature_427_residues
PGIVLIGAQWGDEGKGKATDLIGTKVDYVARFNGGNNAGHSVVVGDESYALHLLPSGIINPNLTPVIGNGVVVDPEVLFE
EIDGLESRGIDCSHLKVSEAAHIIAPYHRTLDKVTERFLGKHKIGTTGRGIGPAYADKINRVGIRVHDLFNADHLHDKVE
ASLHQKNQMLVKLYNRRPIDVDQTTEELLKLGERLKPYVANTGLILNKALDEGKTVLFEGAQATMLDVDHGTYPFVTSSN
PTAGGACTGTGVGPTKITRVIGVSKAYVTRVGEGPFPTELLDESGEWLRQQGHEFGVTTGRPRRCGWFDAVVNRYASQVN
GLTDIVLTKLDVLTGLKEIPICVAYDVDGERHDDMPTDQAAFAAAKPIYETMPGWDEDISDCHSFDELPATCQAYVKRLE
ELSGCRISVIGTGPQRDHVIQINSLVD

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=428, Percent_Identity=44.6261682242991, Blast_Score=348, Evalue=5e-96,
Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=43.4272300469484, Blast_Score=348, Evalue=7e-96,
Organism=Homo sapiens, GI40316944, Length=396, Percent_Identity=42.4242424242424, Blast_Score=310, Evalue=2e-84,
Organism=Escherichia coli, GI1790620, Length=427, Percent_Identity=47.7751756440281, Blast_Score=407, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25146553, Length=429, Percent_Identity=44.2890442890443, Blast_Score=329, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI25146555, Length=429, Percent_Identity=44.2890442890443, Blast_Score=329, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6324109, Length=430, Percent_Identity=44.4186046511628, Blast_Score=372, Evalue=1e-104,
Organism=Drosophila melanogaster, GI21356033, Length=423, Percent_Identity=43.4988179669031, Blast_Score=343, Evalue=1e-94,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46497; Mature: 46366

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGIVLIGAQWGDEGKGKATDLIGTKVDYVARFNGGNNAGHSVVVGDESYALHLLPSGII
CCCEEEEECCCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCEEEECCCCEEEEECCCCCC
NPNLTPVIGNGVVVDPEVLFEEIDGLESRGIDCSHLKVSEAAHIIAPYHRTLDKVTERFL
CCCCCEEECCCEEECHHHHHHHHCCHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
GKHKIGTTGRGIGPAYADKINRVGIRVHDLFNADHLHDKVEASLHQKNQMLVKLYNRRPI
CCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
DVDQTTEELLKLGERLKPYVANTGLILNKALDEGKTVLFEGAQATMLDVDHGTYPFVTSS
CCCHHHHHHHHHHHHHCCHHHCCCEEEEHHHCCCCEEEEECCCEEEEECCCCCCCEEECC
NPTAGGACTGTGVGPTKITRVIGVSKAYVTRVGEGPFPTELLDESGEWLRQQGHEFGVTT
CCCCCCCCCCCCCCHHHHHHHHHCCHHHHHCCCCCCCCHHHHHCCHHHHHHCCCEECCCC
GRPRRCGWFDAVVNRYASQVNGLTDIVLTKLDVLTGLKEIPICVAYDVDGERHDDMPTDQ
CCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHCCEEEEEECCCCCCCCCCCCH
AAFAAAKPIYETMPGWDEDISDCHSFDELPATCQAYVKRLEELSGCRISVIGTGPQRDHV
HHHHHHCCHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCE
IQINSLVD
EEECCCCC
>Mature Secondary Structure 
PGIVLIGAQWGDEGKGKATDLIGTKVDYVARFNGGNNAGHSVVVGDESYALHLLPSGII
CCEEEEECCCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCEEEECCCCEEEEECCCCCC
NPNLTPVIGNGVVVDPEVLFEEIDGLESRGIDCSHLKVSEAAHIIAPYHRTLDKVTERFL
CCCCCEEECCCEEECHHHHHHHHCCHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
GKHKIGTTGRGIGPAYADKINRVGIRVHDLFNADHLHDKVEASLHQKNQMLVKLYNRRPI
CCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
DVDQTTEELLKLGERLKPYVANTGLILNKALDEGKTVLFEGAQATMLDVDHGTYPFVTSS
CCCHHHHHHHHHHHHHCCHHHCCCEEEEHHHCCCCEEEEECCCEEEEECCCCCCCEEECC
NPTAGGACTGTGVGPTKITRVIGVSKAYVTRVGEGPFPTELLDESGEWLRQQGHEFGVTT
CCCCCCCCCCCCCCHHHHHHHHHCCHHHHHCCCCCCCCHHHHHCCHHHHHHCCCEECCCC
GRPRRCGWFDAVVNRYASQVNGLTDIVLTKLDVLTGLKEIPICVAYDVDGERHDDMPTDQ
CCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHCCEEEEEECCCCCCCCCCCCH
AAFAAAKPIYETMPGWDEDISDCHSFDELPATCQAYVKRLEELSGCRISVIGTGPQRDHV
HHHHHHCCHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCE
IQINSLVD
EEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA