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The map label for this gene is opuE [H]

Identifier: 213690983

GI number: 213690983

Start: 71599

End: 73260

Strand: Direct

Name: opuE [H]

Synonym: Blon_0061

Alternate gene names: 213690983

Gene position: 71599-73260 (Clockwise)

Preceding gene: 213690982

Following gene: 213690985

Centisome position: 2.53

GC content: 63.18

Gene sequence:

>1662_bases
ATGATTGAGGAGGGGTCGCAGTTGGCCAACGATTTCTGGATTCTGCTGGCCATGGTCATCTATTTTGCGGCCATGCTGTC
CATCGGATTCATCTACTCCAAACGCTCCAACTCCTCCTCCAAGGAATACTTCGCGGGCGGGCGCGGCGTGGGGCCGTGGC
TCACGGCCCTGAGCGCCGAAGCCTCCGACATGAGCGGCTGGCTGCTGATGGGCCTGCCGGGCCTGGCCTACTTCACCGGC
GCGGCCGACCCGATGTGGACCGCCATCGGCCTGGCGCTCGGCACCTATCTCAACTGGAAGCTTGTGGCCCGGCGACTGCG
CCGCTACTCGGTAGTGGCCGGCGATGCCATCACCATTCCTGACTTCTTCGCCAAGCGTTTCCATGACAAGAAGGGCGTGG
TCTCCACCATCGCGGCCGTGATCATCCTGGTGTTCTTCAGCGTGTACGTGGGCAGCTGCTTCGTGACCGTGGGCAAGCTG
TTCGCCACGTTGTTCGGTTTCGACTACCACGTCATGATGATCATCGGCGCGGTGGTCGTGTTCGTCTACACCGTGGTGGG
CGGCTACTTGTCCGTGGTGATGACCGACTTCATTCAAGGCATGCTCATGTTCTTCGCGCTCGCGGTGGTGTTCATCGGCA
CCGTGGCCAGCGCGGGCGGCATCGACAACACCGTGGAATTCCTGCGTGCCATCCCCGGCTACCTGTCCGGCACGCAGGTG
GCCGCGCCCAAGCTGGACCCGGCCACCGGCCAGCAGCTCGTCGAGGCGGGCAAGGCCGTGTTCGGCGCGCCCTCCGACTA
CGGCATCATCACCATCATCTCCATGCTGGCGTGGGGCCTGGGTTACTTCGGTATGCCGCAGGTGCTGGTGCGCTTCCTGT
CCATCCGCAGCGTGGAAGAAGTGCGCAAGTCCCGAATCATCGCCACCTCCTGGTGCGTGATTTCGCTGGGTTGCGCGGTG
TGCATCGGCTTGGTGGGGCGCGCGATGATGCCCACCGAACTGCTGACCTCCACCGATGCGGAAACCATTTTCATCGTGCT
GGCGCAGACCCTGCTGCCGAGCTTCATGTGCGGCGTGGTGGTCTCCGGCATTTTCGCGGCCTCGATGAGCTCCAGCTCCT
CGTATCTGATCATCGGCGCTTCGGCCATGGGCGAGAACATCTTCCGCGGGCTGCTGCACCGCAAGGCCACCGACAAGCAG
GTCATGATCGTGGCCAGGGTCACGCTGGTGGTCATGTTCCTGTTCGGTATTCTGGTGGCATACGACCAGAACTCGTCGAT
CTTCCAGGTGGTGTCTTACGCGTGGGCCGGGCTTGGCGCCTCATTCGGCCCGTTGATGCTGTGCTCGCTATACTGGCGTC
GCGCCAACAAGGCCGGCGCCGTGGCCGGCATGCTGTCCGGCACGGCGGCCGTGCTGATCTGGCACAACCTCGTCAAGCCC
TTGGGCGGCGTATTCGCCATCTATGAGCTGCTGCCGGCGTTCATCATTTCGCTGCTGTTCATCGTGGTGGTTTCGTTGCT
GACCGCCAAGCCGAACGAGCGTATGCTCTACGAGTTCGACCACTACATGGACGATCCGCTGCCCGGCACCCTGCCGTCCG
GCACCGTGCTCGTCGACGAGCCCATGGAAGCCATGGAGTCCGAGGTCCGCAGCGGCAAATGA

Upstream 100 bases:

>100_bases
TTGCGGCCGTATTCACGGCTGTGACGGTCTGTATGACGCCGCTCCGGCGTCAATCGCATATCAGCACATAGTTCTGCCAT
GGCGGTAGCCGCTTGGCGTC

Downstream 100 bases:

>100_bases
CACGTGCCTGCTGTGCGGTCGGCTCGTATGGCAGAGGAGTGGCTTTTTTGTGGTTCGGCAGGAACCATTGAGGCCAAAAC
GGACTTCGAGGGGCTGATTT

Product: SSS sodium solute transporter superfamily

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MIEEGSQLANDFWILLAMVIYFAAMLSIGFIYSKRSNSSSKEYFAGGRGVGPWLTALSAEASDMSGWLLMGLPGLAYFTG
AADPMWTAIGLALGTYLNWKLVARRLRRYSVVAGDAITIPDFFAKRFHDKKGVVSTIAAVIILVFFSVYVGSCFVTVGKL
FATLFGFDYHVMMIIGAVVVFVYTVVGGYLSVVMTDFIQGMLMFFALAVVFIGTVASAGGIDNTVEFLRAIPGYLSGTQV
AAPKLDPATGQQLVEAGKAVFGAPSDYGIITIISMLAWGLGYFGMPQVLVRFLSIRSVEEVRKSRIIATSWCVISLGCAV
CIGLVGRAMMPTELLTSTDAETIFIVLAQTLLPSFMCGVVVSGIFAASMSSSSSYLIIGASAMGENIFRGLLHRKATDKQ
VMIVARVTLVVMFLFGILVAYDQNSSIFQVVSYAWAGLGASFGPLMLCSLYWRRANKAGAVAGMLSGTAAVLIWHNLVKP
LGGVFAIYELLPAFIISLLFIVVVSLLTAKPNERMLYEFDHYMDDPLPGTLPSGTVLVDEPMEAMESEVRSGK

Sequences:

>Translated_553_residues
MIEEGSQLANDFWILLAMVIYFAAMLSIGFIYSKRSNSSSKEYFAGGRGVGPWLTALSAEASDMSGWLLMGLPGLAYFTG
AADPMWTAIGLALGTYLNWKLVARRLRRYSVVAGDAITIPDFFAKRFHDKKGVVSTIAAVIILVFFSVYVGSCFVTVGKL
FATLFGFDYHVMMIIGAVVVFVYTVVGGYLSVVMTDFIQGMLMFFALAVVFIGTVASAGGIDNTVEFLRAIPGYLSGTQV
AAPKLDPATGQQLVEAGKAVFGAPSDYGIITIISMLAWGLGYFGMPQVLVRFLSIRSVEEVRKSRIIATSWCVISLGCAV
CIGLVGRAMMPTELLTSTDAETIFIVLAQTLLPSFMCGVVVSGIFAASMSSSSSYLIIGASAMGENIFRGLLHRKATDKQ
VMIVARVTLVVMFLFGILVAYDQNSSIFQVVSYAWAGLGASFGPLMLCSLYWRRANKAGAVAGMLSGTAAVLIWHNLVKP
LGGVFAIYELLPAFIISLLFIVVVSLLTAKPNERMLYEFDHYMDDPLPGTLPSGTVLVDEPMEAMESEVRSGK
>Mature_553_residues
MIEEGSQLANDFWILLAMVIYFAAMLSIGFIYSKRSNSSSKEYFAGGRGVGPWLTALSAEASDMSGWLLMGLPGLAYFTG
AADPMWTAIGLALGTYLNWKLVARRLRRYSVVAGDAITIPDFFAKRFHDKKGVVSTIAAVIILVFFSVYVGSCFVTVGKL
FATLFGFDYHVMMIIGAVVVFVYTVVGGYLSVVMTDFIQGMLMFFALAVVFIGTVASAGGIDNTVEFLRAIPGYLSGTQV
AAPKLDPATGQQLVEAGKAVFGAPSDYGIITIISMLAWGLGYFGMPQVLVRFLSIRSVEEVRKSRIIATSWCVISLGCAV
CIGLVGRAMMPTELLTSTDAETIFIVLAQTLLPSFMCGVVVSGIFAASMSSSSSYLIIGASAMGENIFRGLLHRKATDKQ
VMIVARVTLVVMFLFGILVAYDQNSSIFQVVSYAWAGLGASFGPLMLCSLYWRRANKAGAVAGMLSGTAAVLIWHNLVKP
LGGVFAIYELLPAFIISLLFIVVVSLLTAKPNERMLYEFDHYMDDPLPGTLPSGTVLVDEPMEAMESEVRSGK

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=513, Percent_Identity=40.7407407407407, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI109659836, Length=551, Percent_Identity=24.6823956442831, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI14140236, Length=544, Percent_Identity=21.3235294117647, Blast_Score=87, Evalue=6e-17,
Organism=Homo sapiens, GI109659839, Length=567, Percent_Identity=23.9858906525573, Blast_Score=87, Evalue=6e-17,
Organism=Homo sapiens, GI4507031, Length=546, Percent_Identity=22.8937728937729, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI110835708, Length=575, Percent_Identity=20.8695652173913, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI206597483, Length=387, Percent_Identity=22.9974160206718, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI206597487, Length=387, Percent_Identity=22.9974160206718, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI17941285, Length=481, Percent_Identity=22.6611226611227, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1787251, Length=521, Percent_Identity=37.236084452975, Blast_Score=331, Evalue=8e-92,
Organism=Escherichia coli, GI87082237, Length=462, Percent_Identity=25.7575757575758, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790503, Length=514, Percent_Identity=24.3190661478599, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 59595; Mature: 59595

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]