Click here to switch to the map view.

The map label for this gene is phoB [H]

Identifier: 213159117

GI number: 213159117

Start: 3949101

End: 3949811

Strand: Direct

Name: phoB [H]

Synonym: AB57_3827

Alternate gene names: 213159117

Gene position: 3949101-3949811 (Clockwise)

Preceding gene: 213159116

Following gene: 213159118

Centisome position: 97.5

GC content: 44.87

Gene sequence:

>711_bases
GTGAAAGATGACTATATTTTAATTGTCGATGATGAGCTTCCTATCCGCGAAATGATCCATACCTCTTTGGACATGGCAGG
CTTTCAGTGTTTACAGGCAGAAGATGCCAAACAGGCTCATCAAATTATCGTCGACCAACGTCCGGCGCTTATCTTGCTCG
ATTGGATGTTACCCGGGGGCGTAAGTGGGGTCGACTTATGTCGCCGTTTAAAACGTGATGAAAACTTAGCAGAAATTCCA
GTCATTATGCTCACCGCTCGCGGTGAAGAAGACCACAAAGTACAAGGTCTGGATGCTGGTGCAGATGACTATATGACCAA
ACCATTTTCAACCCGTGAACTGGTTTCTCGTATTAAAGCTGTTCTACGCCGAGCAAATGCATTAAGCGGTGAAAAAACAA
TTGATGCCAATGGTTTAATTCTTGATCCTGTAAGCCAACGCGTAAGCTTTGGTAACAGTATTTTAGATATGGGACCAACA
GAATACCGTCTGCTTGCATTTTTTATGACCCATCCGGAACGTGCCTATACACGTGCCCAGTTGCTCGATCAGGTGTGGGG
CGGTAATGTATACATCGAAGATCGTACTATCGATGTGCATATCCGTCGTTTGCGTAAAGTCTTAGAACCTTTTGGTGTAG
ACCGATTTGTTCAGACTGTACGTGGTACAGGTTATCGTTTCTCAACACGTGCAGATTTAGCCGCAGGTTAA

Upstream 100 bases:

>100_bases
TATAGACTTTTCTGCAATGAAATTGTCATAATTAATCTTTGTAATAAGCCTGTCATAACTACAAAACGGTTCACCGTTAA
AATCTACAAGGTATTACGCT

Downstream 100 bases:

>100_bases
CCAAAATTTATGTATGAACCCTACCCCGTCCCCGAACAGGTACGTGAACAAAAGCTTTCCCGCTACAGTAGTTTATGGAC
GTTTGCTAAACAAGATTTAC

Product: phosphate regulon transcriptional regulatory protein PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MKDDYILIVDDELPIREMIHTSLDMAGFQCLQAEDAKQAHQIIVDQRPALILLDWMLPGGVSGVDLCRRLKRDENLAEIP
VIMLTARGEEDHKVQGLDAGADDYMTKPFSTRELVSRIKAVLRRANALSGEKTIDANGLILDPVSQRVSFGNSILDMGPT
EYRLLAFFMTHPERAYTRAQLLDQVWGGNVYIEDRTIDVHIRRLRKVLEPFGVDRFVQTVRGTGYRFSTRADLAAG

Sequences:

>Translated_236_residues
MKDDYILIVDDELPIREMIHTSLDMAGFQCLQAEDAKQAHQIIVDQRPALILLDWMLPGGVSGVDLCRRLKRDENLAEIP
VIMLTARGEEDHKVQGLDAGADDYMTKPFSTRELVSRIKAVLRRANALSGEKTIDANGLILDPVSQRVSFGNSILDMGPT
EYRLLAFFMTHPERAYTRAQLLDQVWGGNVYIEDRTIDVHIRRLRKVLEPFGVDRFVQTVRGTGYRFSTRADLAAG
>Mature_236_residues
MKDDYILIVDDELPIREMIHTSLDMAGFQCLQAEDAKQAHQIIVDQRPALILLDWMLPGGVSGVDLCRRLKRDENLAEIP
VIMLTARGEEDHKVQGLDAGADDYMTKPFSTRELVSRIKAVLRRANALSGEKTIDANGLILDPVSQRVSFGNSILDMGPT
EYRLLAFFMTHPERAYTRAQLLDQVWGGNVYIEDRTIDVHIRRLRKVLEPFGVDRFVQTVRGTGYRFSTRADLAAG

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=225, Percent_Identity=62.2222222222222, Blast_Score=288, Evalue=3e-79,
Organism=Escherichia coli, GI1788394, Length=228, Percent_Identity=37.280701754386, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1789809, Length=235, Percent_Identity=37.4468085106383, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=35, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1786911, Length=225, Percent_Identity=35.1111111111111, Blast_Score=130, Evalue=6e-32,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=36.322869955157, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI2367329, Length=234, Percent_Identity=38.034188034188, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1790863, Length=237, Percent_Identity=34.5991561181435, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1790860, Length=227, Percent_Identity=33.920704845815, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=31.1111111111111, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=29.7777777777778, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=33.1818181818182, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787229, Length=228, Percent_Identity=30.7017543859649, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI145693140, Length=240, Percent_Identity=27.9166666666667, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1788550, Length=111, Percent_Identity=34.2342342342342, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790299, Length=146, Percent_Identity=26.027397260274, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1788191, Length=124, Percent_Identity=25.8064516129032, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26642; Mature: 26642

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDDYILIVDDELPIREMIHTSLDMAGFQCLQAEDAKQAHQIIVDQRPALILLDWMLPGG
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
VSGVDLCRRLKRDENLAEIPVIMLTARGEEDHKVQGLDAGADDYMTKPFSTRELVSRIKA
CCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEECCCCCCHHHCCCCCCHHHHHHHHHH
VLRRANALSGEKTIDANGLILDPVSQRVSFGNSILDMGPTEYRLLAFFMTHPERAYTRAQ
HHHHHHCCCCCCEECCCCEEECCHHHHHHCCCCHHCCCCCHHHHHHHHHHCCHHHHHHHH
LLDQVWGGNVYIEDRTIDVHIRRLRKVLEPFGVDRFVQTVRGTGYRFSTRADLAAG
HHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCCCC
>Mature Secondary Structure
MKDDYILIVDDELPIREMIHTSLDMAGFQCLQAEDAKQAHQIIVDQRPALILLDWMLPGG
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
VSGVDLCRRLKRDENLAEIPVIMLTARGEEDHKVQGLDAGADDYMTKPFSTRELVSRIKA
CCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEECCCCCCHHHCCCCCCHHHHHHHHHH
VLRRANALSGEKTIDANGLILDPVSQRVSFGNSILDMGPTEYRLLAFFMTHPERAYTRAQ
HHHHHHCCCCCCEECCCCEEECCHHHHHHCCCCHHCCCCCHHHHHHHHHHCCHHHHHHHH
LLDQVWGGNVYIEDRTIDVHIRRLRKVLEPFGVDRFVQTVRGTGYRFSTRADLAAG
HHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2115874; 10984043 [H]