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The map label for this gene is aceF [C]

Identifier: 213159066

GI number: 213159066

Start: 3893139

End: 3895118

Strand: Reverse

Name: aceF [C]

Synonym: AB57_3776

Alternate gene names: 213159066

Gene position: 3895118-3893139 (Counterclockwise)

Preceding gene: 213159067

Following gene: 213159065

Centisome position: 96.16

GC content: 44.7

Gene sequence:

>1980_bases
ATGCAAATTAAGACCCCTGATATTGGTGTAGATAAAGCAAACGTCGCTGAAATTTTAGTAAAAGTTGGCGACCGTGTTGA
AGTTGACGACAGTATCGTTGTGCTTGAGTCTGATAAAGCAACTGTTGAAGTGCCTAGCACTTCAGCAGGTGTCGTAAAAA
GTATCTTGATTAATCAAGGTGATGATGTGACTGAAGGTGTTGCTTTGATTGAGATCGAAGCAGAAGGTGCGGCACAAGCA
GCACCGGAACCAACACCAGCTCCTGCGGCTGAAAAGCCAGCTGCTCCAGCACCTGCGCAACAAACTCAAGCTTCAGCTCA
ACCTGCTGCTACATCAAGTGCAACTGTTGAAGTGACTGTACCTGACATCGGTGTTGAAAAAGCATTGGTTGGTGAAATCC
TCGTTAAAGTGGGTGACCAGATCGATGTTGAACAAAGTATTGTGGTTGTAGAGTCAGATAAAGCGACTGTAGAAGTTCCA
AGTAGTGTTGCTGGTACTGTAGAAAGTATTCAAGTGAAAGAAGGCGATACTGTTAAAGAAGGTGTTGTTCTTATTCAAGT
GAAAACAGCAGCTGCATCTAATGCGCAAGCAGAAGCTCCTGCTACTACACCAGCTCCAGCTGCGGTAGCAGAACCGGTTG
CTGCAAAGCAAGAAACTGTGGCAGCGGCTCCAGCTCAGTCTGGTTCAGTTGATATCAATGTTCCTGATTTGGGTGTAGAC
AAAGCTATTGTTGCTGAAATCTTGGTTCAAGTTGGCGATAAAGTTGATGTAGACCAAAGCCTTGTTGTTGTTGAGTCAGA
TAAAGCAACTGTGGAAGTCCCAAGTACCGTTGCTGGTGTTGTAAAAGCAATTCACTTGCAAGCAGGCCAACAAGTTTCAC
AAGGTGTATTGCTTGCAACAATTGAAGCTGAAGGCCAAGCACCTGCTGCTGCACCAGCGGCAAAAGCAGAAGCAGCCCCA
GCTCCACAGGCAGCAGCACCTAAAGCAGCCGCTCCTGTTGCAACTCAGTCTGCACCTGCGGCACCTGCATCTGGTACTGA
TAAGTTAACGAAAGAGCAAGAAGCAGAAAACGCTAAAGTGTATGCTGGTCCTGCTGTTCGTAAGCTGGCTCGTGAACTTG
GTGTGATCTTGTCACAAGTTAAAACTTCTGGTGAGCATGGCCGTGTTGTTAAAGAAGATATCTTTGCTTATGTGAAGAGC
CGTTTAACTGCACCGCAAGCTGCACCAGTAGCTCAAGCTACTGCTGCTCCGGCTGGATTGCCGTCATTACCTGACTTTAC
TGCTTTTGGTGGCGGTGAAGTAAAACCAATGACGCGTTTACAGCAAGTTTCTGTACCGCAGTTGTCTTTAAACAACTATA
TTCCACAAGTAACTCAGTTTGACCTTGCGGATATTACTGAGCTAGAAGCTTGGCGTGGTGAGCTGAAAGACGGCTTTAAG
AAACAAGGTGTGAGCCTAACAATTTTGGCATTTATTGCAAAAGCAGTAGCACACTTGTTGAAAGAAGAGCCTTATTTTGC
GGGTCACTTAGCAGATGATCAGAAATCTGTATTGCTCCGTAATGAAATCCATATGGGTATTGCGGTTGCAACTCCAGATG
GTTTGACCGTGCCTGTACTACGTAATCCTGACCAAAAATCAATTAAGCAAATTGCTGTTGAATTAGGTGAGTTGAGTAAA
AAAGCGCGTGATAAGAAATTAACGCCGAAAGATTTACAAGGTGCTAACTTCACGATCACAAGCTTAGGCTCAATTGGCGG
TACAGCATTTACGCCACTAGTTAACTGGCCACAAGTTGCAATTTTGGGTATTTCACCTGCAACGATGCAGCCTGTATGGA
ATGGTAAAGACTTTGATCCACGCTTAATGTTGCCGTTGTCATTGTCTTATGACCACCGTGTAATTAATGGTGCGGATGCA
GCGCGCTTTACAAATAAACTTACGAAACTTCTTAAAGATATTCGTACTTTATTAATCTAA

Upstream 100 bases:

>100_bases
TAGATACTGACCGTCCGGTTGCATGGGCGCCGCAAGCACATCCGGAAGTTCAGCCAGTTGCCGAATACAATGAAACGCAA
ACAGGTGAGGGGAACTAAGC

Downstream 100 bases:

>100_bases
TGCTTTGAAAGAACATACTAAAACCTCGCTTCGGCGGGGTTTTGTTTTAATGTCTATTTGACTTAATTTTAGGTTAATCT
ACAGATTAAGCAGGACTTAT

Product: pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 659; Mature: 659

Protein sequence:

>659_residues
MQIKTPDIGVDKANVAEILVKVGDRVEVDDSIVVLESDKATVEVPSTSAGVVKSILINQGDDVTEGVALIEIEAEGAAQA
APEPTPAPAAEKPAAPAPAQQTQASAQPAATSSATVEVTVPDIGVEKALVGEILVKVGDQIDVEQSIVVVESDKATVEVP
SSVAGTVESIQVKEGDTVKEGVVLIQVKTAAASNAQAEAPATTPAPAAVAEPVAAKQETVAAAPAQSGSVDINVPDLGVD
KAIVAEILVQVGDKVDVDQSLVVVESDKATVEVPSTVAGVVKAIHLQAGQQVSQGVLLATIEAEGQAPAAAPAAKAEAAP
APQAAAPKAAAPVATQSAPAAPASGTDKLTKEQEAENAKVYAGPAVRKLARELGVILSQVKTSGEHGRVVKEDIFAYVKS
RLTAPQAAPVAQATAAPAGLPSLPDFTAFGGGEVKPMTRLQQVSVPQLSLNNYIPQVTQFDLADITELEAWRGELKDGFK
KQGVSLTILAFIAKAVAHLLKEEPYFAGHLADDQKSVLLRNEIHMGIAVATPDGLTVPVLRNPDQKSIKQIAVELGELSK
KARDKKLTPKDLQGANFTITSLGSIGGTAFTPLVNWPQVAILGISPATMQPVWNGKDFDPRLMLPLSLSYDHRVINGADA
ARFTNKLTKLLKDIRTLLI

Sequences:

>Translated_659_residues
MQIKTPDIGVDKANVAEILVKVGDRVEVDDSIVVLESDKATVEVPSTSAGVVKSILINQGDDVTEGVALIEIEAEGAAQA
APEPTPAPAAEKPAAPAPAQQTQASAQPAATSSATVEVTVPDIGVEKALVGEILVKVGDQIDVEQSIVVVESDKATVEVP
SSVAGTVESIQVKEGDTVKEGVVLIQVKTAAASNAQAEAPATTPAPAAVAEPVAAKQETVAAAPAQSGSVDINVPDLGVD
KAIVAEILVQVGDKVDVDQSLVVVESDKATVEVPSTVAGVVKAIHLQAGQQVSQGVLLATIEAEGQAPAAAPAAKAEAAP
APQAAAPKAAAPVATQSAPAAPASGTDKLTKEQEAENAKVYAGPAVRKLARELGVILSQVKTSGEHGRVVKEDIFAYVKS
RLTAPQAAPVAQATAAPAGLPSLPDFTAFGGGEVKPMTRLQQVSVPQLSLNNYIPQVTQFDLADITELEAWRGELKDGFK
KQGVSLTILAFIAKAVAHLLKEEPYFAGHLADDQKSVLLRNEIHMGIAVATPDGLTVPVLRNPDQKSIKQIAVELGELSK
KARDKKLTPKDLQGANFTITSLGSIGGTAFTPLVNWPQVAILGISPATMQPVWNGKDFDPRLMLPLSLSYDHRVINGADA
ARFTNKLTKLLKDIRTLLI
>Mature_659_residues
MQIKTPDIGVDKANVAEILVKVGDRVEVDDSIVVLESDKATVEVPSTSAGVVKSILINQGDDVTEGVALIEIEAEGAAQA
APEPTPAPAAEKPAAPAPAQQTQASAQPAATSSATVEVTVPDIGVEKALVGEILVKVGDQIDVEQSIVVVESDKATVEVP
SSVAGTVESIQVKEGDTVKEGVVLIQVKTAAASNAQAEAPATTPAPAAVAEPVAAKQETVAAAPAQSGSVDINVPDLGVD
KAIVAEILVQVGDKVDVDQSLVVVESDKATVEVPSTVAGVVKAIHLQAGQQVSQGVLLATIEAEGQAPAAAPAAKAEAAP
APQAAAPKAAAPVATQSAPAAPASGTDKLTKEQEAENAKVYAGPAVRKLARELGVILSQVKTSGEHGRVVKEDIFAYVKS
RLTAPQAAPVAQATAAPAGLPSLPDFTAFGGGEVKPMTRLQQVSVPQLSLNNYIPQVTQFDLADITELEAWRGELKDGFK
KQGVSLTILAFIAKAVAHLLKEEPYFAGHLADDQKSVLLRNEIHMGIAVATPDGLTVPVLRNPDQKSIKQIAVELGELSK
KARDKKLTPKDLQGANFTITSLGSIGGTAFTPLVNWPQVAILGISPATMQPVWNGKDFDPRLMLPLSLSYDHRVINGADA
ARFTNKLTKLLKDIRTLLI

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=434, Percent_Identity=30.4147465437788, Blast_Score=159, Evalue=1e-38,
Organism=Homo sapiens, GI19923748, Length=225, Percent_Identity=35.1111111111111, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI31711992, Length=439, Percent_Identity=29.3849658314351, Blast_Score=126, Evalue=6e-29,
Organism=Homo sapiens, GI203098816, Length=473, Percent_Identity=25.369978858351, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI203098753, Length=459, Percent_Identity=25.2723311546841, Blast_Score=93, Evalue=9e-19,
Organism=Homo sapiens, GI260898739, Length=129, Percent_Identity=37.2093023255814, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1786305, Length=665, Percent_Identity=44.3609022556391, Blast_Score=443, Evalue=1e-125,
Organism=Escherichia coli, GI1786946, Length=435, Percent_Identity=32.183908045977, Blast_Score=167, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=441, Percent_Identity=28.1179138321995, Blast_Score=163, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI25146366, Length=210, Percent_Identity=36.6666666666667, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17560088, Length=433, Percent_Identity=28.175519630485, Blast_Score=119, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17538894, Length=307, Percent_Identity=27.0358306188925, Blast_Score=89, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6320352, Length=459, Percent_Identity=29.1938997821351, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324258, Length=370, Percent_Identity=27.2972972972973, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI18859875, Length=448, Percent_Identity=26.7857142857143, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24645909, Length=197, Percent_Identity=33.502538071066, Blast_Score=120, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24582497, Length=419, Percent_Identity=27.9236276849642, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI20129315, Length=195, Percent_Identity=32.3076923076923, Blast_Score=100, Evalue=5e-21,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 68189; Mature: 68189

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIKTPDIGVDKANVAEILVKVGDRVEVDDSIVVLESDKATVEVPSTSAGVVKSILINQG
CCCCCCCCCCCHHHHHHHHHHCCCCEEECCCEEEEECCCCEEECCCCCHHHHHHHHHCCC
DDVTEGVALIEIEAEGAAQAAPEPTPAPAAEKPAAPAPAQQTQASAQPAATSSATVEVTV
CCHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEEE
PDIGVEKALVGEILVKVGDQIDVEQSIVVVESDKATVEVPSSVAGTVESIQVKEGDTVKE
CCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEECCCHHHCCHHEEEECCCCCHHC
GVVLIQVKTAAASNAQAEAPATTPAPAAVAEPVAAKQETVAAAPAQSGSVDINVPDLGVD
CEEEEEEECCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHEECCCCCCCEEEECCCCCCC
KAIVAEILVQVGDKVDVDQSLVVVESDKATVEVPSTVAGVVKAIHLQAGQQVSQGVLLAT
HHHHHHHHHHCCCCCCCCCEEEEEECCCCEEECCHHHHHHHHHHHHHCCCHHHCCEEEEE
IEAEGQAPAAAPAAKAEAAPAPQAAAPKAAAPVATQSAPAAPASGTDKLTKEQEAENAKV
EECCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEE
YAGPAVRKLARELGVILSQVKTSGEHGRVVKEDIFAYVKSRLTAPQAAPVAQATAAPAGL
EECHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCC
PSLPDFTAFGGGEVKPMTRLQQVSVPQLSLNNYIPQVTQFDLADITELEAWRGELKDGFK
CCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHH
KQGVSLTILAFIAKAVAHLLKEEPYFAGHLADDQKSVLLRNEIHMGIAVATPDGLTVPVL
HCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHEEEECEEEEEEECCCCCEEEEE
RNPDQKSIKQIAVELGELSKKARDKKLTPKDLQGANFTITSLGSIGGTAFTPLVNWPQVA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCEECCCCCCCCEE
ILGISPATMQPVWNGKDFDPRLMLPLSLSYDHRVINGADAARFTNKLTKLLKDIRTLLI
EEECCCHHCCCCCCCCCCCCEEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQIKTPDIGVDKANVAEILVKVGDRVEVDDSIVVLESDKATVEVPSTSAGVVKSILINQG
CCCCCCCCCCCHHHHHHHHHHCCCCEEECCCEEEEECCCCEEECCCCCHHHHHHHHHCCC
DDVTEGVALIEIEAEGAAQAAPEPTPAPAAEKPAAPAPAQQTQASAQPAATSSATVEVTV
CCHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEEE
PDIGVEKALVGEILVKVGDQIDVEQSIVVVESDKATVEVPSSVAGTVESIQVKEGDTVKE
CCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEECCCHHHCCHHEEEECCCCCHHC
GVVLIQVKTAAASNAQAEAPATTPAPAAVAEPVAAKQETVAAAPAQSGSVDINVPDLGVD
CEEEEEEECCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHEECCCCCCCEEEECCCCCCC
KAIVAEILVQVGDKVDVDQSLVVVESDKATVEVPSTVAGVVKAIHLQAGQQVSQGVLLAT
HHHHHHHHHHCCCCCCCCCEEEEEECCCCEEECCHHHHHHHHHHHHHCCCHHHCCEEEEE
IEAEGQAPAAAPAAKAEAAPAPQAAAPKAAAPVATQSAPAAPASGTDKLTKEQEAENAKV
EECCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEE
YAGPAVRKLARELGVILSQVKTSGEHGRVVKEDIFAYVKSRLTAPQAAPVAQATAAPAGL
EECHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCC
PSLPDFTAFGGGEVKPMTRLQQVSVPQLSLNNYIPQVTQFDLADITELEAWRGELKDGFK
CCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHH
KQGVSLTILAFIAKAVAHLLKEEPYFAGHLADDQKSVLLRNEIHMGIAVATPDGLTVPVL
HCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHEEEECEEEEEEECCCCCEEEEE
RNPDQKSIKQIAVELGELSKKARDKKLTPKDLQGANFTITSLGSIGGTAFTPLVNWPQVA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCEECCCCCCCCEE
ILGISPATMQPVWNGKDFDPRLMLPLSLSYDHRVINGADAARFTNKLTKLLKDIRTLLI
EEECCCHHCCCCCCCCCCCCEEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3292237; 3691494; 3191993; 1549782; 8068086; 9119000 [H]