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The map label for this gene is hisF [H]

Identifier: 213158989

GI number: 213158989

Start: 3809445

End: 3810203

Strand: Direct

Name: hisF [H]

Synonym: AB57_3693

Alternate gene names: 213158989

Gene position: 3809445-3810203 (Clockwise)

Preceding gene: 213158985

Following gene: 213158991

Centisome position: 94.05

GC content: 44.66

Gene sequence:

>759_bases
ATGCTTGCTAAACGTATTATTCCTTGCCTAGACGTTGATAACGGACGCGTAGTTAAAGGCGTTCAATTCCTCGATATTCG
TGATGCGGGCGACCCAGTTGAAGTGGCACGTCGCTATAACGAACAAGGTGCAGACGAAATTACTTTCTTAGACATTACCG
CTACACATCATGGACGCGATACCACTTACCGTACTGTTGAACGTATGGCCGAGACTGTATTCGTGCCATTAACTGTCGGT
GGTGGTGTACGTAAGGTTGAAGATATTCGTGCGTTATTAAATGCAGGTGCAGATAAAGTGAGTATTAACTCGGCTGCCGT
ATTTAACCCTGAATTTGTTCAAGAAGCTTCTCAGCATTTTGGCGCGCAATGTATTGTGGTTGCGATCGATGCCAAAAAAA
CTGGCGACAATAAATGGGAAATCTTTACTCACGGTGGCCGTAAACCAACGGGTATTGATGCCATTGAATGGGCTGTAAAA
ATGGCTGACTACGGTGCGGGTGAGTTACTCATTACCAGTATGGATGCCGATGGTACTAAAGCAGGTTACGATATTGCGTT
AATGCGTGCTATTAATGACCGCGTGACCATTCCAACGATCGCATCAGGCGGTGTAGGTAACTTACAACATCTTGCAGACG
GTATTTTACAAGGTGGTGCCGATGCAGTATTGGCAGCAAGTATTTTCCACTTTGGCCAATATACAATTCCAGAAGCTAAA
CAATATTTGGCTGAACAAGGTATCGAAATGCGCTTGTAA

Upstream 100 bases:

>100_bases
AAAAACATCTGTGAAGTTTGATAGAATACCTCTTCCCTCCATGAAGGTGTAGCGCCTGTGTAGGAACTTTCACCAACGGC
GATTATTTTTGGAAAAAGCT

Downstream 100 bases:

>100_bases
GCTTATTTATATTTAAAGGGGCAATATGCCCCTTTAATTTTTTATGCAGCACCTTCTATCGCATTTACATCACTAACGAG
TTTAGGCTGTTCTTTTTTCT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLAKRIIPCLDVDNGRVVKGVQFLDIRDAGDPVEVARRYNEQGADEITFLDITATHHGRDTTYRTVERMAETVFVPLTVG
GGVRKVEDIRALLNAGADKVSINSAAVFNPEFVQEASQHFGAQCIVVAIDAKKTGDNKWEIFTHGGRKPTGIDAIEWAVK
MADYGAGELLITSMDADGTKAGYDIALMRAINDRVTIPTIASGGVGNLQHLADGILQGGADAVLAASIFHFGQYTIPEAK
QYLAEQGIEMRL

Sequences:

>Translated_252_residues
MLAKRIIPCLDVDNGRVVKGVQFLDIRDAGDPVEVARRYNEQGADEITFLDITATHHGRDTTYRTVERMAETVFVPLTVG
GGVRKVEDIRALLNAGADKVSINSAAVFNPEFVQEASQHFGAQCIVVAIDAKKTGDNKWEIFTHGGRKPTGIDAIEWAVK
MADYGAGELLITSMDADGTKAGYDIALMRAINDRVTIPTIASGGVGNLQHLADGILQGGADAVLAASIFHFGQYTIPEAK
QYLAEQGIEMRL
>Mature_252_residues
MLAKRIIPCLDVDNGRVVKGVQFLDIRDAGDPVEVARRYNEQGADEITFLDITATHHGRDTTYRTVERMAETVFVPLTVG
GGVRKVEDIRALLNAGADKVSINSAAVFNPEFVQEASQHFGAQCIVVAIDAKKTGDNKWEIFTHGGRKPTGIDAIEWAVK
MADYGAGELLITSMDADGTKAGYDIALMRAINDRVTIPTIASGGVGNLQHLADGILQGGADAVLAASIFHFGQYTIPEAK
QYLAEQGIEMRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=41.6342412451362, Blast_Score=208, Evalue=2e-55,
Organism=Escherichia coli, GI87082028, Length=241, Percent_Identity=30.7053941908714, Blast_Score=97, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=34.7133757961783, Blast_Score=165, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27186; Mature: 27186

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVDNGRVVKGVQFLDIRDAGDPVEVARRYNEQGADEITFLDITATHHGRD
CCHHHCCCEEECCCCCEEECEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCCC
TTYRTVERMAETVFVPLTVGGGVRKVEDIRALLNAGADKVSINSAAVFNPEFVQEASQHF
HHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCEEEECHHHHHHHHHHC
GAQCIVVAIDAKKTGDNKWEIFTHGGRKPTGIDAIEWAVKMADYGAGELLITSMDADGTK
CCEEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCC
AGYDIALMRAINDRVTIPTIASGGVGNLQHLADGILQGGADAVLAASIFHFGQYTIPEAK
CCCHHEEEEHHCCCEEECEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHH
QYLAEQGIEMRL
HHHHHCCCEECC
>Mature Secondary Structure
MLAKRIIPCLDVDNGRVVKGVQFLDIRDAGDPVEVARRYNEQGADEITFLDITATHHGRD
CCHHHCCCEEECCCCCEEECEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCCC
TTYRTVERMAETVFVPLTVGGGVRKVEDIRALLNAGADKVSINSAAVFNPEFVQEASQHF
HHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCEEEECHHHHHHHHHHC
GAQCIVVAIDAKKTGDNKWEIFTHGGRKPTGIDAIEWAVKMADYGAGELLITSMDADGTK
CCEEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCC
AGYDIALMRAINDRVTIPTIASGGVGNLQHLADGILQGGADAVLAASIFHFGQYTIPEAK
CCCHHEEEEHHCCCEEECEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHH
QYLAEQGIEMRL
HHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA