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The map label for this gene is mdeA [H]

Identifier: 213158971

GI number: 213158971

Start: 3788635

End: 3789912

Strand: Direct

Name: mdeA [H]

Synonym: AB57_3673

Alternate gene names: 213158971

Gene position: 3788635-3789912 (Clockwise)

Preceding gene: 213158969

Following gene: 213158972

Centisome position: 93.53

GC content: 41.78

Gene sequence:

>1278_bases
ATGACTTATAAAGATGAAACCTTAGCCATTCACGCAGGCTACACTCCAGAAGCAACCACTAAAGCTGTTGCAGTCCCTAT
CTATCAAACAACTTCGTATGCATTTGATAACACACAACACGGTGCCGACCTTTTCGATTTAAAAGTTCAAGGCAATATCT
ATACCCGTATTATGAATCCAACCACAGCCGTACTTGAGCAGCGCCTTGCTGCGTTAGAAGGCGGTATTGGGGCATTAGCT
TTGGCATCGGGAATGGCAGCTATTACTTACGCAATTCAGACCATTACTGAAGCTGGAGATAATATTGCCTCAGTCTCAAC
TTTATATGGTGGAACGTACAACTTATTTGCTCATACCCTGCCAAAACAAGGCATTGAAGTTCGCTTTTTTGATTATCAAA
AGCCTAAAAGCTTACGTAACTTAATTGATGATAAAACTAAACTTGTTTTTGTTGAGTCAATCGGCAATCCGCTTGGAAAT
ATCATCGATCTAGAAGAAATTTCGAAAATCGCCCATGAATATGGCGTACCTGTCGTTGTTGATAATACAGTCGCTACCCC
TGCTTTATTAAAACCATTCCAATATGGCGCCGATATCGTCATACATTCTTTGACTAAATATATTGGTGGTCATGGCAATA
GTATTGGTGGTGCGATTATCGATAGCGGTAAGTTTCCTTGGGGTAAATATCCGGAGCGTTTTAAAGTATTGAACACGCCA
GACCCAAGCTATCATGGTGTGAACTATGTCGAGGCATTAGGTGAAGCTGCCTATATAGCAAGGGCTCGCGTTGTACCATT
ACGTAATACAGGCGCAGCTATTAGTCCGCTCAGTGTTTTCTTAATTTTACAGGGTCTAGAAACGCTTAATTTACGGATGG
AACGCCATACTGAAAATGCACAAAGAATTGCGGAATATTTGCAAAATCATCCGAAAGTAAAATGGGTCAATTACGCAGGC
TTAAAAGATCATCCACAACACCAACTTGCGCAAAAATATGTAAAAGGTAAACCTTCTGCAATTTTATCTTTTGGGGTTCA
AGATGGCCGTGAAGGCGGTACTCGCTTCATTGATGCCCTCCAGCTATTTACCCGCCTTGTGAACATTGGTGATGCGAAAA
GTCTGGCGTGTTACCCTGCAACCACAACTCACCGCCAACTCAACGAACAAGAACTAAAAGCTGCTGGTGTAAGTGAAGAT
ATGGTACGTCTTTCAATTGGTATCGAGCATGTTGATGACCTCATTGCCGATTTAGAACAAGCACTTTCATCCGTATAA

Upstream 100 bases:

>100_bases
ATATTCCTAAAATTGGATAACAAACCAGAATTTCTGCAATTTTCTATATAAATTTTAAGCTAGACTCGCTCAATATTTAA
CTCACGAAGATGAAGTAATC

Downstream 100 bases:

>100_bases
ATTTTAAACTCATCTAACAAGCCTCTGGAATATAGAGGTCTTTTTTATTTTCAAGTGATTTAACTATTAAGAATAATTAA
TGGATTAGGGTAGTAATTTA

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTYKDETLAIHAGYTPEATTKAVAVPIYQTTSYAFDNTQHGADLFDLKVQGNIYTRIMNPTTAVLEQRLAALEGGIGALA
LASGMAAITYAIQTITEAGDNIASVSTLYGGTYNLFAHTLPKQGIEVRFFDYQKPKSLRNLIDDKTKLVFVESIGNPLGN
IIDLEEISKIAHEYGVPVVVDNTVATPALLKPFQYGADIVIHSLTKYIGGHGNSIGGAIIDSGKFPWGKYPERFKVLNTP
DPSYHGVNYVEALGEAAYIARARVVPLRNTGAAISPLSVFLILQGLETLNLRMERHTENAQRIAEYLQNHPKVKWVNYAG
LKDHPQHQLAQKYVKGKPSAILSFGVQDGREGGTRFIDALQLFTRLVNIGDAKSLACYPATTTHRQLNEQELKAAGVSED
MVRLSIGIEHVDDLIADLEQALSSV

Sequences:

>Translated_425_residues
MTYKDETLAIHAGYTPEATTKAVAVPIYQTTSYAFDNTQHGADLFDLKVQGNIYTRIMNPTTAVLEQRLAALEGGIGALA
LASGMAAITYAIQTITEAGDNIASVSTLYGGTYNLFAHTLPKQGIEVRFFDYQKPKSLRNLIDDKTKLVFVESIGNPLGN
IIDLEEISKIAHEYGVPVVVDNTVATPALLKPFQYGADIVIHSLTKYIGGHGNSIGGAIIDSGKFPWGKYPERFKVLNTP
DPSYHGVNYVEALGEAAYIARARVVPLRNTGAAISPLSVFLILQGLETLNLRMERHTENAQRIAEYLQNHPKVKWVNYAG
LKDHPQHQLAQKYVKGKPSAILSFGVQDGREGGTRFIDALQLFTRLVNIGDAKSLACYPATTTHRQLNEQELKAAGVSED
MVRLSIGIEHVDDLIADLEQALSSV
>Mature_424_residues
TYKDETLAIHAGYTPEATTKAVAVPIYQTTSYAFDNTQHGADLFDLKVQGNIYTRIMNPTTAVLEQRLAALEGGIGALAL
ASGMAAITYAIQTITEAGDNIASVSTLYGGTYNLFAHTLPKQGIEVRFFDYQKPKSLRNLIDDKTKLVFVESIGNPLGNI
IDLEEISKIAHEYGVPVVVDNTVATPALLKPFQYGADIVIHSLTKYIGGHGNSIGGAIIDSGKFPWGKYPERFKVLNTPD
PSYHGVNYVEALGEAAYIARARVVPLRNTGAAISPLSVFLILQGLETLNLRMERHTENAQRIAEYLQNHPKVKWVNYAGL
KDHPQHQLAQKYVKGKPSAILSFGVQDGREGGTRFIDALQLFTRLVNIGDAKSLACYPATTTHRQLNEQELKAAGVSEDM
VRLSIGIEHVDDLIADLEQALSSV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=421, Percent_Identity=34.6793349168646, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI299473759, Length=421, Percent_Identity=31.1163895486936, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI25453487, Length=420, Percent_Identity=30.7142857142857, Blast_Score=155, Evalue=5e-38,
Organism=Escherichia coli, GI1790375, Length=425, Percent_Identity=34.5882352941176, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI1789383, Length=429, Percent_Identity=26.3403263403263, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71980708, Length=420, Percent_Identity=31.1904761904762, Blast_Score=214, Evalue=9e-56,
Organism=Caenorhabditis elegans, GI17538055, Length=421, Percent_Identity=33.729216152019, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17560056, Length=420, Percent_Identity=34.2857142857143, Blast_Score=194, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6323333, Length=433, Percent_Identity=48.729792147806, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6319307, Length=375, Percent_Identity=31.7333333333333, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321254, Length=442, Percent_Identity=27.8280542986425, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6321170, Length=355, Percent_Identity=26.4788732394366, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6321904, Length=419, Percent_Identity=22.1957040572792, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922554, Length=422, Percent_Identity=33.8862559241706, Blast_Score=213, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46410; Mature: 46279

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYKDETLAIHAGYTPEATTKAVAVPIYQTTSYAFDNTQHGADLFDLKVQGNIYTRIMNP
CCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEECCH
TTAVLEQRLAALEGGIGALALASGMAAITYAIQTITEAGDNIASVSTLYGGTYNLFAHTL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHC
PKQGIEVRFFDYQKPKSLRNLIDDKTKLVFVESIGNPLGNIIDLEEISKIAHEYGVPVVV
CCCCCEEEEECCCCCHHHHHHHCCCCEEEEEHHHCCCHHCEECHHHHHHHHHHCCCCEEE
DNTVATPALLKPFQYGADIVIHSLTKYIGGHGNSIGGAIIDSGKFPWGKYPERFKVLNTP
CCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEEEECCC
DPSYHGVNYVEALGEAAYIARARVVPLRNTGAAISPLSVFLILQGLETLNLRMERHTENA
CCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRIAEYLQNHPKVKWVNYAGLKDHPQHQLAQKYVKGKPSAILSFGVQDGREGGTRFIDAL
HHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHH
QLFTRLVNIGDAKSLACYPATTTHRQLNEQELKAAGVSEDMVRLSIGIEHVDDLIADLEQ
HHHHHHHCCCCCCCEEEECCCCHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ALSSV
HHHCC
>Mature Secondary Structure 
TYKDETLAIHAGYTPEATTKAVAVPIYQTTSYAFDNTQHGADLFDLKVQGNIYTRIMNP
CCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEECCH
TTAVLEQRLAALEGGIGALALASGMAAITYAIQTITEAGDNIASVSTLYGGTYNLFAHTL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHC
PKQGIEVRFFDYQKPKSLRNLIDDKTKLVFVESIGNPLGNIIDLEEISKIAHEYGVPVVV
CCCCCEEEEECCCCCHHHHHHHCCCCEEEEEHHHCCCHHCEECHHHHHHHHHHCCCCEEE
DNTVATPALLKPFQYGADIVIHSLTKYIGGHGNSIGGAIIDSGKFPWGKYPERFKVLNTP
CCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEEEECCC
DPSYHGVNYVEALGEAAYIARARVVPLRNTGAAISPLSVFLILQGLETLNLRMERHTENA
CCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRIAEYLQNHPKVKWVNYAGLKDHPQHQLAQKYVKGKPSAILSFGVQDGREGGTRFIDAL
HHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHH
QLFTRLVNIGDAKSLACYPATTTHRQLNEQELKAAGVSEDMVRLSIGIEHVDDLIADLEQ
HHHHHHHCCCCCCCEEEECCCCHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ALSSV
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]