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The map label for this gene is gltX [H]

Identifier: 213158959

GI number: 213158959

Start: 3771357

End: 3772865

Strand: Direct

Name: gltX [H]

Synonym: AB57_3660

Alternate gene names: 213158959

Gene position: 3771357-3772865 (Clockwise)

Preceding gene: 213158958

Following gene: 213158960

Centisome position: 93.11

GC content: 40.95

Gene sequence:

>1509_bases
ATGAAAGTTCGTACTCGTATTGCACCTTCTCCTACCGGTTTCCCACATGTTGGTACTGCCTACATCGCCCTATTCAATAT
GTGTTTTGCGAAACAGCATGGCGGGGAATTCATTTTAAGAATTGAAGATACAGACCAGCTTCGCTCTACGCCAGAATCTG
AAAAAATGATTTTAGACTCTCTACGCTGGTTAGGCTTAAATTGGTCTGAAGGTCCGGATGTAGGTGGTCCTCACGCTCCT
TACCGCCAATCAGAGCGCATGGGAATTTATAAGCAATACGCGTTAGAACTTGTTGAAAAAGGTCATGCTTTTTATTGTTT
TGCTACAGCAGAAGAACTTGACCAAATGCGTGCCGAGCAGCAAGCTCGTGGCGAAACACCAAAATACGATGGTCGTGGTT
TAAAGCTGTCTCAAGAAGAAGTAGCTCGACGTCTTGAAGCGGGTGAACCTCATGTTATCCGTATGAAAGTGCCTGAAGAA
GGCGTATGTAAATTTAACGACTTACTCCGTGGGGAAGTTGAAATTCCTTGGGCACAAGTCGATATGCAAGTGCTTCTTAA
AACAGATGGTTTACCAACCTACCATTTAGCTAACGTTGTTGATGACCATTTAATGGAAATCACTCATGTATTACGTGGTG
AAGAATGGCTTCCATCTGCACCGAAGCATCAATTGCTTTACCAATACTTTGGTTGGGAAATGCCAACGCTTTGCCATATG
CCGTTGCTTCGTAATCCAGATAAATCGAAGTTATCTAAACGCAAAAACCCGACCTCAATTAACTACTACCGTGATATCGG
CGTTTTACCAGAAGCCTTACTCAACTATTTAGGCCGTATGGGTTGGTCAATGCCGGATGAAAGAGAAGTTTTCACATTAC
AAGACATGATGGATAACTTTGATATTCAACGCGTATCACTAGGTGGACCAATTTTTGACGTTGAAAAACTCAATTGGTTA
AATGGTCAATGGATTAAAGGGTTAACCCCAGGACAACTATTAGATCGTTTATTGACATGGAAAAGTGATCGCAGCACTTT
AGAAGACATTGCTGCCGCTATTCAACCACGTATTAATTTACTTTCTGAAGCTGTAAATTGGGCTGGTTTCTACTTTAACC
ATATGCCACAAATTACGGCTGAAATGTTTGAAAGTAAAAAATTGACTCAAGAGCAAGTTCGTCAAAGTTTACAATTCGCA
ATTTGGCGTCTTGAAAGTCAGTTTACTTGGAATAACGACACAGTTAGTCAAACTTTAATGGATTTGGCAAATCAGATGGG
CATTAAATTACGTGACTTCATGCCAACGTTCTTTATTGCAATTGCTGGCTCAACAAGTTCAACACCAGTTATGCAGTCAA
TGGTTACGCTTGGCCCTGACTTAACCTTTGCTCGCTTACGACATGCACTAGAAATTGTAGGTGCACCGAGTAAAAAAGAG
GTAAAAAACTGGGAAAAGCTTAATGAAAGTTTAAAATTGCCGAAAAATGAAGCAACTAGCGAAGCTTAA

Upstream 100 bases:

>100_bases
TCATCGTTTCAATTTCAGCCCAGACTGTCGTTAAATAAAACGTCAGAATACTGGGCTTTTTCGTTATAATAGTTCCAATT
TTTATTTTGACCCATTAACT

Downstream 100 bases:

>100_bases
TTCATTTTTTTATTGACGTGTGAGCGCATTCACCCTAATATTGCGCTCACACAAACTGGGGTCATAGCTCAGTTGGTAGA
GCGCTACAATGGCATTGTAG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MKVRTRIAPSPTGFPHVGTAYIALFNMCFAKQHGGEFILRIEDTDQLRSTPESEKMILDSLRWLGLNWSEGPDVGGPHAP
YRQSERMGIYKQYALELVEKGHAFYCFATAEELDQMRAEQQARGETPKYDGRGLKLSQEEVARRLEAGEPHVIRMKVPEE
GVCKFNDLLRGEVEIPWAQVDMQVLLKTDGLPTYHLANVVDDHLMEITHVLRGEEWLPSAPKHQLLYQYFGWEMPTLCHM
PLLRNPDKSKLSKRKNPTSINYYRDIGVLPEALLNYLGRMGWSMPDEREVFTLQDMMDNFDIQRVSLGGPIFDVEKLNWL
NGQWIKGLTPGQLLDRLLTWKSDRSTLEDIAAAIQPRINLLSEAVNWAGFYFNHMPQITAEMFESKKLTQEQVRQSLQFA
IWRLESQFTWNNDTVSQTLMDLANQMGIKLRDFMPTFFIAIAGSTSSTPVMQSMVTLGPDLTFARLRHALEIVGAPSKKE
VKNWEKLNESLKLPKNEATSEA

Sequences:

>Translated_502_residues
MKVRTRIAPSPTGFPHVGTAYIALFNMCFAKQHGGEFILRIEDTDQLRSTPESEKMILDSLRWLGLNWSEGPDVGGPHAP
YRQSERMGIYKQYALELVEKGHAFYCFATAEELDQMRAEQQARGETPKYDGRGLKLSQEEVARRLEAGEPHVIRMKVPEE
GVCKFNDLLRGEVEIPWAQVDMQVLLKTDGLPTYHLANVVDDHLMEITHVLRGEEWLPSAPKHQLLYQYFGWEMPTLCHM
PLLRNPDKSKLSKRKNPTSINYYRDIGVLPEALLNYLGRMGWSMPDEREVFTLQDMMDNFDIQRVSLGGPIFDVEKLNWL
NGQWIKGLTPGQLLDRLLTWKSDRSTLEDIAAAIQPRINLLSEAVNWAGFYFNHMPQITAEMFESKKLTQEQVRQSLQFA
IWRLESQFTWNNDTVSQTLMDLANQMGIKLRDFMPTFFIAIAGSTSSTPVMQSMVTLGPDLTFARLRHALEIVGAPSKKE
VKNWEKLNESLKLPKNEATSEA
>Mature_502_residues
MKVRTRIAPSPTGFPHVGTAYIALFNMCFAKQHGGEFILRIEDTDQLRSTPESEKMILDSLRWLGLNWSEGPDVGGPHAP
YRQSERMGIYKQYALELVEKGHAFYCFATAEELDQMRAEQQARGETPKYDGRGLKLSQEEVARRLEAGEPHVIRMKVPEE
GVCKFNDLLRGEVEIPWAQVDMQVLLKTDGLPTYHLANVVDDHLMEITHVLRGEEWLPSAPKHQLLYQYFGWEMPTLCHM
PLLRNPDKSKLSKRKNPTSINYYRDIGVLPEALLNYLGRMGWSMPDEREVFTLQDMMDNFDIQRVSLGGPIFDVEKLNWL
NGQWIKGLTPGQLLDRLLTWKSDRSTLEDIAAAIQPRINLLSEAVNWAGFYFNHMPQITAEMFESKKLTQEQVRQSLQFA
IWRLESQFTWNNDTVSQTLMDLANQMGIKLRDFMPTFFIAIAGSTSSTPVMQSMVTLGPDLTFARLRHALEIVGAPSKKE
VKNWEKLNESLKLPKNEATSEA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=491, Percent_Identity=34.826883910387, Blast_Score=270, Evalue=3e-72,
Organism=Homo sapiens, GI62241042, Length=259, Percent_Identity=25.4826254826255, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1788743, Length=482, Percent_Identity=34.6473029045643, Blast_Score=301, Evalue=5e-83,
Organism=Escherichia coli, GI226510925, Length=259, Percent_Identity=33.5907335907336, Blast_Score=118, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI71993394, Length=481, Percent_Identity=33.8877338877339, Blast_Score=263, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6324540, Length=373, Percent_Identity=39.142091152815, Blast_Score=256, Evalue=4e-69,
Organism=Saccharomyces cerevisiae, GI6324742, Length=304, Percent_Identity=24.6710526315789, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321192, Length=231, Percent_Identity=24.2424242424242, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24665321, Length=494, Percent_Identity=33.8056680161943, Blast_Score=271, Evalue=6e-73,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 57492; Mature: 57492

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00132 CARBOXYPEPT_ZN_1 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRTRIAPSPTGFPHVGTAYIALFNMCFAKQHGGEFILRIEDTDQLRSTPESEKMILDS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHHHH
LRWLGLNWSEGPDVGGPHAPYRQSERMGIYKQYALELVEKGHAFYCFATAEELDQMRAEQ
HHHCCCCCCCCCCCCCCCCCCCCHHHCHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHH
QARGETPKYDGRGLKLSQEEVARRLEAGEPHVIRMKVPEEGVCKFNDLLRGEVEIPWAQV
HHCCCCCCCCCCCCEECHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCCCCCHHHH
DMQVLLKTDGLPTYHLANVVDDHLMEITHVLRGEEWLPSAPKHQLLYQYFGWEMPTLCHM
HHHHEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHCC
PLLRNPDKSKLSKRKNPTSINYYRDIGVLPEALLNYLGRMGWSMPDEREVFTLQDMMDNF
HHHCCCCHHHHHHCCCCCCCCHHHHHCHHHHHHHHHHHHHCCCCCCCCHHEEHHHHHCCC
DIQRVSLGGPIFDVEKLNWLNGQWIKGLTPGQLLDRLLTWKSDRSTLEDIAAAIQPRINL
CEEEEECCCCEEEHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LSEAVNWAGFYFNHMPQITAEMFESKKLTQEQVRQSLQFAIWRLESQFTWNNDTVSQTLM
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCEECCCHHHHHHHH
DLANQMGIKLRDFMPTFFIAIAGSTSSTPVMQSMVTLGPDLTFARLRHALEIVGAPSKKE
HHHHHCCCCHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHH
VKNWEKLNESLKLPKNEATSEA
HHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKVRTRIAPSPTGFPHVGTAYIALFNMCFAKQHGGEFILRIEDTDQLRSTPESEKMILDS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHHHH
LRWLGLNWSEGPDVGGPHAPYRQSERMGIYKQYALELVEKGHAFYCFATAEELDQMRAEQ
HHHCCCCCCCCCCCCCCCCCCCCHHHCHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHH
QARGETPKYDGRGLKLSQEEVARRLEAGEPHVIRMKVPEEGVCKFNDLLRGEVEIPWAQV
HHCCCCCCCCCCCCEECHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCCCCCHHHH
DMQVLLKTDGLPTYHLANVVDDHLMEITHVLRGEEWLPSAPKHQLLYQYFGWEMPTLCHM
HHHHEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHCC
PLLRNPDKSKLSKRKNPTSINYYRDIGVLPEALLNYLGRMGWSMPDEREVFTLQDMMDNF
HHHCCCCHHHHHHCCCCCCCCHHHHHCHHHHHHHHHHHHHCCCCCCCCHHEEHHHHHCCC
DIQRVSLGGPIFDVEKLNWLNGQWIKGLTPGQLLDRLLTWKSDRSTLEDIAAAIQPRINL
CEEEEECCCCEEEHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LSEAVNWAGFYFNHMPQITAEMFESKKLTQEQVRQSLQFAIWRLESQFTWNNDTVSQTLM
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCEECCCHHHHHHHH
DLANQMGIKLRDFMPTFFIAIAGSTSSTPVMQSMVTLGPDLTFARLRHALEIVGAPSKKE
HHHHHCCCCHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHH
VKNWEKLNESLKLPKNEATSEA
HHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA