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The map label for this gene is sbp [H]

Identifier: 213158957

GI number: 213158957

Start: 3769545

End: 3770546

Strand: Reverse

Name: sbp [H]

Synonym: AB57_3658

Alternate gene names: 213158957

Gene position: 3770546-3769545 (Counterclockwise)

Preceding gene: 213158962

Following gene: 213158956

Centisome position: 93.09

GC content: 40.52

Gene sequence:

>1002_bases
ATGAAAAGAATAGCAATAATTGCAGTCGCTACATATATGGGGTTGGCAGTAAGTGCTGTGCAGGCGGCGACTACATTTTT
AAATGTTTCTTATGACCCAACTCGTGAGTTTTATCAGGAATATAATCAGGCATTTGGTAAGTTCTGGAAACAACGTACCG
GGCAGGACGTGGATTTTAAACAATCCCATGGAGGCTCAGGTAAACAGGCACGCGCTGTAGCAACTGGTCTTGAGGCAGAT
GTGGTGACCTTAGCCTTAGCCAATGATATTGATGAAATTGTAAAAGCAGGCTTTATCCAGCCAAACTGGCAAAAAGAATT
CCCTAATAATTCTGCACCGTATACCTCTACAGTTGTGTTTTTAGTAAGAAAAGGCAATCCAAAAAATATTCGTGATTGGA
ATGATTTAACCAAACCTGGTGTTGAAATTATTACACCTAATCCGAAAACGGGTGGAGCACCGCGTTGGATTTATCTTTCT
GCATGGGGATATGCTTTAAAGCAGCCGGGTGGAAATGACGCTAAAGCCAAAGAGCTGGTTAAAAAACTTTATCACAACGT
TAAGGTGTTAGATTCGGGAGCGCGAGGCTCGCTTACTACATTTGCTGAGCGTGGAATTGGAGATGTTCTACTTTCTTGGG
AGAACGAAGCACTATTGGCAACGAAAGGTCTCGATAAAGATAAATATGAAATTGTTTACCCATCAATTTCAATTTTGGCA
GAACCATCTGTTGCCATTGTTGACAAAACAGTCGACAAAGACGGTAACCGTACTTTGGCAAAAGGTTATCTCAACTTTTT
ATACTCACCTTTAGGTCAAGAGTTGGCTGCTAAACACTATTTCCGTCCTCGTAATCCTCAAGTTGCTGCTAAATATGCAG
CACAATTCCCAAAAATTAAATTATTTACGATCAATGATGTTTTTGGTGGTTGGGCAAAAGCTCAGAAAACCCATTTTGCC
AATGGAGCTATCTTTGATCAGATTTATGATGTAAAGCAGTAG

Upstream 100 bases:

>100_bases
TTTAAAGTGATTAGTAAAACATTTATTTGTATAACTATTTGATTGAAAACTAAAAAAATAAAAATATAGTAGACGTTAAA
AGTAAAATTGGTTTTTATAA

Downstream 100 bases:

>100_bases
ATAAAATTTTGGTAGAGTAGAAGTTTAAAAATTAGTTTACTTTTTTATAGTGAAGCAAGTGAGCCTGAAAAGGTGAGCTT
GCTTTTTTGTTTTGCTTAAA

Product: thiosulfate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKRIAIIAVATYMGLAVSAVQAATTFLNVSYDPTREFYQEYNQAFGKFWKQRTGQDVDFKQSHGGSGKQARAVATGLEAD
VVTLALANDIDEIVKAGFIQPNWQKEFPNNSAPYTSTVVFLVRKGNPKNIRDWNDLTKPGVEIITPNPKTGGAPRWIYLS
AWGYALKQPGGNDAKAKELVKKLYHNVKVLDSGARGSLTTFAERGIGDVLLSWENEALLATKGLDKDKYEIVYPSISILA
EPSVAIVDKTVDKDGNRTLAKGYLNFLYSPLGQELAAKHYFRPRNPQVAAKYAAQFPKIKLFTINDVFGGWAKAQKTHFA
NGAIFDQIYDVKQ

Sequences:

>Translated_333_residues
MKRIAIIAVATYMGLAVSAVQAATTFLNVSYDPTREFYQEYNQAFGKFWKQRTGQDVDFKQSHGGSGKQARAVATGLEAD
VVTLALANDIDEIVKAGFIQPNWQKEFPNNSAPYTSTVVFLVRKGNPKNIRDWNDLTKPGVEIITPNPKTGGAPRWIYLS
AWGYALKQPGGNDAKAKELVKKLYHNVKVLDSGARGSLTTFAERGIGDVLLSWENEALLATKGLDKDKYEIVYPSISILA
EPSVAIVDKTVDKDGNRTLAKGYLNFLYSPLGQELAAKHYFRPRNPQVAAKYAAQFPKIKLFTINDVFGGWAKAQKTHFA
NGAIFDQIYDVKQ
>Mature_333_residues
MKRIAIIAVATYMGLAVSAVQAATTFLNVSYDPTREFYQEYNQAFGKFWKQRTGQDVDFKQSHGGSGKQARAVATGLEAD
VVTLALANDIDEIVKAGFIQPNWQKEFPNNSAPYTSTVVFLVRKGNPKNIRDWNDLTKPGVEIITPNPKTGGAPRWIYLS
AWGYALKQPGGNDAKAKELVKKLYHNVKVLDSGARGSLTTFAERGIGDVLLSWENEALLATKGLDKDKYEIVYPSISILA
EPSVAIVDKTVDKDGNRTLAKGYLNFLYSPLGQELAAKHYFRPRNPQVAAKYAAQFPKIKLFTINDVFGGWAKAQKTHFA
NGAIFDQIYDVKQ

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=318, Percent_Identity=63.5220125786163, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1788765, Length=308, Percent_Identity=46.7532467532467, Blast_Score=283, Evalue=1e-77,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36860; Mature: 36860

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIAIIAVATYMGLAVSAVQAATTFLNVSYDPTREFYQEYNQAFGKFWKQRTGQDVDFK
CCEEEEEHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCH
QSHGGSGKQARAVATGLEADVVTLALANDIDEIVKAGFIQPNWQKEFPNNSAPYTSTVVF
HCCCCCCCHHHHHHCCCCHHHEEHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCEEEEE
LVRKGNPKNIRDWNDLTKPGVEIITPNPKTGGAPRWIYLSAWGYALKQPGGNDAKAKELV
EEECCCCCCCCCCHHHCCCCCEEECCCCCCCCCCCEEEEEECCHHHCCCCCCCHHHHHHH
KKLYHNVKVLDSGARGSLTTFAERGIGDVLLSWENEALLATKGLDKDKYEIVYPSISILA
HHHHCCEEEEECCCCCCHHHHHHCCCCCEEEECCCCEEEEECCCCCCCEEEECCCCEEEE
EPSVAIVDKTVDKDGNRTLAKGYLNFLYSPLGQELAAKHYFRPRNPQVAAKYAAQFPKIK
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEE
LFTINDVFGGWAKAQKTHFANGAIFDQIYDVKQ
EEEECCHHCCHHHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure
MKRIAIIAVATYMGLAVSAVQAATTFLNVSYDPTREFYQEYNQAFGKFWKQRTGQDVDFK
CCEEEEEHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCH
QSHGGSGKQARAVATGLEADVVTLALANDIDEIVKAGFIQPNWQKEFPNNSAPYTSTVVF
HCCCCCCCHHHHHHCCCCHHHEEHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCEEEEE
LVRKGNPKNIRDWNDLTKPGVEIITPNPKTGGAPRWIYLSAWGYALKQPGGNDAKAKELV
EEECCCCCCCCCCHHHCCCCCEEECCCCCCCCCCCEEEEEECCHHHCCCCCCCHHHHHHH
KKLYHNVKVLDSGARGSLTTFAERGIGDVLLSWENEALLATKGLDKDKYEIVYPSISILA
HHHHCCEEEEECCCCCCHHHHHHCCCCCEEEECCCCEEEEECCCCCCCEEEECCCCEEEE
EPSVAIVDKTVDKDGNRTLAKGYLNFLYSPLGQELAAKHYFRPRNPQVAAKYAAQFPKIK
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEE
LFTINDVFGGWAKAQKTHFANGAIFDQIYDVKQ
EEEECCHHCCHHHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]