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The map label for this gene is mraW [H]

Identifier: 213158956

GI number: 213158956

Start: 3768399

End: 3769322

Strand: Reverse

Name: mraW [H]

Synonym: AB57_3657

Alternate gene names: 213158956

Gene position: 3769322-3768399 (Counterclockwise)

Preceding gene: 213158957

Following gene: 213158955

Centisome position: 93.06

GC content: 42.75

Gene sequence:

>924_bases
ATGTCGCATATTTCTGTCTTACTTTTTGAGACCGTTGAAAGCTTATTGGCAGATCGCACAACTGGGGTGTATATCGATGC
AACCTTTGGACGAGGTGGGCATACGCGCTTGTTATTGTCGAAACTCGATGAAAACGCCCGAGTATATGCTTTTGATAAAG
ATCCTCAAGCTCTGGAAGTTGCGGCTGCTTTAGCACAAGAAGATCCAAGATTTACGATTATTCATGCAAGTTTTGCTGAT
ATTAAGGAAAAAATGCAGGAAATTGGGGTGCAAAGTGTCGATGGAATTATGGCAGACTTAGGTGTGTCCTCTCCTCAACT
CGACCAGGCTGAACGCGGCTTTAGCTTTATGCAAGATGGCCCGTTAGATATGCGCATGGATAACTCTAAAGGATTGACTG
CTGACGAATGGTTACTTGAGGTAGAAGAGGAAGATTTGGCAAATATCATTTATCAATATGGTGAAGAACGATATAGTCGC
CGTATTGCTAGAGCAATTAAACAGGCCGGAAAATTGGATACTACGGCGCAATTGGCTGAAATAGTAAAAACAGCCCATCC
AAAATGGGAAAAGCATAAACATCCGGCGACACGTACGTTTCAGGCAATCCGTATTGCTATTAACAAAGAACTAGATGATA
TTGAAGTATTTTTACCGCAAGCTGTAGACTTGCTTAAACCAAAAGGGCGTTTGTCTGTGATCAGCTTCCATTCGCTTGAA
GATCGGTTGATCAAACAGTTTATTCAAAAAGAATCAACTCTAGCAGAAGATTCTGGTTGGGGGATGCCTCAACAGCAGGT
TGATACGAGAAGATTAAAGAAAATTTCACGGGTTCGTGCGAGTGAAGAAGAAGTAAAAGCGAATCCACGTTCACGTAGTG
CATGGCTTCGGGTTGCTGAACGCTTGGAACAAAAAGGCGCGTAA

Upstream 100 bases:

>100_bases
TCTATGAAAAATTAATATTTTCCTACAAAAAGCCGTTTGGTGTTAGCCATAAACAAGGTGATAATGTGCAGTTACATTTT
TACGCTATGATTTAACTCCT

Downstream 100 bases:

>100_bases
TGAAAAGCAGTGATGAAATCGAAACCACAGAAAATAAAGTGGTCAAAAAAGTTGTGGTATATGCAGTCATGGTGGCATTG
GTTTTTATCAGTGCCATGAT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSHISVLLFETVESLLADRTTGVYIDATFGRGGHTRLLLSKLDENARVYAFDKDPQALEVAAALAQEDPRFTIIHASFAD
IKEKMQEIGVQSVDGIMADLGVSSPQLDQAERGFSFMQDGPLDMRMDNSKGLTADEWLLEVEEEDLANIIYQYGEERYSR
RIARAIKQAGKLDTTAQLAEIVKTAHPKWEKHKHPATRTFQAIRIAINKELDDIEVFLPQAVDLLKPKGRLSVISFHSLE
DRLIKQFIQKESTLAEDSGWGMPQQQVDTRRLKKISRVRASEEEVKANPRSRSAWLRVAERLEQKGA

Sequences:

>Translated_307_residues
MSHISVLLFETVESLLADRTTGVYIDATFGRGGHTRLLLSKLDENARVYAFDKDPQALEVAAALAQEDPRFTIIHASFAD
IKEKMQEIGVQSVDGIMADLGVSSPQLDQAERGFSFMQDGPLDMRMDNSKGLTADEWLLEVEEEDLANIIYQYGEERYSR
RIARAIKQAGKLDTTAQLAEIVKTAHPKWEKHKHPATRTFQAIRIAINKELDDIEVFLPQAVDLLKPKGRLSVISFHSLE
DRLIKQFIQKESTLAEDSGWGMPQQQVDTRRLKKISRVRASEEEVKANPRSRSAWLRVAERLEQKGA
>Mature_306_residues
SHISVLLFETVESLLADRTTGVYIDATFGRGGHTRLLLSKLDENARVYAFDKDPQALEVAAALAQEDPRFTIIHASFADI
KEKMQEIGVQSVDGIMADLGVSSPQLDQAERGFSFMQDGPLDMRMDNSKGLTADEWLLEVEEEDLANIIYQYGEERYSRR
IARAIKQAGKLDTTAQLAEIVKTAHPKWEKHKHPATRTFQAIRIAINKELDDIEVFLPQAVDLLKPKGRLSVISFHSLED
RLIKQFIQKESTLAEDSGWGMPQQQVDTRRLKKISRVRASEEEVKANPRSRSAWLRVAERLEQKGA

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=339, Percent_Identity=36.8731563421829, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI165377202, Length=189, Percent_Identity=39.1534391534392, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1786270, Length=307, Percent_Identity=51.1400651465798, Blast_Score=301, Evalue=4e-83,
Organism=Drosophila melanogaster, GI62472493, Length=328, Percent_Identity=39.3292682926829, Blast_Score=194, Evalue=5e-50,
Organism=Drosophila melanogaster, GI28571637, Length=328, Percent_Identity=39.3292682926829, Blast_Score=194, Evalue=5e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 34715; Mature: 34584

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHISVLLFETVESLLADRTTGVYIDATFGRGGHTRLLLSKLDENARVYAFDKDPQALEV
CCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHH
AAALAQEDPRFTIIHASFADIKEKMQEIGVQSVDGIMADLGVSSPQLDQAERGFSFMQDG
HHHHHCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHCCHHHCCC
PLDMRMDNSKGLTADEWLLEVEEEDLANIIYQYGEERYSRRIARAIKQAGKLDTTAQLAE
CCCEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
IVKTAHPKWEKHKHPATRTFQAIRIAINKELDDIEVFLPQAVDLLKPKGRLSVISFHSLE
HHHHCCCCHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHH
DRLIKQFIQKESTLAEDSGWGMPQQQVDTRRLKKISRVRASEEEVKANPRSRSAWLRVAE
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHH
RLEQKGA
HHHHCCC
>Mature Secondary Structure 
SHISVLLFETVESLLADRTTGVYIDATFGRGGHTRLLLSKLDENARVYAFDKDPQALEV
CCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHH
AAALAQEDPRFTIIHASFADIKEKMQEIGVQSVDGIMADLGVSSPQLDQAERGFSFMQDG
HHHHHCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHCCHHHCCC
PLDMRMDNSKGLTADEWLLEVEEEDLANIIYQYGEERYSRRIARAIKQAGKLDTTAQLAE
CCCEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
IVKTAHPKWEKHKHPATRTFQAIRIAINKELDDIEVFLPQAVDLLKPKGRLSVISFHSLE
HHHHCCCCHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHH
DRLIKQFIQKESTLAEDSGWGMPQQQVDTRRLKKISRVRASEEEVKANPRSRSAWLRVAE
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHH
RLEQKGA
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA