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The map label for this gene is murE [H]

Identifier: 213158953

GI number: 213158953

Start: 3764720

End: 3766219

Strand: Reverse

Name: murE [H]

Synonym: AB57_3654

Alternate gene names: 213158953

Gene position: 3766219-3764720 (Counterclockwise)

Preceding gene: 213158954

Following gene: 213158952

Centisome position: 92.98

GC content: 41.33

Gene sequence:

>1500_bases
ATGACTGTTTCTTTTCAAGAAATTCATCCAATCGAAATTGATGCTCAATGGCCTCAACAGCCATTTCACGGCTTTAGTTT
AGATAGTCGTAAAGTTGAAACGGGCCAAATTTTTATTGCTTTGACAAGTTATAGCCAGCCTGAAAAAACGCGTACATTTG
CTGAAGCGGCTTTAGCAAATGGAGCGTTGGCTGTCATTAGCGAAACCGAATTAGGTGTAGCAAATGAATGGGTATGCTCT
GACGTGCGTCAGCGCATGGGAGACTGGCAAAAACGTTATTTGCAACAAGCTGATGTAGTAAAACCTCTACGGATTATTGC
TGTAACAGGCACAAATGGTAAAACAACAATTTCTCGTTTAATTGCTGAGTTAATTAGTTCACAACAGCAGCGCTGTGCAG
TGATGGGGACAACAGGGAATGGTATTTTACCGAATTTAACGCCTTCTACGCATACTACCCTCGACGCTCTACAGTTACAA
AATGCGCTACATGACTATGCTAAACAGGGAGCAACTTTTGCTTCTTTAGAAGCAAGTTCACATGGTCTTGAACAGGGGCG
ATTAAATGGTTGCGATATTGAAATTGCTGTTTATAGCAACTTAAGTCGTGACCATCTGGATTATCACGGCACTTTAGAAG
CTTATGCAGAAGCGAAGGCGCGTTTATTTCAGTTTAATTCGTTAAAAGTCGCTGTTATTAATTTAGATGATGCGCATGCT
GACTTGATGATTAAGTCTGCCCAAAATAATCCAGCACAACCTAAAATTTTGACTTACTCACTAACTCAAAATACAGCTGA
TTATTACATTACCGATTTAAGCTATAGCTTGGCGGGTGCAACTTTTAATTTAGTAAGTCAGCAAGGTTCATTTGCGGTAG
AAAGCCCATTATTAGGTCATTTCAATGTTGAAAATTTAATTGCCGCACTCATCGCTGCTGAGCAAGCTGGTTTCGATTTA
CAGGCATTGGTTAATTTTGTTCCTAAACTAATTGGTGCACCAGGCCGAATGCAAGTTATTCGTGATGATGAGCGATTATT
TGTAGTGGATTATGCGCATACCCCAGATGCATTGATTCAAGTGCTTAAAACATTAAAGCGTCATGTTTCGAATCAGCTTT
GGGCGGTGTTTGGATGTGGTGGTGACCGTGACCGTGGTAAGCGTCCACTCATGACTCAAGCAGCTTTGGATGGCGCAAAT
CCTGTTATTCTGACTTCAGATAATCCAAGAACAGAAGACCCTGAACAAATTTTTGCTGACATGAAGCAAGGAATCAATTT
TTCAGGGCATCGTATGCATGAAATTCATGACCGCCGTGAAGCAATTAAATTTGTTGCAGAGCAAGCTCAAGCTGGTGACA
TTGTAGTGATTGCCGGCAAAGGACATGAAAATTATCAAGAAATTAATGGTGTGCGTCACTGGTTTGATGATGTGGTTGAA
GTGCGTTCCGCGATTGATGCCCAACATCATACGGTAGATGCTGCTTACCCTGCACAATAG

Upstream 100 bases:

>100_bases
TCCAGTTTTTGCTCGTATTATGCAAGAGTCGTTGCGCTTAATGAATGTGCCGCTTGATAAGCCACTTAATACTCCAGAAA
ATCCTATTCGCAGGTAAGCT

Downstream 100 bases:

>100_bases
GAAAAATTATGCATACTTCAACCACCAGTACCGTGCCTTTGGAACCGTGGACAGCTCAACAATTACAACAGGCAACACAG
GGTTACTGGCATAAAGACCA

Product: UDP-N-acetylmuramyl-tripeptide synthetase

Products: ADP; phosphate; UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-; $heptanedioate

Alternate protein names: UDP-MurNAc-tripeptide synthetase [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MTVSFQEIHPIEIDAQWPQQPFHGFSLDSRKVETGQIFIALTSYSQPEKTRTFAEAALANGALAVISETELGVANEWVCS
DVRQRMGDWQKRYLQQADVVKPLRIIAVTGTNGKTTISRLIAELISSQQQRCAVMGTTGNGILPNLTPSTHTTLDALQLQ
NALHDYAKQGATFASLEASSHGLEQGRLNGCDIEIAVYSNLSRDHLDYHGTLEAYAEAKARLFQFNSLKVAVINLDDAHA
DLMIKSAQNNPAQPKILTYSLTQNTADYYITDLSYSLAGATFNLVSQQGSFAVESPLLGHFNVENLIAALIAAEQAGFDL
QALVNFVPKLIGAPGRMQVIRDDERLFVVDYAHTPDALIQVLKTLKRHVSNQLWAVFGCGGDRDRGKRPLMTQAALDGAN
PVILTSDNPRTEDPEQIFADMKQGINFSGHRMHEIHDRREAIKFVAEQAQAGDIVVIAGKGHENYQEINGVRHWFDDVVE
VRSAIDAQHHTVDAAYPAQ

Sequences:

>Translated_499_residues
MTVSFQEIHPIEIDAQWPQQPFHGFSLDSRKVETGQIFIALTSYSQPEKTRTFAEAALANGALAVISETELGVANEWVCS
DVRQRMGDWQKRYLQQADVVKPLRIIAVTGTNGKTTISRLIAELISSQQQRCAVMGTTGNGILPNLTPSTHTTLDALQLQ
NALHDYAKQGATFASLEASSHGLEQGRLNGCDIEIAVYSNLSRDHLDYHGTLEAYAEAKARLFQFNSLKVAVINLDDAHA
DLMIKSAQNNPAQPKILTYSLTQNTADYYITDLSYSLAGATFNLVSQQGSFAVESPLLGHFNVENLIAALIAAEQAGFDL
QALVNFVPKLIGAPGRMQVIRDDERLFVVDYAHTPDALIQVLKTLKRHVSNQLWAVFGCGGDRDRGKRPLMTQAALDGAN
PVILTSDNPRTEDPEQIFADMKQGINFSGHRMHEIHDRREAIKFVAEQAQAGDIVVIAGKGHENYQEINGVRHWFDDVVE
VRSAIDAQHHTVDAAYPAQ
>Mature_498_residues
TVSFQEIHPIEIDAQWPQQPFHGFSLDSRKVETGQIFIALTSYSQPEKTRTFAEAALANGALAVISETELGVANEWVCSD
VRQRMGDWQKRYLQQADVVKPLRIIAVTGTNGKTTISRLIAELISSQQQRCAVMGTTGNGILPNLTPSTHTTLDALQLQN
ALHDYAKQGATFASLEASSHGLEQGRLNGCDIEIAVYSNLSRDHLDYHGTLEAYAEAKARLFQFNSLKVAVINLDDAHAD
LMIKSAQNNPAQPKILTYSLTQNTADYYITDLSYSLAGATFNLVSQQGSFAVESPLLGHFNVENLIAALIAAEQAGFDLQ
ALVNFVPKLIGAPGRMQVIRDDERLFVVDYAHTPDALIQVLKTLKRHVSNQLWAVFGCGGDRDRGKRPLMTQAALDGANP
VILTSDNPRTEDPEQIFADMKQGINFSGHRMHEIHDRREAIKFVAEQAQAGDIVVIAGKGHENYQEINGVRHWFDDVVEV
RSAIDAQHHTVDAAYPAQ

Specific function: Catalyzes the addition of an amino acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=477, Percent_Identity=36.6876310272537, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1786274, Length=350, Percent_Identity=26, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.13

Molecular weight: Translated: 54982; Mature: 54851

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSFQEIHPIEIDAQWPQQPFHGFSLDSRKVETGQIFIALTSYSQPEKTRTFAEAALAN
CCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEECCEEEEEEECCCCCHHHHHHHHHHHCC
GALAVISETELGVANEWVCSDVRQRMGDWQKRYLQQADVVKPLRIIAVTGTNGKTTISRL
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHH
IAELISSQQQRCAVMGTTGNGILPNLTPSTHTTLDALQLQNALHDYAKQGATFASLEASS
HHHHHHCHHHCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCC
HGLEQGRLNGCDIEIAVYSNLSRDHLDYHGTLEAYAEAKARLFQFNSLKVAVINLDDAHA
CCCCCCCCCCCEEEEEEECCCCHHCCCCCCHHHHHHHHHHHHEEECCEEEEEEECCCCCC
DLMIKSAQNNPAQPKILTYSLTQNTADYYITDLSYSLAGATFNLVSQQGSFAVESPLLGH
CEEEEECCCCCCCCEEEEEEECCCCCCEEEEECCHHHCCCEEEEHHCCCCEEECCCCCCC
FNVENLIAALIAAEQAGFDLQALVNFVPKLIGAPGRMQVIRDDERLFVVDYAHTPDALIQ
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEECCCHHHHHH
VLKTLKRHVSNQLWAVFGCGGDRDRGKRPLMTQAALDGANPVILTSDNPRTEDPEQIFAD
HHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHH
MKQGINFSGHRMHEIHDRREAIKFVAEQAQAGDIVVIAGKGHENYQEINGVRHWFDDVVE
HHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
VRSAIDAQHHTVDAAYPAQ
HHHHHCHHHCEECCCCCCC
>Mature Secondary Structure 
TVSFQEIHPIEIDAQWPQQPFHGFSLDSRKVETGQIFIALTSYSQPEKTRTFAEAALAN
CCCCCCCCCEEECCCCCCCCCCCCCCCCCEEECCEEEEEEECCCCCHHHHHHHHHHHCC
GALAVISETELGVANEWVCSDVRQRMGDWQKRYLQQADVVKPLRIIAVTGTNGKTTISRL
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHH
IAELISSQQQRCAVMGTTGNGILPNLTPSTHTTLDALQLQNALHDYAKQGATFASLEASS
HHHHHHCHHHCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCC
HGLEQGRLNGCDIEIAVYSNLSRDHLDYHGTLEAYAEAKARLFQFNSLKVAVINLDDAHA
CCCCCCCCCCCEEEEEEECCCCHHCCCCCCHHHHHHHHHHHHEEECCEEEEEEECCCCCC
DLMIKSAQNNPAQPKILTYSLTQNTADYYITDLSYSLAGATFNLVSQQGSFAVESPLLGH
CEEEEECCCCCCCCEEEEEEECCCCCCEEEEECCHHHCCCEEEEHHCCCCEEECCCCCCC
FNVENLIAALIAAEQAGFDLQALVNFVPKLIGAPGRMQVIRDDERLFVVDYAHTPDALIQ
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEECCCHHHHHH
VLKTLKRHVSNQLWAVFGCGGDRDRGKRPLMTQAALDGANPVILTSDNPRTEDPEQIFAD
HHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHH
MKQGINFSGHRMHEIHDRREAIKFVAEQAQAGDIVVIAGKGHENYQEINGVRHWFDDVVE
HHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
VRSAIDAQHHTVDAAYPAQ
HHHHHCHHHCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {meso-2,6-diaminoheptanedioate}} 0.036 {meso-2,6-diaminoheptanedioate}} 1.5 {meso-lanthionine}} 10 {D-allo-cystathionine}} 3.9 {L-allo-cystathionine}} 0.62 {ATP}} 0.076 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.035 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.

Substrates: ATP; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate; meso-2,6-diaminoheptanedioate

Specific reaction: ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N- $acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino- $heptanedioate

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: MurNAc (alpha-methyl)-L-Ala-D-Glu; MurNAc (beta-methyl)-L-Ala-D-Glu; N alpha-Propionyl -L-Ala-2-amino-6-phosphonohexanoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]