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The map label for this gene is gltD [H]

Identifier: 213158938

GI number: 213158938

Start: 3744025

End: 3745446

Strand: Reverse

Name: gltD [H]

Synonym: AB57_3639

Alternate gene names: 213158938

Gene position: 3745446-3744025 (Counterclockwise)

Preceding gene: 213158939

Following gene: 213158937

Centisome position: 92.47

GC content: 43.25

Gene sequence:

>1422_bases
ATGGCAGAACGCCTAAATAATGACTTTCAATTTCTGGATGTACCACGTCAAGATCCAGAGAAAAAAGATATTACTGTCCG
CAAAGCAGAGTTTGTGGAAATTTATAAACCTTTTACTTCGGAAACTGTAACCAATCAGACGCACCGTTGTTTAGGTTGTG
GTAACCCATATTGCGAATGGAAATGTCCTGTACATAACTACATTCCAAACTGGCTAAAACTCATTGCTGAAGGCCAAATT
TTCCAGGCTGCTGAGCTTTGCCATCAAACAAATACTTTACCTGAGGTATGTGGTCGTGTATGTCCGCAGGACCGTTTGTG
TGAAGGTGCTTGTACCTTAAATGATGGCTTTGGTGCAGTAACTATTGGTAATGCTGAAAAATATATCAATGACACGGCTT
TTGCTTTGGGTTGGCGCCCAGATATGTCACATGTGAAGTGGACAGGTAAAAAAGTTGCAATCATTGGTGCAGGTCCAGCA
GGCCTAGGCTGTGCCGACATTTTAGCTCGTGGTGGTGTTAAACCGGTGGTATTTGACAAGCGTCCTGAGATTGGTGGCTT
ACTAACATTTGGTATTCCAGAATTCAAGATGGAAAAAGATGTGATGAAGCGCCGCCGTGAAATTTTCACTGGGATGGGCA
TTGAATTCCGCTTAAATACTGAAATTGGTAAAGATGTCACGATTGATGAATTACTTGCTGAATATGATGCAGTATTCATG
GGGATGGGAACTTATACCTACATGAAAGGTGGTTTCCCTGGTGAAGATCTTGACGGTGTTTATGATGCGCTTGATTTCTT
AATCTCTAACGTGAACCGTTGTCAGGGATGGGAAAAGGACCCAAGTGAATACATTAGCATGGATGGTAAGAAAGTTATTG
TTCTTGGTGGTGGTGATACCGCAATGGACTGTAACCGTACTTCATTACGTCAAGGTGCCCACGATGTAACATGTGCATAC
CGCCGTGATGAAAGTAATATGCCGGGTTCTGCACGTGAAGTAAAAAATGCGTATGAAGAAGGTGTAAAGTTCTTATTTAA
CCGTCAACCAATCGAGATTGTTGGCGAAAATGGTAAGGTAACAGGCGTAAAAGTGGTAACGACACAAATGGGTGAACCAG
ATAGTCGTGGCCGCCGTAGCCCTGAGCCAATTCCGGGTTCTGAAGAAGTTTTACCTGCTGATGCAGTTTTGCTAGCGTTC
GGTTTCCGTCCAAGCCCTGCTGACTGGTTTGGTAGTGTGAATATTAATCTTGATGGTTCTGGTCGTGTTGTTGCACCTGA
ACAGCAGGAATTTAAATTCCAGACTTCAAACCCAAAAATCTTTGCTGGTGGTGATATGGTACGTGGTTCAGACCTTGTAG
TTACAGCAATCTGGGAAGGTCGCCAAGCTGCTGAAGGTATTTTAGATTACCTTGGTGTTTAA

Upstream 100 bases:

>100_bases
TTTGAAAACTACACAAGCTGACCCACAATAATTCCACGACTGCAAAGACATAGGCAGGTGCGGGCAACAAGGAAATCCGC
GCCTACCCACGGAGAGAGAC

Downstream 100 bases:

>100_bases
TCTAAAAAAATCATTATTAAAGTTTTTGATAAAGCCTGCGATATGCAGGCTTTATTTTTTTACCATTAGTCAGTTTGATG
CTTTTGGCAAATACAATAAG

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MAERLNNDFQFLDVPRQDPEKKDITVRKAEFVEIYKPFTSETVTNQTHRCLGCGNPYCEWKCPVHNYIPNWLKLIAEGQI
FQAAELCHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGNAEKYINDTAFALGWRPDMSHVKWTGKKVAIIGAGPA
GLGCADILARGGVKPVVFDKRPEIGGLLTFGIPEFKMEKDVMKRRREIFTGMGIEFRLNTEIGKDVTIDELLAEYDAVFM
GMGTYTYMKGGFPGEDLDGVYDALDFLISNVNRCQGWEKDPSEYISMDGKKVIVLGGGDTAMDCNRTSLRQGAHDVTCAY
RRDESNMPGSAREVKNAYEEGVKFLFNRQPIEIVGENGKVTGVKVVTTQMGEPDSRGRRSPEPIPGSEEVLPADAVLLAF
GFRPSPADWFGSVNINLDGSGRVVAPEQQEFKFQTSNPKIFAGGDMVRGSDLVVTAIWEGRQAAEGILDYLGV

Sequences:

>Translated_473_residues
MAERLNNDFQFLDVPRQDPEKKDITVRKAEFVEIYKPFTSETVTNQTHRCLGCGNPYCEWKCPVHNYIPNWLKLIAEGQI
FQAAELCHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGNAEKYINDTAFALGWRPDMSHVKWTGKKVAIIGAGPA
GLGCADILARGGVKPVVFDKRPEIGGLLTFGIPEFKMEKDVMKRRREIFTGMGIEFRLNTEIGKDVTIDELLAEYDAVFM
GMGTYTYMKGGFPGEDLDGVYDALDFLISNVNRCQGWEKDPSEYISMDGKKVIVLGGGDTAMDCNRTSLRQGAHDVTCAY
RRDESNMPGSAREVKNAYEEGVKFLFNRQPIEIVGENGKVTGVKVVTTQMGEPDSRGRRSPEPIPGSEEVLPADAVLLAF
GFRPSPADWFGSVNINLDGSGRVVAPEQQEFKFQTSNPKIFAGGDMVRGSDLVVTAIWEGRQAAEGILDYLGV
>Mature_472_residues
AERLNNDFQFLDVPRQDPEKKDITVRKAEFVEIYKPFTSETVTNQTHRCLGCGNPYCEWKCPVHNYIPNWLKLIAEGQIF
QAAELCHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGNAEKYINDTAFALGWRPDMSHVKWTGKKVAIIGAGPAG
LGCADILARGGVKPVVFDKRPEIGGLLTFGIPEFKMEKDVMKRRREIFTGMGIEFRLNTEIGKDVTIDELLAEYDAVFMG
MGTYTYMKGGFPGEDLDGVYDALDFLISNVNRCQGWEKDPSEYISMDGKKVIVLGGGDTAMDCNRTSLRQGAHDVTCAYR
RDESNMPGSAREVKNAYEEGVKFLFNRQPIEIVGENGKVTGVKVVTTQMGEPDSRGRRSPEPIPGSEEVLPADAVLLAFG
FRPSPADWFGSVNINLDGSGRVVAPEQQEFKFQTSNPKIFAGGDMVRGSDLVVTAIWEGRQAAEGILDYLGV

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=452, Percent_Identity=29.4247787610619, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI111118983, Length=166, Percent_Identity=30.1204819277108, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI111118981, Length=175, Percent_Identity=30.2857142857143, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1789606, Length=470, Percent_Identity=69.1489361702128, Blast_Score=683, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=461, Percent_Identity=59.6529284164859, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI87082180, Length=456, Percent_Identity=54.6052631578947, Blast_Score=503, Evalue=1e-143,
Organism=Escherichia coli, GI1788468, Length=442, Percent_Identity=29.4117647058824, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789243, Length=421, Percent_Identity=29.4536817102138, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17570289, Length=494, Percent_Identity=34.6153846153846, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI71984108, Length=466, Percent_Identity=29.3991416309013, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17543860, Length=174, Percent_Identity=29.8850574712644, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320030, Length=491, Percent_Identity=34.4195519348269, Blast_Score=236, Evalue=8e-63,
Organism=Drosophila melanogaster, GI28574881, Length=482, Percent_Identity=37.9668049792531, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24665539, Length=482, Percent_Identity=37.9668049792531, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24665547, Length=482, Percent_Identity=37.9668049792531, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24665543, Length=482, Percent_Identity=37.9668049792531, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24640763, Length=462, Percent_Identity=28.1385281385281, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI18858217, Length=462, Percent_Identity=28.1385281385281, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52173; Mature: 52042

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERLNNDFQFLDVPRQDPEKKDITVRKAEFVEIYKPFTSETVTNQTHRCLGCGNPYCEW
CCCCCCCCCEEEECCCCCCCCCCEEEEHHHHHHHHCCCCCCHHCCCCHHEEECCCCCCEE
KCPVHNYIPNWLKLIAEGQIFQAAELCHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAV
ECCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCEEECCCCCEE
TIGNAEKYINDTAFALGWRPDMSHVKWTGKKVAIIGAGPAGLGCADILARGGVKPVVFDK
EECCHHHHHCCCEEEEECCCCCCCEEECCCEEEEEECCCCCCCHHHHHHCCCCCEEEECC
RPEIGGLLTFGIPEFKMEKDVMKRRREIFTGMGIEFRLNTEIGKDVTIDELLAEYDAVFM
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHEE
GMGTYTYMKGGFPGEDLDGVYDALDFLISNVNRCQGWEKDPSEYISMDGKKVIVLGGGDT
CCCHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEECCCCC
AMDCNRTSLRQGAHDVTCAYRRDESNMPGSAREVKNAYEEGVKFLFNRQPIEIVGENGKV
CCCCCHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCE
TGVKVVTTQMGEPDSRGRRSPEPIPGSEEVLPADAVLLAFGFRPSPADWFGSVNINLDGS
EEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEEECCC
GRVVAPEQQEFKFQTSNPKIFAGGDMVRGSDLVVTAIWEGRQAAEGILDYLGV
CCEECCCHHHCEEECCCCEEEECCCEECCCCEEEEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure 
AERLNNDFQFLDVPRQDPEKKDITVRKAEFVEIYKPFTSETVTNQTHRCLGCGNPYCEW
CCCCCCCCEEEECCCCCCCCCCEEEEHHHHHHHHCCCCCCHHCCCCHHEEECCCCCCEE
KCPVHNYIPNWLKLIAEGQIFQAAELCHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAV
ECCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCEEECCCCCEE
TIGNAEKYINDTAFALGWRPDMSHVKWTGKKVAIIGAGPAGLGCADILARGGVKPVVFDK
EECCHHHHHCCCEEEEECCCCCCCEEECCCEEEEEECCCCCCCHHHHHHCCCCCEEEECC
RPEIGGLLTFGIPEFKMEKDVMKRRREIFTGMGIEFRLNTEIGKDVTIDELLAEYDAVFM
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHEE
GMGTYTYMKGGFPGEDLDGVYDALDFLISNVNRCQGWEKDPSEYISMDGKKVIVLGGGDT
CCCHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEECCCCC
AMDCNRTSLRQGAHDVTCAYRRDESNMPGSAREVKNAYEEGVKFLFNRQPIEIVGENGKV
CCCCCHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCE
TGVKVVTTQMGEPDSRGRRSPEPIPGSEEVLPADAVLLAFGFRPSPADWFGSVNINLDGS
EEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEEECCC
GRVVAPEQQEFKFQTSNPKIFAGGDMVRGSDLVVTAIWEGRQAAEGILDYLGV
CCEECCCHHHCEEECCCCEEEECCCEECCCCEEEEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]