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The map label for this gene is cmr [H]

Identifier: 213158900

GI number: 213158900

Start: 3708508

End: 3709737

Strand: Reverse

Name: cmr [H]

Synonym: AB57_3600

Alternate gene names: 213158900

Gene position: 3709737-3708508 (Counterclockwise)

Preceding gene: 213158901

Following gene: 213158895

Centisome position: 91.59

GC content: 38.46

Gene sequence:

>1230_bases
ATGAAAAATATTCAAACGACAGCGTTGAACCGTACAACGTTGATGTTTCCTTTAGCTTTAGTGCTATTTGAATTTGCGGT
TTATATTGGTAATGACCTGATTCAGCCAGCCATGTTGGCAATTACTGAAGATTTCGGTGTAAGTGCAACTTGGGCGCCGT
CTTCAATGTCATTTTATTTATTAGGTGGTGCATCTGTTGCATGGTTACTAGGACCTTTATCAGATCGATTAGGCCGTAAA
AAAGTACTTTTGTCTGGTGTTTTATTTTTTGCCTTGTGCTGTTTTTTAATTTTATTAACGCGACAAATTGAACACTTTCT
GACATTACGTTTTTTACAAGGTATTGGGTTAAGCGTAATTTCAGCAGTGGGATATGCCGCAATTCAGGAAAACTTTGCCG
AGCGTGATGCGATTAAAGTCATGGCCCTCATGGCAAATATTTCATTGCTTGCGCCTTTGCTAGGGCCTGTACTTGGAGCT
TTCTTAATTGATTATGTTTCATGGCACTGGGGCTTTGTGGCAATTGCTTTACTTGCACTGCTGAGCTGGGTTGGCTTAAA
GAAACAAATGCCTAGTCATAAGGTGAGTGTAACCAAACAGCCTTTTAGTTATCTTTTTGATGACTTTAAAAAGGTATTTA
GCAATCGACAGTTCCTTGGGTTAACGTTGGCCTTACCACTTGTGGGTATGCCGCTTATGCTTTGGATTGCATTATCGCCA
ATTATTTTGGTCGATGAACTGAAGCTAACGAGTGTGCAATATGGTTTAGCACAGTTCCCGGTATTTTTAGGTTTAATTGT
TGGTAACATTGTTTTAATTAAAATTATTGACCGTTTAGCTTTAGGTAAAACTGTCTTAATTGGCTTACCGATTATGCTCA
CTGGTACGCTAATTTTAATATTAGGTGTTGTCTGGCAAGCCTATTTAATTCCTTGCTTATTAATAGGTATGACACTCATT
TGTTTTGGTGAAGGAATTAGCTTCTCAGTGTTGTACCGTTTTGCGCTCATGTCATCTGAAGTGTCAAAAGGAACTGTAGC
TGCTGCTGTTTCAATGCTATTGATGACGAGCTTCTTTGCAATGATTGAGCTTGTACGCTATCTCTACACTCAATTTCATT
TATGGGCTTTTGTCTTATCTGCTTTTGCATTTATTGCCTTGTGGTTTACTCAACCACGTTTAGCTTTAAAACGAGAAATG
CAGGAAAGAGTTGCACAAGACTTACATTAA

Upstream 100 bases:

>100_bases
AAAGATTGACTGACCATACTGTCATCGTCATGACTACATTATTTATTTCAATATGTAGTTGCTGAGACATAGTGGCCGCT
ATTTTGAAGTGTACAAACTC

Downstream 100 bases:

>100_bases
AAAAATACCGGAATTTATATTCCGGTATTTTTTTATTCTATTTAATTTCATGCTCTAAAAGTTTGCCTTTTAAGCTTATG
AGTTCAGGCAAGGTTTCTAA

Product: chloramphenicol resistance pump cmr

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Chloramphenicol resistance pump cmr [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MKNIQTTALNRTTLMFPLALVLFEFAVYIGNDLIQPAMLAITEDFGVSATWAPSSMSFYLLGGASVAWLLGPLSDRLGRK
KVLLSGVLFFALCCFLILLTRQIEHFLTLRFLQGIGLSVISAVGYAAIQENFAERDAIKVMALMANISLLAPLLGPVLGA
FLIDYVSWHWGFVAIALLALLSWVGLKKQMPSHKVSVTKQPFSYLFDDFKKVFSNRQFLGLTLALPLVGMPLMLWIALSP
IILVDELKLTSVQYGLAQFPVFLGLIVGNIVLIKIIDRLALGKTVLIGLPIMLTGTLILILGVVWQAYLIPCLLIGMTLI
CFGEGISFSVLYRFALMSSEVSKGTVAAAVSMLLMTSFFAMIELVRYLYTQFHLWAFVLSAFAFIALWFTQPRLALKREM
QERVAQDLH

Sequences:

>Translated_409_residues
MKNIQTTALNRTTLMFPLALVLFEFAVYIGNDLIQPAMLAITEDFGVSATWAPSSMSFYLLGGASVAWLLGPLSDRLGRK
KVLLSGVLFFALCCFLILLTRQIEHFLTLRFLQGIGLSVISAVGYAAIQENFAERDAIKVMALMANISLLAPLLGPVLGA
FLIDYVSWHWGFVAIALLALLSWVGLKKQMPSHKVSVTKQPFSYLFDDFKKVFSNRQFLGLTLALPLVGMPLMLWIALSP
IILVDELKLTSVQYGLAQFPVFLGLIVGNIVLIKIIDRLALGKTVLIGLPIMLTGTLILILGVVWQAYLIPCLLIGMTLI
CFGEGISFSVLYRFALMSSEVSKGTVAAAVSMLLMTSFFAMIELVRYLYTQFHLWAFVLSAFAFIALWFTQPRLALKREM
QERVAQDLH
>Mature_409_residues
MKNIQTTALNRTTLMFPLALVLFEFAVYIGNDLIQPAMLAITEDFGVSATWAPSSMSFYLLGGASVAWLLGPLSDRLGRK
KVLLSGVLFFALCCFLILLTRQIEHFLTLRFLQGIGLSVISAVGYAAIQENFAERDAIKVMALMANISLLAPLLGPVLGA
FLIDYVSWHWGFVAIALLALLSWVGLKKQMPSHKVSVTKQPFSYLFDDFKKVFSNRQFLGLTLALPLVGMPLMLWIALSP
IILVDELKLTSVQYGLAQFPVFLGLIVGNIVLIKIIDRLALGKTVLIGLPIMLTGTLILILGVVWQAYLIPCLLIGMTLI
CFGEGISFSVLYRFALMSSEVSKGTVAAAVSMLLMTSFFAMIELVRYLYTQFHLWAFVLSAFAFIALWFTQPRLALKREM
QERVAQDLH

Specific function: Efflux pump driven by the proton motive force. Confers resistance to basic, aromatic and lipophilic drugs as well as neutral, non-aromatic, zwitterionic or non-aromatic hydrophilic antibiotics [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787065, Length=363, Percent_Identity=44.9035812672176, Blast_Score=297, Evalue=9e-82,
Organism=Escherichia coli, GI1790794, Length=404, Percent_Identity=37.1287128712871, Blast_Score=261, Evalue=8e-71,
Organism=Escherichia coli, GI48994889, Length=247, Percent_Identity=28.3400809716599, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1788509, Length=230, Percent_Identity=24.7826086956522, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790146, Length=315, Percent_Identity=25.3968253968254, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790195, Length=299, Percent_Identity=23.7458193979933, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45347; Mature: 45347

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]