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The map label for this gene is astE

Identifier: 213158884

GI number: 213158884

Start: 3689046

End: 3690020

Strand: Reverse

Name: astE

Synonym: AB57_3583

Alternate gene names: 213158884

Gene position: 3690020-3689046 (Counterclockwise)

Preceding gene: 213158885

Following gene: 213158882

Centisome position: 91.1

GC content: 40.72

Gene sequence:

>975_bases
ATGCAAGATTTTCTAGCATTAACCTTACAAGGTGAGCAACCTGCAACGCGCGAGGGAAAACAAGCAAATTTTTCTTGGCG
TTGGTTAGGTGAGGGACTTCTTGAATGTACACCACATGCGCAGTACGACAAAGCTGTCGTACTGTCAGCTGGAGTACATG
GGAATGAAACGGCACCAATTGAATTGCTATCTCATCTTTGCACCGATTTATTTGCTGGGCGTTTAAAGCTTGCGGTGAGA
TTATTGCTTGTATTGGGTAATCCCTATGCGATGCGTCAAGGCAAGCGTTATGTACATGACGATGTAAACCGTATGTTCTG
TGGTGGGTATAAAAATCTGCCTGTAACTGAAGAATCTAAGCGTGCTGAGGTTTTAGAGCAGACTGTTGCCACTTTCTTTC
AGGAAAGTTCGAGTCAGGCAAAACGTTATCATTATGATTTGCACACCGCAATTCGGGCTTCATTATTACCAACATTTGCT
TTATTTCCATATCAAACGCATGGCTATGATGCTGATTTAACGGCAAGTCTTGAAGCAGCTGATTTGGATGCCTTGGTTTA
TCATAATGCGTTAGGTAAAACATTTACCCATTTTACGAGTGAGAATTTTAAGGCTGCGAGTGCAACTTTAGAGTTGGGCA
AAGCGTTGCCATTTGGTCAAAATGACTTGAGTCAATTTGCTTCAATTGATGAAGTCATTCGTAACGTGGTTTCTGAGCAA
GCTTTACCAGTGCGTAATAAACCAAAAATTCGTGTGTTTCAGGTATCTGACTCTTTAATAAAAAAAGATGAAGAGTTCCA
CATGAATTTATCTGCTGAGGCTCCAAACTTTTCAACTTTTACTAAAGGCGAAATCATTGCGACTCAACCTTCTGGTAACT
ATGTAGTTGAACAAGATCAGGTCTGGATTTTATTTCCAAACCCGAATGTGAAGATTGGTTTACGTGCAGGCTTGGTTTTA
ACTGAAACAATCTGA

Upstream 100 bases:

>100_bases
TCCGCATTTACTCATGGAAAGCCGTATGGCACTTGATGAACTGACTAAAATTTTAGGTTTAGGTTCGGTTTATCCTTTCC
AAAAATAATAAGGAGGTGAC

Downstream 100 bases:

>100_bases
ATAGTTAATTTTAGACAAATAAAAAAGGGCGATTCATCGCCCTTTTTTATTGCTAACTTAATTAGTTAGTACCGAAAAGA
CCTTTAATAAAAGATTTCTT

Product: succinylglutamate desuccinylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MQDFLALTLQGEQPATREGKQANFSWRWLGEGLLECTPHAQYDKAVVLSAGVHGNETAPIELLSHLCTDLFAGRLKLAVR
LLLVLGNPYAMRQGKRYVHDDVNRMFCGGYKNLPVTEESKRAEVLEQTVATFFQESSSQAKRYHYDLHTAIRASLLPTFA
LFPYQTHGYDADLTASLEAADLDALVYHNALGKTFTHFTSENFKAASATLELGKALPFGQNDLSQFASIDEVIRNVVSEQ
ALPVRNKPKIRVFQVSDSLIKKDEEFHMNLSAEAPNFSTFTKGEIIATQPSGNYVVEQDQVWILFPNPNVKIGLRAGLVL
TETI

Sequences:

>Translated_324_residues
MQDFLALTLQGEQPATREGKQANFSWRWLGEGLLECTPHAQYDKAVVLSAGVHGNETAPIELLSHLCTDLFAGRLKLAVR
LLLVLGNPYAMRQGKRYVHDDVNRMFCGGYKNLPVTEESKRAEVLEQTVATFFQESSSQAKRYHYDLHTAIRASLLPTFA
LFPYQTHGYDADLTASLEAADLDALVYHNALGKTFTHFTSENFKAASATLELGKALPFGQNDLSQFASIDEVIRNVVSEQ
ALPVRNKPKIRVFQVSDSLIKKDEEFHMNLSAEAPNFSTFTKGEIIATQPSGNYVVEQDQVWILFPNPNVKIGLRAGLVL
TETI
>Mature_324_residues
MQDFLALTLQGEQPATREGKQANFSWRWLGEGLLECTPHAQYDKAVVLSAGVHGNETAPIELLSHLCTDLFAGRLKLAVR
LLLVLGNPYAMRQGKRYVHDDVNRMFCGGYKNLPVTEESKRAEVLEQTVATFFQESSSQAKRYHYDLHTAIRASLLPTFA
LFPYQTHGYDADLTASLEAADLDALVYHNALGKTFTHFTSENFKAASATLELGKALPFGQNDLSQFASIDEVIRNVVSEQ
ALPVRNKPKIRVFQVSDSLIKKDEEFHMNLSAEAPNFSTFTKGEIIATQPSGNYVVEQDQVWILFPNPNVKIGLRAGLVL
TETI

Specific function: Transforms N(2)-succinylglutamate into succinate and glutamate

COG id: COG2988

COG function: function code E; Succinylglutamate desuccinylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily

Homologues:

Organism=Escherichia coli, GI1788040, Length=320, Percent_Identity=44.0625, Blast_Score=279, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASTE_ACIB3 (B7GVV1)

Other databases:

- EMBL:   CP001172
- RefSeq:   YP_002324314.1
- ProteinModelPortal:   B7GVV1
- SMR:   B7GVV1
- GeneID:   7059720
- GenomeReviews:   CP001172_GR
- KEGG:   abb:ABBFA_000381
- HOGENOM:   HBG298271
- OMA:   YLHNDLN
- ProtClustDB:   PRK05324
- HAMAP:   MF_00767
- InterPro:   IPR007036
- InterPro:   IPR016681
- TIGRFAMs:   TIGR03242

Pfam domain/function: PF04952 AstE_AspA

EC number: =3.5.1.96

Molecular weight: Translated: 36092; Mature: 36092

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: ACT_SITE 211-211

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDFLALTLQGEQPATREGKQANFSWRWLGEGLLECTPHAQYDKAVVLSAGVHGNETAPI
CCCEEEEEEECCCCCCCCCCCCCCEEEECCCCHHHCCCCCCCCCEEEEECCCCCCCCCHH
ELLSHLCTDLFAGRLKLAVRLLLVLGNPYAMRQGKRYVHDDVNRMFCGGYKNLPVTEESK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHH
RAEVLEQTVATFFQESSSQAKRYHYDLHTAIRASLLPTFALFPYQTHGYDADLTASLEAA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCHH
DLDALVYHNALGKTFTHFTSENFKAASATLELGKALPFGQNDLSQFASIDEVIRNVVSEQ
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
ALPVRNKPKIRVFQVSDSLIKKDEEFHMNLSAEAPNFSTFTKGEIIATQPSGNYVVEQDQ
CCCCCCCCCEEEEEECHHHHCCCCHHEEEEECCCCCCCCCCCCCEEEECCCCCEEEECCC
VWILFPNPNVKIGLRAGLVLTETI
EEEEECCCCEEEEEEECEEEEECC
>Mature Secondary Structure
MQDFLALTLQGEQPATREGKQANFSWRWLGEGLLECTPHAQYDKAVVLSAGVHGNETAPI
CCCEEEEEEECCCCCCCCCCCCCCEEEECCCCHHHCCCCCCCCCEEEEECCCCCCCCCHH
ELLSHLCTDLFAGRLKLAVRLLLVLGNPYAMRQGKRYVHDDVNRMFCGGYKNLPVTEESK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHH
RAEVLEQTVATFFQESSSQAKRYHYDLHTAIRASLLPTFALFPYQTHGYDADLTASLEAA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCHH
DLDALVYHNALGKTFTHFTSENFKAASATLELGKALPFGQNDLSQFASIDEVIRNVVSEQ
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
ALPVRNKPKIRVFQVSDSLIKKDEEFHMNLSAEAPNFSTFTKGEIIATQPSGNYVVEQDQ
CCCCCCCCCEEEEEECHHHHCCCCHHEEEEECCCCCCCCCCCCCEEEECCCCCEEEECCC
VWILFPNPNVKIGLRAGLVLTETI
EEEEECCCCEEEEEEECEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA