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The map label for this gene is glyS [H]

Identifier: 213158873

GI number: 213158873

Start: 3678649

End: 3680718

Strand: Direct

Name: glyS [H]

Synonym: AB57_3572

Alternate gene names: 213158873

Gene position: 3678649-3680718 (Clockwise)

Preceding gene: 213158872

Following gene: 213158874

Centisome position: 90.82

GC content: 42.03

Gene sequence:

>2070_bases
ATGAGCAAACATACTGTTTTATTCGAACTTGGCTGTGAAGAACTTCCACCAAAAAGCTTAAAAACATTACGTGATGCACT
ACAAGCAGAAACGGTAAAAGGCTTAAATGAAGCTGGTTTAAACTTCGCATCTGTTGAAGCTTATGCCGCTCCACGTCGTT
TAGCGCTTAAAATTGTAGATGTAGATGCTGCGCAAGCGGATACACAAAAACGTTTTGACGGTCCTGCTGTTCAAGCAGCT
TACGATGCAGAAGGCAAACCGACTAAAGCACTTGAAGGCTTTATGCGCGGTCAAGGCATTACTGTTGAGCAGCTTTCTAC
GTTCCAAGCAGGTAAAGTTGAAAAAGTTTGCTATTTAAAAGATGTGAAGGGTCAAAGCCTTGACACTTTGCTTCCACAGA
TTTTACAAACTGCATTAGACAATTTACCAATTGCTAAGCGCATGCGTTCTGCTGCAAGCCGTACCGAATTTGTACGTCCA
GTGAAATGGGTTGTCTTACTCAAAGATGACCAAGTTATTGAGGCGACTATTCAAGATCACAAAGCTGGTAATGTGACTTA
TGGTCACCGTTTCCATGCACCTGAAGCTGTGACCTTAGCGCATGCAAATGACTATTTAGCTGCGTTAGAAAAAGCATATG
TTGTAGCGAACTTTGAAAAGCGTCAGGCAACCATTCAAGAACAAGTGAAAAAGTTAGCTGATGAAGTGAATGCAACTGCG
ATTGTTCCAGCTGACTTACTTGACGAAGTAACAAGTCTGGTTGAATGGCCTGTGGCTTTACGTGCAACTTTTGAAGAACG
CTACTTAGCTGTTCCTCAAGAAGCACTCATCACGACAATGCAAGACAACCAGAAGTATTTCTGTTTGATCAACGCGGAAG
GTAAATTACAGCCTTACTTTATTACTGTTTCAAATATTGAGTCTAAAGATCCGACTCAAATTATTGAAGGTAACGAAAAA
GTTGTTCGCCCTCGTTTGTCTGATGCTGAGTTCTTCTTCTTACAAGATCAAAAGCAACCGCTTGCATCTCGTAAAGAAAA
ACTAGCAAACATGGTATTCCAAGCACAACTTGGTACGCTTTGGGACAAATCAACACGGATTGCAAAACTTGCAGTTGCAT
TATCTTCAATCACTGGTGCAAACCCAGCTGATGCTGAAAAAGCTGCATTACTTGCTAAATGCGACTTAACCTCTGAGCTT
GTTGGTGAATTCCCAGAACTTCAAGGTATTGCGGGTACTTACTATGCGCGTATTGAAGGCGAAAACACAGAAGTTTCAGA
AGCTTTAGGTGAGCAATATTTACCAAAATTTGCGGGTGATGTTTTACCTAAAACCAAAACAGGTACAACCATTGCCCTTT
CTGACCGTTTAGATACGCTTGTTGGTATTTTCGGTATTGGACAAGCGCCTACAGGTTCTAAAGACCCGTTTGCTCTTCGC
CGTTCAGCAATTGGTATCTTACGTTTAATCATTGAAAATGAATTAGATGTAACGATTGAAGAATTAGTGAATCTTGCACT
TCAAGGTTATGGCGATATCGTTAAAGACCACGATAAAACTCGTGCTGATGCTGTTGCATTCTTGGAAGGTCGTTACCGTG
CTAAGTACGAAGACCAAGGTGTTGCTGTTGACGTACTTCAAGCCGTTCAAGCTCTTGCACCAAAATCTCCACTTGATTTT
GACAAGCGTGTAAATGCTGTTAACCACTTCCGTACACTACCTGAAGCTGCTGCACTTGCTGCTGCAAATAAACGTGTTGC
GAACATTCTTGCAAAAGAAGCAACACCTGAAGGTTCTGTAGTTGAAGCAAACTTGGTTGAAGACGCTGAAAAAGCATTAT
TTGCTGAACTTCAAGCAGTGACACCTGTGGTTGAACCATTACTTGCAGCAAAAGATTACACAGCTGCCCTTTCTAAGCTT
GCAGCACTTCGCGCGCCGATTGATGCATTCTTTGATGGCGTAATGGTAATGGCAGATGATGCTGGCCTGAAAGCAAATCG
TTTACGTTTATTGGCTCAGTTGCGTAACTTGTTTACTGCTGTGGCTGATGTGAGTGTATTGCAAGGTTAA

Upstream 100 bases:

>100_bases
AGCTTGGCTTCCCAATGGCAGAACCACACTTACGTGATGAAGTATTGGCACAGCTAAAAGCACAAGCTGAAAGTGAAGCA
GCAAAGGCGGAGAAAAACTA

Downstream 100 bases:

>100_bases
TAAAAAGGATAGAGGAGGTGAAAACACCTCCTTTTTTTAATATATGGATGCAAAATATTATTTTGAAACAATAAAAAATG
ATCTTGCGATTGGCAAAGAT

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 689; Mature: 688

Protein sequence:

>689_residues
MSKHTVLFELGCEELPPKSLKTLRDALQAETVKGLNEAGLNFASVEAYAAPRRLALKIVDVDAAQADTQKRFDGPAVQAA
YDAEGKPTKALEGFMRGQGITVEQLSTFQAGKVEKVCYLKDVKGQSLDTLLPQILQTALDNLPIAKRMRSAASRTEFVRP
VKWVVLLKDDQVIEATIQDHKAGNVTYGHRFHAPEAVTLAHANDYLAALEKAYVVANFEKRQATIQEQVKKLADEVNATA
IVPADLLDEVTSLVEWPVALRATFEERYLAVPQEALITTMQDNQKYFCLINAEGKLQPYFITVSNIESKDPTQIIEGNEK
VVRPRLSDAEFFFLQDQKQPLASRKEKLANMVFQAQLGTLWDKSTRIAKLAVALSSITGANPADAEKAALLAKCDLTSEL
VGEFPELQGIAGTYYARIEGENTEVSEALGEQYLPKFAGDVLPKTKTGTTIALSDRLDTLVGIFGIGQAPTGSKDPFALR
RSAIGILRLIIENELDVTIEELVNLALQGYGDIVKDHDKTRADAVAFLEGRYRAKYEDQGVAVDVLQAVQALAPKSPLDF
DKRVNAVNHFRTLPEAAALAAANKRVANILAKEATPEGSVVEANLVEDAEKALFAELQAVTPVVEPLLAAKDYTAALSKL
AALRAPIDAFFDGVMVMADDAGLKANRLRLLAQLRNLFTAVADVSVLQG

Sequences:

>Translated_689_residues
MSKHTVLFELGCEELPPKSLKTLRDALQAETVKGLNEAGLNFASVEAYAAPRRLALKIVDVDAAQADTQKRFDGPAVQAA
YDAEGKPTKALEGFMRGQGITVEQLSTFQAGKVEKVCYLKDVKGQSLDTLLPQILQTALDNLPIAKRMRSAASRTEFVRP
VKWVVLLKDDQVIEATIQDHKAGNVTYGHRFHAPEAVTLAHANDYLAALEKAYVVANFEKRQATIQEQVKKLADEVNATA
IVPADLLDEVTSLVEWPVALRATFEERYLAVPQEALITTMQDNQKYFCLINAEGKLQPYFITVSNIESKDPTQIIEGNEK
VVRPRLSDAEFFFLQDQKQPLASRKEKLANMVFQAQLGTLWDKSTRIAKLAVALSSITGANPADAEKAALLAKCDLTSEL
VGEFPELQGIAGTYYARIEGENTEVSEALGEQYLPKFAGDVLPKTKTGTTIALSDRLDTLVGIFGIGQAPTGSKDPFALR
RSAIGILRLIIENELDVTIEELVNLALQGYGDIVKDHDKTRADAVAFLEGRYRAKYEDQGVAVDVLQAVQALAPKSPLDF
DKRVNAVNHFRTLPEAAALAAANKRVANILAKEATPEGSVVEANLVEDAEKALFAELQAVTPVVEPLLAAKDYTAALSKL
AALRAPIDAFFDGVMVMADDAGLKANRLRLLAQLRNLFTAVADVSVLQG
>Mature_688_residues
SKHTVLFELGCEELPPKSLKTLRDALQAETVKGLNEAGLNFASVEAYAAPRRLALKIVDVDAAQADTQKRFDGPAVQAAY
DAEGKPTKALEGFMRGQGITVEQLSTFQAGKVEKVCYLKDVKGQSLDTLLPQILQTALDNLPIAKRMRSAASRTEFVRPV
KWVVLLKDDQVIEATIQDHKAGNVTYGHRFHAPEAVTLAHANDYLAALEKAYVVANFEKRQATIQEQVKKLADEVNATAI
VPADLLDEVTSLVEWPVALRATFEERYLAVPQEALITTMQDNQKYFCLINAEGKLQPYFITVSNIESKDPTQIIEGNEKV
VRPRLSDAEFFFLQDQKQPLASRKEKLANMVFQAQLGTLWDKSTRIAKLAVALSSITGANPADAEKAALLAKCDLTSELV
GEFPELQGIAGTYYARIEGENTEVSEALGEQYLPKFAGDVLPKTKTGTTIALSDRLDTLVGIFGIGQAPTGSKDPFALRR
SAIGILRLIIENELDVTIEELVNLALQGYGDIVKDHDKTRADAVAFLEGRYRAKYEDQGVAVDVLQAVQALAPKSPLDFD
KRVNAVNHFRTLPEAAALAAANKRVANILAKEATPEGSVVEANLVEDAEKALFAELQAVTPVVEPLLAAKDYTAALSKLA
ALRAPIDAFFDGVMVMADDAGLKANRLRLLAQLRNLFTAVADVSVLQG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=690, Percent_Identity=48.695652173913, Blast_Score=613, Evalue=1e-176,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 75053; Mature: 74921

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKHTVLFELGCEELPPKSLKTLRDALQAETVKGLNEAGLNFASVEAYAAPRRLALKIVD
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCHHHHCCCEEEHHHHHCCCEEEEEEEE
VDAAQADTQKRFDGPAVQAAYDAEGKPTKALEGFMRGQGITVEQLSTFQAGKVEKVCYLK
CCCHHCHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCEEEEE
DVKGQSLDTLLPQILQTALDNLPIAKRMRSAASRTEFVRPVKWVVLLKDDQVIEATIQDH
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEHHHHHC
KAGNVTYGHRFHAPEAVTLAHANDYLAALEKAYVVANFEKRQATIQEQVKKLADEVNATA
CCCCCCCCCEECCCCEEEEECCHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHCCCEE
IVPADLLDEVTSLVEWPVALRATFEERYLAVPQEALITTMQDNQKYFCLINAEGKLQPYF
EECHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHEECCCEEEEEEECCCCCEEEE
ITVSNIESKDPTQIIEGNEKVVRPRLSDAEFFFLQDQKQPLASRKEKLANMVFQAQLGTL
EEEECCCCCCCHHHHCCCCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
WDKSTRIAKLAVALSSITGANPADAEKAALLAKCDLTSELVGEFPELQGIAGTYYARIEG
HCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHCCCHHHCCCCCEEEEEEEC
ENTEVSEALGEQYLPKFAGDVLPKTKTGTTIALSDRLDTLVGIFGIGQAPTGSKDPFALR
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCHHHH
RSAIGILRLIIENELDVTIEELVNLALQGYGDIVKDHDKTRADAVAFLEGRYRAKYEDQG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCC
VAVDVLQAVQALAPKSPLDFDKRVNAVNHFRTLPEAAALAAANKRVANILAKEATPEGSV
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCE
VEANLVEDAEKALFAELQAVTPVVEPLLAAKDYTAALSKLAALRAPIDAFFDGVMVMADD
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCEEEEECC
AGLKANRLRLLAQLRNLFTAVADVSVLQG
CCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKHTVLFELGCEELPPKSLKTLRDALQAETVKGLNEAGLNFASVEAYAAPRRLALKIVD
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCHHHHCCCEEEHHHHHCCCEEEEEEEE
VDAAQADTQKRFDGPAVQAAYDAEGKPTKALEGFMRGQGITVEQLSTFQAGKVEKVCYLK
CCCHHCHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCEEEEE
DVKGQSLDTLLPQILQTALDNLPIAKRMRSAASRTEFVRPVKWVVLLKDDQVIEATIQDH
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEHHHHHC
KAGNVTYGHRFHAPEAVTLAHANDYLAALEKAYVVANFEKRQATIQEQVKKLADEVNATA
CCCCCCCCCEECCCCEEEEECCHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHCCCEE
IVPADLLDEVTSLVEWPVALRATFEERYLAVPQEALITTMQDNQKYFCLINAEGKLQPYF
EECHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHEECCCEEEEEEECCCCCEEEE
ITVSNIESKDPTQIIEGNEKVVRPRLSDAEFFFLQDQKQPLASRKEKLANMVFQAQLGTL
EEEECCCCCCCHHHHCCCCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
WDKSTRIAKLAVALSSITGANPADAEKAALLAKCDLTSELVGEFPELQGIAGTYYARIEG
HCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHCCCHHHCCCCCEEEEEEEC
ENTEVSEALGEQYLPKFAGDVLPKTKTGTTIALSDRLDTLVGIFGIGQAPTGSKDPFALR
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCHHHH
RSAIGILRLIIENELDVTIEELVNLALQGYGDIVKDHDKTRADAVAFLEGRYRAKYEDQG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCC
VAVDVLQAVQALAPKSPLDFDKRVNAVNHFRTLPEAAALAAANKRVANILAKEATPEGSV
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCE
VEANLVEDAEKALFAELQAVTPVVEPLLAAKDYTAALSKLAALRAPIDAFFDGVMVMADD
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCEEEEECC
AGLKANRLRLLAQLRNLFTAVADVSVLQG
CCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA