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The map label for this gene is purC [H]

Identifier: 213158744

GI number: 213158744

Start: 4003720

End: 4004439

Strand: Direct

Name: purC [H]

Synonym: AB57_3878

Alternate gene names: 213158744

Gene position: 4003720-4004439 (Clockwise)

Preceding gene: 213158743

Following gene: 213158745

Centisome position: 98.84

GC content: 39.03

Gene sequence:

>720_bases
ATGTTAAAACAAACCTTGCTCTATACTGGTAAAGCAAAATCTGTATATGAAACAGACAATGCCGACCATCTGATTTTAGT
CTTTCGTGATGATGCCTCTGCGTTCAATGGCGAAAAAATCGAACAACTAGATCGTAAAGGCAAGGTGAACAACCGTTTTA
ACGCCTTCATCATGGAAAAACTTGCTGAAGCGGGTATCGAAACGCATTTTGAAAAATTGCTTTCTCCAACTGAAGTTCTT
GTTAAAAAACTTCAAATGATTCCTGTTGAATGTGTAATCCGTAACTATGCAGCAGGTTCTCTATGCCGTCGTTTAGGTGT
AGAAGAAGGTAAAGAGTTAACTCCTCCTACATTTGAATTGTTCTACAAAGATGATGGCCTAGGCGATCCAATGGTTAACG
AATCTCAAGCGATTGCTTTAGGTTGGGCGACTGCTGAACAGTTAGAACAAATGAAAGTGTTAACTTATAAAGTAAATGAT
GTATTAAAAGCATTATTTGCTGAAGGCAACATGATTTTGGTGGACTTCAAACTTGAATTTGGTGTTTTCCATGATCGTAT
CGTACTTGGTGATGAATTCTCTCCAGACGGTTGCCGTTTATGGGACAAAGACACTAAGAAAAAACTCGACAAAGACCGTT
TCCGTCAAGGCTTAGGCGGTGTTGTTGAAGCTTATGAAGAAGTTGCTGCTCGTTTAGGCGTAGATTTATCTGACATCTAA

Upstream 100 bases:

>100_bases
TTTTGCCAGCCCTGATGTTGCCGCAGAAGTGTTAAACCAGATTTACCAAAATTGGCCAGCCTAGACGTTTACTAGGCATT
TTTTTTAAAAAGGTTCCCTC

Downstream 100 bases:

>100_bases
TCAGCTCAACTTTATAAAAACCGAGCATTTCAGCTCGGTTTTTTTATTTTCAGTACTCTAGAATTTAGGAAAAGTAGACA
GATTTGTATATTTAAGTAAA

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MLKQTLLYTGKAKSVYETDNADHLILVFRDDASAFNGEKIEQLDRKGKVNNRFNAFIMEKLAEAGIETHFEKLLSPTEVL
VKKLQMIPVECVIRNYAAGSLCRRLGVEEGKELTPPTFELFYKDDGLGDPMVNESQAIALGWATAEQLEQMKVLTYKVND
VLKALFAEGNMILVDFKLEFGVFHDRIVLGDEFSPDGCRLWDKDTKKKLDKDRFRQGLGGVVEAYEEVAARLGVDLSDI

Sequences:

>Translated_239_residues
MLKQTLLYTGKAKSVYETDNADHLILVFRDDASAFNGEKIEQLDRKGKVNNRFNAFIMEKLAEAGIETHFEKLLSPTEVL
VKKLQMIPVECVIRNYAAGSLCRRLGVEEGKELTPPTFELFYKDDGLGDPMVNESQAIALGWATAEQLEQMKVLTYKVND
VLKALFAEGNMILVDFKLEFGVFHDRIVLGDEFSPDGCRLWDKDTKKKLDKDRFRQGLGGVVEAYEEVAARLGVDLSDI
>Mature_239_residues
MLKQTLLYTGKAKSVYETDNADHLILVFRDDASAFNGEKIEQLDRKGKVNNRFNAFIMEKLAEAGIETHFEKLLSPTEVL
VKKLQMIPVECVIRNYAAGSLCRRLGVEEGKELTPPTFELFYKDDGLGDPMVNESQAIALGWATAEQLEQMKVLTYKVND
VLKALFAEGNMILVDFKLEFGVFHDRIVLGDEFSPDGCRLWDKDTKKKLDKDRFRQGLGGVVEAYEEVAARLGVDLSDI

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family [H]

Homologues:

Organism=Homo sapiens, GI119220557, Length=244, Percent_Identity=34.8360655737705, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI5453539, Length=244, Percent_Identity=34.8360655737705, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI119220559, Length=251, Percent_Identity=33.4661354581673, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI1788820, Length=236, Percent_Identity=66.1016949152542, Blast_Score=320, Evalue=5e-89,
Organism=Caenorhabditis elegans, GI17531275, Length=240, Percent_Identity=32.5, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI18860083, Length=221, Percent_Identity=33.0316742081448, Blast_Score=110, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24583917, Length=211, Percent_Identity=30.8056872037915, Blast_Score=95, Evalue=4e-20,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236 [H]

Pfam domain/function: PF01259 SAICAR_synt [H]

EC number: =6.3.2.6 [H]

Molecular weight: Translated: 26958; Mature: 26958

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS01057 SAICAR_SYNTHETASE_1 ; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKQTLLYTGKAKSVYETDNADHLILVFRDDASAFNGEKIEQLDRKGKVNNRFNAFIMEK
CCCCCEEECCCCCCEEECCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHHHH
LAEAGIETHFEKLLSPTEVLVKKLQMIPVECVIRNYAAGSLCRRLGVEEGKELTPPTFEL
HHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
FYKDDGLGDPMVNESQAIALGWATAEQLEQMKVLTYKVNDVLKALFAEGNMILVDFKLEF
EECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
GVFHDRIVLGDEFSPDGCRLWDKDTKKKLDKDRFRQGLGGVVEAYEEVAARLGVDLSDI
CCEECEEEECCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLKQTLLYTGKAKSVYETDNADHLILVFRDDASAFNGEKIEQLDRKGKVNNRFNAFIMEK
CCCCCEEECCCCCCEEECCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHHHH
LAEAGIETHFEKLLSPTEVLVKKLQMIPVECVIRNYAAGSLCRRLGVEEGKELTPPTFEL
HHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
FYKDDGLGDPMVNESQAIALGWATAEQLEQMKVLTYKVNDVLKALFAEGNMILVDFKLEF
EECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
GVFHDRIVLGDEFSPDGCRLWDKDTKKKLDKDRFRQGLGGVVEAYEEVAARLGVDLSDI
CCEECEEEECCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA