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The map label for this gene is purF [H]

Identifier: 213158653

GI number: 213158653

Start: 2699892

End: 2701433

Strand: Reverse

Name: purF [H]

Synonym: AB57_2607

Alternate gene names: 213158653

Gene position: 2701433-2699892 (Counterclockwise)

Preceding gene: 213158654

Following gene: 213158652

Centisome position: 66.69

GC content: 40.21

Gene sequence:

>1542_bases
ATGTGTGGAGTTGTTGGTATAGCCGGTAAATCGCCAGTGAACCAAATGTTGTTTGATGCATTAACGATGTTGCAACATCG
TGGGCAAGATGCAGCTGGAATAGTAACCTGCCATGAAGGCCGTCTTTTCCTTCGTAAGGATAACGGTATGGTACGTGATG
TTTTTCATACTCGTCATATGAGAGCATTACTTGGTAATTATGGTATCGGTCATGTGCGCTATCCAACTGCCGGTTCTTCG
AGCAGTGCGGAAGCTCAACCATTTTATGTGAACTCACCTTACGGTATTACACTTGCACATAATGGTAACTTGACAAATGC
AGAAGAAATTCATGATGACCTATTCAAAACAGATTTGCGTCACATGAATACGGACTCTGATTCGGAAGTTCTTTTAAACG
TTTTTGCTCATGAGTTGCAAAAGATAGGAACTTTAAATCCTACACCGGAAGATATTTTCCATACCGTTTCGCGTGTACAT
GAGCGCTGCCAAGGTGCTTATGGTGTGGTGGCAATGATTACTGGTCACGGCTTAGTTGGTTTCCGTGATCCAAATGGTAT
CCGTCCGCTTATTTACGGTTCTCGTGTAACTGAGCAAGGTGAGATGGAATATATCATTGCATCTGAATCTGTAGCAATTA
CAGCGCTTGGCTTTAAAATTGAGCGTGATATTGCTCCGGGTGAAGCAATTTTCATTAATGCTGACGGCGAGTTATTTACA
AAGCAATGTGCTGCAGATCCAAAATACCGTCCATGTATTTTTGAATATGTTTATTTTGCTCGTCCAGATGCAATTATTGA
TGGTATTTCGGTTTATAAAGCTCGCTTAAAAATGGGTGAGAAATTAGCGCATAAAATTTTGCGTGACTGGGGTGAAGACC
ATGATATTGACGTGGTTATCCCAATTCCAGATACAAGCCGTACCTCAGCTTTAGAGTTGGCAAATATTCTTGGCGTGAAG
TTCCGTGAAGGTTTTATGAAAAACCGTTATATCGGTCGTACCTTCATTATGCCAGGTCAACAACAACGTAAAAAATCTGT
ACGTCAGAAATTAAACCCTGTTGAGTTAGAGTTTAAAGGCAAGAACGTTCTATTAGTGGATGACTCAATTGTGCGTGGTA
CAACGTGTAATGAGATCATTCAAATGGCGCGTGACTCAGGTGCGAAAAAAGTATACTTTGCTTCAGCTGCACCGAAAGTG
ATGTATCCGAACGTATATGGTATCGATATGCCTGCTAAAACTGAGCTTATTGCTTCAGAGCGCAGCGTAGAAGAAATTCA
GGAAATTATTGGTGCTGATCGTTTAATTTTCCAAGATTTGGAAGATTTGAAGAACGCTGTACGTACAAGTAAAGTACCTA
CATTAACCGAGTTTGATTGCTCTGTTTTTGATGGTATCTATGTAACTGGTGGAATTGATGCAGACTATCTGAATAATTTG
GAGCAGAAACGTAATGATTCTGCGAAAAAGAAAAAAGATGGTTATATTGATGTCAATATTGATGCAGCATCAGTAGATTT
GACCGGTATTAAAGAAGAATAG

Upstream 100 bases:

>100_bases
TCTCCAAAACCAATGGACGAGTCGGAGGAAACAGAACTTCGACCTGACCATTCAACAAAGAATCCTTTTTATTAATTCCT
AGCTTGTCTGCGAGGTTGCT

Downstream 100 bases:

>100_bases
AAATAGCCGAAAAAAGCGGGTATTTCCCGCTTTTTTCTTTTATGATGAAACTTAACAATGTAATTGGGATTATTTACATT
GAAAGAAGTAAGCCACTATT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MCGVVGIAGKSPVNQMLFDALTMLQHRGQDAAGIVTCHEGRLFLRKDNGMVRDVFHTRHMRALLGNYGIGHVRYPTAGSS
SSAEAQPFYVNSPYGITLAHNGNLTNAEEIHDDLFKTDLRHMNTDSDSEVLLNVFAHELQKIGTLNPTPEDIFHTVSRVH
ERCQGAYGVVAMITGHGLVGFRDPNGIRPLIYGSRVTEQGEMEYIIASESVAITALGFKIERDIAPGEAIFINADGELFT
KQCAADPKYRPCIFEYVYFARPDAIIDGISVYKARLKMGEKLAHKILRDWGEDHDIDVVIPIPDTSRTSALELANILGVK
FREGFMKNRYIGRTFIMPGQQQRKKSVRQKLNPVELEFKGKNVLLVDDSIVRGTTCNEIIQMARDSGAKKVYFASAAPKV
MYPNVYGIDMPAKTELIASERSVEEIQEIIGADRLIFQDLEDLKNAVRTSKVPTLTEFDCSVFDGIYVTGGIDADYLNNL
EQKRNDSAKKKKDGYIDVNIDAASVDLTGIKEE

Sequences:

>Translated_513_residues
MCGVVGIAGKSPVNQMLFDALTMLQHRGQDAAGIVTCHEGRLFLRKDNGMVRDVFHTRHMRALLGNYGIGHVRYPTAGSS
SSAEAQPFYVNSPYGITLAHNGNLTNAEEIHDDLFKTDLRHMNTDSDSEVLLNVFAHELQKIGTLNPTPEDIFHTVSRVH
ERCQGAYGVVAMITGHGLVGFRDPNGIRPLIYGSRVTEQGEMEYIIASESVAITALGFKIERDIAPGEAIFINADGELFT
KQCAADPKYRPCIFEYVYFARPDAIIDGISVYKARLKMGEKLAHKILRDWGEDHDIDVVIPIPDTSRTSALELANILGVK
FREGFMKNRYIGRTFIMPGQQQRKKSVRQKLNPVELEFKGKNVLLVDDSIVRGTTCNEIIQMARDSGAKKVYFASAAPKV
MYPNVYGIDMPAKTELIASERSVEEIQEIIGADRLIFQDLEDLKNAVRTSKVPTLTEFDCSVFDGIYVTGGIDADYLNNL
EQKRNDSAKKKKDGYIDVNIDAASVDLTGIKEE
>Mature_513_residues
MCGVVGIAGKSPVNQMLFDALTMLQHRGQDAAGIVTCHEGRLFLRKDNGMVRDVFHTRHMRALLGNYGIGHVRYPTAGSS
SSAEAQPFYVNSPYGITLAHNGNLTNAEEIHDDLFKTDLRHMNTDSDSEVLLNVFAHELQKIGTLNPTPEDIFHTVSRVH
ERCQGAYGVVAMITGHGLVGFRDPNGIRPLIYGSRVTEQGEMEYIIASESVAITALGFKIERDIAPGEAIFINADGELFT
KQCAADPKYRPCIFEYVYFARPDAIIDGISVYKARLKMGEKLAHKILRDWGEDHDIDVVIPIPDTSRTSALELANILGVK
FREGFMKNRYIGRTFIMPGQQQRKKSVRQKLNPVELEFKGKNVLLVDDSIVRGTTCNEIIQMARDSGAKKVYFASAAPKV
MYPNVYGIDMPAKTELIASERSVEEIQEIIGADRLIFQDLEDLKNAVRTSKVPTLTEFDCSVFDGIYVTGGIDADYLNNL
EQKRNDSAKKKKDGYIDVNIDAASVDLTGIKEE

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=476, Percent_Identity=34.2436974789916, Blast_Score=244, Evalue=1e-64,
Organism=Escherichia coli, GI1788651, Length=490, Percent_Identity=63.6734693877551, Blast_Score=642, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=216, Percent_Identity=30.5555555555556, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=468, Percent_Identity=32.2649572649573, Blast_Score=227, Evalue=9e-60,
Organism=Saccharomyces cerevisiae, GI6323958, Length=507, Percent_Identity=53.8461538461538, Blast_Score=535, Evalue=1e-153,
Organism=Drosophila melanogaster, GI24659598, Length=481, Percent_Identity=33.4719334719335, Blast_Score=253, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24659604, Length=481, Percent_Identity=33.2640332640333, Blast_Score=250, Evalue=2e-66,
Organism=Drosophila melanogaster, GI28573187, Length=473, Percent_Identity=33.615221987315, Blast_Score=240, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 57031; Mature: 57031

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGVVGIAGKSPVNQMLFDALTMLQHRGQDAAGIVTCHEGRLFLRKDNGMVRDVFHTRHM
CCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHH
RALLGNYGIGHVRYPTAGSSSSAEAQPFYVNSPYGITLAHNGNLTNAEEIHDDLFKTDLR
HHHHCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHH
HMNTDSDSEVLLNVFAHELQKIGTLNPTPEDIFHTVSRVHERCQGAYGVVAMITGHGLVG
HCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEE
FRDPNGIRPLIYGSRVTEQGEMEYIIASESVAITALGFKIERDIAPGEAIFINADGELFT
ECCCCCCCCEEECCCCCCCCCEEEEEECCCEEEEEEEEEEECCCCCCCEEEEECCCHHHH
KQCAADPKYRPCIFEYVYFARPDAIIDGISVYKARLKMGEKLAHKILRDWGEDHDIDVVI
HHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
PIPDTSRTSALELANILGVKFREGFMKNRYIGRTFIMPGQQQRKKSVRQKLNPVELEFKG
ECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCEEEEECC
KNVLLVDDSIVRGTTCNEIIQMARDSGAKKVYFASAAPKVMYPNVYGIDMPAKTELIASE
CEEEEECCCEECCCCHHHHHHHHHCCCCCEEEEECCCCCEECCCEEECCCCCCHHHHHHH
RSVEEIQEIIGADRLIFQDLEDLKNAVRTSKVPTLTEFDCSVFDGIYVTGGIDADYLNNL
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHCCEEEECCCCHHHHHHH
EQKRNDSAKKKKDGYIDVNIDAASVDLTGIKEE
HHHHCCHHHHHCCCEEEEEEEEEEEEECCCCCC
>Mature Secondary Structure
MCGVVGIAGKSPVNQMLFDALTMLQHRGQDAAGIVTCHEGRLFLRKDNGMVRDVFHTRHM
CCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHH
RALLGNYGIGHVRYPTAGSSSSAEAQPFYVNSPYGITLAHNGNLTNAEEIHDDLFKTDLR
HHHHCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHH
HMNTDSDSEVLLNVFAHELQKIGTLNPTPEDIFHTVSRVHERCQGAYGVVAMITGHGLVG
HCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEE
FRDPNGIRPLIYGSRVTEQGEMEYIIASESVAITALGFKIERDIAPGEAIFINADGELFT
ECCCCCCCCEEECCCCCCCCCEEEEEECCCEEEEEEEEEEECCCCCCCEEEEECCCHHHH
KQCAADPKYRPCIFEYVYFARPDAIIDGISVYKARLKMGEKLAHKILRDWGEDHDIDVVI
HHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
PIPDTSRTSALELANILGVKFREGFMKNRYIGRTFIMPGQQQRKKSVRQKLNPVELEFKG
ECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCEEEEECC
KNVLLVDDSIVRGTTCNEIIQMARDSGAKKVYFASAAPKVMYPNVYGIDMPAKTELIASE
CEEEEECCCEECCCCHHHHHHHHHCCCCCEEEEECCCCCEECCCEEECCCCCCHHHHHHH
RSVEEIQEIIGADRLIFQDLEDLKNAVRTSKVPTLTEFDCSVFDGIYVTGGIDADYLNNL
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHCCEEEECCCCHHHHHHH
EQKRNDSAKKKKDGYIDVNIDAASVDLTGIKEE
HHHHCCHHHHHCCCEEEEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]