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The map label for this gene is alsR [H]

Identifier: 213158624

GI number: 213158624

Start: 2669594

End: 2670496

Strand: Direct

Name: alsR [H]

Synonym: AB57_2578

Alternate gene names: 213158624

Gene position: 2669594-2670496 (Clockwise)

Preceding gene: 213158620

Following gene: 213158625

Centisome position: 65.91

GC content: 43.3

Gene sequence:

>903_bases
ATGATCGATTTACGCCAATTGAAGTATTTTGTTGTAGTGGCTGAAGAAGAACACGTTGGCCGTGCAGCTGAACGCTTATT
TATCTCCCAGTCTCCACTTAGCCGTCAGATTTCTCAGTTAGAAGAAAAGCTTGGTCTCATGTTGTTTGAACGGAACCAAC
AACGTATTCGTTTAACTGCCGATGGTCAGACATTTTTAGCTGAAGCTCGTGCTTTACTCACCCATGCCAACCGTTTGGAA
TCTTTAGGCAAACGCCTTGGACGTGGTGAAGAAGGCGGTTTATGTATTGGTTATATTGAAAATGCAATGCATTCAGGTGT
GTTGTCTAATGCTTTACGCTCGTTACGTCAGTCCAGACCAGATGTACATATCGCACTTTATAACCAGACTAGTCCCATCC
AACTTGAAGGTTTGAAACAGCGTAGTCTTGATATTGCCATGGTCAACTGTCCACTAGAGCTTGAAGACCCTGACTTAGGC
TATACACAAGTTTTAGATGATCGCATGTTGCTTGCTTTACCTGAACAGCATCCATTAGTAAGTAAAGCTGAGCTTGAAGC
AAGTGACCTTGAAGGTGTCGAATGGATTTCTGTCATTCATGAGGAAGATGTCCATTCTCGTGATGATTTTATTAGCGCTT
GTGCCAAAGCTGGTTTTACACCAGACATTAAACTGGAAGCTGGAGCACCTTTAACGGCTTTAGGTCTGGTTGCAGCAGGT
TTAGGACTCACCTTTATTCAACATAGTTTGCGACATCACGTTCCTGATGGGGTAGTACTTCGCGAACTCCCATGGATGTC
TTACAACACTCAACTTTGGGCTGTATGGCATAAGGCAAACTTACGTCCGCTGGTGGTCCATTTTAGGCAAATGTTGACCA
ATGAATTTACGGTAGAAGAATAA

Upstream 100 bases:

>100_bases
GCAGTTTTTCGGGCTGCCTTTGCGATGAAGAAAATATAGCTTGATCACTTGTGACATGATAGGACATAATTCGACTGACT
TGAGTCCCTTAAGGTCTTAT

Downstream 100 bases:

>100_bases
ATTCTATAGCTATTAAATATTGATTAACTAAGGCACCTCGTGTGCTTTAGTTAACCAAAATCAATAATCTTATTTTTATT
ATTGACTAATACTCAATAAT

Product: transcriptional regulator, LysR family

Products: NA

Alternate protein names: Als operon regulatory protein [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MIDLRQLKYFVVVAEEEHVGRAAERLFISQSPLSRQISQLEEKLGLMLFERNQQRIRLTADGQTFLAEARALLTHANRLE
SLGKRLGRGEEGGLCIGYIENAMHSGVLSNALRSLRQSRPDVHIALYNQTSPIQLEGLKQRSLDIAMVNCPLELEDPDLG
YTQVLDDRMLLALPEQHPLVSKAELEASDLEGVEWISVIHEEDVHSRDDFISACAKAGFTPDIKLEAGAPLTALGLVAAG
LGLTFIQHSLRHHVPDGVVLRELPWMSYNTQLWAVWHKANLRPLVVHFRQMLTNEFTVEE

Sequences:

>Translated_300_residues
MIDLRQLKYFVVVAEEEHVGRAAERLFISQSPLSRQISQLEEKLGLMLFERNQQRIRLTADGQTFLAEARALLTHANRLE
SLGKRLGRGEEGGLCIGYIENAMHSGVLSNALRSLRQSRPDVHIALYNQTSPIQLEGLKQRSLDIAMVNCPLELEDPDLG
YTQVLDDRMLLALPEQHPLVSKAELEASDLEGVEWISVIHEEDVHSRDDFISACAKAGFTPDIKLEAGAPLTALGLVAAG
LGLTFIQHSLRHHVPDGVVLRELPWMSYNTQLWAVWHKANLRPLVVHFRQMLTNEFTVEE
>Mature_300_residues
MIDLRQLKYFVVVAEEEHVGRAAERLFISQSPLSRQISQLEEKLGLMLFERNQQRIRLTADGQTFLAEARALLTHANRLE
SLGKRLGRGEEGGLCIGYIENAMHSGVLSNALRSLRQSRPDVHIALYNQTSPIQLEGLKQRSLDIAMVNCPLELEDPDLG
YTQVLDDRMLLALPEQHPLVSKAELEASDLEGVEWISVIHEEDVHSRDDFISACAKAGFTPDIKLEAGAPLTALGLVAAG
LGLTFIQHSLRHHVPDGVVLRELPWMSYNTQLWAVWHKANLRPLVVHFRQMLTNEFTVEE

Specific function: Regulates the expression of the alsSD operon for acetoin biosynthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=299, Percent_Identity=32.1070234113712, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI2367136, Length=295, Percent_Identity=31.864406779661, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1788887, Length=292, Percent_Identity=30.4794520547945, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1790399, Length=248, Percent_Identity=30.6451612903226, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI145693105, Length=260, Percent_Identity=25.7692307692308, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788748, Length=264, Percent_Identity=26.5151515151515, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI87081904, Length=215, Percent_Identity=30.6976744186047, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI157672245, Length=237, Percent_Identity=27.4261603375527, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787806, Length=289, Percent_Identity=24.5674740484429, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787530, Length=248, Percent_Identity=25.8064516129032, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI145693193, Length=232, Percent_Identity=25.4310344827586, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788297, Length=147, Percent_Identity=27.891156462585, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788296, Length=248, Percent_Identity=26.2096774193548, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789204, Length=261, Percent_Identity=26.8199233716475, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790262, Length=253, Percent_Identity=26.4822134387352, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI48994962, Length=73, Percent_Identity=45.2054794520548, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1790208, Length=258, Percent_Identity=27.1317829457364, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33735; Mature: 33735

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLRQLKYFVVVAEEEHVGRAAERLFISQSPLSRQISQLEEKLGLMLFERNQQRIRLTA
CCCHHEEEEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEECCCCEEEEEE
DGQTFLAEARALLTHANRLESLGKRLGRGEEGGLCIGYIENAMHSGVLSNALRSLRQSRP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCC
DVHIALYNQTSPIQLEGLKQRSLDIAMVNCPLELEDPDLGYTQVLDDRMLLALPEQHPLV
CEEEEEECCCCCEEECCCCCCCCEEEEEECCEEECCCCCCHHHHHCCCCEEECCCCCCCC
SKAELEASDLEGVEWISVIHEEDVHSRDDFISACAKAGFTPDIKLEAGAPLTALGLVAAG
CHHHCCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHH
LGLTFIQHSLRHHVPDGVVLRELPWMSYNTQLWAVWHKANLRPLVVHFRQMLTNEFTVEE
HHHHHHHHHHHHCCCCCEEEHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIDLRQLKYFVVVAEEEHVGRAAERLFISQSPLSRQISQLEEKLGLMLFERNQQRIRLTA
CCCHHEEEEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEECCCCEEEEEE
DGQTFLAEARALLTHANRLESLGKRLGRGEEGGLCIGYIENAMHSGVLSNALRSLRQSRP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCC
DVHIALYNQTSPIQLEGLKQRSLDIAMVNCPLELEDPDLGYTQVLDDRMLLALPEQHPLV
CEEEEEECCCCCEEECCCCCCCCEEEEEECCEEECCCCCCHHHHHCCCCEEECCCCCCCC
SKAELEASDLEGVEWISVIHEEDVHSRDDFISACAKAGFTPDIKLEAGAPLTALGLVAAG
CHHHCCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHH
LGLTFIQHSLRHHVPDGVVLRELPWMSYNTQLWAVWHKANLRPLVVHFRQMLTNEFTVEE
HHHHHHHHHHHHCCCCCEEEHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377 [H]