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The map label for this gene is proP [H]

Identifier: 213158607

GI number: 213158607

Start: 2654436

End: 2655923

Strand: Direct

Name: proP [H]

Synonym: AB57_2561

Alternate gene names: 213158607

Gene position: 2654436-2655923 (Clockwise)

Preceding gene: 213158599

Following gene: 213158608

Centisome position: 65.53

GC content: 39.31

Gene sequence:

>1488_bases
ATGCGCACATCTGAAAAACAAGTAAAACCTATTGAGATTGATGATATCAATATCATCGAGAACGAAAAAACCAAAAAAGC
GATTACTGCAGCTGCATTAGGTAATGCAATTGAATGGTTTGATTTCGGAGTTTATGGTTATGTGGCATACGTTCTCGGTA
AAGTATTTTTCCCAGATGCTTCACCAAGCGTGCAGATGATTGCCGCGCTTGCTACATTTTCAGTACCTTTTATTTTCCGT
CCATTAGGTGGTCTTTTCTTTGGACATTTAGGTGACAAATACGGACGTCAAAAAGTTCTGGCCATTACTGTCATTATTAT
GTCCATCAGTACCTTTGGTATTGGCCTTATTCCATCTTATGAAACAATCGGTTTATGGGCACCTATTCTTTTACTCATCG
TAAAAATTGTACAAGGCTTCTCCATTGGTGGTGAATACTCAGGTGCTGCGATTTTTGTAGCTGAATATTCTCCAGACCGA
AAACGTGGCTTTATGGGAAGCTGGCTTGATTTTGGATCGATAGCCGGCTTTGTACTTGGGGCTGCAACTGTCGCCTTGAT
CACACACGCCGTGGGTGAGGCACGTTTTGCCGAGTGGGGTTGGCGTATTCCATTCTTTCTCGCTCTACCACTGGGCATAA
TCGGTTTATATTTACGAAACCGCCTTGAAGAAACCCCTGTATATCAACAGCATTCAGAGCAACAAGCACAAAAAAGCAAA
CCTCAAAAATTTAGTTTCAAAGAAATTTTTGTTAAACATAAGCGTAGTTTATTGGTGTGCATTGGCCTCGTAATCAGTAC
TAACGTTACTTACTACATGTTGCTAACTTATTTACCAAGCTATTTTTCGCATAACTTAGGCTATTCAGAAGCACATGGTG
CACTTATTATCATTGCGGTTATGGTTGGCATGTTATTTGTACAACCTGTGATTGGTTATTTGAGTGATAAGTTCGGACGT
CGCCCATTTATTTTTATTGGAAGCTTTTCTCTTATTTTCTTGTCCTATCCTGCCTTTGTATTACTTAACTCTGGGGTAAA
TTATCAAATTTTTATAGGCTTACTTATTCTTGCTTTATCTCTCAATATGTCTATAGGAGTGATGGCATCAACTTTACCTG
CCCTATTTCCAACTGAAATCCGTTATAGCGCTTTAGGTATTGCCTTTAATTTCTCAGTGGTTATTGCTGGTTTAACCCCA
ACTCTTACCGCAACGCTGGTTGAAACTACCCACAATCTGATGGTTCCAGCCTATTACTTGATGATTTGCGGGGTGGTAGG
TATTGCTACTGCAATGTATTTAAAGGAAACAGCCAATAAGCCTTTAATCGGGGGCGTGCCTATTGCTCACAATATAGAGG
AAGCACAAGAGTTACTTGAAGAGTTTCATGAAACTATTGAACAGAAAATTGAAAATATTGATCAGAAAATTCAGGAGCTT
CAACAAAAACGTCAAAAACTTGCTGATAAACACCCTGATATAGCATGA

Upstream 100 bases:

>100_bases
CTTATCGTTATTCTGTCTCTTTATTCTGCAATTTTTTAATTCAGTATTTAAAACCGTATATTTTCACTTTAAATACTGAG
ATTCAATTCTTAGGCTAGAT

Downstream 100 bases:

>100_bases
GTAAAGCAACGTTTTTTATAAGAGTAGCGACTTAAATGTCGCTATTCTTTTAATATATTTTTCATTCAATTTGATAGTAA
AAAAGAATTAACTTAGTAAA

Product: proline/glycine betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MRTSEKQVKPIEIDDINIIENEKTKKAITAAALGNAIEWFDFGVYGYVAYVLGKVFFPDASPSVQMIAALATFSVPFIFR
PLGGLFFGHLGDKYGRQKVLAITVIIMSISTFGIGLIPSYETIGLWAPILLLIVKIVQGFSIGGEYSGAAIFVAEYSPDR
KRGFMGSWLDFGSIAGFVLGAATVALITHAVGEARFAEWGWRIPFFLALPLGIIGLYLRNRLEETPVYQQHSEQQAQKSK
PQKFSFKEIFVKHKRSLLVCIGLVISTNVTYYMLLTYLPSYFSHNLGYSEAHGALIIIAVMVGMLFVQPVIGYLSDKFGR
RPFIFIGSFSLIFLSYPAFVLLNSGVNYQIFIGLLILALSLNMSIGVMASTLPALFPTEIRYSALGIAFNFSVVIAGLTP
TLTATLVETTHNLMVPAYYLMICGVVGIATAMYLKETANKPLIGGVPIAHNIEEAQELLEEFHETIEQKIENIDQKIQEL
QQKRQKLADKHPDIA

Sequences:

>Translated_495_residues
MRTSEKQVKPIEIDDINIIENEKTKKAITAAALGNAIEWFDFGVYGYVAYVLGKVFFPDASPSVQMIAALATFSVPFIFR
PLGGLFFGHLGDKYGRQKVLAITVIIMSISTFGIGLIPSYETIGLWAPILLLIVKIVQGFSIGGEYSGAAIFVAEYSPDR
KRGFMGSWLDFGSIAGFVLGAATVALITHAVGEARFAEWGWRIPFFLALPLGIIGLYLRNRLEETPVYQQHSEQQAQKSK
PQKFSFKEIFVKHKRSLLVCIGLVISTNVTYYMLLTYLPSYFSHNLGYSEAHGALIIIAVMVGMLFVQPVIGYLSDKFGR
RPFIFIGSFSLIFLSYPAFVLLNSGVNYQIFIGLLILALSLNMSIGVMASTLPALFPTEIRYSALGIAFNFSVVIAGLTP
TLTATLVETTHNLMVPAYYLMICGVVGIATAMYLKETANKPLIGGVPIAHNIEEAQELLEEFHETIEQKIENIDQKIQEL
QQKRQKLADKHPDIA
>Mature_495_residues
MRTSEKQVKPIEIDDINIIENEKTKKAITAAALGNAIEWFDFGVYGYVAYVLGKVFFPDASPSVQMIAALATFSVPFIFR
PLGGLFFGHLGDKYGRQKVLAITVIIMSISTFGIGLIPSYETIGLWAPILLLIVKIVQGFSIGGEYSGAAIFVAEYSPDR
KRGFMGSWLDFGSIAGFVLGAATVALITHAVGEARFAEWGWRIPFFLALPLGIIGLYLRNRLEETPVYQQHSEQQAQKSK
PQKFSFKEIFVKHKRSLLVCIGLVISTNVTYYMLLTYLPSYFSHNLGYSEAHGALIIIAVMVGMLFVQPVIGYLSDKFGR
RPFIFIGSFSLIFLSYPAFVLLNSGVNYQIFIGLLILALSLNMSIGVMASTLPALFPTEIRYSALGIAFNFSVVIAGLTP
TLTATLVETTHNLMVPAYYLMICGVVGIATAMYLKETANKPLIGGVPIAHNIEEAQELLEEFHETIEQKIENIDQKIQEL
QQKRQKLADKHPDIA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=496, Percent_Identity=69.1532258064516, Blast_Score=714, Evalue=0.0,
Organism=Escherichia coli, GI1788292, Length=429, Percent_Identity=32.4009324009324, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI1788942, Length=386, Percent_Identity=32.3834196891192, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1789941, Length=427, Percent_Identity=32.0843091334895, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI87082404, Length=407, Percent_Identity=26.5356265356265, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI87082231, Length=442, Percent_Identity=24.2081447963801, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786229, Length=337, Percent_Identity=26.1127596439169, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 54726; Mature: 54726

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSEKQVKPIEIDDINIIENEKTKKAITAAALGNAIEWFDFGVYGYVAYVLGKVFFPDA
CCCCCCCCCCEEECCCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
SPSVQMIAALATFSVPFIFRPLGGLFFGHLGDKYGRQKVLAITVIIMSISTFGIGLIPSY
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHCEECCCCC
ETIGLWAPILLLIVKIVQGFSIGGEYSGAAIFVAEYSPDRKRGFMGSWLDFGSIAGFVLG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHCCCCCCHHHHHHHHHHHHH
AATVALITHAVGEARFAEWGWRIPFFLALPLGIIGLYLRNRLEETPVYQQHSEQQAQKSK
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
PQKFSFKEIFVKHKRSLLVCIGLVISTNVTYYMLLTYLPSYFSHNLGYSEAHGALIIIAV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHH
MVGMLFVQPVIGYLSDKFGRRPFIFIGSFSLIFLSYPAFVLLNSGVNYQIFIGLLILALS
HHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHH
LNMSIGVMASTLPALFPTEIRYSALGIAFNFSVVIAGLTPTLTATLVETTHNLMVPAYYL
HCCHHHHHHHHHHHHCCCHHHHHHHEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
MICGVVGIATAMYLKETANKPLIGGVPIAHNIEEAQELLEEFHETIEQKIENIDQKIQEL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQKRQKLADKHPDIA
HHHHHHHHCCCCCCC
>Mature Secondary Structure
MRTSEKQVKPIEIDDINIIENEKTKKAITAAALGNAIEWFDFGVYGYVAYVLGKVFFPDA
CCCCCCCCCCEEECCCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
SPSVQMIAALATFSVPFIFRPLGGLFFGHLGDKYGRQKVLAITVIIMSISTFGIGLIPSY
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHCEECCCCC
ETIGLWAPILLLIVKIVQGFSIGGEYSGAAIFVAEYSPDRKRGFMGSWLDFGSIAGFVLG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHCCCCCCHHHHHHHHHHHHH
AATVALITHAVGEARFAEWGWRIPFFLALPLGIIGLYLRNRLEETPVYQQHSEQQAQKSK
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
PQKFSFKEIFVKHKRSLLVCIGLVISTNVTYYMLLTYLPSYFSHNLGYSEAHGALIIIAV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHH
MVGMLFVQPVIGYLSDKFGRRPFIFIGSFSLIFLSYPAFVLLNSGVNYQIFIGLLILALS
HHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHH
LNMSIGVMASTLPALFPTEIRYSALGIAFNFSVVIAGLTPTLTATLVETTHNLMVPAYYL
HCCHHHHHHHHHHHHCCCHHHHHHHEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
MICGVVGIATAMYLKETANKPLIGGVPIAHNIEEAQELLEEFHETIEQKIENIDQKIQEL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQKRQKLADKHPDIA
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]