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The map label for this gene is thiM

Identifier: 213158478

GI number: 213158478

Start: 2514719

End: 2515546

Strand: Direct

Name: thiM

Synonym: AB57_2427

Alternate gene names: 213158478

Gene position: 2514719-2515546 (Clockwise)

Preceding gene: 213158477

Following gene: 213158481

Centisome position: 62.08

GC content: 41.18

Gene sequence:

>828_bases
ATGAATTCGACATCCAACCTCATTGAACAGGTGATTGAAGCTTGGCAAAACATGCAGGCAAAAACGCCGCTGGTACAATG
TATTACTAACAGTGTTGCTGCCAACTATACAGCCAATGTATTACTTGCTTCCGGTGCTTCACCTGCCATGATTGATAATC
CTTATGAAGCAGAAAGCTTTACCAAAATTTCATCGGCTTTAAGCATTAATTTAGGCACACCGACTTCAGAGCAAATGCAA
GCCATGCAAATCTCTGCCAAGACCGCACAGCTTAACAATGTTCCTTGGGTACTCGATCCGGTAGGCTATGGACCAATATT
AGCTTGGCGCAGTCAAATGACAGACGAGCTTTTACAGTTCAAGCCAAGCGTTATACGTGGTAATGCCTCAGAAATCAGTA
CGCTTGCAGGCAATCAGGTTCAATCAAAAGGTGTAGACAGCACTTTAAGCAGTGACCAAGCTTATCAACAAGCTTTTTCA
CTATTAACTCATGCAAGCTGTATCGCTATTTCAGGTGAATCAGATTACATTTTGTCTAACGAAGTTGATGCTGTAATTCA
AGTAAATGGTGGAAGCCCGTTACAACCCAAAATTACTGCAACTGGCTGCGCTTTGGGTGCATTAATTGCAGCATATAGCG
CTGTGACGACACCAACCATTGCAGCTCTTTCAGCTCATATTCATTTTGCAATTGCAGGTAAACTTGCTGCAAATCAAGCT
CAAACGATGGGTAGTTTTAGTAGCATATTTATGGATTATATCCATATGTTAGATGCTAATCTGATTGAGCAATACGCAGA
TGTCAAACTTTTAAATAAACAGGCATAA

Upstream 100 bases:

>100_bases
TGCAGCAGATTATGTGTCGTTGATTTGCCAAAATTGCAAACATCAAGATCACTATTTTACCCGTTAACTTTCTTATCTCT
GGCTTAATAAGGATTATCAG

Downstream 100 bases:

>100_bases
CCTAAACTATGCCTGAATTACACTAGACTCACTTTAAAAGTAAGTAGATGTCGGTAATAAGATCAGACTACTTTTACATA
GTGCTCATTGCTATTCTTGA

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MNSTSNLIEQVIEAWQNMQAKTPLVQCITNSVAANYTANVLLASGASPAMIDNPYEAESFTKISSALSINLGTPTSEQMQ
AMQISAKTAQLNNVPWVLDPVGYGPILAWRSQMTDELLQFKPSVIRGNASEISTLAGNQVQSKGVDSTLSSDQAYQQAFS
LLTHASCIAISGESDYILSNEVDAVIQVNGGSPLQPKITATGCALGALIAAYSAVTTPTIAALSAHIHFAIAGKLAANQA
QTMGSFSSIFMDYIHMLDANLIEQYADVKLLNKQA

Sequences:

>Translated_275_residues
MNSTSNLIEQVIEAWQNMQAKTPLVQCITNSVAANYTANVLLASGASPAMIDNPYEAESFTKISSALSINLGTPTSEQMQ
AMQISAKTAQLNNVPWVLDPVGYGPILAWRSQMTDELLQFKPSVIRGNASEISTLAGNQVQSKGVDSTLSSDQAYQQAFS
LLTHASCIAISGESDYILSNEVDAVIQVNGGSPLQPKITATGCALGALIAAYSAVTTPTIAALSAHIHFAIAGKLAANQA
QTMGSFSSIFMDYIHMLDANLIEQYADVKLLNKQA
>Mature_275_residues
MNSTSNLIEQVIEAWQNMQAKTPLVQCITNSVAANYTANVLLASGASPAMIDNPYEAESFTKISSALSINLGTPTSEQMQ
AMQISAKTAQLNNVPWVLDPVGYGPILAWRSQMTDELLQFKPSVIRGNASEISTLAGNQVQSKGVDSTLSSDQAYQQAFS
LLTHASCIAISGESDYILSNEVDAVIQVNGGSPLQPKITATGCALGALIAAYSAVTTPTIAALSAHIHFAIAGKLAANQA
QTMGSFSSIFMDYIHMLDANLIEQYADVKLLNKQA

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=236, Percent_Identity=41.5254237288136, Blast_Score=166, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6325042, Length=283, Percent_Identity=32.5088339222615, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_ACIB3 (B7H160)

Other databases:

- EMBL:   CP001172
- RefSeq:   YP_002325274.1
- ProteinModelPortal:   B7H160
- SMR:   B7H160
- GeneID:   7058113
- GenomeReviews:   CP001172_GR
- KEGG:   abb:ABBFA_001371
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- ProtClustDB:   CLSK707428
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 29148; Mature: 29148

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: BINDING 50-50 BINDING 126-126 BINDING 171-171 BINDING 200-200

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTSNLIEQVIEAWQNMQAKTPLVQCITNSVAANYTANVLLASGASPAMIDNPYEAESF
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHH
TKISSALSINLGTPTSEQMQAMQISAKTAQLNNVPWVLDPVGYGPILAWRSQMTDELLQF
HHHHHHEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHC
KPSVIRGNASEISTLAGNQVQSKGVDSTLSSDQAYQQAFSLLTHASCIAISGESDYILSN
CCHHHCCCHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEECC
EVDAVIQVNGGSPLQPKITATGCALGALIAAYSAVTTPTIAALSAHIHFAIAGKLAANQA
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEECHHHHHHH
QTMGSFSSIFMDYIHMLDANLIEQYADVKLLNKQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNSTSNLIEQVIEAWQNMQAKTPLVQCITNSVAANYTANVLLASGASPAMIDNPYEAESF
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHH
TKISSALSINLGTPTSEQMQAMQISAKTAQLNNVPWVLDPVGYGPILAWRSQMTDELLQF
HHHHHHEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHC
KPSVIRGNASEISTLAGNQVQSKGVDSTLSSDQAYQQAFSLLTHASCIAISGESDYILSN
CCHHHCCCHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEECC
EVDAVIQVNGGSPLQPKITATGCALGALIAAYSAVTTPTIAALSAHIHFAIAGKLAANQA
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEECHHHHHHH
QTMGSFSSIFMDYIHMLDANLIEQYADVKLLNKQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA