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The map label for this gene is mscM [H]

Identifier: 213158458

GI number: 213158458

Start: 2493388

End: 2494560

Strand: Reverse

Name: mscM [H]

Synonym: AB57_2407

Alternate gene names: 213158458

Gene position: 2494560-2493388 (Counterclockwise)

Preceding gene: 213158460

Following gene: 213158457

Centisome position: 61.59

GC content: 38.19

Gene sequence:

>1173_bases
ATGCTAGTTTCGCTCACACTTTTGATCCTTTTTGCTGCATTAGCAAATTTCATTGCTAAACGAATTATTGTTCGGGGAAT
ACGTCATTTAGTGACTAAAATTAAGTCACTCAACCAGTCGGTTTTTGCCCAGCATAGTGTCATTAAGCGATTTGCCAATA
TTGTGCCGGCCATTGTCATTATGAACGGTATTACTACCGTTCCGCATTTGTCGGAAAAAGCGGTTGCTTTGGTACAGATG
GGCGCACAGGCATTTATTTTTCTCACGATTGCGCTCACCATTAGTGAATTATTAAATATATTTAATTTAATCTATCAGCG
TAATCCAAAATCTAGAAACAAGCCGATTAAAGGTTACTTGCAACTCGTTAAACTCATTATTTTTGTTGTTTGCGGATTAA
TGATTTTGGGTACATTCCTGAAAAAGGATGTATTTACGTTGTTGGCTGGCTTCGGTGCAATGGCAGCCGTATTAATGTTA
GTTTTCCAAAATACAATTTTGTCATTGGTTGCGAGTGTACAAATTGCCTCTTATGACATGGTACGTATTGGGGACTGGAT
TGAAATGCCATCGCTCAATGCTGATGGTGATGTCATTGATATTTCATTGCACACAGTGACCGTTCAAAACTTTGATAAAA
CATACACCACGATTCCAACCAATAAACTAGTGACAGACACTTTTAAAAACTGGCGTGGTATGAGTAATGCGGGCTGTCGC
CGTATGAAGCGTTCTCTTTATATCGATCAAACTAGCGTGCACTTCATGACCGAAGAAGAGCAACAAAAACTCAAAGATTT
TTTATTGCTAGATCAATACCTCAATGCCAAGCAATCTGAGATTGACGAATTTAATAAGCATTTAGGTAGTCAGTCACGTT
ATAACAAAAGACGCTTAACCAATATTGGAACATTTAGAGCATATGTTGAATTCTATTTGCGCCAGCATAAGGGGATTGCT
CAAAATCAAACGCTTATTGTTCGCCAACTACAACCAACGAGTGAAGGTTTACCGTTAGAAATCTATGCATTTACCAATAC
CACTGCATGGGTTTCTTATGAAGCAATTCAGTCCGATATTTTTGATCATCTGATTGCGATTTTGCCTGAGTTTGGCTTAA
GAGTGTATCAGGCACCTTCAGGCCATGATTTTCAGAAATTAACAGTAGAATAA

Upstream 100 bases:

>100_bases
TTAAAATTATTGAAATAAAGTTTTAAGGTGTAGGTTTTGGATTTTTTTAATTTTTTATCGTGATCTTCAAGCTGAGTTAA
GCAACTATCCTTGGCTAGAA

Downstream 100 bases:

>100_bases
ACAACTGTTAGCGTGCTATCTATTTTATTTTCAAATAGATAGCATCTTTTTAATAATTTTATACATTAATTTATAACTAT
TCTTAGAAAATTATTATTAA

Product: mechanosensitive ion channel family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MLVSLTLLILFAALANFIAKRIIVRGIRHLVTKIKSLNQSVFAQHSVIKRFANIVPAIVIMNGITTVPHLSEKAVALVQM
GAQAFIFLTIALTISELLNIFNLIYQRNPKSRNKPIKGYLQLVKLIIFVVCGLMILGTFLKKDVFTLLAGFGAMAAVLML
VFQNTILSLVASVQIASYDMVRIGDWIEMPSLNADGDVIDISLHTVTVQNFDKTYTTIPTNKLVTDTFKNWRGMSNAGCR
RMKRSLYIDQTSVHFMTEEEQQKLKDFLLLDQYLNAKQSEIDEFNKHLGSQSRYNKRRLTNIGTFRAYVEFYLRQHKGIA
QNQTLIVRQLQPTSEGLPLEIYAFTNTTAWVSYEAIQSDIFDHLIAILPEFGLRVYQAPSGHDFQKLTVE

Sequences:

>Translated_390_residues
MLVSLTLLILFAALANFIAKRIIVRGIRHLVTKIKSLNQSVFAQHSVIKRFANIVPAIVIMNGITTVPHLSEKAVALVQM
GAQAFIFLTIALTISELLNIFNLIYQRNPKSRNKPIKGYLQLVKLIIFVVCGLMILGTFLKKDVFTLLAGFGAMAAVLML
VFQNTILSLVASVQIASYDMVRIGDWIEMPSLNADGDVIDISLHTVTVQNFDKTYTTIPTNKLVTDTFKNWRGMSNAGCR
RMKRSLYIDQTSVHFMTEEEQQKLKDFLLLDQYLNAKQSEIDEFNKHLGSQSRYNKRRLTNIGTFRAYVEFYLRQHKGIA
QNQTLIVRQLQPTSEGLPLEIYAFTNTTAWVSYEAIQSDIFDHLIAILPEFGLRVYQAPSGHDFQKLTVE
>Mature_390_residues
MLVSLTLLILFAALANFIAKRIIVRGIRHLVTKIKSLNQSVFAQHSVIKRFANIVPAIVIMNGITTVPHLSEKAVALVQM
GAQAFIFLTIALTISELLNIFNLIYQRNPKSRNKPIKGYLQLVKLIIFVVCGLMILGTFLKKDVFTLLAGFGAMAAVLML
VFQNTILSLVASVQIASYDMVRIGDWIEMPSLNADGDVIDISLHTVTVQNFDKTYTTIPTNKLVTDTFKNWRGMSNAGCR
RMKRSLYIDQTSVHFMTEEEQQKLKDFLLLDQYLNAKQSEIDEFNKHLGSQSRYNKRRLTNIGTFRAYVEFYLRQHKGIA
QNQTLIVRQLQPTSEGLPLEIYAFTNTTAWVSYEAIQSDIFDHLIAILPEFGLRVYQAPSGHDFQKLTVE

Specific function: Mechanosensitive channel of miniconductance that confers protection against mild hypoosmotic shock. Overexpression confers protection against severe shocks [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786791, Length=390, Percent_Identity=43.5897435897436, Blast_Score=332, Evalue=4e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 44213; Mature: 44213

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVSLTLLILFAALANFIAKRIIVRGIRHLVTKIKSLNQSVFAQHSVIKRFANIVPAIVI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNGITTVPHLSEKAVALVQMGAQAFIFLTIALTISELLNIFNLIYQRNPKSRNKPIKGYL
HHCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
QLVKLIIFVVCGLMILGTFLKKDVFTLLAGFGAMAAVLMLVFQNTILSLVASVQIASYDM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VRIGDWIEMPSLNADGDVIDISLHTVTVQNFDKTYTTIPTNKLVTDTFKNWRGMSNAGCR
HEECCCCCCCCCCCCCCEEEEEEEEEEEECCCCCEEECCCCHHHHHHHHHCCCCCHHHHH
RMKRSLYIDQTSVHFMTEEEQQKLKDFLLLDQYLNAKQSEIDEFNKHLGSQSRYNKRRLT
HHHHHHEECCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHH
NIGTFRAYVEFYLRQHKGIAQNQTLIVRQLQPTSEGLPLEIYAFTNTTAWVSYEAIQSDI
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCHHHHHHHHHHHH
FDHLIAILPEFGLRVYQAPSGHDFQKLTVE
HHHHHHHHHHHCCEEEECCCCCCCCEECCC
>Mature Secondary Structure
MLVSLTLLILFAALANFIAKRIIVRGIRHLVTKIKSLNQSVFAQHSVIKRFANIVPAIVI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNGITTVPHLSEKAVALVQMGAQAFIFLTIALTISELLNIFNLIYQRNPKSRNKPIKGYL
HHCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
QLVKLIIFVVCGLMILGTFLKKDVFTLLAGFGAMAAVLMLVFQNTILSLVASVQIASYDM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VRIGDWIEMPSLNADGDVIDISLHTVTVQNFDKTYTTIPTNKLVTDTFKNWRGMSNAGCR
HEECCCCCCCCCCCCCCEEEEEEEEEEEECCCCCEEECCCCHHHHHHHHHCCCCCHHHHH
RMKRSLYIDQTSVHFMTEEEQQKLKDFLLLDQYLNAKQSEIDEFNKHLGSQSRYNKRRLT
HHHHHHEECCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHH
NIGTFRAYVEFYLRQHKGIAQNQTLIVRQLQPTSEGLPLEIYAFTNTTAWVSYEAIQSDI
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCHHHHHHHHHHHH
FDHLIAILPEFGLRVYQAPSGHDFQKLTVE
HHHHHHHHHHHCCEEEECCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7813889 [H]