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The map label for this gene is radA [H]

Identifier: 213158320

GI number: 213158320

Start: 3142114

End: 3143502

Strand: Direct

Name: radA [H]

Synonym: AB57_3048

Alternate gene names: 213158320

Gene position: 3142114-3143502 (Clockwise)

Preceding gene: 213158314

Following gene: 213158321

Centisome position: 77.57

GC content: 45.5

Gene sequence:

>1389_bases
ATGAGCAAAGTCAAAACCGTTTACCGTTGCGAGCAATGTGGTGCCGATCATCTAAAATGGGCAGGTCAATGTTCAGAGTG
TGGTGAATGGAATTGTCTTAATGAAGTCAAGCTAGAACCCACTACTGCTCATCGTGCACGTCCTAAAGTTGGGGGTTATG
CGGGTCAAGTTGCTAACATTACAACGCTTAATAAAGTCTCTGTTTCTCATGAAACCCGTTTACCAACAGGCATTAGCGAA
TTTGACCGTGTGCTTGGTGGTGGCTTGGTGACAGGTTCTGTAGTACTCATTGGTGGTGACCCGGGTATTGGTAAATCGAC
CATTCTTTTACAAACAGCAACTCACATGGCAAGTGCAAAAAGTTCTGCACTTTATATTACGGGTGAGGAATCGCTGTCAC
AGGTTGCCTTACGCGCCCAACGCCTTGACCTACCAACAGACCAGTTAAAAGTCATGGCTGAAACTTGTGTCGAACGCATT
TGTGAAGTTTTAGAACAAGAACGTCCTGTTGTCGCAATCTTGGACTCTATCCAAACGCTTTACACAGAAACGCTTCAATC
TGCGCCGGGTGGGGTTTCACAAATTCGTGAATCTGCTGCGTTATTAACCCGTTATGCAAAAAATAGTGGCACAGCGCTAT
TTATTGTTGGCCATGTGACCAAAGAAGGTGCACTGGCAGGTCCGCGTGTTTTAGAGCATATGGTGGACTGCGTACTTTAC
TTTGAAGGTCAATCAGACTCCCGCTACCGTATGATTCGTGCTGTTAAAAACCGTTTTGGTGCAGTGAATGAGCTTGGCGT
ATTTGGCATGACTGATAAAGGCCTACGGGAAGTGGCCAATCCTTCTGCTATTTTCTTAAGTCGTTATGATGAAGCGATTC
CCGGCTCAATTGTCATGATTAGTCGTGAAGGAACTCGCCCTCTTTTGGTCGAGGTACAAGCTTTAGTTGATGATGCTCAA
GGACAACCACGACGTGTTGCACTTGGTCTTGAACAAAACCGTTTAAACATGCTGCTTGCTGTTATGCATCGTCATGGTGG
TGTACAAACCACCGGACAAGATGTGTATGTCAATATTGTAGGTGGTTTAAAAATTACCGAAACAGGTTCGGACTTGGCTG
TTTTACTTGCATGTGCTTCGAGTTTACGAACAAAAGCCTTACCTCAGCAGCTTGCAGTTTTTGGTGAAGTTGGGCTCTCA
GGTGAAATCCGCCCTGTACCTAATGGTCAGGAACGTTTAAAAGAAGCAGCAAAACATGGGTTTAAATACGTCATCTTACC
AAGAGGTAACGCCCCGCAAAAAGCAATTCCGGGCGTACAAGTGATTGCTGTTGCCCGCCTGCATGAAGCCTTGACTGAGG
CGATGCAACTTAGTGATGAATTGACATAA

Upstream 100 bases:

>100_bases
AGCTTAAGCAAAATGTATTCCATATCGATTAAGTTCTCTATTGGGCTTTTTGCTATACTCTGCTTTTAATGATTATGAAG
TTATCTCACTGAAGTCGAAT

Downstream 100 bases:

>100_bases
AAAAACATACTTTTAAGGTTGAAATTTAGAGTAAATACCTGCATAAATACTATTACAAATTAACTTTAAACAGGAATTTC
CTATGGAAGTACACCAGCGT

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSKVKTVYRCEQCGADHLKWAGQCSECGEWNCLNEVKLEPTTAHRARPKVGGYAGQVANITTLNKVSVSHETRLPTGISE
FDRVLGGGLVTGSVVLIGGDPGIGKSTILLQTATHMASAKSSALYITGEESLSQVALRAQRLDLPTDQLKVMAETCVERI
CEVLEQERPVVAILDSIQTLYTETLQSAPGGVSQIRESAALLTRYAKNSGTALFIVGHVTKEGALAGPRVLEHMVDCVLY
FEGQSDSRYRMIRAVKNRFGAVNELGVFGMTDKGLREVANPSAIFLSRYDEAIPGSIVMISREGTRPLLVEVQALVDDAQ
GQPRRVALGLEQNRLNMLLAVMHRHGGVQTTGQDVYVNIVGGLKITETGSDLAVLLACASSLRTKALPQQLAVFGEVGLS
GEIRPVPNGQERLKEAAKHGFKYVILPRGNAPQKAIPGVQVIAVARLHEALTEAMQLSDELT

Sequences:

>Translated_462_residues
MSKVKTVYRCEQCGADHLKWAGQCSECGEWNCLNEVKLEPTTAHRARPKVGGYAGQVANITTLNKVSVSHETRLPTGISE
FDRVLGGGLVTGSVVLIGGDPGIGKSTILLQTATHMASAKSSALYITGEESLSQVALRAQRLDLPTDQLKVMAETCVERI
CEVLEQERPVVAILDSIQTLYTETLQSAPGGVSQIRESAALLTRYAKNSGTALFIVGHVTKEGALAGPRVLEHMVDCVLY
FEGQSDSRYRMIRAVKNRFGAVNELGVFGMTDKGLREVANPSAIFLSRYDEAIPGSIVMISREGTRPLLVEVQALVDDAQ
GQPRRVALGLEQNRLNMLLAVMHRHGGVQTTGQDVYVNIVGGLKITETGSDLAVLLACASSLRTKALPQQLAVFGEVGLS
GEIRPVPNGQERLKEAAKHGFKYVILPRGNAPQKAIPGVQVIAVARLHEALTEAMQLSDELT
>Mature_461_residues
SKVKTVYRCEQCGADHLKWAGQCSECGEWNCLNEVKLEPTTAHRARPKVGGYAGQVANITTLNKVSVSHETRLPTGISEF
DRVLGGGLVTGSVVLIGGDPGIGKSTILLQTATHMASAKSSALYITGEESLSQVALRAQRLDLPTDQLKVMAETCVERIC
EVLEQERPVVAILDSIQTLYTETLQSAPGGVSQIRESAALLTRYAKNSGTALFIVGHVTKEGALAGPRVLEHMVDCVLYF
EGQSDSRYRMIRAVKNRFGAVNELGVFGMTDKGLREVANPSAIFLSRYDEAIPGSIVMISREGTRPLLVEVQALVDDAQG
QPRRVALGLEQNRLNMLLAVMHRHGGVQTTGQDVYVNIVGGLKITETGSDLAVLLACASSLRTKALPQQLAVFGEVGLSG
EIRPVPNGQERLKEAAKHGFKYVILPRGNAPQKAIPGVQVIAVARLHEALTEAMQLSDELT

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=451, Percent_Identity=57.8713968957871, Blast_Score=548, Evalue=1e-157,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49729; Mature: 49598

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVKTVYRCEQCGADHLKWAGQCSECGEWNCLNEVKLEPTTAHRARPKVGGYAGQVANI
CCCHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
TTLNKVSVSHETRLPTGISEFDRVLGGGLVTGSVVLIGGDPGIGKSTILLQTATHMASAK
EEEEEEECCCCCCCCCCHHHHHHHHCCCEEECEEEEEECCCCCCCCEEEEEHHHHHHHCC
SSALYITGEESLSQVALRAQRLDLPTDQLKVMAETCVERICEVLEQERPVVAILDSIQTL
CCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
YTETLQSAPGGVSQIRESAALLTRYAKNSGTALFIVGHVTKEGALAGPRVLEHMVDCVLY
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHEEE
FEGQSDSRYRMIRAVKNRFGAVNELGVFGMTDKGLREVANPSAIFLSRYDEAIPGSIVMI
EECCCCCHHHHHHHHHHHHCCHHHCCEECCCCHHHHHHCCCCEEEEEHHCCCCCCCEEEE
SREGTRPLLVEVQALVDDAQGQPRRVALGLEQNRLNMLLAVMHRHGGVQTTGQDVYVNIV
ECCCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
GGLKITETGSDLAVLLACASSLRTKALPQQLAVFGEVGLSGEIRPVPNGQERLKEAAKHG
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCC
FKYVILPRGNAPQKAIPGVQVIAVARLHEALTEAMQLSDELT
CEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKVKTVYRCEQCGADHLKWAGQCSECGEWNCLNEVKLEPTTAHRARPKVGGYAGQVANI
CCHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
TTLNKVSVSHETRLPTGISEFDRVLGGGLVTGSVVLIGGDPGIGKSTILLQTATHMASAK
EEEEEEECCCCCCCCCCHHHHHHHHCCCEEECEEEEEECCCCCCCCEEEEEHHHHHHHCC
SSALYITGEESLSQVALRAQRLDLPTDQLKVMAETCVERICEVLEQERPVVAILDSIQTL
CCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
YTETLQSAPGGVSQIRESAALLTRYAKNSGTALFIVGHVTKEGALAGPRVLEHMVDCVLY
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHEEE
FEGQSDSRYRMIRAVKNRFGAVNELGVFGMTDKGLREVANPSAIFLSRYDEAIPGSIVMI
EECCCCCHHHHHHHHHHHHCCHHHCCEECCCCHHHHHHCCCCEEEEEHHCCCCCCCEEEE
SREGTRPLLVEVQALVDDAQGQPRRVALGLEQNRLNMLLAVMHRHGGVQTTGQDVYVNIV
ECCCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
GGLKITETGSDLAVLLACASSLRTKALPQQLAVFGEVGLSGEIRPVPNGQERLKEAAKHG
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCC
FKYVILPRGNAPQKAIPGVQVIAVARLHEALTEAMQLSDELT
CEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]