Click here to switch to the map view.

The map label for this gene is tolB [H]

Identifier: 213158279

GI number: 213158279

Start: 3095131

End: 3096411

Strand: Direct

Name: tolB [H]

Synonym: AB57_3002

Alternate gene names: 213158279

Gene position: 3095131-3096411 (Clockwise)

Preceding gene: 213158278

Following gene: 213158280

Centisome position: 76.41

GC content: 40.98

Gene sequence:

>1281_bases
ATGAAAACGAGCCGCAAACACCTTCTAGCCTTAACGCTACTTACAGCATTAAGTCCTCTTGCACCAACAGCTGCCTTTGC
TCAATTACATTTAGAGATTGCAAAAGCACCTGATCAGGCGCCTAAAATCGCTATTGTGCCTTTTAATAACGACAATGGAC
TTTATCCTATTGTTGAAACTGACTTAAACCGCTCAGGACGTTTCACAAGTTCATCAAAAAATTTACCTGCGAATGCCGCT
ATTAACCAGATTCAAGCATCTGATTGGCAAGCTGCGGGTATTCCTTATGTTGTTACTGGACAAATTAAGCAAACAGCTGA
CGGGTTTGAAGTCCATTACCAACTTTATGATGTACAAAAACAGCAATATCTTCTGAATGAGTTGTTAAATGTTCCTGCTT
CACGCATACGTCAAGCAGGACATATGGTAAGTGACGCTATTTATCAGGCCTTAACCGGAATTCCAGGCGATTTTAGTGGT
CGTATCGCTTATGTATTACGTAATCCTGCCACTCCTGCTGAACGTTATACCTTACAAATCGCCGATACAGATGGTGAACA
ACCGAAAACTGTTCTCTCTTCACGTGACCCGATTTTGTCTCCGGCATGGACTCCTGACGCTAAAAAAATTGCTTATGTTT
CTTTTGAAACTAAACGTCCAGCCATTTATTTACAAGATCTCTCTACTGGAACACGTGAAGTACTTACGAGTTTTAAAGGC
TTAAATGGTGCACCAAGCTTTTCACCAGATGGCAAATCAATGCTATTTACAGCATCGATGAATGGTAACCCTGAAATTTA
TCAAATGGATTTATCAACTCGCCAAGTCAAACGCATGACAAATGACTCGGGAATTGATACTGAAGCGCGTTACACACCAG
ATGGAAAAGCTTTTATTTTTACATCTGACCGTGGAGGCTCTCCTCAAATTTATCGCTATGATTTTGGGAATGGCTCAGTA
AAACGCTTAACATTTAAAGGGTCATTTAATGCTCGTGGCACTTTAAGTGCAGATGGTAAAAAAATTGCACTCGTGCATCG
ACCAAGTGGTAGTAACTATAAAGTTGCGATCCAAGATATTAATACAGGCATTGTCAATATTCTTACCCCAACCAGTTTAG
ATGAATCACCAAGTTTCTCACCAAATGGGCAAATGGTGGTTTATGCAACTCGTGAAGGGAACCGTGGTTTACTGTCTATC
ATGTCCACTGATGGTCGTTTCCGTATGAATTTACCGAGTGAACAAGGTGAAGTACGTGAACCAGCCTGGGCACCTAAATA
A

Upstream 100 bases:

>100_bases
ACAATAATTTTGTAAGAGTTACATGCTTTAAATAGTTTGAAGCATGTAACTATGCTACAAAATCACCAGTCACAAAAATA
CCTTCAATGACTTTAAACCT

Downstream 100 bases:

>100_bases
CTTTTTATTCCATTGATGGAGATGATGATGAAATCAATTAAATATTTGGCCTTTCCTCTACTTAGTGCGGCACTTGTCAT
GACAGGTTGTGCAAGTCGTA

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKTSRKHLLALTLLTALSPLAPTAAFAQLHLEIAKAPDQAPKIAIVPFNNDNGLYPIVETDLNRSGRFTSSSKNLPANAA
INQIQASDWQAAGIPYVVTGQIKQTADGFEVHYQLYDVQKQQYLLNELLNVPASRIRQAGHMVSDAIYQALTGIPGDFSG
RIAYVLRNPATPAERYTLQIADTDGEQPKTVLSSRDPILSPAWTPDAKKIAYVSFETKRPAIYLQDLSTGTREVLTSFKG
LNGAPSFSPDGKSMLFTASMNGNPEIYQMDLSTRQVKRMTNDSGIDTEARYTPDGKAFIFTSDRGGSPQIYRYDFGNGSV
KRLTFKGSFNARGTLSADGKKIALVHRPSGSNYKVAIQDINTGIVNILTPTSLDESPSFSPNGQMVVYATREGNRGLLSI
MSTDGRFRMNLPSEQGEVREPAWAPK

Sequences:

>Translated_426_residues
MKTSRKHLLALTLLTALSPLAPTAAFAQLHLEIAKAPDQAPKIAIVPFNNDNGLYPIVETDLNRSGRFTSSSKNLPANAA
INQIQASDWQAAGIPYVVTGQIKQTADGFEVHYQLYDVQKQQYLLNELLNVPASRIRQAGHMVSDAIYQALTGIPGDFSG
RIAYVLRNPATPAERYTLQIADTDGEQPKTVLSSRDPILSPAWTPDAKKIAYVSFETKRPAIYLQDLSTGTREVLTSFKG
LNGAPSFSPDGKSMLFTASMNGNPEIYQMDLSTRQVKRMTNDSGIDTEARYTPDGKAFIFTSDRGGSPQIYRYDFGNGSV
KRLTFKGSFNARGTLSADGKKIALVHRPSGSNYKVAIQDINTGIVNILTPTSLDESPSFSPNGQMVVYATREGNRGLLSI
MSTDGRFRMNLPSEQGEVREPAWAPK
>Mature_426_residues
MKTSRKHLLALTLLTALSPLAPTAAFAQLHLEIAKAPDQAPKIAIVPFNNDNGLYPIVETDLNRSGRFTSSSKNLPANAA
INQIQASDWQAAGIPYVVTGQIKQTADGFEVHYQLYDVQKQQYLLNELLNVPASRIRQAGHMVSDAIYQALTGIPGDFSG
RIAYVLRNPATPAERYTLQIADTDGEQPKTVLSSRDPILSPAWTPDAKKIAYVSFETKRPAIYLQDLSTGTREVLTSFKG
LNGAPSFSPDGKSMLFTASMNGNPEIYQMDLSTRQVKRMTNDSGIDTEARYTPDGKAFIFTSDRGGSPQIYRYDFGNGSV
KRLTFKGSFNARGTLSADGKKIALVHRPSGSNYKVAIQDINTGIVNILTPTSLDESPSFSPNGQMVVYATREGNRGLLSI
MSTDGRFRMNLPSEQGEVREPAWAPK

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=427, Percent_Identity=37.4707259953162, Blast_Score=276, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 46573; Mature: 46573

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSRKHLLALTLLTALSPLAPTAAFAQLHLEIAKAPDQAPKIAIVPFNNDNGLYPIVET
CCCCHHHHHHHHHHHHHCCCCCHHHHHHEEEEEECCCCCCCEEEEEEECCCCCEEEEEEC
DLNRSGRFTSSSKNLPANAAINQIQASDWQAAGIPYVVTGQIKQTADGFEVHYQLYDVQK
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEECCEECCCCCEEEEEEEEHHHH
QQYLLNELLNVPASRIRQAGHMVSDAIYQALTGIPGDFSGRIAYVLRNPATPAERYTLQI
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEE
ADTDGEQPKTVLSSRDPILSPAWTPDAKKIAYVSFETKRPAIYLQDLSTGTREVLTSFKG
ECCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHCC
LNGAPSFSPDGKSMLFTASMNGNPEIYQMDLSTRQVKRMTNDSGIDTEARYTPDGKAFIF
CCCCCCCCCCCCEEEEEEECCCCCCEEEEECCHHHHHHHCCCCCCCCCCEECCCCCEEEE
TSDRGGSPQIYRYDFGNGSVKRLTFKGSFNARGTLSADGKKIALVHRPSGSNYKVAIQDI
ECCCCCCCCEEEEECCCCCEEEEEEECCCCCCEEECCCCCEEEEEECCCCCCEEEEEEEC
NTGIVNILTPTSLDESPSFSPNGQMVVYATREGNRGLLSIMSTDGRFRMNLPSEQGEVRE
CCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCEEEECCCCCCCCCCC
PAWAPK
CCCCCC
>Mature Secondary Structure
MKTSRKHLLALTLLTALSPLAPTAAFAQLHLEIAKAPDQAPKIAIVPFNNDNGLYPIVET
CCCCHHHHHHHHHHHHHCCCCCHHHHHHEEEEEECCCCCCCEEEEEEECCCCCEEEEEEC
DLNRSGRFTSSSKNLPANAAINQIQASDWQAAGIPYVVTGQIKQTADGFEVHYQLYDVQK
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEECCEECCCCCEEEEEEEEHHHH
QQYLLNELLNVPASRIRQAGHMVSDAIYQALTGIPGDFSGRIAYVLRNPATPAERYTLQI
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEE
ADTDGEQPKTVLSSRDPILSPAWTPDAKKIAYVSFETKRPAIYLQDLSTGTREVLTSFKG
ECCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHCC
LNGAPSFSPDGKSMLFTASMNGNPEIYQMDLSTRQVKRMTNDSGIDTEARYTPDGKAFIF
CCCCCCCCCCCCEEEEEEECCCCCCEEEEECCHHHHHHHCCCCCCCCCCEECCCCCEEEE
TSDRGGSPQIYRYDFGNGSVKRLTFKGSFNARGTLSADGKKIALVHRPSGSNYKVAIQDI
ECCCCCCCCEEEEECCCCCEEEEEEECCCCCCEEECCCCCEEEEEECCCCCCEEEEEEEC
NTGIVNILTPTSLDESPSFSPNGQMVVYATREGNRGLLSIMSTDGRFRMNLPSEQGEVRE
CCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCEEEECCCCCCCCCCC
PAWAPK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA