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The map label for this gene is bcr [H]

Identifier: 213158270

GI number: 213158270

Start: 3081879

End: 3083084

Strand: Reverse

Name: bcr [H]

Synonym: AB57_2991

Alternate gene names: 213158270

Gene position: 3083084-3081879 (Counterclockwise)

Preceding gene: 213158271

Following gene: 213158268

Centisome position: 76.12

GC content: 38.14

Gene sequence:

>1206_bases
ATGAAGTCAAAAATCCATTTCCGTGAGTTTGCACTTTTGATGGCATTACTCATGTCTATCGTGTCTTTTTCGATAGATGC
AGTGTTGCCTGCTTTAGGTGAGGTTGGACGAGTTTTTGAATTAAAGAATAATAATCAGACCCAGTGGGTCATTATTGGTA
TTTTCTCCGGCATGACAATAGGGCAGTTGATTGCAGGTCCACTTTCCGATGCGATTGGACGAAAACGTATTTTATTTACC
GGTATTATCATCTACTTTTTGGGAAGTTTATTGTGCTTTACCACACAAAGTTTTGAATGGTTTTTAGTAGGTCGCTTTAT
TCAAGGTATTGGGGTTTCAGGTCCTTATGTCGCCTCAATTTCCATTGTGCGAGATAAATACAGCGGCGCTCAGATGGCCC
GTATTATGTCCTTAATCATGATGGTATTTATGGTCGCTCCAGCGATTGCACCAAGTCTGGGACAACTCATCATTCATTTC
TTTGGTTGGCGTGATATTTTTGTTTTATATATGATCTATGCCACAGTCGTTGGAGTGTGGGTCGCTTTACGATTAGAAGA
AACGTTGCTACCAGAAAACCGTTTACCTATGCGTTTACAAGCATTTCAAGAGGGTTTCAAAGAAATTGTAAGTAATAAAA
CCACCATGAGCTATTTGTTATGTGCAGGCTTTTGCTTTGGGGGATTTATTGGTTACTTAGGTACTTCTCAGCAAATTTTT
ATGCAGCAGTTTGGTAAAACCGGGCAAGAGTTTAGTGCTTATTTTGCTGTGCTTGCTGGTGTAATGGGCATTGCTTCATT
TACCAATTCTAAAATTGTCATGAAGTTTGGTATGCGTCCAATCTGTATTTATGGCTTTCTCGGTCTGTGCTTAATTTCAT
TAATTTTCTTAGGGATTCAACTTATAGGTGTGACTGTAGCATTTTGGATGTTTATGCTTTATGCCTGTATTTTATTTTTA
TTATTTGGAACTTTATTCGGTAATTTAAACGCGATTGCAATGGAACCTATGGGACATGTAGCTGGTATGGCATCAGCTAT
TATTGGTGCAGCTTCGTCCGTATTGTCACTTATTCTTGCCTCTATTATTGGGCAATTATATAACGGCACATTAATACCAA
TGACTGGCGGCTTCGTCATTTTATGTGGTTTAGCATTTATGATGACCGTATATGAGAACAGATATTTAAAGAAATCGAAT
GTTTAA

Upstream 100 bases:

>100_bases
CGTAAGTTTATTGGTTAATATAAAAATTGAAATAAGCCACCGTAAGGTGGCTTATTTGTATGTAGTACAATGTAAGTAGA
ATAAATAGTAGAGAAGAAAA

Downstream 100 bases:

>100_bases
AATGATCCAGAATAATGATAGCTCACTGAATTAGAAAAATTTTTTTTAAACTTAAAGTAAAGGTAAGAAAATACGTTTTT
TAAGGACTTTAAGAAAATCT

Product: MFS family drug transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MKSKIHFREFALLMALLMSIVSFSIDAVLPALGEVGRVFELKNNNQTQWVIIGIFSGMTIGQLIAGPLSDAIGRKRILFT
GIIIYFLGSLLCFTTQSFEWFLVGRFIQGIGVSGPYVASISIVRDKYSGAQMARIMSLIMMVFMVAPAIAPSLGQLIIHF
FGWRDIFVLYMIYATVVGVWVALRLEETLLPENRLPMRLQAFQEGFKEIVSNKTTMSYLLCAGFCFGGFIGYLGTSQQIF
MQQFGKTGQEFSAYFAVLAGVMGIASFTNSKIVMKFGMRPICIYGFLGLCLISLIFLGIQLIGVTVAFWMFMLYACILFL
LFGTLFGNLNAIAMEPMGHVAGMASAIIGAASSVLSLILASIIGQLYNGTLIPMTGGFVILCGLAFMMTVYENRYLKKSN
V

Sequences:

>Translated_401_residues
MKSKIHFREFALLMALLMSIVSFSIDAVLPALGEVGRVFELKNNNQTQWVIIGIFSGMTIGQLIAGPLSDAIGRKRILFT
GIIIYFLGSLLCFTTQSFEWFLVGRFIQGIGVSGPYVASISIVRDKYSGAQMARIMSLIMMVFMVAPAIAPSLGQLIIHF
FGWRDIFVLYMIYATVVGVWVALRLEETLLPENRLPMRLQAFQEGFKEIVSNKTTMSYLLCAGFCFGGFIGYLGTSQQIF
MQQFGKTGQEFSAYFAVLAGVMGIASFTNSKIVMKFGMRPICIYGFLGLCLISLIFLGIQLIGVTVAFWMFMLYACILFL
LFGTLFGNLNAIAMEPMGHVAGMASAIIGAASSVLSLILASIIGQLYNGTLIPMTGGFVILCGLAFMMTVYENRYLKKSN
V
>Mature_401_residues
MKSKIHFREFALLMALLMSIVSFSIDAVLPALGEVGRVFELKNNNQTQWVIIGIFSGMTIGQLIAGPLSDAIGRKRILFT
GIIIYFLGSLLCFTTQSFEWFLVGRFIQGIGVSGPYVASISIVRDKYSGAQMARIMSLIMMVFMVAPAIAPSLGQLIIHF
FGWRDIFVLYMIYATVVGVWVALRLEETLLPENRLPMRLQAFQEGFKEIVSNKTTMSYLLCAGFCFGGFIGYLGTSQQIF
MQQFGKTGQEFSAYFAVLAGVMGIASFTNSKIVMKFGMRPICIYGFLGLCLISLIFLGIQLIGVTVAFWMFMLYACILFL
LFGTLFGNLNAIAMEPMGHVAGMASAIIGAASSVLSLILASIIGQLYNGTLIPMTGGFVILCGLAFMMTVYENRYLKKSN
V

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=400, Percent_Identity=23.75, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1790146, Length=320, Percent_Identity=26.5625, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI48994889, Length=230, Percent_Identity=30.4347826086957, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082312, Length=367, Percent_Identity=25.0681198910082, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790794, Length=313, Percent_Identity=24.6006389776358, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1787065, Length=224, Percent_Identity=25, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44116; Mature: 44116

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKSKIHFREFALLMALLMSIVSFSIDAVLPALGEVGRVFELKNNNQTQWVIIGIFSGMTI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEEEECCCCHH
GQLIAGPLSDAIGRKRILFTGIIIYFLGSLLCFTTQSFEWFLVGRFIQGIGVSGPYVASI
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHH
SIVRDKYSGAQMARIMSLIMMVFMVAPAIAPSLGQLIIHFFGWRDIFVLYMIYATVVGVW
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VALRLEETLLPENRLPMRLQAFQEGFKEIVSNKTTMSYLLCAGFCFGGFIGYLGTSQQIF
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
MQQFGKTGQEFSAYFAVLAGVMGIASFTNSKIVMKFGMRPICIYGFLGLCLISLIFLGIQ
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHCCCHHHHHHHHHHHHHHHHHHHHH
LIGVTVAFWMFMLYACILFLLFGTLFGNLNAIAMEPMGHVAGMASAIIGAASSVLSLILA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHH
SIIGQLYNGTLIPMTGGFVILCGLAFMMTVYENRYLKKSNV
HHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]