Click here to switch to the map view.

The map label for this gene is paaF [H]

Identifier: 213158269

GI number: 213158269

Start: 3080370

End: 3081170

Strand: Direct

Name: paaF [H]

Synonym: AB57_2989

Alternate gene names: 213158269

Gene position: 3080370-3081170 (Clockwise)

Preceding gene: 213158266

Following gene: 213158272

Centisome position: 76.05

GC content: 40.07

Gene sequence:

>801_bases
ATGACACTTAGCTGTATTCAACAACCTCATCAACATTTACAAGCAAATTTAGAAGGTGGCGTACTCACCTTAGCCATAAA
TCGCCCAGAAGCAAAAAATGCATTATATGGCGAACTTTACTTATGGATTGCCAAAGCACTTGATGAAGCCGACCAAAATA
AAGATGTACGAGTAGTCGTTCTACGTGGTGCCGAACATGACTTTACCGCTGGTAATGACATGAAAGATTTTATGGGTTTT
GTACAAAATCCGAATGCTGGTCCAGCAGGTCAAGTTCCTCCATTTGTACTCCTTAAATCTGCTGCCAGATTATCGAAGCC
GCTTATTATTGCAGTAAAAGGCGTGGCAATTGGTATTGGTGTTACGATTTTATTACAGGCAGACCTCGTTTTTGCCGACA
ATACGGCCCTCTTTCAAATTCCTTTTGTTAGCCTTGGGCTTTCTCCTGAAGGTGGTGCAAGTCAATTATTGGTTAAACAA
GCTGGTTATCACAAAGCAGCTGAACTTCTGTTTACTGCGAAAAAGTTCAATGCAGAAGCTGCTGTACAAGCTGGTTTAGT
GAATGAAATCGTTGAAGATGCTTATGCAACTGCTCAGGCAACTGCTCAACACTTAACAGCGTTACCGCTTGCTTCATTAA
AACAAACCAAAGCATTAATGAAACATGATTTAGATCAGATTATTGAATGTATTGACCATGAAGCAGAAATCTTTATGCAA
CGCGTTCAATCACCAGAAATGCTAGAAGCTGTGCAGGCATTTATGCAAAAACGTAAACCGGACTTTTCACAGTTTAATTA
A

Upstream 100 bases:

>100_bases
TTCACTTTTTATGAACTCACCAGTTACGCTCATTACAAAAAATATGCGCCATAATGAGAACCTAAAAATCAACAACGTGG
TTTAAAGCAAGGATAGTACG

Downstream 100 bases:

>100_bases
AAATCAACTTCTATGATTTGAATAAACTCAAAGGCAGGCTCTTCGTAAGGATGGCTTGCCTTTAATGCTTTTGCCACTGC
ACTTGCCTTTTCTTCAAGCA

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MTLSCIQQPHQHLQANLEGGVLTLAINRPEAKNALYGELYLWIAKALDEADQNKDVRVVVLRGAEHDFTAGNDMKDFMGF
VQNPNAGPAGQVPPFVLLKSAARLSKPLIIAVKGVAIGIGVTILLQADLVFADNTALFQIPFVSLGLSPEGGASQLLVKQ
AGYHKAAELLFTAKKFNAEAAVQAGLVNEIVEDAYATAQATAQHLTALPLASLKQTKALMKHDLDQIIECIDHEAEIFMQ
RVQSPEMLEAVQAFMQKRKPDFSQFN

Sequences:

>Translated_266_residues
MTLSCIQQPHQHLQANLEGGVLTLAINRPEAKNALYGELYLWIAKALDEADQNKDVRVVVLRGAEHDFTAGNDMKDFMGF
VQNPNAGPAGQVPPFVLLKSAARLSKPLIIAVKGVAIGIGVTILLQADLVFADNTALFQIPFVSLGLSPEGGASQLLVKQ
AGYHKAAELLFTAKKFNAEAAVQAGLVNEIVEDAYATAQATAQHLTALPLASLKQTKALMKHDLDQIIECIDHEAEIFMQ
RVQSPEMLEAVQAFMQKRKPDFSQFN
>Mature_265_residues
TLSCIQQPHQHLQANLEGGVLTLAINRPEAKNALYGELYLWIAKALDEADQNKDVRVVVLRGAEHDFTAGNDMKDFMGFV
QNPNAGPAGQVPPFVLLKSAARLSKPLIIAVKGVAIGIGVTILLQADLVFADNTALFQIPFVSLGLSPEGGASQLLVKQA
GYHKAAELLFTAKKFNAEAAVQAGLVNEIVEDAYATAQATAQHLTALPLASLKQTKALMKHDLDQIIECIDHEAEIFMQR
VQSPEMLEAVQAFMQKRKPDFSQFN

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI260274832, Length=248, Percent_Identity=31.0483870967742, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI45643119, Length=248, Percent_Identity=31.0483870967742, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI260275230, Length=248, Percent_Identity=31.0483870967742, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI70995211, Length=253, Percent_Identity=26.8774703557312, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI25453481, Length=240, Percent_Identity=26.6666666666667, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI51538573, Length=240, Percent_Identity=26.6666666666667, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4757968, Length=240, Percent_Identity=26.25, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI49355787, Length=240, Percent_Identity=26.25, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI194097323, Length=243, Percent_Identity=28.3950617283951, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI4757966, Length=229, Percent_Identity=27.5109170305677, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI51538581, Length=229, Percent_Identity=27.5109170305677, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI221136756, Length=238, Percent_Identity=26.4705882352941, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI221136753, Length=238, Percent_Identity=26.4705882352941, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI221307494, Length=238, Percent_Identity=26.4705882352941, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI116235487, Length=250, Percent_Identity=25.6, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=26.5625, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI68989263, Length=234, Percent_Identity=28.6324786324786, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1787659, Length=248, Percent_Identity=30.6451612903226, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=32.5670498084291, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1788597, Length=253, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082183, Length=229, Percent_Identity=27.0742358078603, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=257, Percent_Identity=34.6303501945525, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=27.0588235294118, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=258, Percent_Identity=28.2945736434109, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=209, Percent_Identity=30.1435406698565, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=180, Percent_Identity=31.1111111111111, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=266, Percent_Identity=25.187969924812, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=263, Percent_Identity=23.574144486692, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323314, Length=204, Percent_Identity=25.9803921568627, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24654903, Length=260, Percent_Identity=30, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI20129971, Length=250, Percent_Identity=28, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24653477, Length=250, Percent_Identity=28, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19920382, Length=261, Percent_Identity=29.1187739463602, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24653139, Length=257, Percent_Identity=27.6264591439689, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24650670, Length=215, Percent_Identity=24.6511627906977, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28885; Mature: 28753

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSCIQQPHQHLQANLEGGVLTLAINRPEAKNALYGELYLWIAKALDEADQNKDVRVVV
CCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEE
LRGAEHDFTAGNDMKDFMGFVQNPNAGPAGQVPPFVLLKSAARLSKPLIIAVKGVAIGIG
EECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCHHHHH
VTILLQADLVFADNTALFQIPFVSLGLSPEGGASQLLVKQAGYHKAAELLFTAKKFNAEA
EEEEEEECEEEECCCEEEEECEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCHHH
AVQAGLVNEIVEDAYATAQATAQHLTALPLASLKQTKALMKHDLDQIIECIDHEAEIFMQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RVQSPEMLEAVQAFMQKRKPDFSQFN
HCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TLSCIQQPHQHLQANLEGGVLTLAINRPEAKNALYGELYLWIAKALDEADQNKDVRVVV
CCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEE
LRGAEHDFTAGNDMKDFMGFVQNPNAGPAGQVPPFVLLKSAARLSKPLIIAVKGVAIGIG
EECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCHHHHH
VTILLQADLVFADNTALFQIPFVSLGLSPEGGASQLLVKQAGYHKAAELLFTAKKFNAEA
EEEEEEECEEEECCCEEEEECEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCHHH
AVQAGLVNEIVEDAYATAQATAQHLTALPLASLKQTKALMKHDLDQIIECIDHEAEIFMQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RVQSPEMLEAVQAFMQKRKPDFSQFN
HCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]