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The map label for this gene is purB [H]

Identifier: 213158154

GI number: 213158154

Start: 2955297

End: 2956604

Strand: Direct

Name: purB [H]

Synonym: AB57_2868

Alternate gene names: 213158154

Gene position: 2955297-2956604 (Clockwise)

Preceding gene: 213158153

Following gene: 213158155

Centisome position: 72.96

GC content: 39.83

Gene sequence:

>1308_bases
ATGAACGCTTTAACCGCACTATCACCATTAGATGGACGTTATGCCAGCAAATGTGATGCGCTACGCCCTTTTCTTTCTGA
GTTTGGTTTAATTCATGCTCGTGTCACTGTGGAAGTGCGTTGGTTACAAGCGCTTTCTAACCGTCCGGAAATTGTTGAAG
TTGCTCCTTTCTCAACTGAAACAAATGCAGCTCTAGATGCAATCGTAAGCAACTTCTCTGAAGAAGATGCGAACCGCATT
AAAGAAATTGAACGCACAACTAACCATGACGTAAAAGCAGTTGAATACTTCTTGAAAGAGAAAATTGCAGGTATTGCTGA
ATTACAAAATGCAGGTGAGTTCATTCACTTTGCTTGTACATCAGAAGACATCAATAACTTGTCTCATGCACTTATGCTTA
AAAACGGTCGTGAAGTTTTAGTATCAAGCATGAAGCAAATTTTAAATGCAATCTCTGCTTTAGCTACAACTCATGCTGAA
CAACCGATGTTGTCTCGTACACATGGTCAAACTGCTAGCCCAACAACTTTGGGTAAAGAAATGGCGAACGTTGCATATCG
TTTAGCTCGCCAAATCAAACAATTTGAAAATGTTGAATTATTAGGCAAAATCAATGGTGCAGTAGGTAACTACAATGCTC
ACCTTTCTGCTTATCCAAATGTGGATTGGCCAGCGCATTCACAAGCTTTTGTTGAATCTTTAGGTTTAACATTTAATCCA
TATACAACTCAAATTGAGCCACACGACTACATGGCTGAATTATTTGACGCGTTACGTCGTTTCAATACAATTTTGATCGA
CTTTAACCGTGACGTATGGGGTTATATCTCTTTAGGCTACTTCAAGCAAAAACTTAAAGAAGGTGAAGTTGGCTCATCAA
CTATGCCACATAAAGTTAACCCGATCGACTTTGAAAACTCTGAAGGTAACTTAGGCATTGCAAATGCAGTGTTGGCTCAC
TTAGGCGAAAAATTACCAATTTCTCGCTGGCAGCGTGACTTAACTGACTCAACTGTACTTCGTAACATGGGTGTTGGTTT
TGCTCAAAGCTTAATTGCTTTTGATGCTTGCTTAAAAGGTATTGGTAAACTTGAACTCAATGCAAACCGCTTAAATGAAG
ATCTTGACCAAGCTCAAGAAGTTCTTGCAGAGCCAATTCAAACAGTTATGCGTCGTTATAACGTTGAAAAACCATATGAG
AAGTTAAAAGCATTAACTCGTGGTCAAGCAATGACGCGTGACATGATGGTTAACTTCGTAAATGGTAACGAACTGCTCAA
GTACCAAGTGAAGAGCGCGCCCGCTTAG

Upstream 100 bases:

>100_bases
ACAGGATATTCAAGCACTTGCTCGTTTACAGTACCAAGTTATCTAAGGCTGTAACGAGATTATTTTTGTTTTATGTTTAT
TCCAGCATTAAGGAATCGTT

Downstream 100 bases:

>100_bases
CTGAGCTTACACCTGCAACTTACACAGGTAATGCTGCTGAACAAGCTAAACAAATTAACGAATTAATTAGCAAAATCTAA
TTAAAAAAGTTATAAAAAGC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MNALTALSPLDGRYASKCDALRPFLSEFGLIHARVTVEVRWLQALSNRPEIVEVAPFSTETNAALDAIVSNFSEEDANRI
KEIERTTNHDVKAVEYFLKEKIAGIAELQNAGEFIHFACTSEDINNLSHALMLKNGREVLVSSMKQILNAISALATTHAE
QPMLSRTHGQTASPTTLGKEMANVAYRLARQIKQFENVELLGKINGAVGNYNAHLSAYPNVDWPAHSQAFVESLGLTFNP
YTTQIEPHDYMAELFDALRRFNTILIDFNRDVWGYISLGYFKQKLKEGEVGSSTMPHKVNPIDFENSEGNLGIANAVLAH
LGEKLPISRWQRDLTDSTVLRNMGVGFAQSLIAFDACLKGIGKLELNANRLNEDLDQAQEVLAEPIQTVMRRYNVEKPYE
KLKALTRGQAMTRDMMVNFVNGNELLKYQVKSAPA

Sequences:

>Translated_435_residues
MNALTALSPLDGRYASKCDALRPFLSEFGLIHARVTVEVRWLQALSNRPEIVEVAPFSTETNAALDAIVSNFSEEDANRI
KEIERTTNHDVKAVEYFLKEKIAGIAELQNAGEFIHFACTSEDINNLSHALMLKNGREVLVSSMKQILNAISALATTHAE
QPMLSRTHGQTASPTTLGKEMANVAYRLARQIKQFENVELLGKINGAVGNYNAHLSAYPNVDWPAHSQAFVESLGLTFNP
YTTQIEPHDYMAELFDALRRFNTILIDFNRDVWGYISLGYFKQKLKEGEVGSSTMPHKVNPIDFENSEGNLGIANAVLAH
LGEKLPISRWQRDLTDSTVLRNMGVGFAQSLIAFDACLKGIGKLELNANRLNEDLDQAQEVLAEPIQTVMRRYNVEKPYE
KLKALTRGQAMTRDMMVNFVNGNELLKYQVKSAPA
>Mature_435_residues
MNALTALSPLDGRYASKCDALRPFLSEFGLIHARVTVEVRWLQALSNRPEIVEVAPFSTETNAALDAIVSNFSEEDANRI
KEIERTTNHDVKAVEYFLKEKIAGIAELQNAGEFIHFACTSEDINNLSHALMLKNGREVLVSSMKQILNAISALATTHAE
QPMLSRTHGQTASPTTLGKEMANVAYRLARQIKQFENVELLGKINGAVGNYNAHLSAYPNVDWPAHSQAFVESLGLTFNP
YTTQIEPHDYMAELFDALRRFNTILIDFNRDVWGYISLGYFKQKLKEGEVGSSTMPHKVNPIDFENSEGNLGIANAVLAH
LGEKLPISRWQRDLTDSTVLRNMGVGFAQSLIAFDACLKGIGKLELNANRLNEDLDQAQEVLAEPIQTVMRRYNVEKPYE
KLKALTRGQAMTRDMMVNFVNGNELLKYQVKSAPA

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=423, Percent_Identity=66.193853427896, Blast_Score=594, Evalue=1e-171,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48532; Mature: 48532

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALTALSPLDGRYASKCDALRPFLSEFGLIHARVTVEVRWLQALSNRPEIVEVAPFSTE
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEHHHHHHHHCCCCCEEEECCCCCC
TNAALDAIVSNFSEEDANRIKEIERTTNHDVKAVEYFLKEKIAGIAELQNAGEFIHFACT
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
SEDINNLSHALMLKNGREVLVSSMKQILNAISALATTHAEQPMLSRTHGQTASPTTLGKE
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHH
MANVAYRLARQIKQFENVELLGKINGAVGNYNAHLSAYPNVDWPAHSQAFVESLGLTFNP
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHCCCCCCC
YTTQIEPHDYMAELFDALRRFNTILIDFNRDVWGYISLGYFKQKLKEGEVGSSTMPHKVN
CCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PIDFENSEGNLGIANAVLAHLGEKLPISRWQRDLTDSTVLRNMGVGFAQSLIAFDACLKG
CCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
IGKLELNANRLNEDLDQAQEVLAEPIQTVMRRYNVEKPYEKLKALTRGQAMTRDMMVNFV
CCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEC
NGNELLKYQVKSAPA
CCCCEEEEECCCCCC
>Mature Secondary Structure
MNALTALSPLDGRYASKCDALRPFLSEFGLIHARVTVEVRWLQALSNRPEIVEVAPFSTE
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEHHHHHHHHCCCCCEEEECCCCCC
TNAALDAIVSNFSEEDANRIKEIERTTNHDVKAVEYFLKEKIAGIAELQNAGEFIHFACT
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
SEDINNLSHALMLKNGREVLVSSMKQILNAISALATTHAEQPMLSRTHGQTASPTTLGKE
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHH
MANVAYRLARQIKQFENVELLGKINGAVGNYNAHLSAYPNVDWPAHSQAFVESLGLTFNP
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHCCCCCCC
YTTQIEPHDYMAELFDALRRFNTILIDFNRDVWGYISLGYFKQKLKEGEVGSSTMPHKVN
CCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PIDFENSEGNLGIANAVLAHLGEKLPISRWQRDLTDSTVLRNMGVGFAQSLIAFDACLKG
CCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
IGKLELNANRLNEDLDQAQEVLAEPIQTVMRRYNVEKPYEKLKALTRGQAMTRDMMVNFV
CCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEC
NGNELLKYQVKSAPA
CCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]