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The map label for this gene is dacA [H]

Identifier: 213158149

GI number: 213158149

Start: 2950955

End: 2952103

Strand: Direct

Name: dacA [H]

Synonym: AB57_2863

Alternate gene names: 213158149

Gene position: 2950955-2952103 (Clockwise)

Preceding gene: 213158148

Following gene: 213158150

Centisome position: 72.85

GC content: 37.34

Gene sequence:

>1149_bases
ATGACTCGAAAAAGCGCTATTGCTGCACTCCTCCTCTTACCAAGTTTTTCTTATGCAGCAACTGTCTTATCAGCTCCGCC
AGAATTAAATAATAAGTCTTATGTATTAATGGATTACGAGACAGGACAAATTCTCGCTTCTAAAAATGAAAATGAAAAGC
TTGCTCCTGCTTCTATGACTAAAATGATGACAAGCTACATCATTGAACAAAAATTATTAAAAGGTGAGCTTACCGAAAAT
GAACAGGTTCGTATGAACGAATCTGCTTGGTGTAAAGGCAGTAGTTCTGAATCATGTATGTATGTTCCATTAAATGGTAC
AGCAACTGTTTTAGAAATGCTTCGCGGTATTATTATTCAGTCAGGTAATGATGCATCAAAAGCAATGGCTGAACATATTG
CTGGAAATGAAGGTACTTTTGCTCACATGATGAACCAAGAAGCAAAACGTATCGGAATGACCAATACCCAGTTTATTAAC
TCAACTGGTATGCCAGCTGAAGGTCATTACTCAACTGCAAAAGATATGGCTGTTTTAGCACAACATATTATTAAAGACAG
CTCAAAATACTACCCGATTTATTCTGAAAAAGAATTCACATTTAACGGTATCAAACAAGGTAACCGTAATGCTTTACTTT
ACACTGACCCAAGTGTTGATGGCTTAAAAACAGGCCATACTGATGAAGCTGGTTACTGCTTAACGACTTCAAGTAAACGC
GGTCCAATGCGTTTAATCTCAGTTATTTTTGGTACACCAAGCATGTCTGAACGTGCTGACCAAACTCGTGCATTATTGGC
ATGGGGTTTTGCAAACTTTGAAACTGCAAATGTACAACCTGCAAACCAAGTTCTTGCAAAAGCTAAAGTTTGGTTTGGTA
AAGAAAATGAAGTTCAGGTTGGTTTAGCTGAAAACTTTAATGTGACTATGCCTAAAGGCAAAGCGGATGGCATTAAAACT
CAATTGGTTGTTCAACCGAACCTAAATGCACCACTTCAAAAAGGTCAAGTCGTTGGTAAGCTTGTTGCTAGTTTAGATGG
TAAAGTTATTGCTGAAAAACCATTGGTTGCTTTAAAACCTGTTGAAGAAGCTGGTTTCTTCGCACGTTTAATTGACCATA
TCAAACAATTCTTCAGCAACTTATTCTAA

Upstream 100 bases:

>100_bases
TACTGTTTGAAAAAGCCGACTTCCCCCATAGAAGTCGGCTTTGCTTTATCCTAAAATGCTAGGCTCTTCTGCTTCATACA
AGATATTGGAATTACCTAGA

Downstream 100 bases:

>100_bases
ATTTAAATAATTTTAGAATAGTTGAATTAAGCACTGATGCTATTAGCGTGAGTGCTTTTTTATTTTATACTTTTACTAAA
TTTTCTATTGATACTTCTCA

Product: penicillin-binding protein 6 (D-alanyl-D-alanine carboxypeptidase)

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MTRKSAIAALLLLPSFSYAATVLSAPPELNNKSYVLMDYETGQILASKNENEKLAPASMTKMMTSYIIEQKLLKGELTEN
EQVRMNESAWCKGSSSESCMYVPLNGTATVLEMLRGIIIQSGNDASKAMAEHIAGNEGTFAHMMNQEAKRIGMTNTQFIN
STGMPAEGHYSTAKDMAVLAQHIIKDSSKYYPIYSEKEFTFNGIKQGNRNALLYTDPSVDGLKTGHTDEAGYCLTTSSKR
GPMRLISVIFGTPSMSERADQTRALLAWGFANFETANVQPANQVLAKAKVWFGKENEVQVGLAENFNVTMPKGKADGIKT
QLVVQPNLNAPLQKGQVVGKLVASLDGKVIAEKPLVALKPVEEAGFFARLIDHIKQFFSNLF

Sequences:

>Translated_382_residues
MTRKSAIAALLLLPSFSYAATVLSAPPELNNKSYVLMDYETGQILASKNENEKLAPASMTKMMTSYIIEQKLLKGELTEN
EQVRMNESAWCKGSSSESCMYVPLNGTATVLEMLRGIIIQSGNDASKAMAEHIAGNEGTFAHMMNQEAKRIGMTNTQFIN
STGMPAEGHYSTAKDMAVLAQHIIKDSSKYYPIYSEKEFTFNGIKQGNRNALLYTDPSVDGLKTGHTDEAGYCLTTSSKR
GPMRLISVIFGTPSMSERADQTRALLAWGFANFETANVQPANQVLAKAKVWFGKENEVQVGLAENFNVTMPKGKADGIKT
QLVVQPNLNAPLQKGQVVGKLVASLDGKVIAEKPLVALKPVEEAGFFARLIDHIKQFFSNLF
>Mature_381_residues
TRKSAIAALLLLPSFSYAATVLSAPPELNNKSYVLMDYETGQILASKNENEKLAPASMTKMMTSYIIEQKLLKGELTENE
QVRMNESAWCKGSSSESCMYVPLNGTATVLEMLRGIIIQSGNDASKAMAEHIAGNEGTFAHMMNQEAKRIGMTNTQFINS
TGMPAEGHYSTAKDMAVLAQHIIKDSSKYYPIYSEKEFTFNGIKQGNRNALLYTDPSVDGLKTGHTDEAGYCLTTSSKRG
PMRLISVIFGTPSMSERADQTRALLAWGFANFETANVQPANQVLAKAKVWFGKENEVQVGLAENFNVTMPKGKADGIKTQ
LVVQPNLNAPLQKGQVVGKLVASLDGKVIAEKPLVALKPVEEAGFFARLIDHIKQFFSNLF

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=367, Percent_Identity=41.6893732970027, Blast_Score=311, Evalue=4e-86,
Organism=Escherichia coli, GI1787062, Length=380, Percent_Identity=40.7894736842105, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI87082022, Length=386, Percent_Identity=36.7875647668394, Blast_Score=260, Evalue=9e-71,
Organism=Escherichia coli, GI87082054, Length=268, Percent_Identity=29.8507462686567, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 41783; Mature: 41652

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKSAIAALLLLPSFSYAATVLSAPPELNNKSYVLMDYETGQILASKNENEKLAPASMT
CCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEEEECCCEEEECCCCCCCCCHHHHH
KMMTSYIIEQKLLKGELTENEQVRMNESAWCKGSSSESCMYVPLNGTATVLEMLRGIIIQ
HHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHEEE
SGNDASKAMAEHIAGNEGTFAHMMNQEAKRIGMTNTQFINSTGMPAEGHYSTAKDMAVLA
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHH
QHIIKDSSKYYPIYSEKEFTFNGIKQGNRNALLYTDPSVDGLKTGHTDEAGYCLTTSSKR
HHHHHCCCCCCCEECCCCCEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCC
GPMRLISVIFGTPSMSERADQTRALLAWGFANFETANVQPANQVLAKAKVWFGKENEVQV
CHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHEECCCCCEEE
GLAENFNVTMPKGKADGIKTQLVVQPNLNAPLQKGQVVGKLVASLDGKVIAEKPLVALKP
EEECCCEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCCEEECC
VEEAGFFARLIDHIKQFFSNLF
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRKSAIAALLLLPSFSYAATVLSAPPELNNKSYVLMDYETGQILASKNENEKLAPASMT
CCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEEEECCCEEEECCCCCCCCCHHHHH
KMMTSYIIEQKLLKGELTENEQVRMNESAWCKGSSSESCMYVPLNGTATVLEMLRGIIIQ
HHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHEEE
SGNDASKAMAEHIAGNEGTFAHMMNQEAKRIGMTNTQFINSTGMPAEGHYSTAKDMAVLA
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHH
QHIIKDSSKYYPIYSEKEFTFNGIKQGNRNALLYTDPSVDGLKTGHTDEAGYCLTTSSKR
HHHHHCCCCCCCEECCCCCEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCC
GPMRLISVIFGTPSMSERADQTRALLAWGFANFETANVQPANQVLAKAKVWFGKENEVQV
CHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHEECCCCCEEE
GLAENFNVTMPKGKADGIKTQLVVQPNLNAPLQKGQVVGKLVASLDGKVIAEKPLVALKP
EEECCCEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCCEEECC
VEEAGFFARLIDHIKQFFSNLF
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]